Restriction map for pF1KB8511
 (Linear) (Six-base) MAPSORT of: KIBB8511.seq  Check: 9997  from: 1  to: 2823



KIBB8511.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1570    1692    2823 
   Size:       1570     122    1131

  Fragments arranged by size: 

                1570    1131     122
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1268    2332    2395    2521    2823 
   Size:       1268    1064      63     126     302

  Fragments arranged by size: 

                1268    1064     302     126      63
 
AfeI AGC'GCT

Cuts at:      0    2698    2823 
   Size:       2698     125
 
AhdI GACnn_n'nnGTC

Cuts at:      0     796    2823 
   Size:        796    2027
 
AleI CACnn'nnGTG

Cuts at:      0    1297    2232    2823 
   Size:       1297     935     591

  Fragments arranged by size: 

                1297     935     591
 
ApaI G_GGCC'C

Cuts at:      0     107    1983    2823 
   Size:        107    1876     840

  Fragments arranged by size: 

                1876     840     107
 
ApaLI G'TGCA_C

Cuts at:      0    1673    2823 
   Size:       1673    1150
 
ApoI r'AATT_y

Cuts at:      0     831    2823 
   Size:        831    1992
 
AvaI C'yCGr_G

Cuts at:      0     481    1189    1766    2024    2279    2458    2823 
   Size:        481     708     577     258     255     179     365

  Fragments arranged by size: 

                 708     577     481     365     258     255     179
 
AvrII C'CTAG_G

Cuts at:      0      24    2823 
   Size:         24    2799
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2415    2448    2823 
   Size:       2415      33     375

  Fragments arranged by size: 

                2415     375      33
 
BamHI G'GATC_C

Cuts at:      0     600    1517    2823 
   Size:        600     917    1306

  Fragments arranged by size: 

                1306     917     600
 
BanI G'GyrC_C

Cuts at:      0      70    1840    2823 
   Size:         70    1770     983

  Fragments arranged by size: 

                1770     983      70
 
BanII G_rGCy'C

Cuts at:      0     107    1307    1983    2823 
   Size:        107    1200     676     840

  Fragments arranged by size: 

                1200     840     676     107
 
BbeI G_GCGC'C

Cuts at:      0      74    1844    2823 
   Size:         74    1770     979

  Fragments arranged by size: 

                1770     979      74
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2260    2294    2823 
   Size:       2260      34     529

  Fragments arranged by size: 

                2260     529      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2443    2823 
   Size:       2443     380
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1906    2823 
   Size:       1906     917
 
BglII A'GATC_T

Cuts at:      0    1928    2085    2823 
   Size:       1928     157     738

  Fragments arranged by size: 

                1928     738     157
 
BlpI GC'TnA_GC

Cuts at:      0     472     763    1267    1412    2823 
   Size:        472     291     504     145    1411

  Fragments arranged by size: 

                1411     504     472     291     145
 
Bme1580I G_kGCm'C

Cuts at:      0     107    1018    1677    1983    2016    2422    2823 
   Size:        107     911     659     306      33     406     401

  Fragments arranged by size: 

                 911     659     406     401     306     107      33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     974    2823 
   Size:        974    1849
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     761    2823 
   Size:        761    2062
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1348    1438    1687    1972    2071    2275    2377    2823 
   Size:       1348      90     249     285      99     204     102     446

  Fragments arranged by size: 

                1348     446     285     249     204     102      99      90
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     418     877    2823 
   Size:        252     166     459    1946

  Fragments arranged by size: 

                1946     459     252     166
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1324    2823 
   Size:       1324    1499
 
BsaAI yAC'GTr

Cuts at:      0      51    2823 
   Size:         51    2772
 
BsaBI GATnn'nnATC

Cuts at:      0    1126    2823 
   Size:       1126    1697
 
BsaHI Gr'CG_yC

Cuts at:      0      71     679    1841    2823 
   Size:         71     608    1162     982

  Fragments arranged by size: 

                1162     982     608      71
 
BsaWI w'CCGG_w

Cuts at:      0     577    2428    2823 
   Size:        577    1851     395

  Fragments arranged by size: 

                1851     577     395
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2719    2749    2823 
   Size:       2719      30      74

  Fragments arranged by size: 

                2719      74      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     869     880    1618    1939    2110    2170    2272    2490 
   Size:        869      11     738     321     171      60     102     218

Cuts at:   2490    2686    2698    2823 
   Size:        196      12     125

  Fragments arranged by size: 

                 869     738     321     218     196     171     125     102
                  60      12      11
 
BseYI C'CCAG_C

Cuts at:      0      60     253    1546    1711    2823 
   Size:         60     193    1293     165    1112

  Fragments arranged by size: 

                1293    1112     193     165      60
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2729    2823 
   Size:       2729      94
 
BsiEI CG_ry'CG

Cuts at:      0    1464    2805    2823 
   Size:       1464    1341      18

  Fragments arranged by size: 

                1464    1341      18
 
BsiHKAI G_wGCw'C

Cuts at:      0    1307    1677    2286    2531    2823 
   Size:       1307     370     609     245     292

  Fragments arranged by size: 

                1307     609     370     292     245
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     901    2823 
   Size:        901    1922
 
Bsp1286I G_dGCh'C

Cuts at:      0     107    1018    1307    1677    1983    2016    2286    2422 
   Size:        107     911     289     370     306      33     270     136

Cuts at:   2422    2531    2823 
   Size:        109     292

  Fragments arranged by size: 

                 911     370     306     292     289     270     136     109
                 107      33
 
BspHI T'CATG_A

Cuts at:      0     995    2033    2823 
   Size:        995    1038     790

  Fragments arranged by size: 

                1038     995     790
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1570    1692    2823 
   Size:       1570     122    1131

  Fragments arranged by size: 

                1570    1131     122
 
BsrBI CCG'CTC

Cuts at:      0    1977    2823 
   Size:       1977     846
 
BsrFI r'CCGG_y

Cuts at:      0    2800    2823 
   Size:       2800      23
 
BssHII G'CGCG_C

Cuts at:      0    1734    2823 
   Size:       1734    1089
 
BssSI C'ACGA_G

Cuts at:      0    1676    2823 
   Size:       1676    1147
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1080    1299    1494    1857    2490    2823 
   Size:       1080     219     195     363     633     333

  Fragments arranged by size: 

                1080     633     363     333     219     195
 
BstYI r'GATC_y

Cuts at:      0     213     600    1517    1928    2085    2823 
   Size:        213     387     917     411     157     738

  Fragments arranged by size: 

                 917     738     411     387     213     157
 
Bsu36I CC'TnA_GG

Cuts at:      0    2788    2823 
   Size:       2788      35
 
BtgI C'CryG_G

Cuts at:      0    1079    1758    2537    2823 
   Size:       1079     679     779     286

  Fragments arranged by size: 

                1079     779     679     286
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     506    2367    2739    2823 
   Size:        506    1861     372      84

  Fragments arranged by size: 

                1861     506     372      84
 
BtsI GCAGTG_nn'

Cuts at:      0    1519    1806    2016    2823 
   Size:       1519     287     210     807

  Fragments arranged by size: 

                1519     807     287     210
 
DraI TTT'AAA

Cuts at:      0     830    2823 
   Size:        830    1993
 
EaeI y'GGCC_r

Cuts at:      0     729    1556    1994    2399    2534    2540    2798    2802 
   Size:        729     827     438     405     135       6     258       4

Cuts at:   2802    2823 
   Size:         21

  Fragments arranged by size: 

                 827     729     438     405     258     135      21       6
                   4
 
EagI C'GGCC_G

Cuts at:      0    2802    2823 
   Size:       2802      21
 
EarI CTCTTCn'nnn_

Cuts at:      0     268     458    1714    2823 
   Size:        268     190    1256    1109

  Fragments arranged by size: 

                1256    1109     268     190
 
EcoICRI GAG'CTC

Cuts at:      0    1305    2823 
   Size:       1305    1518
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1268    1348    1438    1687    1972    2071    2275    2332 
   Size:       1268      80      90     249     285      99     204      57

Cuts at:   2332    2377    2395    2521    2823 
   Size:         45      18     126     302

  Fragments arranged by size: 

                1268     302     285     249     204     126      99      90
                  80      57      45      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     701    2823 
   Size:        701    2122
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2100    2468    2823 
   Size:       2100     368     355

  Fragments arranged by size: 

                2100     368     355
 
EcoRV GAT'ATC

Cuts at:      0     870    2823 
   Size:        870    1953
 
FseI GG_CCGG'CC

Cuts at:      0    2804    2823 
   Size:       2804      19
 
HaeII r_GCGC'y

Cuts at:      0      74    1844    2410    2700    2823 
   Size:         74    1770     566     290     123

