Restriction map for pF1KB0449
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BbvCI       BcgI        BfaI        
BfrI        BglI        BlnI        BsaBI       BseAI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstBI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoT22I     FseI        FspI        HgaI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
StuI        SwaI        TaqI        Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII         274  GACGTC               GACGT^C
AccII        1170  CGCG                 CG^CG
AcyI          274  GACGTC               G[GA]^CG[CT]C
Alw44I       1294  GTGCAC               G^TGCAC
ApaLI        1294  GTGCAC               G^TGCAC
BamHI         940  GGATCC               G^GATCC
BanI          457  GGCACC               G^G[CT][GA]CC
BciVI        1182  GGATAC               GTATCC(6/5)
BclI            2  TGATCA               T^GATCA
BglII         354  AGATCT               A^GATCT
BlpI          369  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      369  GCTCAGC              GC^T[ACGT]AGC
BsaAI         430  TACGTG               [CT]AC^GT[GA]
BsaHI         274  GACGTC               G[GA]^CG[CT]C
BsaWI        1274  TCCGGT               [AT]^CCGG[AT]
BsmBI        1087  GAGACG               CGTCTC(1/5)
BspMI         809  ACCTGC               ACCTGC(4/8)
BsrFI         116  ACCGGC               [GA]^CCGG[CT]
BssSI         535  CACGAG               CACGAG(-5/-1)
BstAPI       1246  GCAGAGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        903  GGTGACC              G^GT[ACGT]ACC
BstPI         903  GGTGACC              G^GT[ACGT]ACC
BstUI        1170  CGCG                 CG^CG
BstXI         893  CCAATGAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         369  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        116  ACCGGC               [GA]^CCGG[CT]
EcoNI         312  CCTGCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       903  GGTGACC              G^GT[ACGT]ACC
EcoRV         304  GATATC               GAT^ATC
FbaI            2  TGATCA               T^GATCA
Hin1I         274  GACGTC               G[GA]^CG[CT]C
MseI           26  TTAA                 T^TAA
MvnI         1170  CGCG                 CG^CG
PvuII         742  CAGCTG               CAG^CTG
ScaI          950  AGTACT               AGT^ACT
SmaI         1023  CCCGGG               CCC^GGG
SphI          713  GCATGC               GCATG^C
SspI          601  AATATT               AAT^ATT
Tru9I          26  TTAA                 T^TAA
XmaCI        1023  CCCGGG               C^CCGGG
XmaI         1023  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI           85  GAATTC               [GA]^AATT[CT]
              322  GAATTC              
ApoI           85  GAATTC               [GA]^AATT[CT]
              322  GAATTC              
AspHI         532  GAGCAC               G[AT]GC[AT]^C
             1294  GTGCAC              
AvaI         1023  CCCGGG               C^[CT]CG[GA]G
             1069  CTCGGG              
BalI          857  TGGCCA               TGG^CCA
             1001  TGGCCA              
BanII         348  GAGCCC               G[GA]GC[CT]^C
             1026  GGGCTC              
BbsI         1306  GAAGAC               GAAGAC(2/6)
              472  GTCTTC              
BpmI         1345  CTCCAG               CTGGAG(16/14)
              529  CTGGAG              
BpuAI        1306  GAAGAC               GAAGAC(2/6)
              472  GTCTTC              
BsiHKAI       532  GAGCAC               G[AT]GC[AT]^C
             1294  GTGCAC              
BsoBI        1023  CCCGGG               C^[CT]CG[GA]G
             1069  CTCGGG              
BspHI           5  TCATGA               T^CATGA
              707  TCATGA              
BsrBI         367  CCGCTC               CCGCTC(-3/-3)
             1156  CCGCTC              
DsaI          266  CCATGG               C^C[GA][CT]GG
              299  CCATGG              
EarI         1018  CTCTTC               CTCTTC(1/4)
             1084  GAAGAG              
EcoRI          85  GAATTC               G^AATTC
              322  GAATTC              
Ksp632I      1018  CTCTTC               CTCTTC(1/4)
             1084  GAAGAG              
MluNI         857  TGGCCA               TGG^CCA
             1001  TGGCCA              
MscI          857  TGGCCA               TGG^CCA
             1001  TGGCCA              
NcoI          266  CCATGG               C^CATGG
              299  CCATGG              
PleI         1233  GAGTC                GAGTC(4/5)
             1260  GAGTC               
RcaI            5  TCATGA               T^CATGA
              707  TCATGA              
TfiI           73  GAATC                G^A[AT]TC
             1225  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BbvI        BcnI        BmyI        BsaI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PpuMI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TseI        
Tsp45I      Tsp509I     TspRI       XhoII       
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