Restriction map for pF1KB0940
 (Linear) (Six-base) MAPSORT of: KIBB0940.seq  Check: 6621  from: 1  to: 1170



KIBB0940.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AatII G_ACGT'C

Cuts at:      0     332    1170 
   Size:        332     838
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     923    1170 
   Size:        923     247
 
AflIII A'CryG_T

Cuts at:      0     707    1170 
   Size:        707     463
 
AgeI A'CCGG_T

Cuts at:      0     512    1170 
   Size:        512     658
 
AseI AT'TA_AT

Cuts at:      0     971    1170 
   Size:        971     199
 
BanII G_rGCy'C

Cuts at:      0     193    1170 
   Size:        193     977
 
BmgBI CAC'GTC

Cuts at:      0     710    1170 
   Size:        710     460
 
BsaBI GATnn'nnATC

Cuts at:      0     582    1170 
   Size:        582     588
 
BsaHI Gr'CG_yC

Cuts at:      0     329    1170 
   Size:        329     841
 
BsaWI w'CCGG_w

Cuts at:      0     512    1170 
   Size:        512     658
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1058    1088    1141    1170 
   Size:       1058      30      53      29

  Fragments arranged by size: 

                1058      53      30      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     119     183    1170 
   Size:        119      64     987

  Fragments arranged by size: 

                 987     119      64
 
BseYI C'CCAG_C

Cuts at:      0     181     772    1170 
   Size:        181     591     398

  Fragments arranged by size: 

                 591     398     181
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118    1012    1170 
   Size:        118     894     158

  Fragments arranged by size: 

                 894     158     118
 
BsiHKAI G_wGCw'C

Cuts at:      0    1110    1170 
   Size:       1110      60
 
Bsp1286I G_dGCh'C

Cuts at:      0     193    1110    1170 
   Size:        193     917      60

  Fragments arranged by size: 

                 917     193      60
 
BspHI T'CATG_A

Cuts at:      0     648     857    1170 
   Size:        648     209     313

  Fragments arranged by size: 

                 648     313     209
 
BsrFI r'CCGG_y

Cuts at:      0     102     512    1170 
   Size:        102     410     658

  Fragments arranged by size: 

                 658     410     102
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     174    1170 
   Size:        174     996
 
BtgI C'CryG_G

Cuts at:      0     120     173    1061    1170 
   Size:        120      53     888     109

  Fragments arranged by size: 

                 888     120     109      53
 
BtsI GCAGTG_nn'

Cuts at:      0     738    1162    1170 
   Size:        738     424       8

  Fragments arranged by size: 

                 738     424       8
 
ClaI AT'CG_AT

Cuts at:      0     548    1170 
   Size:        548     622
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     807     842    1170 
   Size:        807      35     328

  Fragments arranged by size: 

                 807     328      35
 
DraI TTT'AAA

Cuts at:      0     212    1170 
   Size:        212     958
 
DraIII CAC_nnn'GTG

Cuts at:      0     718    1170 
   Size:        718     452
 
EaeI y'GGCC_r

Cuts at:      0     702    1170 
   Size:        702     468
 
EarI CTCTTCn'nnn_

Cuts at:      0     253    1170 
   Size:        253     917
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     923    1170 
   Size:        923     247
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75     688    1114    1170 
   Size:         75     613     426      56

  Fragments arranged by size: 

                 613     426      75      56
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      38      70     230     262     319     351     616     646 
   Size:         38      32     160      32      57      32     265      30

Cuts at:    646     648     678     929     961    1170 
   Size:          2      30     251      32     209

  Fragments arranged by size: 

                 265     251     209     160      57      38      32      32
                  32      32      30      30       2
 
MfeI C'AATT_G

Cuts at:      0     960    1170 
   Size:        960     210
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1037    1170 
   Size:       1037     133
 
MscI TGG'CCA

Cuts at:      0     704    1170 
   Size:        704     466
 
MslI CAynn'nnrTG

Cuts at:      0    1138    1170 
   Size:       1138      32
 
MspA1I CmG'CkG

Cuts at:      0     698    1170 
   Size:        698     472
 
NcoI C'CATG_G

Cuts at:      0     173    1061    1170 
   Size:        173     888     109

  Fragments arranged by size: 

                 888     173     109
 
NdeI CA'TA_TG

Cuts at:      0    1094    1170 
   Size:       1094      76
 
NspI r_CATG'y

Cuts at:      0      85    1170 
   Size:         85    1085
 
PpuMI rG'GwC_Cy

Cuts at:      0      75    1114    1170 
   Size:         75    1039      56

  Fragments arranged by size: 

                1039      75      56
 
PsiI TTA'TAA

Cuts at:      0    1043    1170 
   Size:       1043     127
 
SanDI GG'GwC_CC

Cuts at:      0      75    1170 
   Size:         75    1095
 
SphI G_CATG'C

Cuts at:      0      85    1170 
   Size:         85    1085
 
StyI C'CwwG_G

Cuts at:      0     173    1061    1170 
   Size:        173     888     109

  Fragments arranged by size: 

                 888     173     109
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1141    1170 
   Size:       1141      29
 
ZraI GAC'GTC

Cuts at:      0     330    1170 
   Size:        330     840
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AgeI     AseI    BanII    BmgBI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspHI    BsrFI    BstXI     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     EaeI     EarI  Eco57MI EcoO109I    Hin4I     MfeI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NspI    PpuMI     PsiI
    SanDI     SphI     StyI     TstI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I    BtgZI     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MluI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII
     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
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