Restriction map for
pF1KB6115
(Circular) (Six-base) MAPSORT of: pF1KB6115.seq Check: 8502 from: 1 to: 4973
pF1KB6115 4973 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 750 750
Size: 4973
AccI GT'mk_AC
Cuts at: 2011 2011
Size: 4973
Acc65I G'GTAC_C
Cuts at: 1989 1989
Size: 4973
AclI AA'CG_TT
Cuts at: 4813 4813
Size: 4973
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 849 1719 2798 3230 3977 4245 849
Size: 870 1079 432 747 268 1577
Fragments arranged by size:
1577 1079 870 747 432 268
AflIII A'CryG_T
Cuts at: 2325 3429 4406 2325
Size: 1104 977 2892
Fragments arranged by size:
2892 1104 977
AgeI A'CCGG_T
Cuts at: 3361 3361
Size: 4973
AleI CACnn'nnGTG
Cuts at: 1057 1057
Size: 4973
AlwNI CAG_nnn'CTG
Cuts at: 872 1428 1694 2278 3845 872
Size: 556 266 584 1567 2000
Fragments arranged by size:
2000 1567 584 556 266
ApaI G_GGCC'C
Cuts at: 184 184
Size: 4973
ApaLI G'TGCA_C
Cuts at: 3743 3743
Size: 4973
ApoI r'AATT_y
Cuts at: 1977 4326 4582 1977
Size: 2349 256 2368
Fragments arranged by size:
2368 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4973
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4973
AvaI C'yCGr_G
Cuts at: 711 1897 1993 711
Size: 1186 96 3691
Fragments arranged by size:
3691 1186 96
BamHI G'GATC_C
Cuts at: 638 1998 638
Size: 1360 3613
BanI G'GyrC_C
Cuts at: 394 1821 1989 2403 2651 2686 394
Size: 1427 168 414 248 35 2681
Fragments arranged by size:
2681 1427 414 248 168 35
BanII G_rGCy'C
Cuts at: 170 184 200 268 632 1409 1448 1987 3017
Size: 14 16 68 364 777 39 539 1030
Cuts at: 3017 170
Size: 2126
Fragments arranged by size:
2126 1030 777 539 364 68 39 16
14
BbeI G_GCGC'C
Cuts at: 2407 2655 2407
Size: 248 4725
BbsI GAAGACnn'nnnn_
Cuts at: 134 892 1191 1329 1512 134
Size: 758 299 138 183 3595
Fragments arranged by size:
3595 758 299 183 138
BbvCI CC'TCA_GC
Cuts at: 1320 1320
Size: 4973
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 288 322 288
Size: 34 4939
BciVI GTATCCnnnnn_n'
Cuts at: 2871 3632 4973 2871
Size: 761 1341 2871
Fragments arranged by size:
2871 1341 761
BclI T'GATC_A
Cuts at: 1397 2493 1397
Size: 1096 3877
BglII A'GATC_T
Cuts at: 506 2488 4525 506
Size: 1982 2037 954
Fragments arranged by size:
2037 1982 954
BlpI GC'TnA_GC
Cuts at: 2085 2085
Size: 4973
Bme1580I G_kGCm'C
Cuts at: 184 1241 1826 2598 2691 3747 184
Size: 1057 585 772 93 1056 1410
Fragments arranged by size:
1410 1057 1056 772 585 93
BmgBI CAC'GTC
Cuts at: 1251 1553 1251
Size: 302 4671
BmrI ACTGGGnnnn_n'
Cuts at: 1782 2185 2266 2601 4745 1782
Size: 403 81 335 2144 2010
Fragments arranged by size:
2144 2010 403 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 247 3340 247
Size: 3093 1880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 681 992 1089 681
Size: 311 97 4565
Fragments arranged by size:
4565 311 97
Bpu10I CC'TnA_GC
Cuts at: 161 1000 1320 161
Size: 839 320 3814
Fragments arranged by size:
3814 839 320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1945 2149 3028 3520 3818 1945
Size: 204 879 492 298 3100
Fragments arranged by size:
3100 879 492 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 283 4677 283
Size: 4394 579
BsaAI yAC'GTr
Cuts at: 930 2326 930
Size: 1396 3577
BsaBI GATnn'nnATC
Cuts at: 6 683 1279 2487 4524 6
Size: 677 596 1208 2037 455
Fragments arranged by size:
2037 1208 677 596 455
BsaHI Gr'CG_yC
Cuts at: 1030 2404 2652 3354 4850 1030
Size: 1374 248 702 1496 1153
Fragments arranged by size:
1496 1374 1153 702 248
BsaWI w'CCGG_w
Cuts at: 2162 2683 3361 3635 3782 4193 2162
Size: 521 678 274 147 411 2942
Fragments arranged by size:
2942 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 603 1173 603
Size: 570 4403
BseYI C'CCAG_C
Cuts at: 433 1654 1785 1790 2398 3156 3733 4229 433
Size: 1221 131 5 608 758 577 496 1177
Fragments arranged by size:
1221 1177 758 608 577 496 131 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 583 757 1414 247
Size: 336 174 657 3806
Fragments arranged by size:
3806 657 336 174
BsiEI CG_ry'CG
Cuts at: 104 2515 2561 3769 104
Size: 2411 46 1208 1308
Fragments arranged by size:
2411 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 200 632 1448 1574 1987 2765 2955 3747 200
Size: 432 816 126 413 778 190 792 1426
Fragments arranged by size:
1426 816 792 778 432 413 190 126
Bsp1286I G_dGCh'C
Cuts at: 170 184 200 268 632 1241 1409 1448 1574
Size: 14 16 68 364 609 168 39 126
Cuts at: 1574 1826 1987 2598 2691 2765 2955 3017 3747
Size: 252 161 611 93 74 190 62 730
Cuts at: 3747 170
Size: 1396
Fragments arranged by size:
1396 730 611 609 364 252 190 168
161 126 93 74 68 62 39 16
14
BspEI T'CCGG_A
Cuts at: 4193 4193
Size: 4973
BspHI T'CATG_A
Cuts at: 4149 4149
Size: 4973
BspMI ACCTGCnnnn'nnnn_
Cuts at: 422 750 2023 2237 2539 2920 422
Size: 328 1273 214 302 381 2475
Fragments arranged by size:
2475 1273 381 328 302 214
BsrBI CCG'CTC
Cuts at: 50 3265 3319 4803 4968 50
Size: 3215 54 1484 165 55
Fragments arranged by size:
3215 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 428 1889 2885 428
Size: 1461 996 2516
Fragments arranged by size:
2516 1461 996
BsrFI r'CCGG_y
Cuts at: 1016 1546 2971 3152 3361 1016
Size: 530 1425 181 209 2628
Fragments arranged by size:
2628 1425 530 209 181
BssSI C'ACGA_G
Cuts at: 3244 3602 3244
Size: 358 4615
BstAPI GCAn_nnn'nTGC
Cuts at: 1236 2350 1236
Size: 1114 3859
BstBI TT'CG_AA
Cuts at: 3334 3334
Size: 4973
BstEII G'GTnAC_C
Cuts at: 960 960
Size: 4973
BstXI CCAn_nnnn'nTGG
Cuts at: 177 790 1590 1785 1818 177
Size: 613 800 195 33 3332
Fragments arranged by size:
3332 800 613 195 33
BstYI r'GATC_y
Cuts at: 1 506 638 1998 2465 2488 2823 3069 4081
Size: 505 132 1360 467 23 335 246 1012
Cuts at: 4081 4167 4179 4525 1
Size: 86 12 346 449
Fragments arranged by size:
1360 1012 505 467 449 346 335 246
132 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 891 891
Size: 4973
BtgI C'CryG_G
Cuts at: 128 146 176 256 269 623 789 814 1331
Size: 18 30 80 13 354 166 25 517
Cuts at: 1331 1376 1811 1817 128
Size: 45 435 6 3284
Fragments arranged by size:
3284 517 435 354 166 80 45 30
25 18 13 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 570 1284 1782 2923 3104 3267 570
Size: 714 498 1141 181 163 2276
Fragments arranged by size:
2276 1141 714 498 181 163
BtsI GCAGTG_nn'
Cuts at: 642 938 2216 642
Size: 296 1278 3399
Fragments arranged by size:
3399 1278 296
ClaI AT'CG_AT
Cuts at: 1412 1412
Size: 4973
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 369 404 369
Size: 35 4938
DraI TTT'AAA
Cuts at: 1972 1972
Size: 4973
DraIII CAC_nnn'GTG
Cuts at: 1236 1236
Size: 4973
DrdI GACnn_nn'nnGTC
Cuts at: 1147 2679 3537 1147
Size: 1532 858 2583
Fragments arranged by size:
2583 1532 858
EaeI y'GGCC_r
Cuts at: 347 468 1049 1253 1814 2558 2732 3123 3150
Size: 121 581 204 561 744 174 391 27
Cuts at: 3150 4495 347
Size: 1345 825
Fragments arranged by size:
1345 825 744 581 561 391 204 174
121 27
EagI C'GGCC_G
Cuts at: 2558 2558
Size: 4973
EarI CTCTTCn'nnn_
Cuts at: 424 613 1453 1462 2996 3206 424
Size: 189 840 9 1534 210 2191
Fragments arranged by size:
2191 1534 840 210 189 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1939 3181 3491 3637 4783 1939
Size: 1242 310 146 1146 2129
Fragments arranged by size:
2129 1242 1146 310 146
EcoICRI GAG'CTC
Cuts at: 198 630 1446 1985 198
Size: 432 816 539 3186
Fragments arranged by size:
3186 816 539 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 681 849 992 1089 1719 2798 3230 3977 4245
Size: 168 143 97 630 1079 432 747 268
Cuts at: 4245 681
Size: 1409
Fragments arranged by size:
1409 1079 747 630 432 268 168 143
97
EcoNI CCTnn'n_nnAGG
Cuts at: 208 874 208
Size: 666 4307
EcoO109I rG'GnC_Cy
Cuts at: 808 1862 2112 808
Size: 1054 250 3669
Fragments arranged by size:
3669 1054 250
EcoRI G'AATT_C
Cuts at: 1977 1977
Size: 4973
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 327 2992 327
Size: 2665 2308
FspI TGC'GCA
Cuts at: 2754 2754
Size: 4973
HaeII r_GCGC'y
Cuts at: 598 1376 2407 2655 3677 4656 598
Size: 778 1031 248 1022 979 915
Fragments arranged by size:
1031 1022 979 915 778 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1559 1591 1745 1777 2815 2845 2847 2877 1559
Size: 32 154 32 1038 30 2 30 3655
Fragments arranged by size:
3655 1038 154 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1008 2012 1008
Size: 1004 3969
KasI G'GCGC_C
Cuts at: 2403 2651 2403
Size: 248 4725
KpnI G_GTAC'C
Cuts at: 1993 1993
Size: 4973
MluI A'CGCG_T
Cuts at: 4406 4406
Size: 4973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3644 3828 3644
Size: 184 4789
MscI TGG'CCA
Cuts at: 349 1255 1816 2734 349
Size: 906 561 918 2588
Fragments arranged by size:
2588 918 906 561
MslI CAynn'nnrTG
Cuts at: 689 1057 1588 1816 2962 3089 689
Size: 368 531 228 1146 127 2573
Fragments arranged by size:
2573 1146 531 368 228 127
MspA1I CmG'CkG
Cuts at: 258 493 816 1425 1650 2084 2398 2758 3771
Size: 235 323 609 225 434 314 360 1013
Cuts at: 3771 4016 258
Size: 245 1215
Fragments arranged by size:
1215 1013 609 434 360 323 314 245
235 225
NaeI GCC'GGC
Cuts at: 1548 3154 1548
Size: 1606 3367
