Restriction map for pF1KB0926
 (Circular) (Six-base) MAPSORT of: pF1KB0926.seq  Check: 8722  from: 1  to: 4181



pF1KB0926  4181 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    458     458 
   Size:       4181
 
AccI GT'mk_AC

Cuts at:   1219    1219 
   Size:       4181
 
Acc65I G'GTAC_C

Cuts at:   1197    1197 
   Size:       4181
 
AclI AA'CG_TT

Cuts at:   4021    4021 
   Size:       4181
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    522    1026    1102    1135    1180    2006    2438    3185    3453 
   Size:        504      76      33      45     826     432     747     268

Cuts at:   3453     522 
   Size:       1250

  Fragments arranged by size: 

                1250     826     747     504     432     268      76      45
                  33
 
AflIII A'CryG_T

Cuts at:   1533    2637    3614    1533 
   Size:       1104     977    2100

  Fragments arranged by size: 

                2100    1104     977
 
AgeI A'CCGG_T

Cuts at:   2569    2569 
   Size:       4181
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    263    1087     263 
   Size:        824    3357
 
AlwNI CAG_nnn'CTG

Cuts at:    372     450     563    1020    1486    3053     372 
   Size:         78     113     457     466    1567    1500

  Fragments arranged by size: 

                1567    1500     466     457     113      78
 
ApaI G_GGCC'C

Cuts at:    257     305     257 
   Size:         48    4133
 
ApaLI G'TGCA_C

Cuts at:   2951    2951 
   Size:       4181
 
ApoI r'AATT_y

Cuts at:   1185    3534    3790    1185 
   Size:       2349     256    1576

  Fragments arranged by size: 

                2349    1576     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4181
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4181
 
AvaI C'yCGr_G

Cuts at:    304    1201     304 
   Size:        897    3284
 
BamHI G'GATC_C

Cuts at:   1206    1206 
   Size:       4181
 
BanI G'GyrC_C

Cuts at:    837    1197    1611    1859    1894     837 
   Size:        360     414     248      35    3124

  Fragments arranged by size: 

                3124     414     360     248      35
 
BanII G_rGCy'C

Cuts at:    152     257     305     712    1040    1195    2225     152 
   Size:        105      48     407     328     155    1030    2108

  Fragments arranged by size: 

                2108    1030     407     328     155     105      48
 
BbeI G_GCGC'C

Cuts at:   1615    1863    1615 
   Size:        248    3933
 
BbsI GAAGACnn'nnnn_

Cuts at:    369     812     369 
   Size:        443    3738
 
BbvCI CC'TCA_GC

Cuts at:    157     847     157 
   Size:        690    3491
 
BciVI GTATCCnnnnn_n'

Cuts at:   2079    2840    4181    2079 
   Size:        761    1341    2079

  Fragments arranged by size: 

                2079    1341     761
 
BclI T'GATC_A

Cuts at:   1701    1701 
   Size:       4181
 
BglI GCCn_nnn'nGGC

Cuts at:    208     309     208 
   Size:        101    4080
 
BglII A'GATC_T

Cuts at:   1696    3733    1696 
   Size:       2037    2144
 
BlpI GC'TnA_GC

Cuts at:   1293    1293 
   Size:       4181
 
Bme1580I G_kGCm'C

Cuts at:    257     305    1806    1899    2955     257 
   Size:         48    1501      93    1056    1483

  Fragments arranged by size: 

                1501    1483    1056      93      48
 
BmgBI CAC'GTC

Cuts at:    984     984 
   Size:       4181
 
BmrI ACTGGGnnnn_n'

Cuts at:   1393    1474    1809    3953    1393 
   Size:         81     335    2144    1621

  Fragments arranged by size: 

                2144    1621     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2548    2548 
   Size:       4181
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    283     776     839     846     283 
   Size:        493      63       7    3618

  Fragments arranged by size: 

                3618     493      63       7
 
Bpu10I CC'TnA_GC

Cuts at:    117     157     847     117 
   Size:         40     690    3451

  Fragments arranged by size: 

                3451     690      40
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    894    1357    2236    2728    3026     894 
   Size:        463     879     492     298    2049

  Fragments arranged by size: 

                2049     879     492     463     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3885    3885 
   Size:       4181
 
BsaAI yAC'GTr

Cuts at:   1032    1534    1032 
   Size:        502    3679
 
BsaBI GATnn'nnATC

Cuts at:      6    1695    3732       6 
   Size:       1689    2037     455

  Fragments arranged by size: 

                2037    1689     455
 
BsaHI Gr'CG_yC

Cuts at:   1004    1612    1860    2562    4058    1004 
   Size:        608     248     702    1496    1127

  Fragments arranged by size: 

                1496    1127     702     608     248
 
BsaWI w'CCGG_w

Cuts at:   1370    1891    2569    2843    2990    3401    1370 
   Size:        521     678     274     147     411    2150

  Fragments arranged by size: 

                2150     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    145     175     694     709     724     739     145 
   Size:         30     519      15      15      15    3587

  Fragments arranged by size: 

                3587     519      30      15      15      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    714     717     714 
   Size:          3    4178
 
BseYI C'CCAG_C

Cuts at:    190    1119    1606    2364    2941    3437     190 
   Size:        929     487     758     577     496     934

  Fragments arranged by size: 

                 934     929     758     577     496     487
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    298     298 
   Size:       4181
 
BsiEI CG_ry'CG

Cuts at:    104    1723    1769    2977     104 
   Size:       1619      46    1208    1308

  Fragments arranged by size: 

                1619    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1040    1195    1973    2163    2955    1040 
   Size:        155     778     190     792    2266

  Fragments arranged by size: 

                2266     792     778     190     155
 
Bsp1286I G_dGCh'C

Cuts at:    152     257     305     712    1040    1195    1806    1899    1973 
   Size:        105      48     407     328     155     611      93      74

Cuts at:   1973    2163    2225    2955     152 
   Size:        190      62     730    1378

  Fragments arranged by size: 

                1378     730     611     407     328     190     155     105
                  93      74      62      48
 
BspEI T'CCGG_A

Cuts at:   3401    3401 
   Size:       4181
 
BspHI T'CATG_A

Cuts at:   1097    3357    1097 
   Size:       2260    1921
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    458    1231    1445    1747    2128     458 
   Size:        773     214     302     381    2511

  Fragments arranged by size: 

                2511     773     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     309    2473    2527    4011    4176      50 
   Size:        259    2164      54    1484     165      55

  Fragments arranged by size: 

                2164    1484     259     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2093    2093 
   Size:       4181
 
BsrFI r'CCGG_y

Cuts at:   2179    2360    2569    2179 
   Size:        181     209    3791

  Fragments arranged by size: 

                3791     209     181
 
BssSI C'ACGA_G

Cuts at:   1051    2452    2810    1051 
   Size:       1401     358    2422

  Fragments arranged by size: 

                2422    1401     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    353    1558     353 
   Size:       1205    2976
 
BstBI TT'CG_AA

Cuts at:   2542    2542 
   Size:       4181
 
BstYI r'GATC_y

Cuts at:      1    1206    1673    1696    2031    2277    3289    3375    3387 
   Size:       1205     467      23     335     246    1012      86      12

Cuts at:   3387    3733       1 
   Size:        346     449

  Fragments arranged by size: 

                1205    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    170     170 
   Size:       4181
 
BtgI C'CryG_G

Cuts at:    133     182     946     133 
   Size:         49     764    3368

  Fragments arranged by size: 

                3368     764      49
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    542    2131    2312    2475     542 
   Size:       1589     181     163    2248

  Fragments arranged by size: 

                2248    1589     181     163
 
BtsI GCAGTG_nn'

Cuts at:    573    1424     573 
   Size:        851    3330
 
DraI TTT'AAA

Cuts at:   1180    1180 
   Size:       4181
 
DrdI GACnn_nn'nnGTC

Cuts at:   1887    2745    1887 
   Size:        858    3323
 
EaeI y'GGCC_r

Cuts at:    310     943    1766    1940    2331    2358    3703     310 
   Size:        633     823     174     391      27    1345     788

  Fragments arranged by size: 

                1345     823     788     633     391     174      27
 
EagI C'GGCC_G

Cuts at:   1766    1766 
   Size:       4181
 
EarI CTCTTCn'nnn_

Cuts at:    541     629    1045    1078    2204    2414     541 
   Size:         88     416      33    1126     210    2308

  Fragments arranged by size: 

                2308    1126     416     210      88      33
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    675     954    2389    2699    2845    3991     675 
   Size:        279    1435     310     146    1146     865

  Fragments arranged by size: 

                1435    1146     865     310     279     146
 
EcoICRI GAG'CTC

Cuts at:   1038    1193    1038 
   Size:        155    4026
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    283     522     776     839     846    1026    1102    1135    1180 
   Size:        239     254      63       7     180      76      33      45

Cuts at:   1180    2006    2438    3185    3453     283 
   Size:        826     432     747     268    1011

  Fragments arranged by size: 

                1011     826     747     432     268     254     239     180
                  76      63      45      33       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:    555     555 
   Size:       4181
 
EcoO109I rG'GnC_Cy

Cuts at:    253     254    1024    1123    1320     253 
   Size:          1     770      99     197    3114

  Fragments arranged by size: 

                3114     770     197      99       1
 
EcoRI G'AATT_C

Cuts at:   1185    1185 
   Size:       4181
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    393    2200     393 
   Size:       1807    2374
 
FspI TGC'GCA

Cuts at:    929    1962     929 
   Size:       1033    3148
 
HaeII r_GCGC'y

Cuts at:   1615    1863    2885    3864    1615 
   Size:        248    1022     979    1932

  Fragments arranged by size: 

                1932    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    145     177     298     330     400     432    2023    2053    2055 
   Size:         32     121      32      70      32    1591      30       2

Cuts at:   2055    2085     145 
   Size:         30    2241

  Fragments arranged by size: 

                2241    1591     121      70      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1220    1220 
   Size:       4181
 
KasI G'GCGC_C

Cuts at:   1611    1859    1611 
   Size:        248    3933
 
KpnI G_GTAC'C

Cuts at:   1201    1201 
   Size:       4181
 
MluI A'CGCG_T

Cuts at:   3614    3614 
   Size:       4181
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2852    3036    2852 
   Size:        184    3997
 
MscI TGG'CCA

Cuts at:   1942    1942 
   Size:       4181
 
MslI CAynn'nnrTG

Cuts at:   1096    2170    2297    1096 
   Size:       1074     127    2980

  Fragments arranged by size: 

                2980    1074     127
 
MspA1I CmG'CkG

Cuts at:    135     793    1292    1606    1966    2979    3224     135 
   Size:        658     499     314     360    1013     245    1092

  Fragments arranged by size: 

                1092    1013     658     499     360     314     245
 
NaeI GCC'GGC

Cuts at:   2362    2362 
   Size:       4181
 
NarI GG'CG_CC

Cuts at:   1612    1860    1612 
   Size:        248    3933
 
NgoMIV G'CCGG_C

Cuts at:   2360    2360 
   Size:       4181
 
NspI r_CATG'y

Cuts at:   1234    1734    2641    1234 
   Size:        500     907    2774

  Fragments arranged by size: 

                2774     907     500
 
PasI CC'CwG_GG

Cuts at:    557     557 
   Size:       4181
 
PciI A'CATG_T

Cuts at:   2637    2637 
   Size:       4181
 
PfoI T'CCnGG_A

Cuts at:    287     584     287 
   Size:        297    3884
 
PmeI GTTT'AAAC

Cuts at:   1180    1180 
   Size:       4181
 
PmlI CAC'GTG

Cuts at:   1032    1032 
   Size:       4181
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3320    3352    3320 
   Size:         32    4149
 
PpuMI rG'GwC_Cy

Cuts at:   1123    1123 
   Size:       4181
 
PspOMI G'GGCC_C

Cuts at:    253     301     253 
   Size:         48    4133
 
PstI C_TGCA'G

Cuts at:    329     568    1228    1913     329 
   Size:        239     660     685    2597

  Fragments arranged by size: 

                2597     685     660     239
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4181
 
PvuII CAG'CTG

Cuts at:   1606    1966    1606 
   Size:        360    3821
 
RsrII CG'GwC_CG

Cuts at:   2376    2376 
   Size:       4181
 
SacI G_AGCT'C

Cuts at:   1040    1195    1040 
   Size:        155    4026
 
SacII CC_GC'GG

Cuts at:    136     136 
   Size:       4181
 
SalI G'TCGA_C

Cuts at:   1218    1218 
   Size:       4181
 
SanDI GG'GwC_CC

Cuts at:   1123    1123 
   Size:       4181
 
SapI GCTCTTCn'nnn_

Cuts at:   1045    2204    2414    1045 
   Size:       1159     210    2812

  Fragments arranged by size: 

                2812    1159     210
 
SbfI CC_TGCA'GG

Cuts at:   1228    1228 
   Size:       4181
 
ScaI AGT'ACT

Cuts at:   3698    3698 
   Size:       4181
 
SexAI A'CCwGG_T

Cuts at:    641     641 
   Size:       4181
 
SfcI C'TryA_G

Cuts at:     33     325     564     669    1224    1909    2902    3093      33 
   Size:        292     239     105     555     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     555     292     239     191     105
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    309     309 
   Size:       4181
 
SfoI GGC'GCC

Cuts at:   1613    1861    1613 
   Size:        248    3933
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4181
 
SmaI CCC'GGG

Cuts at:   1203    1203 
   Size:       4181
 
SmlI C'TyrA_G

Cuts at:    909    1336    2251    2743    3005     909 
   Size:        427     915     492     262    2085

  Fragments arranged by size: 

                2085     915     492     427     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4181
 
SphI G_CATG'C

Cuts at:   1234    1734    1234 
   Size:        500    3681
 
StuI AGG'CCT

Cuts at:    852     852 
   Size:       4181
 
StyI C'CwwG_G

Cuts at:    768    1315    1669     768 
   Size:        547     354    3280

  Fragments arranged by size: 

                3280     547     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1764    1897    2356    2565    1764 
   Size:        133     459     209    3380

  Fragments arranged by size: 

                3380     459     209     133
 
TatI w'GTAC_w

Cuts at:    734    3696     734 
   Size:       2962    1219
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    694     709     726     741     973    1005    3698    3730     694 
   Size:         15      17      15     232      32    2693      32    1145

  Fragments arranged by size: 

                2693    1145     232      32      32      17      15      15
            
 
Tth111I GACn'n_nGTC

Cuts at:   1093    1978    1093 
   Size:        885    3296
 
XbaI T'CTAG_A

Cuts at:   1212    1212 
   Size:       4181
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    772    1676     772 
   Size:        904    3277
 
XmaI C'CCGG_G

Cuts at:   1201    1201 
   Size:       4181
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AloI     AscI    AvrII
     BaeI     BcgI    BfrBI     BmtI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I     ClaI    CspCI   DraIII    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI    PflMI    PshAI     PsiI    PspXI
     PsrI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI
     ZraI
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