Restriction map for pF1KB0463
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflIII      AgeI        AhdI        
Alw44I      AlwI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbrPI       BciVI       BfaI        BlnI        BsaBI       
BseAI       BseRI       BsgI        BsiWI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HindIII     HpaI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tsp509I     Tth111I     
XbaI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AflII        1395  CTTAAG               C^TTAAG
AocI          196  CCTGAGG              CC^T[ACGT]AGG
AspHI        1225  GAGCTC               G[AT]GC[AT]^C
BbvCI          46  CCTCAGC              CCTCAGC(-5/-2)
BclI          863  TGATCA               T^GATCA
BcnI         1248  CCCGG                CC^[GC]GG
BfrI         1395  CTTAAG               C^TTAAG
BglII        1433  AGATCT               A^GATCT
BsaAI         475  TACGTG               [CT]AC^GT[GA]
BsaI         1014  GGTCTC               GGTCTC(1/5)
BsaWI        1451  ACCGGA               [AT]^CCGG[AT]
BsiEI         872  CGGTCG               CG[GA][CT]^CG
BsiHKAI      1225  GAGCTC               G[AT]GC[AT]^C
BsmI          548  GCATTC               GAATGC(1/-1)
BsrDI         867  CATTGC               GCAATG(2/0)
BsrFI         851  ACCGGC               [GA]^CCGG[CT]
BstYI        1433  AGATCT               [GA]^GATC[CT]
Bsu36I        196  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        851  ACCGGC               [GA]^CCGG[CT]
EarI         1228  CTCTTC               CTCTTC(1/4)
Eco81I        196  CCTGAGG              CC^T[ACGT]AGG
FbaI          863  TGATCA               T^GATCA
Ksp632I      1228  CTCTTC               CTCTTC(1/4)
MaeIII         58  GTGAC                ^GT[ACGT]AC
MflI         1433  AGATCT               [GA]^GATC[CT]
MseI         1396  TTAA                 T^TAA
NciI         1248  CCCGG                CC^[GC]GG
NcoI         1256  CCATGG               C^CATGG
NspI         1076  GCATGT               [GA]CATG^[CT]
PshAI          66  GACCCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1225  GAGCTC               GAGCT^C
SapI         1227  GCTCTTC              GCTCTTC(1/4)
Tru9I        1396  TTAA                 T^TAA
Tsp45I         58  GTGAC                ^GT[GC]AC
XhoII        1433  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

ApaI          242  GGGCCC               GGGCC^C
             1336  GGGCCC              
BbsI         1003  GTCTTC               GAAGAC(2/6)
             1345  GTCTTC              
BcgI          708  CGAGGGGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              548  GCATTCTGATCG        
BlpI          772  GCTGAGC              GC^T[ACGT]AGC
              885  GCTGAGC             
Bpu1102I      772  GCTGAGC              GC^T[ACGT]AGC
              885  GCTGAGC             
BpuAI        1003  GTCTTC               GAAGAC(2/6)
             1345  GTCTTC              
BsmBI        1146  CGTCTC               CGTCTC(1/5)
              172  GAGACG              
BspMI         500  ACCTGC               ACCTGC(4/8)
              633  ACCTGC              
BsrBI         808  GAGCGG               CCGCTC(-3/-3)
             1033  GAGCGG              
BstXI         470  CCATGTACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1256  CCATGGCTGTGG        
CelII         772  GCTGAGC              GC^T[ACGT]AGC
              885  GCTGAGC             
HgaI          519  GACGC                GACGC(5/10)
             1050  GACGC               
HincII        340  GTCAAC               GT[CT]^[GA]AC
              994  GTCAAC              
HindII        340  GTCAAC               GT[CT]^[GA]AC
              994  GTCAAC              
PaeR7I        706  CTCGAG               C^TCGAG
             1412  CTCGAG              
PleI          577  GAGTC                GAGTC(4/5)
              726  GAGTC               
SfaNI         905  GCATC                GCATC(5/9)
              935  GCATC               
TfiI          425  GATTC                G^A[AT]TC
              702  GAATC               
XcmI          257  CCATCTTTTCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              891  CCAGCAGCTCTCTGG     
XhoI          706  CTCGAG               C^TCGAG
             1412  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwNI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbvI        BglI        BmyI        BpmI        
BsaHI       BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        MaeII       MboI        MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MwoI        NarI        NdeII       
NlaIII      NlaIV       PflMI       PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfcI        SfoI        SmlI        StyI        TaqI        
TseI        TspRI       TthHB8I     Van91I      
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