Restriction map for pF1KB0407
 (Linear) (Six-base) MAPSORT of: KIBB0407.seq  Check: 2885  from: 1  to: 1395



KIBB0407.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     511    1060    1395 
   Size:        511     549     335

  Fragments arranged by size: 

                 549     511     335
 
AatII G_ACGT'C

Cuts at:      0     731    1395 
   Size:        731     664
 
Acc65I G'GTAC_C

Cuts at:      0     941    1395 
   Size:        941     454
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     894    1395 
   Size:        894     501
 
AgeI A'CCGG_T

Cuts at:      0     938    1395 
   Size:        938     457
 
AhdI GACnn_n'nnGTC

Cuts at:      0     533    1395 
   Size:        533     862
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     971    1395 
   Size:        971     424
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1369    1395 
   Size:       1369      26
 
AlwNI CAG_nnn'CTG

Cuts at:      0     503    1395 
   Size:        503     892
 
ApaLI G'TGCA_C

Cuts at:      0     720    1395 
   Size:        720     675
 
ApoI r'AATT_y

Cuts at:      0     619    1395 
   Size:        619     776
 
AvaI C'yCGr_G

Cuts at:      0     308    1327    1395 
   Size:        308    1019      68

  Fragments arranged by size: 

                1019     308      68
 
BanI G'GyrC_C

Cuts at:      0     941    1395 
   Size:        941     454
 
BanII G_rGCy'C

Cuts at:      0     296     739     902    1395 
   Size:        296     443     163     493

  Fragments arranged by size: 

                 493     443     296     163
 
BbvCI CC'TCA_GC

Cuts at:      0     148     319    1395 
   Size:        148     171    1076

  Fragments arranged by size: 

                1076     171     148
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1198    1395 
   Size:       1198     197
 
BlpI GC'TnA_GC

Cuts at:      0      92    1395 
   Size:         92    1303
 
Bme1580I G_kGCm'C

Cuts at:      0     724    1395 
   Size:        724     671
 
BmgBI CAC'GTC

Cuts at:      0    1392    1395 
   Size:       1392       3
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      34    1311    1395 
   Size:         34    1277      84

  Fragments arranged by size: 

                1277      84      34
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     323    1395 
   Size:        323    1072
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       9     269    1395 
   Size:          9     260    1126

  Fragments arranged by size: 

                1126     260       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     148     319    1395 
   Size:        148     171    1076

  Fragments arranged by size: 

                1076     171     148
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61    1395 
   Size:         61    1334
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     207    1395 
   Size:        207    1188
 
BsaAI yAC'GTr

Cuts at:      0     719     771    1395 
   Size:        719      52     624

  Fragments arranged by size: 

                 719     624      52
 
BsaHI Gr'CG_yC

Cuts at:      0     728    1395 
   Size:        728     667
 
BsaWI w'CCGG_w

Cuts at:      0     569     938    1395 
   Size:        569     369     457

  Fragments arranged by size: 

                 569     457     369
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     213     243     332     362    1395 
   Size:        213      30      89      30    1033

  Fragments arranged by size: 

                1033     213      89      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     976    1395 
   Size:        976     419
 
BseYI C'CCAG_C

Cuts at:      0     545    1395 
   Size:        545     850
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     229     352     490     516     635     977    1149 
   Size:        187      42     123     138      26     119     342     172

Cuts at:   1149    1341    1395 
   Size:        192      54

  Fragments arranged by size: 

                 342     192     187     172     138     123     119      54
                  42      26
 
BsiHKAI G_wGCw'C

Cuts at:      0     642     724     902    1395 
   Size:        642      82     178     493

  Fragments arranged by size: 

                 642     493     178      82
 
BsmI GAATG_Cn'

Cuts at:      0     246     589    1395 
   Size:        246     343     806

  Fragments arranged by size: 

                 806     343     246
 
Bsp1286I G_dGCh'C

Cuts at:      0     296     642     724     739     902    1395 
   Size:        296     346      82      15     163     493

  Fragments arranged by size: 

                 493     346     296     163      82      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     403     511    1060    1395 
   Size:        403     108     549     335

  Fragments arranged by size: 

                 549     403     335     108
 
BsrBI CCG'CTC

Cuts at:      0     288    1015    1395 
   Size:        288     727     380

  Fragments arranged by size: 

                 727     380     288
 
BsrDI GCAATG_nn'

Cuts at:      0    1071    1395 
   Size:       1071     324
 
BsrFI r'CCGG_y

Cuts at:      0     938    1395 
   Size:        938     457
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     503     719    1395 
   Size:        503     216     676

  Fragments arranged by size: 

                 676     503     216
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     545     952    1395 
   Size:        545     407     443

  Fragments arranged by size: 

                 545     443     407
 
Bsu36I CC'TnA_GG

Cuts at:      0    1149    1395 
   Size:       1149     246
 
BtgI C'CryG_G

Cuts at:      0      30     217    1395 
   Size:         30     187    1178

  Fragments arranged by size: 

                1178     187      30
 
BtsI GCAGTG_nn'

Cuts at:      0     597    1137    1395 
   Size:        597     540     258

  Fragments arranged by size: 

                 597     540     258
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     757     792    1395 
   Size:        757      35     603

  Fragments arranged by size: 

                 757     603      35
 
DraI TTT'AAA

Cuts at:      0     630    1395 
   Size:        630     765
 
DraIII CAC_nnn'GTG

Cuts at:      0     207    1395 
   Size:        207    1188
 
EarI CTCTTCn'nnn_

Cuts at:      0     920    1395 
   Size:        920     475
 
EcoICRI GAG'CTC

Cuts at:      0     900    1395 
   Size:        900     495
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       9     269     894    1395 
   Size:          9     260     625     501

  Fragments arranged by size: 

                 625     501     260       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     454    1395 
   Size:        454     941
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     213     245     519     551    1395 
   Size:        213      32     274      32     844

  Fragments arranged by size: 

                 844     274     213      32      32
 
KpnI G_GTAC'C

Cuts at:      0     945    1395 
   Size:        945     450
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     679    1395 
   Size:        679     716
 
MslI CAynn'nnrTG

Cuts at:      0    1297    1395 
   Size:       1297      98
 
MspA1I CmG'CkG

Cuts at:      0     400    1259    1395 
   Size:        400     859     136

  Fragments arranged by size: 

                 859     400     136
 
NcoI C'CATG_G

Cuts at:      0      30    1395 
   Size:         30    1365
 
NspI r_CATG'y

Cuts at:      0    1296    1395 
   Size:       1296      99
 
PasI CC'CwG_GG

Cuts at:      0     531     604    1395 
   Size:        531      73     791

  Fragments arranged by size: 

                 791     531      73
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     400    1395 
   Size:        400     995
 
PmlI CAC'GTG

Cuts at:      0     771    1395 
   Size:        771     624
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1146    1178    1395 
   Size:       1146      32     217

  Fragments arranged by size: 

                1146     217      32
 
PspXI vC'TCGA_Gb

Cuts at:      0    1327    1395 
   Size:       1327      68
 
PstI C_TGCA'G

Cuts at:      0     508     977    1395 
   Size:        508     469     418

  Fragments arranged by size: 

                 508     469     418
 
PvuII CAG'CTG

Cuts at:      0     400    1395 
   Size:        400     995
 
SacI G_AGCT'C

Cuts at:      0     902    1395 
   Size:        902     493
 
SapI GCTCTTCn'nnn_

Cuts at:      0     920    1395 
   Size:        920     475
 
SbfI CC_TGCA'GG

Cuts at:      0     508    1395 
   Size:        508     887
 
SfcI C'TryA_G

Cuts at:      0     504     973    1170    1395 
   Size:        504     469     197     225

  Fragments arranged by size: 

                 504     469     225     197
 
SmlI C'TyrA_G

Cuts at:      0      76    1327    1395 
   Size:         76    1251      68

  Fragments arranged by size: 

                1251      76      68
 
SpeI A'CTAG_T

Cuts at:      0     610    1395 
   Size:        610     785
 
SphI G_CATG'C

Cuts at:      0    1296    1395 
   Size:       1296      99
 
StuI AGG'CCT

Cuts at:      0    1168    1395 
   Size:       1168     227
 
StyI C'CwwG_G

Cuts at:      0      30    1395 
   Size:         30    1365
 
TatI w'GTAC_w

Cuts at:      0     932    1395 
   Size:        932     463
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     332     364     564     596    1395 
   Size:        332      32     200      32     799

  Fragments arranged by size: 

                 799     332     200      32      32
 
XhoI C'TCGA_G

Cuts at:      0    1327    1395 
   Size:       1327      68
 
ZraI GAC'GTC

Cuts at:      0     729    1395 
   Size:        729     666
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AgeI     AhdI     AlfI     AloI
    AlwNI    ApaLI     ApoI     AvaI     BanI    BanII    BbvCI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BstAPI
    BstXI   Bsu36I     BtgI     BtsI    CspCI     DraI   DraIII     EarI
  EcoICRI  Eco57MI EcoO109I    Hin4I     KpnI     MmeI     MslI   MspA1I
     NcoI     NspI     PasI    PflMI     PmlI     PpiI    PspXI     PstI
    PvuII     SacI     SapI     SbfI     SfcI     SmlI     SpeI     SphI
     StuI     StyI     TatI     TstI     XhoI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII   AflIII     AleI     ApaI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI    BglII     BmtI    BsaBI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstYI  BstZ17I    BtgZI     ClaI     DrdI     EaeI     EagI     EciI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI     PfoI     PmeI    PpuMI    PshAI     PsiI   PspOMI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI     SspI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XmaI     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com