Restriction map for pF1KB0435
 (Circular) (Six-base) MAPSORT of: pF1KB0435.seq  Check: 1330  from: 1  to: 4457



pF1KB0435  4457 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1434    1434 
   Size:       4457
 
AccI GT'mk_AC

Cuts at:    959    1495     959 
   Size:        536    3921
 
Acc65I G'GTAC_C

Cuts at:   1473    1473 
   Size:       4457
 
AclI AA'CG_TT

Cuts at:   4297    4297 
   Size:       4457
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    548     760    1276    2282    2714    3461    3729     548 
   Size:        212     516    1006     432     747     268    1276

  Fragments arranged by size: 

                1276    1006     747     516     432     268     212
 
AflIII A'CryG_T

Cuts at:   1112    1809    2913    3890    1112 
   Size:        697    1104     977    1679

  Fragments arranged by size: 

                1679    1104     977     697
 
AgeI A'CCGG_T

Cuts at:   2845    2845 
   Size:       4457
 
AlwNI CAG_nnn'CTG

Cuts at:    945    1762    3329     945 
   Size:        817    1567    2073

  Fragments arranged by size: 

                2073    1567     817
 
ApaLI G'TGCA_C

Cuts at:   3227    3227 
   Size:       4457
 
ApoI r'AATT_y

Cuts at:    868    1461    3810    4066     868 
   Size:        593    2349     256    1259

  Fragments arranged by size: 

                2349    1259     593     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4457
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4457
 
AvaI C'yCGr_G

Cuts at:    179     594     780    1165    1477     179 
   Size:        415     186     385     312    3159

  Fragments arranged by size: 

                3159     415     385     312     186
 
BamHI G'GATC_C

Cuts at:   1482    1482 
   Size:       4457
 
BanI G'GyrC_C

Cuts at:    295     528     589    1229    1473    1887    2135    2170     295 
   Size:        233      61     640     244     414     248      35    2582

  Fragments arranged by size: 

                2582     640     414     248     244     233      61      35
            
 
BanII G_rGCy'C

Cuts at:    275     977     997    1471    2501     275 
   Size:        702      20     474    1030    2231

  Fragments arranged by size: 

                2231    1030     702     474      20
 
BbeI G_GCGC'C

Cuts at:   1233    1891    2139    1233 
   Size:        658     248    3551

  Fragments arranged by size: 

                3551     658     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    748    1177     748 
   Size:        429    4028
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    901     935    1087    1121     901 
   Size:         34     152      34    4237

  Fragments arranged by size: 

                4237     152      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2355    3116    4457    2355 
   Size:        761    1341    2355

  Fragments arranged by size: 

                2355    1341     761
 
BclI T'GATC_A

Cuts at:    671    1977     671 
   Size:       1306    3151
 
BglI GCCn_nnn'nGGC

Cuts at:    461     461 
   Size:       4457
 
BglII A'GATC_T

Cuts at:   1972    4009    1972 
   Size:       2037    2420
 
BlpI GC'TnA_GC

Cuts at:    174     946    1186    1569     174 
   Size:        772     240     383    3062

  Fragments arranged by size: 

                3062     772     383     240
 
Bme1580I G_kGCm'C

Cuts at:    250    2082    2175    3231     250 
   Size:       1832      93    1056    1476

  Fragments arranged by size: 

                1832    1476    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1669    1750    2085    4229    1669 
   Size:         81     335    2144    1897

  Fragments arranged by size: 

                2144    1897     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2824    2824 
   Size:       4457
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    496     875    1423     496 
   Size:        379     548    3530

  Fragments arranged by size: 

                3530     548     379
 
Bpu10I CC'TnA_GC

Cuts at:   1334    1334 
   Size:       4457
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1633    2512    3004    3302    1633 
   Size:        879     492     298    2788

  Fragments arranged by size: 

                2788     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4161    4161 
   Size:       4457
 
BsaAI yAC'GTr

Cuts at:   1196    1810    1196 
   Size:        614    3843
 
BsaBI GATnn'nnATC

Cuts at:      6    1971    4008       6 
   Size:       1965    2037     455

  Fragments arranged by size: 

                2037    1965     455
 
BsaHI Gr'CG_yC

Cuts at:    136     372    1230    1888    2136    2838    4334     136 
   Size:        236     858     658     248     702    1496     259

  Fragments arranged by size: 

                1496     858     702     658     259     248     236
 
BsaWI w'CCGG_w

Cuts at:   1646    2167    2845    3119    3266    3677    1646 
   Size:        521     678     274     147     411    2426

  Fragments arranged by size: 

                2426     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    597     627     873     903    1372    1402     597 
   Size:         30     246      30     469      30    3652

  Fragments arranged by size: 

                3652     469     246      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    283     832     985     283 
   Size:        549     153    3755

  Fragments arranged by size: 

                3755     549     153
 
BseYI C'CCAG_C

Cuts at:    759    1433    1882    2640    3217    3713     759 
   Size:        674     449     758     577     496    1503

  Fragments arranged by size: 

                1503     758     674     577     496     449
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    111    1375    1412     111 
   Size:       1264      37    3156

  Fragments arranged by size: 

                3156    1264      37
 
BsiEI CG_ry'CG

Cuts at:    104    1999    2045    3253     104 
   Size:       1895      46    1208    1308

  Fragments arranged by size: 

                1895    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    175    1471    2249    2439    3231     175 
   Size:       1296     778     190     792    1401

  Fragments arranged by size: 

                1401    1296     792     778     190
 
BsmI GAATG_Cn'

Cuts at:   1187    1187 
   Size:       4457
 
Bsp1286I G_dGCh'C

Cuts at:    175     250     275     977     997    1471    2082    2175    2249 
   Size:         75      25     702      20     474     611      93      74

Cuts at:   2249    2439    2501    3231     175 
   Size:        190      62     730    1401

  Fragments arranged by size: 

                1401     730     702     611     474     190      93      75
                  74      62      25      20
 
BspEI T'CCGG_A

Cuts at:   3677    3677 
   Size:       4457
 
BspHI T'CATG_A

Cuts at:   3633    3633 
   Size:       4457
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1434    1507    1721    2023    2404    1434 
   Size:         73     214     302     381    3487

  Fragments arranged by size: 

                3487     381     302     214      73
 
BsrBI CCG'CTC

Cuts at:     50     184    1365    2749    2803    4287    4452      50 
   Size:        134    1181    1384      54    1484     165      55

  Fragments arranged by size: 

                1484    1384    1181     165     134      55      54
 
BsrDI GCAATG_nn'

Cuts at:    674     686    2369     674 
   Size:         12    1683    2762

  Fragments arranged by size: 

                2762    1683      12
 
BsrFI r'CCGG_y

Cuts at:   2455    2636    2845    2455 
   Size:        181     209    4067

  Fragments arranged by size: 

                4067     209     181
 
BssSI C'ACGA_G

Cuts at:   2728    3086    2728 
   Size:        358    4099
 
BstAPI GCAn_nnn'nTGC

Cuts at:    509    1834     509 
   Size:       1325    3132
 
BstBI TT'CG_AA

Cuts at:   2818    2818 
   Size:       4457
 
BstYI r'GATC_y

Cuts at:      1    1091    1482    1949    1972    2307    2553    3565    3651 
   Size:       1090     391     467      23     335     246    1012      86

Cuts at:   3651    3663    4009       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1090    1012     467     449     391     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107    1142     107 
   Size:       1035    3422
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    690    2407    2588    2751     690 
   Size:       1717     181     163    2396

  Fragments arranged by size: 

                2396    1717     181     163
 
BtsI GCAGTG_nn'

Cuts at:    662    1700     662 
   Size:       1038    3419
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1098    1133    1098 
   Size:         35    4422
 
DraI TTT'AAA

Cuts at:   1456    1456 
   Size:       4457
 
DrdI GACnn_nn'nnGTC

Cuts at:   2163    3021    2163 
   Size:        858    3599
 
EaeI y'GGCC_r

Cuts at:    659     701    1031    2042    2216    2607    2634    3979     659 
   Size:         42     330    1011     174     391      27    1345    1137

  Fragments arranged by size: 

                1345    1137    1011     391     330     174      42      27
            
 
EagI C'GGCC_G

Cuts at:   2042    2042 
   Size:       4457
 
EarI CTCTTCn'nnn_

Cuts at:    781    1252    2480    2690     781 
   Size:        471    1228     210    2548

  Fragments arranged by size: 

                2548    1228     471     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    349    2665    2975    3121    4267     349 
   Size:       2316     310     146    1146     539

  Fragments arranged by size: 

                2316    1146     539     310     146
 
EcoICRI GAG'CTC

Cuts at:   1469    1469 
   Size:       4457
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    496     548     760     875    1276    1423    2282    2714    3461 
   Size:         52     212     115     401     147     859     432     747

Cuts at:   3461    3729     496 
   Size:        268    1224

  Fragments arranged by size: 

                1224     859     747     432     401     268     212     147
                 115      52
 
EcoO109I rG'GnC_Cy

Cuts at:    831    1596     831 
   Size:        765    3692
 
EcoRI G'AATT_C

Cuts at:   1461    1461 
   Size:       4457
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2476    2476 
   Size:       4457
 
FspI TGC'GCA

Cuts at:   2238    2238 
   Size:       4457
 
HaeII r_GCGC'y

Cuts at:   1233    1891    2139    3161    4140    1233 
   Size:        658     248    1022     979    1550

  Fragments arranged by size: 

                1550    1022     979     658     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    873     905     910     942    2299    2329    2331    2361     873 
   Size:         32       5      32    1357      30       2      30    2969

  Fragments arranged by size: 

                2969    1357      32      32      30      30       5       2
            
 
HincII GTy'rAC

Cuts at:    960    1496     960 
   Size:        536    3921
 
KasI G'GCGC_C

Cuts at:   1229    1887    2135    1229 
   Size:        658     248    3551

  Fragments arranged by size: 

                3551     658     248
 
KpnI G_GTAC'C

Cuts at:   1477    1477 
   Size:       4457
 
MluI A'CGCG_T

Cuts at:   3890    3890 
   Size:       4457
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3128    3312    3128 
   Size:        184    4273
 
MscI TGG'CCA

Cuts at:    661     703    1033    2218     661 
   Size:         42     330    1185    2900

  Fragments arranged by size: 

                2900    1185     330      42
 
MslI CAynn'nnrTG

Cuts at:    679     724    2446    2573     679 
   Size:         45    1722     127    2563

  Fragments arranged by size: 

                2563    1722     127      45
 
MspA1I CmG'CkG

Cuts at:    942    1568    1882    2242    3255    3500     942 
   Size:        626     314     360    1013     245    1899

  Fragments arranged by size: 

                1899    1013     626     360     314     245
 
NaeI GCC'GGC

Cuts at:   2638    2638 
   Size:       4457
 
NarI GG'CG_CC

Cuts at:   1230    1888    2136    1230 
   Size:        658     248    3551

  Fragments arranged by size: 

                3551     658     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4457
 
NgoMIV G'CCGG_C

Cuts at:   2636    2636 
   Size:       4457
 
NspI r_CATG'y

Cuts at:    474    1293    1444    1510    2010    2917     474 
   Size:        819     151      66     500     907    2014

  Fragments arranged by size: 

                2014     907     819     500     151      66
 
PciI A'CATG_T

Cuts at:   2913    2913 
   Size:       4457
 
PmeI GTTT'AAAC

Cuts at:   1456    1456 
   Size:       4457
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    907     939    3596    3628     907 
   Size:         32    2657      32    1736

  Fragments arranged by size: 

                2657    1736      32      32
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    199     231     199 
   Size:         32    4425
 
PstI C_TGCA'G

Cuts at:   1504    2189    1504 
   Size:        685    3772
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4457
 
PvuII CAG'CTG

Cuts at:    942    1882    2242     942 
   Size:        940     360    3157

  Fragments arranged by size: 

                3157     940     360
 
RsrII CG'GwC_CG

Cuts at:   2652    2652 
   Size:       4457
 
SacI G_AGCT'C

Cuts at:   1471    1471 
   Size:       4457
 
SalI G'TCGA_C

Cuts at:    958    1494     958 
   Size:        536    3921
 
SapI GCTCTTCn'nnn_

Cuts at:    781    2480    2690     781 
   Size:       1699     210    2548

  Fragments arranged by size: 

                2548    1699     210
 
SbfI CC_TGCA'GG

Cuts at:   1504    1504 
   Size:       4457
 
ScaI AGT'ACT

Cuts at:    214    3974     214 
   Size:       3760     697
 
SfcI C'TryA_G

Cuts at:     33    1323    1500    2185    3178    3369      33 
   Size:       1290     177     685     993     191    1121

  Fragments arranged by size: 

                1290    1121     993     685     191     177
 
SfoI GGC'GCC

Cuts at:   1231    1889    2137    1231 
   Size:        658     248    3551

  Fragments arranged by size: 

                3551     658     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4457
 
SmaI CCC'GGG

Cuts at:    782    1479     782 
   Size:        697    3760
 
SmlI C'TyrA_G

Cuts at:   1165    1612    2527    3019    3281    1165 
   Size:        447     915     492     262    2341

  Fragments arranged by size: 

                2341     915     492     447     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4457
 
SphI G_CATG'C

Cuts at:   1293    1510    2010    1293 
   Size:        217     500    3740

  Fragments arranged by size: 

                3740     500     217
 
StyI C'CwwG_G

Cuts at:    107     542     648    1591    1945     107 
   Size:        435     106     943     354    2619

  Fragments arranged by size: 

                2619     943     435     354     106
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    501    2040    2173    2632    2841     501 
   Size:       1539     133     459     209    2117

  Fragments arranged by size: 

                2117    1539     459     209     133
 
TatI w'GTAC_w

Cuts at:    212    3972     212 
   Size:       3760     697
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    597     629    1372    1404    3974    4006     597 
   Size:         32     743      32    2570      32    1048

  Fragments arranged by size: 

                2570    1048     743      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2254    2254 
   Size:       4457
 
XbaI T'CTAG_A

Cuts at:   1488    1488 
   Size:       4457
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114     549     696    1952     114 
   Size:        435     147    1256    2619

  Fragments arranged by size: 

                2619    1256     435     147
 
XhoI C'TCGA_G

Cuts at:   1165    1165 
   Size:       4457
 
XmaI C'CCGG_G

Cuts at:    780    1477     780 
   Size:        697    3760
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI     PmeI     PpiI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI    BfrBI    BmgBI     BmtI    BsiWI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XmnI     ZraI
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