Restriction map for pF1KB0903
 (Circular) (Six-base) MAPSORT of: pF1KB0903.seq  Check: 7030  from: 1  to: 3383



pF1KB0903  3383 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AccI GT'mk_AC

Cuts at:    421     421 
   Size:       3383
 
Acc65I G'GTAC_C

Cuts at:    399     399 
   Size:       3383
 
AclI AA'CG_TT

Cuts at:   3223    3223 
   Size:       3383
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1208    1640    2387    2655    1208 
   Size:        432     747     268    1936

  Fragments arranged by size: 

                1936     747     432     268
 
AflIII A'CryG_T

Cuts at:    735    1839    2816     735 
   Size:       1104     977    1302

  Fragments arranged by size: 

                1302    1104     977
 
AgeI A'CCGG_T

Cuts at:   1771    1771 
   Size:       3383
 
AlwNI CAG_nnn'CTG

Cuts at:    688    2255     688 
   Size:       1567    1816
 
ApaLI G'TGCA_C

Cuts at:   2153    2153 
   Size:       3383
 
ApoI r'AATT_y

Cuts at:    140     319     387    2736    2992     140 
   Size:        179      68    2349     256     531

  Fragments arranged by size: 

                2349     531     256     179      68
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3383
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3383
 
AvaI C'yCGr_G

Cuts at:    269     403     269 
   Size:        134    3249
 
BamHI G'GATC_C

Cuts at:    408     408 
   Size:       3383
 
BanI G'GyrC_C

Cuts at:    399     813    1061    1096     399 
   Size:        414     248      35    2686

  Fragments arranged by size: 

                2686     414     248      35
 
BanII G_rGCy'C

Cuts at:    397    1427     397 
   Size:       1030    2353
 
BbeI G_GCGC'C

Cuts at:    817    1065     817 
   Size:        248    3135
 
BbsI GAAGACnn'nnnn_

Cuts at:    227     350     227 
   Size:        123    3260
 
BciVI GTATCCnnnnn_n'

Cuts at:   1281    2042    3383    1281 
   Size:        761    1341    1281

  Fragments arranged by size: 

                1341    1281     761
 
BclI T'GATC_A

Cuts at:    903     903 
   Size:       3383
 
BglII A'GATC_T

Cuts at:    898    2935     898 
   Size:       2037    1346
 
BlpI GC'TnA_GC

Cuts at:    495     495 
   Size:       3383
 
Bme1580I G_kGCm'C

Cuts at:   1008    1101    2157    1008 
   Size:         93    1056    2234

  Fragments arranged by size: 

                2234    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    595     676    1011    3155     595 
   Size:         81     335    2144     823

  Fragments arranged by size: 

                2144     823     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1750    1750 
   Size:       3383
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    559    1438    1930    2228     559 
   Size:        879     492     298    1714

  Fragments arranged by size: 

                1714     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3087    3087 
   Size:       3383
 
BsaAI yAC'GTr

Cuts at:    736     736 
   Size:       3383
 
BsaBI GATnn'nnATC

Cuts at:      6     897    2934       6 
   Size:        891    2037     455

  Fragments arranged by size: 

                2037     891     455
 
BsaHI Gr'CG_yC

Cuts at:    814    1062    1764    3260     814 
   Size:        248     702    1496     937

  Fragments arranged by size: 

                1496     937     702     248
 
BsaWI w'CCGG_w

Cuts at:    572    1093    1771    2045    2192    2603     572 
   Size:        521     678     274     147     411    1352

  Fragments arranged by size: 

                1352     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    808    1566    2143    2639     808 
   Size:        758     577     496    1552

  Fragments arranged by size: 

                1552     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    182     182 
   Size:       3383
 
BsiEI CG_ry'CG

Cuts at:    104     925     971    2179     104 
   Size:        821      46    1208    1308

  Fragments arranged by size: 

                1308    1208     821      46
 
BsiHKAI G_wGCw'C

Cuts at:    397    1175    1365    2157     397 
   Size:        778     190     792    1623

  Fragments arranged by size: 

                1623     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    397    1008    1101    1175    1365    1427    2157     397 
   Size:        611      93      74     190      62     730    1623

  Fragments arranged by size: 

                1623     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2603    2603 
   Size:       3383
 
BspHI T'CATG_A

Cuts at:   2559    2559 
   Size:       3383
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    433     647     949    1330     433 
   Size:        214     302     381    2486

  Fragments arranged by size: 

                2486     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1675    1729    3213    3378      50 
   Size:       1625      54    1484     165      55

  Fragments arranged by size: 

                1625    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1295    1295 
   Size:       3383
 
BsrFI r'CCGG_y

Cuts at:   1381    1562    1771    1381 
   Size:        181     209    2993

  Fragments arranged by size: 

                2993     209     181
 
BssSI C'ACGA_G

Cuts at:   1654    2012    1654 
   Size:        358    3025
 
BstAPI GCAn_nnn'nTGC

Cuts at:    760     760 
   Size:       3383
 
BstBI TT'CG_AA

Cuts at:   1744    1744 
   Size:       3383
 
BstYI r'GATC_y

Cuts at:      1     408     875     898    1233    1479    2491    2577    2589 
   Size:        407     467      23     335     246    1012      86      12

Cuts at:   2589    2935       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     449     407     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3383
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1333    1514    1677    1333 
   Size:        181     163    3039

  Fragments arranged by size: 

                3039     181     163
 
BtsI GCAGTG_nn'