NarI GG'CG_CC
Cuts at: 2404 2652 2404
Size: 248 4725
NcoI C'CATG_G
Cuts at: 789 1376 1811 1817 789
Size: 587 435 6 3945
Fragments arranged by size:
3945 587 435 6
NgoMIV G'CCGG_C
Cuts at: 1546 3152 1546
Size: 1606 3367
NspI r_CATG'y
Cuts at: 2026 2526 3433 2026
Size: 500 907 3566
Fragments arranged by size:
3566 907 500
PasI CC'CwG_GG
Cuts at: 1071 1359 1071
Size: 288 4685
PciI A'CATG_T
Cuts at: 3429 3429
Size: 4973
PflMI CCAn_nnn'nTGG
Cuts at: 356 356
Size: 4973
PfoI T'CCnGG_A
Cuts at: 655 1529 655
Size: 874 4099
PmeI GTTT'AAAC
Cuts at: 1972 1972
Size: 4973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 4112
Size: 32 4941
PpuMI rG'GwC_Cy
Cuts at: 1862 1862
Size: 4973
PshAI GACnn'nnGTC
Cuts at: 138 138
Size: 4973
PspOMI G'GGCC_C
Cuts at: 180 180
Size: 4973
PstI C_TGCA'G
Cuts at: 1852 2020 2705 1852
Size: 168 685 4120
Fragments arranged by size:
4120 685 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4973
PvuII CAG'CTG
Cuts at: 1425 2398 2758 1425
Size: 973 360 3640
Fragments arranged by size:
3640 973 360
RsrII CG'GwC_CG
Cuts at: 3168 3168
Size: 4973
SacI G_AGCT'C
Cuts at: 200 632 1448 1987 200
Size: 432 816 539 3186
Fragments arranged by size:
3186 816 539 432
SacII CC_GC'GG
Cuts at: 259 817 259
Size: 558 4415
SalI G'TCGA_C
Cuts at: 2010 2010
Size: 4973
SapI GCTCTTCn'nnn_
Cuts at: 424 1453 1462 2996 3206 424
Size: 1029 9 1534 210 2191
Fragments arranged by size:
2191 1534 1029 210 9
SbfI CC_TGCA'GG
Cuts at: 2020 2020
Size: 4973
ScaI AGT'ACT
Cuts at: 754 4490 754
Size: 3736 1237
SfcI C'TryA_G
Cuts at: 33 1134 1848 2016 2701 3694 3885 33
Size: 1101 714 168 685 993 191 1121
Fragments arranged by size:
1121 1101 993 714 685 191 168
SfoI GGC'GCC
Cuts at: 2405 2653 2405
Size: 248 4725
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4973
SmaI CCC'GGG
Cuts at: 1995 1995
Size: 4973
SmlI C'TyrA_G
Cuts at: 1960 2128 3043 3535 3797 1960
Size: 168 915 492 262 3136
Fragments arranged by size:
3136 915 492 262 168
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4973
SphI G_CATG'C
Cuts at: 2026 2526 2026
Size: 500 4473
StyI C'CwwG_G
Cuts at: 789 1256 1376 1811 1817 2107 2461 789
Size: 467 120 435 6 290 354 3301
Fragments arranged by size:
3301 467 435 354 290 120 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1069 1369 2556 2689 3148 3357 1069
Size: 300 1187 133 459 209 2685
Fragments arranged by size:
2685 1187 459 300 209 133
TatI w'GTAC_w
Cuts at: 752 1661 4488 752
Size: 909 2827 1237
Fragments arranged by size:
2827 1237 909
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4490 4522 4490
Size: 32 4941
Tth111I GACn'n_nGTC
Cuts at: 2770 2770
Size: 4973
XbaI T'CTAG_A
Cuts at: 2004 2004
Size: 4973
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 787 927 1248 1383 1809 2468 787
Size: 140 321 135 426 659 3292
Fragments arranged by size:
3292 659 426 321 140 135
XmaI C'CCGG_G
Cuts at: 1993 1993
Size: 4973
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BfrBI BglI BmtI BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI