Restriction map for
pF1KB0940
(Circular) (Six-base) MAPSORT of: pF1KB0940.seq Check: 9782 from: 1 to: 4283
pF1KB0940 4283 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AatII G_ACGT'C
Cuts at: 440 440
Size: 4283
AccI GT'mk_AC
Cuts at: 1321 1321
Size: 4283
Acc65I G'GTAC_C
Cuts at: 1299 1299
Size: 4283
AclI AA'CG_TT
Cuts at: 4123 4123
Size: 4283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1031 2108 2540 3287 3555 1031
Size: 1077 432 747 268 1759
Fragments arranged by size:
1759 1077 747 432 268
AflIII A'CryG_T
Cuts at: 815 1635 2739 3716 815
Size: 820 1104 977 1382
Fragments arranged by size:
1382 1104 977 820
AgeI A'CCGG_T
Cuts at: 620 2671 620
Size: 2051 2232
AlwNI CAG_nnn'CTG
Cuts at: 1588 3155 1588
Size: 1567 2716
ApaLI G'TGCA_C
Cuts at: 3053 3053
Size: 4283
ApoI r'AATT_y
Cuts at: 1287 3636 3892 1287
Size: 2349 256 1678
Fragments arranged by size:
2349 1678 256
AseI AT'TA_AT
Cuts at: 20 1079 20
Size: 1059 3224
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
AvaI C'yCGr_G
Cuts at: 1303 1303
Size: 4283
BamHI G'GATC_C
Cuts at: 1308 1308
Size: 4283
BanI G'GyrC_C
Cuts at: 1299 1713 1961 1996 1299
Size: 414 248 35 3586
Fragments arranged by size:
3586 414 248 35
BanII G_rGCy'C
Cuts at: 301 1297 2327 301
Size: 996 1030 2257
Fragments arranged by size:
2257 1030 996
BbeI G_GCGC'C
Cuts at: 1717 1965 1717
Size: 248 4035
BciVI GTATCCnnnnn_n'
Cuts at: 2181 2942 4283 2181
Size: 761 1341 2181
Fragments arranged by size:
2181 1341 761
BclI T'GATC_A
Cuts at: 1803 1803
Size: 4283
BglII A'GATC_T
Cuts at: 1798 3835 1798
Size: 2037 2246
BlpI GC'TnA_GC
Cuts at: 1395 1395
Size: 4283
Bme1580I G_kGCm'C
Cuts at: 1908 2001 3057 1908
Size: 93 1056 3134
Fragments arranged by size:
3134 1056 93
BmgBI CAC'GTC
Cuts at: 818 818
Size: 4283
BmrI ACTGGGnnnn_n'
Cuts at: 1495 1576 1911 4055 1495
Size: 81 335 2144 1723
Fragments arranged by size:
2144 1723 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 4283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1459 2338 2830 3128 1459
Size: 879 492 298 2614
Fragments arranged by size:
2614 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3987 3987
Size: 4283
BsaAI yAC'GTr
Cuts at: 1636 1636
Size: 4283
BsaBI GATnn'nnATC
Cuts at: 6 690 1797 3834 6
Size: 684 1107 2037 455
Fragments arranged by size:
2037 1107 684 455
BsaHI Gr'CG_yC
Cuts at: 437 1714 1962 2664 4160 437
Size: 1277 248 702 1496 560
Fragments arranged by size:
1496 1277 702 560 248
BsaWI w'CCGG_w
Cuts at: 620 1472 1993 2671 2945 3092 3503 620
Size: 852 521 678 274 147 411 1400
Fragments arranged by size:
1400 852 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1166 1196 1249 1279 1166
Size: 30 53 30 4170
Fragments arranged by size:
4170 53 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 227 291 227
Size: 64 4219
BseYI C'CCAG_C
Cuts at: 289 880 1708 2466 3043 3539 289
Size: 591 828 758 577 496 1033
Fragments arranged by size:
1033 828 758 591 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 1120 226
Size: 894 3389
BsiEI CG_ry'CG
Cuts at: 104 1825 1871 3079 104
Size: 1721 46 1208 1308
Fragments arranged by size:
1721 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1218 1297 2075 2265 3057 1218
Size: 79 778 190 792 2444
