Restriction map for pF1KB0941
 (Circular) (Six-base) MAPSORT of: pF1KB0941.seq  Check: 3950  from: 1  to: 3620



pF1KB0941  3620 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AccI GT'mk_AC

Cuts at:    581     658     581 
   Size:         77    3543
 
Acc65I G'GTAC_C

Cuts at:    636     636 
   Size:       3620
 
AclI AA'CG_TT

Cuts at:   3460    3460 
   Size:       3620
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1445    1877    2624    2892    1445 
   Size:        432     747     268    2173

  Fragments arranged by size: 

                2173     747     432     268
 
AflIII A'CryG_T

Cuts at:    972    2076    3053     972 
   Size:       1104     977    1539

  Fragments arranged by size: 

                1539    1104     977
 
AgeI A'CCGG_T

Cuts at:   2008    2008 
   Size:       3620
 
AlwNI CAG_nnn'CTG

Cuts at:    925    2492     925 
   Size:       1567    2053
 
ApaLI G'TGCA_C

Cuts at:   2390    2390 
   Size:       3620
 
ApoI r'AATT_y

Cuts at:    624    2973    3229     624 
   Size:       2349     256    1015

  Fragments arranged by size: 

                2349    1015     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3620
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3620
 
AvaI C'yCGr_G

Cuts at:    640     640 
   Size:       3620
 
BamHI G'GATC_C

Cuts at:    645     645 
   Size:       3620
 
BanI G'GyrC_C

Cuts at:    636    1050    1298    1333     636 
   Size:        414     248      35    2923

  Fragments arranged by size: 

                2923     414     248      35
 
BanII G_rGCy'C

Cuts at:    634    1664     634 
   Size:       1030    2590
 
BbeI G_GCGC'C

Cuts at:   1054    1302    1054 
   Size:        248    3372
 
BciVI GTATCCnnnnn_n'

Cuts at:   1518    2279    3620    1518 
   Size:        761    1341    1518

  Fragments arranged by size: 

                1518    1341     761
 
BclI T'GATC_A

Cuts at:   1140    1140 
   Size:       3620
 
BglII A'GATC_T

Cuts at:   1135    3172    1135 
   Size:       2037    1583
 
BlpI GC'TnA_GC

Cuts at:    732     732 
   Size:       3620
 
Bme1580I G_kGCm'C

Cuts at:   1245    1338    2394    1245 
   Size:         93    1056    2471

  Fragments arranged by size: 

                2471    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    832     913    1248    3392     832 
   Size:         81     335    2144    1060

  Fragments arranged by size: 

                2144    1060     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    264    1987     264 
   Size:       1723    1897
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    450     450 
   Size:       3620
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    796    1675    2167    2465     796 
   Size:        879     492     298    1951

  Fragments arranged by size: 

                1951     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    503    3324     503 
   Size:       2821     799
 
BsaAI yAC'GTr

Cuts at:    973     973 
   Size:       3620
 
BsaBI GATnn'nnATC

Cuts at:      6    1134    3171       6 
   Size:       1128    2037     455

  Fragments arranged by size: 

                2037    1128     455
 
BsaHI Gr'CG_yC

Cuts at:   1051    1299    2001    3497    1051 
   Size:        248     702    1496    1174

  Fragments arranged by size: 

                1496    1174     702     248
 
BsaWI w'CCGG_w

Cuts at:    809    1330    2008    2282    2429    2840     809 
   Size:        521     678     274     147     411    1589

  Fragments arranged by size: 

                1589     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1045    1803    2380    2876    1045 
   Size:        758     577     496    1789

  Fragments arranged by size: 

                1789     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1162    1208    2416     104 
   Size:       1058      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1058      46
 
BsiHKAI G_wGCw'C

Cuts at:    634    1412    1602    2394     634 
   Size:        778     190     792    1860

  Fragments arranged by size: 

                1860     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    271     271 
   Size:       3620
 
Bsp1286I G_dGCh'C

Cuts at:    634    1245    1338    1412    1602    1664    2394     634 
   Size:        611      93      74     190      62     730    1860

  Fragments arranged by size: 

                1860     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2840    2840 
   Size:       3620
 
BspHI T'CATG_A

Cuts at:   2796    2796 
   Size:       3620
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    670     884    1186    1567     670 
   Size:        214     302     381    2723

  Fragments arranged by size: 

                2723     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1912    1966    3450    3615      50 
   Size:       1862      54    1484     165      55

  Fragments arranged by size: 

                1862    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1532    1532 
   Size:       3620
 
BsrFI r'CCGG_y

Cuts at:   1618    1799    2008    1618 
   Size:        181     209    3230

  Fragments arranged by size: 

                3230     209     181
 
BssSI C'ACGA_G

Cuts at:   1891    2249    1891 
   Size:        358    3262
 
BstAPI GCAn_nnn'nTGC

Cuts at:    997     997 
   Size:       3620
 
BstBI TT'CG_AA

Cuts at:   1981    1981 
   Size:       3620
 
BstYI r'GATC_y

Cuts at:      1     112     645    1112    1135    1470    1716    2728    2814 
   Size:        111     533     467      23     335     246    1012      86

Cuts at:   2814    2826    3172       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     533     467     449     346     335     246     111
                  86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1570    1751    1914    1570 
   Size:        181     163    3276

  Fragments arranged by size: 

                3276     181     163
 
BtsI GCAGTG_nn'

