Restriction map for pF1KB0407
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AflIII      
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbsI        BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BpuAI       
BsaBI       BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstYI       BstZ17I     CfoI        
ClaI        CpoI        DrdI        EaeI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KspI        
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SspBI       SspI        SwaI        
Tth111I     XbaI        XcmI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AatII         727  GACGTC               GACGT^C
Acc65I        941  GGTACC               G^GTACC
AcsI          619  GAATTT               [GA]^AATT[CT]
AcyI          727  GACGTC               G[GA]^CG[CT]C
AgeI          938  ACCGGT               A^CCGGT
AhdI          528  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        720  GTGCAC               G^TGCAC
AlwI          183  GGATC                GGATC(4/5)
AlwNI         498  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1148  CCTCAGG              CC^T[ACGT]AGG
ApaLI         720  GTGCAC               G^TGCAC
ApoI          619  GAATTT               [GA]^AATT[CT]
Asp718I       941  GGTACC               G^GTACC
AspEI         528  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI          941  GGTACC               G^G[CT][GA]CC
BbrPI         769  CACGTG               CAC^GTG
BfaI          611  CTAG                 C^TAG
BglI         1192  GCCGAGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           91  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I       91  GCTAAGC              GC^T[ACGT]AGC
BsaHI         727  GACGTC               G[GA]^CG[CT]C
BsaI          213  GAGACC               GGTCTC(1/5)
BseRI         961  GAGGAG               GAGGAG(10/8)
BsrDI        1064  GCAATG               GCAATG(2/0)
BsrFI         938  ACCGGT               [GA]^CCGG[CT]
Bsu36I       1148  CCTCAGG              CC^T[ACGT]AGG
CelII          91  GCTAAGC              GC^T[ACGT]AGC
Cfr10I        938  ACCGGT               [GA]^CCGG[CT]
DraI          628  TTTAAA               TTT^AAA
DraII         453  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII        202  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      528  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          925  GAAGAG               CTCTTC(1/4)
Eco81I       1148  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      453  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I        30  CCATGG               C^C[AT][AT]GG
HgaI         1364  GCGTC                GACGC(5/10)
Hin1I         727  GACGTC               G[GA]^CG[CT]C
KpnI          941  GGTACC               GGTAC^C
Ksp632I       925  GAAGAG               CTCTTC(1/4)
MaeI          611  CTAG                 C^TAG
MslI         1293  CATGCACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI           30  CCATGG               C^CATGG
NspI         1292  GCATGC               [GA]CATG^[CT]
PaeR7I       1327  CTCGAG               C^TCGAG
PflMI         394  CCACCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         938  ACCGGT               A^CCGGT
PmaCI         769  CACGTG               CAC^GTG
PmlI          769  CACGTG               CAC^GTG
PvuII         398  CAGCTG               CAG^CTG
SacI          898  GAGCTC               GAGCT^C
SapI          925  GAAGAGC              GCTCTTC(1/4)
SbfI          503  CCTGCAGG             CCTGCA^GG
SfaNI          65  GCATC                GCATC(5/9)
SpeI          610  ACTAGT               A^CTAGT
SphI         1292  GCATGC               GCATG^C
Sse8387I      503  CCTGCAGG             CCTGCA^GG
StuI         1166  AGGCCT               AGG^CCT
StyI           30  CCATGG               C^C[AT][AT]GG
Van91I        394  CCACCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1327  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         270  CGCG                 CG^CG
             1363  CGCG                
AvaI          308  CTCGGG               C^[CT]CG[GA]G
             1327  CTCGAG              
BbvCI         318  CCTCAGC              CCTCAGC(-5/-2)
              147  GCTGAGG             
BcnI          475  CCGGG                CC^[GC]GG
             1095  CCGGG               
BpmI           24  CTCCAG               CTGGAG(16/14)
              248  CTGGAG              
BsaAI         717  TACGTG               [CT]AC^GT[GA]
              769  CACGTG              
BsaWI         569  ACCGGA               [AT]^CCGG[AT]
              938  ACCGGT              
BsmAI         213  GAGAC                GTCTC(1/5)
              341  GAGAC               
BsmI          240  GAATGC               GAATGC(1/-1)
              583  GAATGC              
BsoBI         308  CTCGGG               C^[CT]CG[GA]G
             1327  CTCGAG              
BsrBI         286  GAGCGG               CCGCTC(-3/-3)
             1013  GAGCGG              
BstAPI        497  GCAGCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              713  GCATTACGTGC         
BstF5I        549  GGATG                GGATG(2/0)
              572  GGATG               
BstUI         270  CGCG                 CG^CG
             1363  CGCG                
BstXI         538  CCATCTTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              945  CCAGCAGTATGG        
DsaI           30  CCATGG               C^C[GA][CT]GG
              217  CCGTGG              
FokI          549  GGATG                GGATG(9/13)
              572  GGATG               
MboII         925  GAAGA                GAAGA(8/7)
               68  TCTTC               
MspA1I        398  CAGCTG               C[AC]G^C[GT]G
             1257  CCGCTG              
MvnI          270  CGCG                 CG^CG
             1363  CGCG                
NciI          475  CCGGG                CC^[GC]GG
             1095  CCGGG               
PstI          504  CTGCAG               CTGCA^G
              973  CTGCAG              
SmlI           76  CTCAAG               C^T[CT][GA]AG
             1327  CTCGAG              
TfiI           52  GATTC                G^A[AT]TC
              915  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       AvaII       
BanII       BbvI        BmyI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      FauI        
Fnu4HI      HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MnlI        MseI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     
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