Restriction map for pF1KB0912
 (Linear) (Six-base) MAPSORT of: KIBB0912.seq  Check: 5341  from: 1  to: 951



KIBB0912.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     419     951 
   Size:        419     532
 
AatII G_ACGT'C

Cuts at:      0     466     951 
   Size:        466     485
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     544     601     951 
   Size:        544      57     350

  Fragments arranged by size: 

                 544     350      57
 
AfeI AGC'GCT

Cuts at:      0     691     951 
   Size:        691     260
 
AflIII A'CryG_T

Cuts at:      0     154     221     951 
   Size:        154      67     730

  Fragments arranged by size: 

                 730     154      67
 
AlwNI CAG_nnn'CTG

Cuts at:      0     314     567     774     951 
   Size:        314     253     207     177

  Fragments arranged by size: 

                 314     253     207     177
 
BanI G'GyrC_C

Cuts at:      0      90     951 
   Size:         90     861
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     466     951 
   Size:        466     485
 
BbvCI CC'TCA_GC

Cuts at:      0     307     951 
   Size:        307     644
 
BglII A'GATC_T

Cuts at:      0      42     951 
   Size:         42     909
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     126     193     951 
   Size:        126      67     758

  Fragments arranged by size: 

                 758     126      67
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     475     589     658     951 
   Size:        385      90     114      69     293

  Fragments arranged by size: 

                 385     293     114      90      69
 
Bpu10I CC'TnA_GC

Cuts at:      0     307     951 
   Size:        307     644
 
BsaAI yAC'GTr

Cuts at:      0     825     951 
   Size:        825     126
 
BsaHI Gr'CG_yC

Cuts at:      0     463     951 
   Size:        463     488
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     448     478     951 
   Size:        448      30     473

  Fragments arranged by size: 

                 473     448      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      91     586     951 
   Size:         91     495     365

  Fragments arranged by size: 

                 495     365      91
 
BseYI C'CCAG_C

Cuts at:      0     638     951 
   Size:        638     313
 
BsiEI CG_ry'CG

Cuts at:      0     185     951 
   Size:        185     766
 
BsiHKAI G_wGCw'C

Cuts at:      0     323     674     951 
   Size:        323     351     277

  Fragments arranged by size: 

                 351     323     277
 
Bsp1286I G_dGCh'C

Cuts at:      0     323     674     951 
   Size:        323     351     277

  Fragments arranged by size: 

                 351     323     277
 
BspHI T'CATG_A

Cuts at:      0     920     951 
   Size:        920      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     419     951 
   Size:        419     532
 
BssSI C'ACGA_G

Cuts at:      0     751     951 
   Size:        751     200
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     122     951 
   Size:        122     829
 
BstYI r'GATC_y

Cuts at:      0      42     593     951 
   Size:         42     551     358

  Fragments arranged by size: 

                 551     358      42
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     429     951 
   Size:        429     522
 
BtsI GCAGTG_nn'

Cuts at:      0      69     549     951 
   Size:         69     480     402

  Fragments arranged by size: 

                 480     402      69
 
DraIII CAC_nnn'GTG

Cuts at:      0     134     951 
   Size:        134     817
 
EaeI y'GGCC_r

Cuts at:      0     776     827     951 
   Size:        776      51     124

  Fragments arranged by size: 

                 776     124      51
 
EarI CTCTTCn'nnn_

Cuts at:      0     938     951 
   Size:        938      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     505     951 
   Size:        505     446
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     475     544     589     601     658     951 
   Size:        385      90      69      45      12      57     293

  Fragments arranged by size: 

                 385     293      90      69      57      45      12
 
FspI TGC'GCA

Cuts at:      0     932     951 
   Size:        932      19
 
HaeII r_GCGC'y

Cuts at:      0     693     951 
   Size:        693     258
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     448     480     635     642     667     674     696     728 
   Size:        448      32     155       7      25       7      22      32

Cuts at:    728     951 
   Size:        223

  Fragments arranged by size: 

                 448     223     155      32      32      25      22       7
                   7
 
HincII GTy'rAC

Cuts at:      0      36     549     729     951 
   Size:         36     513     180     222

  Fragments arranged by size: 

                 513     222     180      36
 
MfeI C'AATT_G

Cuts at:      0     578     951 
   Size:        578     373
 
MscI TGG'CCA

Cuts at:      0     778     829     951 
   Size:        778      51     122

  Fragments arranged by size: 

                 778     122      51
 
MspA1I CmG'CkG

Cuts at:      0     469     486     513     638     951 
   Size:        469      17      27     125     313

  Fragments arranged by size: 

                 469     313     125      27      17
 
NspI r_CATG'y

Cuts at:      0     109     158     225     531     951 
   Size:        109      49      67     306     420

  Fragments arranged by size: 

                 420     306     109      67      49
 
PasI CC'CwG_GG

Cuts at:      0     768     951 
   Size:        768     183
 
PciI A'CATG_T

Cuts at:      0     154     221     951 
   Size:        154      67     730

  Fragments arranged by size: 

                 730     154      67
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     774     885     951 
   Size:        774     111      66

  Fragments arranged by size: 

                 774     111      66
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     793     825     951 
   Size:        793      32     126

  Fragments arranged by size: 

                 793     126      32
 
PstI C_TGCA'G

Cuts at:      0     239     951 
   Size:        239     712
 
PvuII CAG'CTG

Cuts at:      0     469     486     513     951 
   Size:        469      17      27     438

  Fragments arranged by size: 

                 469     438      27      17
 
SfcI C'TryA_G

Cuts at:      0     235     267     951 
   Size:        235      32     684

  Fragments arranged by size: 

                 684     235      32
 
StuI AGG'CCT

Cuts at:      0     914     951 
   Size:        914      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     145     594     951 
   Size:        145     449     357

  Fragments arranged by size: 

                 449     357     145
 
TatI w'GTAC_w

Cuts at:      0      84     951 
   Size:         84     867
 
ZraI GAC'GTC

Cuts at:      0     464     951 
   Size:        464     487
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII    AlwNI     BanI     BbsI
    BbvCI    BglII     BmrI     BpmI   Bpu10I    BsaAI    BsaHI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BssSI
   BstAPI    BstYI    BtgZI     BtsI   DraIII     EaeI     EarI     EciI
  Eco57MI     FspI    HaeII    Hin4I   HincII     MfeI     MscI   MspA1I
     NspI     PasI     PciI    PflMI     PsrI     PstI    PvuII     SfcI
     StuI    TaqII     TatI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI
     BsaI    BsaBI    BsaWI     BsgI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI     DrdI     EagI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
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