Cuts at:    604     863     604 
   Size:        259    3361
 
DraI TTT'AAA

Cuts at:    619     619 
   Size:       3620
 
DrdI GACnn_nn'nnGTC

Cuts at:   1326    2184    1326 
   Size:        858    2762
 
EaeI y'GGCC_r

Cuts at:    137    1205    1379    1770    1797    3142     137 
   Size:       1068     174     391      27    1345     615

  Fragments arranged by size: 

                1345    1068     615     391     174      27
 
EagI C'GGCC_G

Cuts at:   1205    1205 
   Size:       3620
 
EarI CTCTTCn'nnn_

Cuts at:    532    1643    1853     532 
   Size:       1111     210    2299

  Fragments arranged by size: 

                2299    1111     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1828    2138    2284    3430    1828 
   Size:        310     146    1146    2018

  Fragments arranged by size: 

                2018    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    632     632 
   Size:       3620
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    450    1445    1877    2624    2892     450 
   Size:        995     432     747     268    1178

  Fragments arranged by size: 

                1178     995     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    374     374 
   Size:       3620
 
EcoO109I rG'GnC_Cy

Cuts at:    759     759 
   Size:       3620
 
EcoRI G'AATT_C

Cuts at:    624     624 
   Size:       3620
 
EcoRV GAT'ATC

Cuts at:    327     327 
   Size:       3620
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1639    1639 
   Size:       3620
 
FspI TGC'GCA

Cuts at:   1401    1401 
   Size:       3620
 
HaeII r_GCGC'y

Cuts at:   1054    1302    2324    3303    1054 
   Size:        248    1022     979    1371

  Fragments arranged by size: 

                1371    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    433     465    1462    1492    1494    1524     433 
   Size:         32     997      30       2      30    2529

  Fragments arranged by size: 

                2529     997      32      30      30       2
 
HincII GTy'rAC

Cuts at:    659     659 
   Size:       3620
 
KasI G'GCGC_C

Cuts at:   1050    1298    1050 
   Size:        248    3372
 
KpnI G_GTAC'C

Cuts at:    640     640 
   Size:       3620
 
MluI A'CGCG_T

Cuts at:   3053    3053 
   Size:       3620
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2291    2475    2291 
   Size:        184    3436
 
MscI TGG'CCA

Cuts at:    139    1381     139 
   Size:       1242    2378
 
MslI CAynn'nnrTG

Cuts at:   1609    1736    1609 
   Size:        127    3493
 
MspA1I CmG'CkG

Cuts at:    731    1045    1405    2418    2663     731 
   Size:        314     360    1013     245    1688

  Fragments arranged by size: 

                1688    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1801    1801 
   Size:       3620
 
NarI GG'CG_CC

Cuts at:   1051    1299    1051 
   Size:        248    3372
 
NgoMIV G'CCGG_C

Cuts at:   1799    1799 
   Size:       3620
 
NspI r_CATG'y

Cuts at:    588     673    1173    2080     588 
   Size:         85     500     907    2128

  Fragments arranged by size: 

                2128     907     500      85
 
PciI A'CATG_T

Cuts at:   2076    2076 
   Size:       3620
 
PmeI GTTT'AAAC

Cuts at:    619     619 
   Size:       3620
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2759    2791    2759 
   Size:         32    3588
 
PstI C_TGCA'G

Cuts at:    599     667    1352     599 
   Size:         68     685    2867

  Fragments arranged by size: 

                2867     685      68
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3620
 
PvuII CAG'CTG

Cuts at:   1045    1405    1045 
   Size:        360    3260
 
RsrII CG'GwC_CG

Cuts at:   1815    1815 
   Size:       3620
 
SacI G_AGCT'C

Cuts at:    634     634 
   Size:       3620
 
SalI G'TCGA_C

Cuts at:    657     657 
   Size:       3620
 
SapI GCTCTTCn'nnn_

Cuts at:   1643    1853    1643 
   Size:        210    3410
 
SbfI CC_TGCA'GG

Cuts at:    667     667 
   Size:       3620
 
ScaI AGT'ACT

Cuts at:   3137    3137 
   Size:       3620
 
SfcI C'TryA_G

Cuts at:     33     595     663    1348    2341    2532      33 
   Size:        562      68     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     562     191      68
 
SfoI GGC'GCC

Cuts at:   1052    1300    1052 
   Size:        248    3372
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3620
 
SmaI CCC'GGG

Cuts at:    642     642 
   Size:       3620
 
SmlI C'TyrA_G

Cuts at:    775    1690    2182    2444     775 
   Size:        915     492     262    1951

  Fragments arranged by size: 

                1951     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3620
 
SphI G_CATG'C

Cuts at:    673    1173     673 
   Size:        500    3120
 
SspI AAT'ATT

Cuts at:    234     234 
   Size:       3620
 
StyI C'CwwG_G

Cuts at:    493     754    1108     493 
   Size:        261     354    3005

  Fragments arranged by size: 

                3005     354     261
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1203    1336    1795    2004    1203 
   Size:        133     459     209    2819

  Fragments arranged by size: 

                2819     459     209     133
 
TatI w'GTAC_w

Cuts at:   3135    3135 
   Size:       3620
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3137    3169    3137 
   Size:         32    3588
 
Tth111I GACn'n_nGTC

Cuts at:   1417    1417 
   Size:       3620
 
XbaI T'CTAG_A

Cuts at:    651     651 
   Size:       3620
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1115    1115 
   Size:       3620
 
XmaI C'CCGG_G

Cuts at:    640     640 
   Size:       3620
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PciI     PmeI     PpiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
     ClaI    CspCI   DraIII     FseI    FspAI  HindIII     HpaI     MfeI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     StuI     SwaI     XhoI     XmnI     ZraI
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