Restriction map for pF1KB0473
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AgeI        AhdI        AlwI        AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AvrII       BamHI       BbeI        
BbrPI       BcgI        BciVI       BclI        BfaI        
BfrI        BlnI        BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspEI       BspHI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
Bsu36I      Cfr10I      CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HincII      HindII      HindIII     HpaI        
KasI        KspI        MaeI        MamI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TfiI        Tru9I       Tsp509I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I        856  GGTACC               G^GTACC
AcyI           48  GACGCC               G[GA]^CG[CT]C
AflIII        296  ACATGT               A^C[GA][CT]GT
Alw44I        517  GTGCAC               G^TGCAC
ApaLI         517  GTGCAC               G^TGCAC
Asp718I       856  GGTACC               G^GTACC
AspI          307  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1217  TGCGCA               TGC^GCA
BbsI          672  GTCTTC               GAAGAC(2/6)
BbvCI         475  GCTGAGG              CCTCAGC(-5/-2)
BglI         1402  GCCATCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1361  AGATCT               A^GATCT
BpuAI         672  GTCTTC               GAAGAC(2/6)
BsaHI          48  GACGCC               G[GA]^CG[CT]C
BsaI         1051  GGTCTC               GGTCTC(1/5)
BspDI        1243  ATCGAT               AT^CGAT
BspLU11I      296  ACATGT               A^CATGT
BsrBI        1105  GAGCGG               CCGCTC(-3/-3)
BstEII        993  GGTCACC              G^GT[ACGT]ACC
BstPI         993  GGTCACC              G^GT[ACGT]ACC
BstXI         942  CCAGGCACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1361  AGATCT               [GA]^GATC[CT]
ClaI         1243  ATCGAT               AT^CGAT
DraIII       1165  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI           92  CCATGG               C^C[GA][CT]GG
EarI         1081  GAAGAG               CTCTTC(1/4)
EcoNI          69  CCTTCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       993  GGTCACC              G^GT[ACGT]ACC
FspI         1217  TGCGCA               TGC^GCA
Hin1I          48  GACGCC               G[GA]^CG[CT]C
KpnI          856  GGTACC               GGTAC^C
Ksp632I      1081  GAAGAG               CTCTTC(1/4)
MflI         1361  AGATCT               [GA]^GATC[CT]
NcoI           92  CCATGG               C^CATGG
NspI          296  ACATGT               [GA]CATG^[CT]
PflFI         307  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         903  CCAGCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         875  GACTGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI          704  AGTACT               AGT^ACT
SfiI         1401  GGCCATCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          593  CCCGGG               CCC^GGG
Tth111I       307  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        903  CCAGCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1361  AGATCT               [GA]^GATC[CT]
XmaCI         593  CCCGGG               C^CCGGG
XmaI          593  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AccI          211  GTCTAC               GT^[AC][GT]AC
             1200  GTCTAC              
BanI          856  GGTACC               G^G[CT][GA]CC
              945  GGCACC              
BanII         219  GGGCTC               G[GA]GC[CT]^C
             1060  GAGCCC              
BlpI          175  GCTCAGC              GC^T[ACGT]AGC
             1119  GCTGAGC             
Bpu1102I      175  GCTCAGC              GC^T[ACGT]AGC
             1119  GCTGAGC             
BsaAI         214  TACGTG               [CT]AC^GT[GA]
              505  TACGTG              
BsgI          365  CTGCAC               GTGCAG(16/14)
              582  CTGCAC              
BsmAI        1390  GAGAC                GTCTC(1/5)
             1052  GTCTC               
BspMI         161  ACCTGC               ACCTGC(4/8)
              777  ACCTGC              
CelII         175  GCTCAGC              GC^T[ACGT]AGC
             1119  GCTGAGC             
FauI          186  GCGGG                CCCGC(4/6)
              711  GCGGG               
HaeII         189  GGCGCT               [GA]GCGC^[CT]
             1156  AGCGCC              
MaeII         215  ACGT                 A^CGT
              506  ACGT                
PstI          163  CTGCAG               CTGCA^G
              800  CTGCAG              
PvuII         370  CAGCTG               CAG^CTG
              794  CAGCTG              
TaqI          293  TCGA                 T^CGA
             1244  TCGA                
TthHB8I       293  TCGA                 T^CGA
             1244  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AspHI       AvaI        AvaII       BalI        BbvI        
BcnI        BmyI        BpmI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StuI        StyI        TseI        
Tsp45I      TspRI       XcmI        
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