Cuts at:    626     626 
   Size:       3383
 
DraI TTT'AAA

Cuts at:    382     382 
   Size:       3383
 
DrdI GACnn_nn'nnGTC

Cuts at:   1089    1947    1089 
   Size:        858    2525
 
EaeI y'GGCC_r

Cuts at:    968    1142    1533    1560    2905     968 
   Size:        174     391      27    1345    1446

  Fragments arranged by size: 

                1446    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:    968     968 
   Size:       3383
 
EarI CTCTTCn'nnn_

Cuts at:   1406    1616    1406 
   Size:        210    3173
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1591    1901    2047    3193    1591 
   Size:        310     146    1146    1781

  Fragments arranged by size: 

                1781    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    395     395 
   Size:       3383
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1208    1640    2387    2655    1208 
   Size:        432     747     268    1936

  Fragments arranged by size: 

                1936     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    196     196 
   Size:       3383
 
EcoO109I rG'GnC_Cy

Cuts at:    301     522     301 
   Size:        221    3162
 
EcoRI G'AATT_C

Cuts at:    387     387 
   Size:       3383
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1402    1402 
   Size:       3383
 
FspI TGC'GCA

Cuts at:   1164    1164 
   Size:       3383
 
HaeII r_GCGC'y

Cuts at:    817    1065    2087    3066     817 
   Size:        248    1022     979    1134

  Fragments arranged by size: 

                1134    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1225    1255    1257    1287    1225 
   Size:         30       2      30    3321

  Fragments arranged by size: 

                3321      30      30       2
 
HincII GTy'rAC

Cuts at:    422     422 
   Size:       3383
 
KasI G'GCGC_C

Cuts at:    813    1061     813 
   Size:        248    3135
 
KpnI G_GTAC'C

Cuts at:    403     403 
   Size:       3383
 
MluI A'CGCG_T

Cuts at:   2816    2816 
   Size:       3383
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2054    2238    2054 
   Size:        184    3199
 
MscI TGG'CCA

Cuts at:   1144    1144 
   Size:       3383
 
MslI CAynn'nnrTG

Cuts at:    256     335    1372    1499     256 
   Size:         79    1037     127    2140

  Fragments arranged by size: 

                2140    1037     127      79
 
MspA1I CmG'CkG

Cuts at:    494     808    1168    2181    2426     494 
   Size:        314     360    1013     245    1451

  Fragments arranged by size: 

                1451    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1564    1564 
   Size:       3383
 
NarI GG'CG_CC

Cuts at:    814    1062     814 
   Size:        248    3135
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3383
 
NgoMIV G'CCGG_C

Cuts at:   1562    1562 
   Size:       3383
 
NspI r_CATG'y

Cuts at:    436     936    1843     436 
   Size:        500     907    1976

  Fragments arranged by size: 

                1976     907     500
 
PciI A'CATG_T

Cuts at:   1839    1839 
   Size:       3383
 
PflMI CCAn_nnn'nTGG

Cuts at:    156     156 
   Size:       3383
 
PmeI GTTT'AAAC

Cuts at:    382     382 
   Size:       3383
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2522    2554    2522 
   Size:         32    3351
 
PpuMI rG'GwC_Cy

Cuts at:    301     301 
   Size:       3383
 
PshAI GACnn'nnGTC

Cuts at:    306     306 
   Size:       3383
 
PspXI vC'TCGA_Gb

Cuts at:    269     269 
   Size:       3383
 
PstI C_TGCA'G

Cuts at:    430    1115     430 
   Size:        685    2698
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3383
 
PvuII CAG'CTG

Cuts at:    808    1168     808 
   Size:        360    3023
 
RsrII CG'GwC_CG

Cuts at:   1578    1578 
   Size:       3383
 
SacI G_AGCT'C

Cuts at:    397     397 
   Size:       3383
 
SalI G'TCGA_C

Cuts at:    420     420 
   Size:       3383
 
SapI GCTCTTCn'nnn_

Cuts at:   1406    1616    1406 
   Size:        210    3173
 
SbfI CC_TGCA'GG

Cuts at:    430     430 
   Size:       3383
 
ScaI AGT'ACT

Cuts at:   2900    2900 
   Size:       3383
 
SfcI C'TryA_G

Cuts at:     33     426    1111    2104    2295      33 
   Size:        393     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     393     191
 
SfoI GGC'GCC

Cuts at:    815    1063     815 
   Size:        248    3135
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3383
 
SmaI CCC'GGG

Cuts at:    405     405 
   Size:       3383
 
SmlI C'TyrA_G

Cuts at:    269     538    1453    1945    2207     269 
   Size:        269     915     492     262    1445

  Fragments arranged by size: 

                1445     915     492     269     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3383
 
SphI G_CATG'C

Cuts at:    436     936     436 
   Size:        500    2883
 
StyI C'CwwG_G

Cuts at:    107     517     871     107 
   Size:        410     354    2619

  Fragments arranged by size: 

                2619     410     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    148     966    1099    1558    1767     148 
   Size:        818     133     459     209    1764

  Fragments arranged by size: 

                1764     818     459     209     133
 
TatI w'GTAC_w

Cuts at:   2898    2898 
   Size:       3383
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2900    2932    2900 
   Size:         32    3351
 
Tth111I GACn'n_nGTC

Cuts at:    253    1180     253 
   Size:        927    2456
 
XbaI T'CTAG_A

Cuts at:    414     414 
   Size:       3383
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    878     878 
   Size:       3383
 
XhoI C'TCGA_G

Cuts at:    269     269 
   Size:       3383
 
XmaI C'CCGG_G

Cuts at:    403     403 
   Size:       3383
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PmeI     PpiI
    PpuMI    PshAI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI
     PmlI     PsiI   PspOMI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XmnI     ZraI
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