Fragments arranged by size:
2444 792 778 190 79
Bsp1286I G_dGCh'C
Cuts at: 301 1218 1297 1908 2001 2075 2265 2327 3057
Size: 917 79 611 93 74 190 62 730
Cuts at: 3057 301
Size: 1527
Fragments arranged by size:
1527 917 730 611 190 93 79 74
62
BspEI T'CCGG_A
Cuts at: 3503 3503
Size: 4283
BspHI T'CATG_A
Cuts at: 756 965 3459 756
Size: 209 2494 1580
Fragments arranged by size:
2494 1580 209
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1333 1547 1849 2230 1333
Size: 214 302 381 3386
Fragments arranged by size:
3386 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2575 2629 4113 4278 50
Size: 2525 54 1484 165 55
Fragments arranged by size:
2525 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2195 2195
Size: 4283
BsrFI r'CCGG_y
Cuts at: 210 620 2281 2462 2671 210
Size: 410 1661 181 209 1822
Fragments arranged by size:
1822 1661 410 209 181
BssSI C'ACGA_G
Cuts at: 2554 2912 2554
Size: 358 3925
BstAPI GCAn_nnn'nTGC
Cuts at: 1660 1660
Size: 4283
BstBI TT'CG_AA
Cuts at: 2644 2644
Size: 4283
BstXI CCAn_nnnn'nTGG
Cuts at: 282 282
Size: 4283
BstYI r'GATC_y
Cuts at: 1 1308 1775 1798 2133 2379 3391 3477 3489
Size: 1307 467 23 335 246 1012 86 12
Cuts at: 3489 3835 1
Size: 346 449
Fragments arranged by size:
1307 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 228 281 1169 107
Size: 121 53 888 3221
Fragments arranged by size:
3221 888 121 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2233 2414 2577 2233
Size: 181 163 3939
Fragments arranged by size:
3939 181 163
BtsI GCAGTG_nn'
Cuts at: 846 1270 1526 846
Size: 424 256 3603
Fragments arranged by size:
3603 424 256
ClaI AT'CG_AT
Cuts at: 656 656
Size: 4283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 915 950 915
Size: 35 4248
DraI TTT'AAA
Cuts at: 320 1282 320
Size: 962 3321
DraIII CAC_nnn'GTG
Cuts at: 826 826
Size: 4283
DrdI GACnn_nn'nnGTC
Cuts at: 1989 2847 1989
Size: 858 3425
EaeI y'GGCC_r
Cuts at: 810 1868 2042 2433 2460 3805 810
Size: 1058 174 391 27 1345 1288
Fragments arranged by size:
1345 1288 1058 391 174 27
EagI C'GGCC_G
Cuts at: 1868 1868
Size: 4283
EarI CTCTTCn'nnn_
Cuts at: 361 2306 2516 361
Size: 1945 210 2128
Fragments arranged by size:
2128 1945 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2491 2801 2947 4093 2491
Size: 310 146 1146 2681
Fragments arranged by size:
2681 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1295 1295
Size: 4283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1031 2108 2540 3287 3555 1031
Size: 1077 432 747 268 1759
Fragments arranged by size:
1759 1077 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 183 796 1222 1422 183
Size: 613 426 200 3044
Fragments arranged by size:
3044 613 426 200
EcoRI G'AATT_C
Cuts at: 1287 1287
Size: 4283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 4283
FspI TGC'GCA
Cuts at: 2064 2064
Size: 4283
HaeII r_GCGC'y
Cuts at: 1717 1965 2987 3966 1717
Size: 248 1022 979 2034
Fragments arranged by size:
2034 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 146 178 338 370 427 459 724 754 756
Size: 32 160 32 57 32 265 30 2
Cuts at: 756 786 1037 1069 2125 2155 2157 2187 146
Size: 30 251 32 1056 30 2 30 2242
Fragments arranged by size:
2242 1056 265 251 160 57 32 32
32 32 30 30 30 30 2 2
HincII GTy'rAC
Cuts at: 1322 1322
Size: 4283
KasI G'GCGC_C
Cuts at: 1713 1961 1713
Size: 248 4035
KpnI G_GTAC'C
Cuts at: 1303 1303
Size: 4283
MfeI C'AATT_G
Cuts at: 1068 1068
Size: 4283
MluI A'CGCG_T
Cuts at: 3716 3716
Size: 4283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1145 2954 3138 1145
Size: 1809 184 2290
Fragments arranged by size:
2290 1809 184
MscI TGG'CCA
Cuts at: 812 2044 812
Size: 1232 3051
MslI CAynn'nnrTG
Cuts at: 1246 2272 2399 1246
Size: 1026 127 3130
Fragments arranged by size:
3130 1026 127
MspA1I CmG'CkG
Cuts at: 806 1394 1708 2068 3081 3326 806
Size: 588 314 360 1013 245 1763
Fragments arranged by size:
1763 1013 588 360 314 245
NaeI GCC'GGC
Cuts at: 2464 2464
Size: 4283
NarI GG'CG_CC
Cuts at: 1714 1962 1714
Size: 248 4035
NcoI C'CATG_G
Cuts at: 107 281 1169 107
Size: 174 888 3221
Fragments arranged by size:
3221 888 174
NdeI CA'TA_TG
Cuts at: 1202 1202
Size: 4283
NgoMIV G'CCGG_C
Cuts at: 2462 2462
Size: 4283
NspI r_CATG'y
Cuts at: 193 1336 1836 2743 193
Size: 1143 500 907 1733
Fragments arranged by size:
1733 1143 907 500
PciI A'CATG_T
Cuts at: 2739 2739
Size: 4283
PmeI GTTT'AAAC
Cuts at: 1282 1282
Size: 4283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 4251
PpuMI rG'GwC_Cy
Cuts at: 183 1222 183
Size: 1039 3244
PsiI TTA'TAA
Cuts at: 1151 1151
Size: 4283
PstI C_TGCA'G
Cuts at: 1330 2015 1330
Size: 685 3598
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4283
PvuII CAG'CTG
Cuts at: 1708 2068 1708
Size: 360 3923
RsrII CG'GwC_CG
Cuts at: 2478 2478
Size: 4283
SacI G_AGCT'C
Cuts at: 1297 1297
Size: 4283
SalI G'TCGA_C
Cuts at: 1320 1320
Size: 4283
SanDI GG'GwC_CC
Cuts at: 183 183
Size: 4283
SapI GCTCTTCn'nnn_
Cuts at: 2306 2516 2306
Size: 210 4073
SbfI CC_TGCA'GG
Cuts at: 1330 1330
Size: 4283
ScaI AGT'ACT
Cuts at: 3800 3800
Size: 4283
SfcI C'TryA_G
Cuts at: 33 1326 2011 3004 3195 33
Size: 1293 685 993 191 1121
Fragments arranged by size:
1293 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1715 1963 1715
Size: 248 4035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
SmaI CCC'GGG
Cuts at: 1305 1305
Size: 4283
SmlI C'TyrA_G
Cuts at: 1438 2353 2845 3107 1438
Size: 915 492 262 2614
Fragments arranged by size:
2614 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4283
SphI G_CATG'C
Cuts at: 193 1336 1836 193
Size: 1143 500 2640
Fragments arranged by size:
2640 1143 500
StyI C'CwwG_G
Cuts at: 107 281 1169 1417 1771 107
Size: 174 888 248 354 2619
Fragments arranged by size:
2619 888 354 248 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1866 1999 2458 2667 1866
Size: 133 459 209 3482
Fragments arranged by size:
3482 459 209 133
TatI w'GTAC_w
Cuts at: 3798 3798
Size: 4283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1249 1281 3800 3832 1249
Size: 32 2519 32 1700
Fragments arranged by size:
2519 1700 32 32
Tth111I GACn'n_nGTC
Cuts at: 2080 2080
Size: 4283
XbaI T'CTAG_A
Cuts at: 1314 1314
Size: 4283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1778 1778
Size: 4283
XmaI C'CCGG_G
Cuts at: 1303 1303
Size: 4283
ZraI GAC'GTC
Cuts at: 438 438
Size: 4283
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I EcoNI EcoRV FseI FspAI HindIII
HpaI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI