Restriction map for pF1KB0411
 (Circular) (Six-base) MAPSORT of: pF1KB0411.seq  Check: 5955  from: 1  to: 4514



pF1KB0411  4514 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AccI GT'mk_AC

Cuts at:    563    1552     563 
   Size:        989    3525
 
Acc65I G'GTAC_C

Cuts at:    315    1530     315 
   Size:       1215    3299
 
AclI AA'CG_TT

Cuts at:   4354    4354 
   Size:       4514
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2339    2771    3518    3786    2339 
   Size:        432     747     268    3067

  Fragments arranged by size: 

                3067     747     432     268
 
AfeI AGC'GCT

Cuts at:   1096    1096 
   Size:       4514
 
AflIII A'CryG_T

Cuts at:   1866    2970    3947    1866 
   Size:       1104     977    2433

  Fragments arranged by size: 

                2433    1104     977
 
AgeI A'CCGG_T

Cuts at:   2902    2902 
   Size:       4514
 
AhdI GACnn_n'nnGTC

Cuts at:    254     254 
   Size:       4514
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1191    1354    1191 
   Size:        163    4351
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    510     542     734     766     510 
   Size:         32     192      32    4258

  Fragments arranged by size: 

                4258     192      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   1308    1819    3386    1308 
   Size:        511    1567    2436

  Fragments arranged by size: 

                2436    1567     511
 
ApaLI G'TGCA_C

Cuts at:   1389    3284    1389 
   Size:       1895    2619
 
ApoI r'AATT_y

Cuts at:    428     787    1518    3867    4123     428 
   Size:        359     731    2349     256     819

  Fragments arranged by size: 

                2349     819     731     359     256
 
AseI AT'TA_AT

Cuts at:     20     157      20 
   Size:        137    4377
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4514
 
AvaI C'yCGr_G

Cuts at:    542    1534     542 
   Size:        992    3522
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    307     340     307 
   Size:         33    4481
 
BamHI G'GATC_C

Cuts at:   1539    1539 
   Size:       4514
 
BanI G'GyrC_C

Cuts at:    315     547    1075    1530    1944    2192    2227     315 
   Size:        232     528     455     414     248      35    2602

  Fragments arranged by size: 

                2602     528     455     414     248     232      35
 
BanII G_rGCy'C

Cuts at:    994    1528    2558     994 
   Size:        534    1030    2950

  Fragments arranged by size: 

                2950    1030     534
 
BbeI G_GCGC'C

Cuts at:    551    1948    2196     551 
   Size:       1397     248    2869

  Fragments arranged by size: 

                2869    1397     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1166    1166 
   Size:       4514
 
BciVI GTATCCnnnnn_n'

Cuts at:   2412    3173    4514    2412 
   Size:        761    1341    2412

  Fragments arranged by size: 

                2412    1341     761
 
BclI T'GATC_A

Cuts at:   2034    2034 
   Size:       4514
 
BglII A'GATC_T

Cuts at:   2029    4066    2029 
   Size:       2037    2477
 
BlpI GC'TnA_GC

Cuts at:   1107    1626    1107 
   Size:        519    3995
 
Bme1580I G_kGCm'C

Cuts at:   1078    1393    2139    2232    3288    1078 
   Size:        315     746      93    1056    2304

  Fragments arranged by size: 

                2304    1056     746     315      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    233     610    1726    1807    2142    4286     233 
   Size:        377    1116      81     335    2144     461

  Fragments arranged by size: 

                2144    1116     461     377     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2881    2881 
   Size:       4514
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    775     784    1234     775 
   Size:          9     450    4055

  Fragments arranged by size: 

                4055     450       9
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    805    1405    1690    2569    3061    3359     805 
   Size:        600     285     879     492     298    1960

  Fragments arranged by size: 

                1960     879     600     492     298     285
 
BsaI GGTCTCn'nnnn_

Cuts at:   4218    4218 
   Size:       4514
 
BsaAI yAC'GTr

Cuts at:   1867    1867 
   Size:       4514
 
BsaBI GATnn'nnATC

Cuts at:      6    2028    4065       6 
   Size:       2022    2037     455

  Fragments arranged by size: 

                2037    2022     455
 
BsaHI Gr'CG_yC

Cuts at:    548    1945    2193    2895    4391     548 
   Size:       1397     248     702    1496     671

  Fragments arranged by size: 

                1496    1397     702     671     248
 
BsaWI w'CCGG_w

Cuts at:   1703    2224    2902    3176    3323    3734    1703 
   Size:        521     678     274     147     411    2483

  Fragments arranged by size: 

                2483     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    734     764     734 
   Size:         30    4484
 
BseYI C'CCAG_C

Cuts at:   1939    2697    3274    3770    1939 
   Size:        758     577     496    2683

  Fragments arranged by size: 

                2683     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1029    1029 
   Size:       4514
 
BsiEI CG_ry'CG

Cuts at:    104    2056    2102    3310     104 
   Size:       1952      46    1208    1308

  Fragments arranged by size: 

                1952    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    124    1393    1528    2306    2496    3288     124 
   Size:       1269     135     778     190     792    1350

  Fragments arranged by size: 

                1350    1269     792     778     190     135
 
Bsp1286I G_dGCh'C

Cuts at:    124     994    1078    1393    1528    2139    2232    2306    2496 
   Size:        870      84     315     135     611      93      74     190

Cuts at:   2496    2558    3288     124 
   Size:         62     730    1350

  Fragments arranged by size: 

                1350     870     730     611     315     190     135      93
                  84      74      62
 
BspEI T'CCGG_A

Cuts at:   3734    3734 
   Size:       4514
 
BspHI T'CATG_A

Cuts at:    861    3690     861 
   Size:       2829    1685
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1564    1778    2080    2461    1564 
   Size:        214     302     381    3617

  Fragments arranged by size: 

                3617     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2806    2860    4344    4509      50 
   Size:       2756      54    1484     165      55

  Fragments arranged by size: 

                2756    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1163    1373    2426    1163 
   Size:        210    1053    3251

  Fragments arranged by size: 

                3251    1053     210
 
BsrFI r'CCGG_y

Cuts at:   2512    2693    2902    2512 
   Size:        181     209    4124

  Fragments arranged by size: 

                4124     209     181
 
BsrGI T'GTAC_A

Cuts at:   1127    1127 
   Size:       4514
 
BssSI C'ACGA_G

Cuts at:    667    2785    3143     667 
   Size:       2118     358    2038

  Fragments arranged by size: 

                2118    2038     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1891    1891 
   Size:       4514
 
BstBI TT'CG_AA

Cuts at:   2875    2875 
   Size:       4514
 
BstYI r'GATC_y

Cuts at:      1    1539    2006    2029    2364    2610    3622    3708    3720 
   Size:       1538     467      23     335     246    1012      86      12

Cuts at:   3720    4066       1 
   Size:        346     449

  Fragments arranged by size: 

                1538    1012     467     449     346     335     246      86
                  23      12
 
BstZ17I GTA'TAC

Cuts at:    564     564 
   Size:       4514
 
Bsu36I CC'TnA_GG

Cuts at:    959     959 
   Size:       4514
 
BtgI C'CryG_G

Cuts at:    107     319     107 
   Size:        212    4302
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    660    2464    2645    2808     660 
   Size:       1804     181     163    2366

  Fragments arranged by size: 

                2366    1804     181     163
 
BtsI GCAGTG_nn'

Cuts at:    386    1757     386 
   Size:       1371    3143
 
DraI TTT'AAA

Cuts at:    170    1513     170 
   Size:       1343    3171
 
DrdI GACnn_nn'nnGTC

Cuts at:   2220    3078    2220 
   Size:        858    3656
 
EaeI y'GGCC_r

Cuts at:    306    2099    2273    2664    2691    4036     306 
   Size:       1793     174     391      27    1345     784

  Fragments arranged by size: 

                1793    1345     784     391     174      27
 
EagI C'GGCC_G

Cuts at:   2099    2099 
   Size:       4514
 
EarI CTCTTCn'nnn_

Cuts at:    137     212    2537    2747     137 
   Size:         75    2325     210    1904

  Fragments arranged by size: 

                2325    1904     210      75
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2722    3032    3178    4324    2722 
   Size:        310     146    1146    2912

  Fragments arranged by size: 

                2912    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1526    1526 
   Size:       4514
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    775     784    1234    2339    2771    3518    3786     775 
   Size:          9     450    1105     432     747     268    1503

  Fragments arranged by size: 

                1503    1105     747     450     432     268       9
 
EcoO109I rG'GnC_Cy

Cuts at:    963    1266    1653     963 
   Size:        303     387    3824

  Fragments arranged by size: 

                3824     387     303
 
EcoRI G'AATT_C

Cuts at:   1518    1518 
   Size:       4514
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    582    2533     582 
   Size:       1951    2563
 
FspI TGC'GCA

Cuts at:   2295    2295 
   Size:       4514
 
HaeII r_GCGC'y

Cuts at:    551    1098    1457    1948    2196    3218    4197     551 
   Size:        547     359     491     248    1022     979     868

  Fragments arranged by size: 

                1022     979     868     547     491     359     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    240     272     730     762    2356    2386    2388    2418     240 
   Size:         32     458      32    1594      30       2      30    2336

  Fragments arranged by size: 

                2336    1594     458      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1553    1553 
   Size:       4514
 
HindIII A'AGCT_T

Cuts at:   1205    1205 
   Size:       4514
 
KasI G'GCGC_C

Cuts at:    547    1944    2192     547 
   Size:       1397     248    2869

  Fragments arranged by size: 

                2869    1397     248
 
KpnI G_GTAC'C

Cuts at:    319    1534     319 
   Size:       1215    3299
 
MluI A'CGCG_T

Cuts at:   3947    3947 
   Size:       4514
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    407    3185    3369     407 
   Size:       2778     184    1552

  Fragments arranged by size: 

                2778    1552     184
 
MscI TGG'CCA

Cuts at:    308    2275     308 
   Size:       1967    2547
 
MslI CAynn'nnrTG

Cuts at:    170     401    1063    2503    2630     170 
   Size:        231     662    1440     127    2054

  Fragments arranged by size: 

                2054    1440     662     231     127
 
MspA1I CmG'CkG

Cuts at:   1625    1939    2299    3312    3557    1625 
   Size:        314     360    1013     245    2582

  Fragments arranged by size: 

                2582    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2695    2695 
   Size:       4514
 
NarI GG'CG_CC

Cuts at:    548    1945    2193     548 
   Size:       1397     248    2869

  Fragments arranged by size: 

                2869    1397     248
 
NcoI C'CATG_G

Cuts at:    107     319     107 
   Size:        212    4302
 
NgoMIV G'CCGG_C

Cuts at:   2693    2693 
   Size:       4514
 
NspI r_CATG'y

Cuts at:    817    1068    1567    2067    2974     817 
   Size:        251     499     500     907    2357

  Fragments arranged by size: 

                2357     907     500     499     251
 
PacI TTA_AT'TAA

Cuts at:    161     161 
   Size:       4514
 
PasI CC'CwG_GG

Cuts at:   1085    1085 
   Size:       4514
 
PciI A'CATG_T

Cuts at:   2970    2970 
   Size:       4514
 
PmeI GTTT'AAAC

Cuts at:   1513    1513 
   Size:       4514
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    734     766    3653    3685     734 
   Size:         32    2887      32    1563

  Fragments arranged by size: 

                2887    1563      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    963     963 
   Size:       4514
 
PshAI GACnn'nnGTC

Cuts at:   1178    1178 
   Size:       4514
 
PstI C_TGCA'G

Cuts at:   1180    1561    2246    1180 
   Size:        381     685    3448

  Fragments arranged by size: 

                3448     685     381
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4514
 
PvuII CAG'CTG

Cuts at:   1939    2299    1939 
   Size:        360    4154
 
RsrII CG'GwC_CG

Cuts at:   2709    2709 
   Size:       4514
 
SacI G_AGCT'C

Cuts at:   1528    1528 
   Size:       4514
 
SalI G'TCGA_C

Cuts at:   1551    1551 
   Size:       4514
 
SapI GCTCTTCn'nnn_

Cuts at:    212    2537    2747     212 
   Size:       2325     210    1979

  Fragments arranged by size: 

                2325    1979     210
 
SbfI CC_TGCA'GG

Cuts at:   1561    1561 
   Size:       4514
 
ScaI AGT'ACT

Cuts at:   4031    4031 
   Size:       4514
 
SfcI C'TryA_G

Cuts at:     33    1038    1176    1557    2242    3235    3426      33 
   Size:       1005     138     381     685     993     191    1121

  Fragments arranged by size: 

                1121    1005     993     685     381     191     138
 
SfoI GGC'GCC

Cuts at:    549    1946    2194     549 
   Size:       1397     248    2869

  Fragments arranged by size: 

                2869    1397     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4514
 
SmaI CCC'GGG

Cuts at:   1536    1536 
   Size:       4514
 
SmlI C'TyrA_G

Cuts at:    820    1420    1669    2584    3076    3338     820 
   Size:        600     249     915     492     262    1996

  Fragments arranged by size: 

                1996     915     600     492     262     249
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4514
 
SphI G_CATG'C

Cuts at:   1567    2067    1567 
   Size:        500    4014
 
SspI AAT'ATT

Cuts at:    522     522 
   Size:       4514
 
StyI C'CwwG_G

Cuts at:    107     319    1648    2002     107 
   Size:        212    1329     354    2619

  Fragments arranged by size: 

                2619    1329     354     212
 
SwaI ATTT'AAAT

Cuts at:    170     170 
   Size:       4514
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    792     955    1077    2097    2230    2689    2898     792 
   Size:        163     122    1020     133     459     209    2408

  Fragments arranged by size: 

                2408    1020     459     209     163     133     122
 
TatI w'GTAC_w

Cuts at:    113    1127    4029     113 
   Size:       1014    2902     598

  Fragments arranged by size: 

                2902    1014     598
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4031    4063    4031 
   Size:         32    4482
 
Tth111I GACn'n_nGTC

Cuts at:   2311    2311 
   Size:       4514
 
XbaI T'CTAG_A

Cuts at:   1545    1545 
   Size:       4514
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2009    2009 
   Size:       4514
 
XmaI C'CCGG_G

Cuts at:   1534    1534 
   Size:       4514
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PacI     PasI
     PciI     PmeI     PpiI    PpuMI    PshAI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AleI     ApaI     AscI    AvrII    BbvCI
     BcgI    BfrBI     BglI    BmgBI     BmtI   Bpu10I    BseRI    BsiWI
     BsmI    BsmBI   BssHII   BstEII    BstXI     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI    PflMI     PfoI     PmlI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     StuI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com