Restriction map for pF1KB0918
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaII       AviII       AvrII       
BalI        BanII       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfaI        BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaI        BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmFI       
BsmI        BspDI       BspEI       BspMI       BsrBI       
BsrDI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraII       
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        HphI        KpnI        KspI        
MaeI        MaeII       MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PflFI       PflMI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SmaI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
StyI        SwaI        Tsp45I      TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccII          66  CGCG                 CG^CG
AfaI          565  GTAC                 GT^AC
AflII         661  CTTAAG               C^TTAAG
AflIII        130  ACATGT               A^C[GA][CT]GT
AgeI           91  ACCGGT               A^CCGGT
AvaI           25  CCCGAG               C^[CT]CG[GA]G
BamHI         252  GGATCC               G^GATCC
BanI           40  GGCGCC               G^G[CT][GA]CC
BbeI           40  GGCGCC               GGCGC^C
BbsI          814  GAAGAC               GAAGAC(2/6)
BfrI          661  CTTAAG               C^TTAAG
BglI           15  GCCGCCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         951  AGATCT               A^GATCT
BpuAI         814  GAAGAC               GAAGAC(2/6)
BsaBI         247  GATCAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI          91  ACCGGT               [AT]^CCGG[AT]
BsiYI         547  CCCCTCTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          547  CCCCTCTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         751  GAGAC                GTCTC(1/5)
BsoBI          25  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      564  TGTACA               T^GTACA
BspHI         201  TCATGA               T^CATGA
BspLU11I      130  ACATGT               A^CATGT
BsrFI          91  ACCGGT               [GA]^CCGG[CT]
BsrGI         564  TGTACA               T^GTACA
BstBI         811  TTCGAA               TT^CGAA
BstNI           6  CCAGG                CC^[AT]GG
BstUI          66  CGCG                 CG^CG
Cfr10I         91  ACCGGT               [GA]^CCGG[CT]
DdeI          552  CTCAG                C^T[ACGT]AG
DraI          369  TTTAAA               TTT^AAA
DsaI          356  CCGTGG               C^C[GA][CT]GG
EcoRII          6  CCAGG                ^CC[AT]GG
EcoT22I       860  ATGCAT               ATGCA^T
FauI           64  CCCGC                CCCGC(4/6)
KasI           40  GGCGCC               G^GCGCC
MaeIII        380  GTAAC                ^GT[ACGT]AC
MamI          247  GATCAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          631  CACACAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI            6  CCAGG                CC^[AT]GG
MvnI           66  CGCG                 CG^CG
NarI           40  GGCGCC               GG^CGCC
NsiI          860  ATGCAT               ATGCA^T
NspV          811  TTCGAA               TT^CGAA
PinAI          91  ACCGGT               A^CCGGT
RcaI          201  TCATGA               T^CATGA
RsaI          565  GTAC                 GT^AC
SapI          616  GAAGAGC              GCTCTTC(1/4)
SfcI          801  CTGTAG               C^T[GA][CT]AG
SfoI           40  GGCGCC               GGC^GCC
SfuI          811  TTCGAA               TT^CGAA
SgrAI          90  CACCGGTG             C[GA]^CCGG[CT]G
SmlI          661  CTTAAG               C^T[CT][GA]AG
SspBI         564  TGTACA               T^GTACA
SspI          708  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AciI           16  CCGC                 CCGC(-3/-1)
               65  CCGC                
AcyI            9  GGCGTC               G[GA]^CG[CT]C
               40  GGCGCC              
AluI          195  AGCT                 AG^CT
              902  AGCT                
Alw44I        134  GTGCAC               G^TGCAC
              788  GTGCAC              
ApaLI         134  GTGCAC               G^TGCAC
              788  GTGCAC              
BsaHI           9  GGCGTC               G[GA]^CG[CT]C
               40  GGCGCC              
BseRI          79  CTCCTC               GAGGAG(10/8)
              347  GAGGAG              
BstYI         252  GGATCC               [GA]^GATC[CT]
              951  AGATCT              
EarI          616  GAAGAG               CTCTTC(1/4)
              892  GAAGAG              
HaeII          40  GGCGCC               [GA]GCGC^[CT]
               75  GGCGCT              
HgaI          418  GACGC                GACGC(5/10)
               10  GCGTC               
Hin1I           9  GGCGTC               G[GA]^CG[CT]C
               40  GGCGCC              
HinfI         286  GAATC                G^A[ACGT]TC
              763  GAATC               
Ksp632I       616  GAAGAG               CTCTTC(1/4)
              892  GAAGAG              
MflI          252  GGATCC               [GA]^GATC[CT]
              951  AGATCT              
NspI          130  ACATGT               [GA]CATG^[CT]
              474  ACATGC              
SfaNI         637  GATGC                GCATC(5/9)
              862  GCATC               
TfiI          286  GAATC                G^A[AT]TC
              763  GAATC               
XhoII         252  GGATCC               [GA]^GATC[CT]
              951  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AcsI        AlwI        ApoI        AspHI       BbvI        
BcnI        BmyI        BsaJI       BsiHKAI     Bsp1286I    
BssKI       BstF5I      Cac8I       CfoI        Cfr13I      
DpnII       Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        MboI        
MboII       MnlI        MseI        MspI        MwoI        
NciI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       TaqI        Tru9I       TseI        
Tsp509I     TthHB8I     
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