Restriction map for pF1KB0918
 (Linear) (Six-base) MAPSORT of: KIBB0918.seq  Check: 5219  from: 1  to: 993



KIBB0918.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     743     892     993 
   Size:        209     534     149     101

  Fragments arranged by size: 

                 534     209     149     101
 
AflII C'TTAA_G

Cuts at:      0     661     993 
   Size:        661     332
 
AflIII A'CryG_T

Cuts at:      0     130     993 
   Size:        130     863
 
AgeI A'CCGG_T

Cuts at:      0      91     993 
   Size:         91     902
 
ApaLI G'TGCA_C

Cuts at:      0     134     788     993 
   Size:        134     654     205

  Fragments arranged by size: 

                 654     205     134
 
ApoI r'AATT_y

Cuts at:      0     331     449     508     828     854     993 
   Size:        331     118      59     320      26     139

  Fragments arranged by size: 

                 331     320     139     118      59      26
 
AvaI C'yCGr_G

Cuts at:      0      25     993 
   Size:         25     968
 
BamHI G'GATC_C

Cuts at:      0     252     993 
   Size:        252     741
 
BanI G'GyrC_C

Cuts at:      0      40     993 
   Size:         40     953
 
BbeI G_GCGC'C

Cuts at:      0      44     993 
   Size:         44     949
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     821     993 
   Size:        821     172
 
BfrBI ATG'CAT

Cuts at:      0     862     993 
   Size:        862     131
 
BglI GCCn_nnn'nGGC

Cuts at:      0      21     993 
   Size:         21     972
 
BglII A'GATC_T

Cuts at:      0     951     993 
   Size:        951      42
 
Bme1580I G_kGCm'C

Cuts at:      0     138     792     993 
   Size:        138     654     201

  Fragments arranged by size: 

                 654     201     138
 
BsaBI GATnn'nnATC

Cuts at:      0     251     993 
   Size:        251     742
 
BsaHI Gr'CG_yC

Cuts at:      0      10      41     993 
   Size:         10      31     952

  Fragments arranged by size: 

                 952      31      10
 
BsaWI w'CCGG_w

Cuts at:      0      91     993 
   Size:         91     902
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     341     371     993 
   Size:        341      30     622

  Fragments arranged by size: 

                 622     341      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     362     993 
   Size:         70     292     631

  Fragments arranged by size: 

                 631     292      70
 
BsiHKAI G_wGCw'C

Cuts at:      0      38     138     792     934     993 
   Size:         38     100     654     142      59

  Fragments arranged by size: 

                 654     142     100      59      38
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     138     792     934     993 
   Size:         38     100     654     142      59

  Fragments arranged by size: 

                 654     142     100      59      38
 
BspHI T'CATG_A

Cuts at:      0     201     993 
   Size:        201     792
 
BsrFI r'CCGG_y

Cuts at:      0      91     993 
   Size:         91     902
 
BsrGI T'GTAC_A

Cuts at:      0     564     993 
   Size:        564     429
 
BstBI TT'CG_AA

Cuts at:      0     812     993 
   Size:        812     181
 
BstYI r'GATC_y

Cuts at:      0     252     951     993 
   Size:        252     699      42

  Fragments arranged by size: 

                 699     252      42
 
BtgI C'CryG_G

Cuts at:      0     356     993 
   Size:        356     637
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     477     993 
   Size:        477     516
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     856     891     993 
   Size:        856      35     102

  Fragments arranged by size: 

                 856     102      35
 
DraI TTT'AAA

Cuts at:      0     371     993 
   Size:        371     622
 
EarI CTCTTCn'nnn_

Cuts at:      0     611     887     993 
   Size:        611     276     106

  Fragments arranged by size: 

                 611     276     106
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     743     892     993 
   Size:        209     534     149     101

  Fragments arranged by size: 

                 534     209     149     101
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     700     993 
   Size:        700     293
 
HaeII r_GCGC'y

Cuts at:      0      44      79     993 
   Size:         44      35     914

  Fragments arranged by size: 

                 914      44      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     271     303     480     512     943     975     993 
   Size:        271      32     177      32     431      32      18

  Fragments arranged by size: 

                 431     271     177      32      32      32      18
 
KasI G'GCGC_C

Cuts at:      0      40     993 
   Size:         40     953
 
MslI CAynn'nnrTG

Cuts at:      0     635     993 
   Size:        635     358
 
NarI GG'CG_CC

Cuts at:      0      41     993 
   Size:         41     952
 
NsiI A_TGCA'T

Cuts at:      0     864     993 
   Size:        864     129
 
NspI r_CATG'y

Cuts at:      0     134     478     993 
   Size:        134     344     515

  Fragments arranged by size: 

                 515     344     134
 
PciI A'CATG_T

Cuts at:      0     130     993 
   Size:        130     863
 
PsiI TTA'TAA

Cuts at:      0     455     993 
   Size:        455     538
 
SapI GCTCTTCn'nnn_

Cuts at:      0     611     993 
   Size:        611     382
 
SfcI C'TryA_G

Cuts at:      0     801     993 
   Size:        801     192
 
SfoI GGC'GCC

Cuts at:      0      42     993 
   Size:         42     951
 
SgrAI Cr'CCGG_yG

Cuts at:      0      91     993 
   Size:         91     902
 
SmlI C'TyrA_G

Cuts at:      0     661     993 
   Size:        661     332
 
SspI AAT'ATT

Cuts at:      0     710     993 
   Size:        710     283
 
TatI w'GTAC_w

Cuts at:      0     564     993 
   Size:        564     429
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     341     373     993 
   Size:        341      32     620

  Fragments arranged by size: 

                 620     341      32
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AgeI    ApaLI     ApoI     AvaI    BamHI
     BanI     BbeI     BbsI    BfrBI     BglI    BglII Bme1580I    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI  BsiHKAI Bsp1286I    BspHI    BsrFI
    BsrGI    BstBI    BstYI     BtgI    BtgZI    CspCI     DraI     EarI
  Eco57MI     FalI    HaeII    Hin4I     KasI     MslI     NarI     NsiI
     NspI     PciI     PsiI     SapI     SfcI     SfoI    SgrAI     SmlI
     SspI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BanII    BbvCI     BcgI    BciVI     BclI     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrDI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I
   Bsu36I     BtsI     ClaI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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