  Fragments arranged by size: 

                1770     566     290     123      74
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     782     814     845     875     877     900     907     932 
   Size:        782      32      31      30       2      23       7      25

Cuts at:    932    2823 
   Size:       1891

  Fragments arranged by size: 

                1891     782      32      31      30      25      23       7
                   2
 
HincII GTy'rAC

Cuts at:      0     804    2823 
   Size:        804    2019
 
KasI G'GCGC_C

Cuts at:      0      70    1840    2823 
   Size:         70    1770     983

  Fragments arranged by size: 

                1770     983      70
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     438    2321    2823 
   Size:        438    1883     502

  Fragments arranged by size: 

                1883     502     438
 
MscI TGG'CCA

Cuts at:      0     731    1558    1996    2401    2542    2823 
   Size:        731     827     438     405     141     281

  Fragments arranged by size: 

                 827     731     438     405     281     141
 
MslI CAynn'nnrTG

Cuts at:      0     748    1297    1441    2232    2823 
   Size:        748     549     144     791     591

  Fragments arranged by size: 

                 791     748     591     549     144
 
MspA1I CmG'CkG

Cuts at:      0     513    2562    2823 
   Size:        513    2049     261

  Fragments arranged by size: 

                2049     513     261
 
NaeI GCC'GGC

Cuts at:      0    2802    2823 
   Size:       2802      21
 
NarI GG'CG_CC

Cuts at:      0      71    1841    2823 
   Size:         71    1770     982

  Fragments arranged by size: 

                1770     982      71
 
NcoI C'CATG_G

Cuts at:      0    1758    2823 
   Size:       1758    1065
 
NgoMIV G'CCGG_C

Cuts at:      0    2800    2823 
   Size:       2800      23
 
NspI r_CATG'y

Cuts at:      0    1198    2823 
   Size:       1198    1625
 
PasI CC'CwG_GG

Cuts at:      0    2329    2823 
   Size:       2329     494
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2132    2585    2823 
   Size:       2132     453     238

  Fragments arranged by size: 

                2132     453     238
 
PfoI T'CCnGG_A

Cuts at:      0    1520    2823 
   Size:       1520    1303
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     577     609    2823 
   Size:        577      32    2214

  Fragments arranged by size: 

                2214     577      32
 
PspOMI G'GGCC_C

Cuts at:      0     103    1979    2823 
   Size:        103    1876     844

  Fragments arranged by size: 

                1876     844     103
 
PspXI vC'TCGA_Gb

Cuts at:      0    1189    2279    2458    2823 
   Size:       1189    1090     179     365

  Fragments arranged by size: 

                1189    1090     365     179
 
PstI C_TGCA'G

Cuts at:      0    1703    1793    1878    2414    2660    2823 
   Size:       1703      90      85     536     246     163

  Fragments arranged by size: 

                1703     536     246     163      90      85
 
SacI G_AGCT'C

Cuts at:      0    1307    2823 
   Size:       1307    1516
 
SbfI CC_TGCA'GG

Cuts at:      0    1703    2823 
   Size:       1703    1120
 
SfcI C'TryA_G

Cuts at:      0    1382    1699    1789    1874    2410    2656    2823 
   Size:       1382     317      90      85     536     246     167

  Fragments arranged by size: 

                1382     536     317     246     167      90      85
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1906    2823 
   Size:       1906     917
 
SfoI GGC'GCC

Cuts at:      0      72    1842    2823 
   Size:         72    1770     981

  Fragments arranged by size: 

                1770     981      72
 
SmaI CCC'GGG

Cuts at:      0    2026    2823 
   Size:       2026     797
 
SmlI C'TyrA_G

Cuts at:      0     267     433     892    1189    2279    2458    2823 
   Size:        267     166     459     297    1090     179     365

  Fragments arranged by size: 

                1090     459     365     297     267     179     166
 
StyI C'CwwG_G

Cuts at:      0      24    1073    1758    1997    2402    2573    2747    2823 
   Size:         24    1049     685     239     405     171     174      76

  Fragments arranged by size: 

                1049     685     405     239     174     171      76      24
            
 
SwaI ATTT'AAAT

Cuts at:      0     830    2823 
   Size:        830    1993
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1403    1478    2046    2823 
   Size:       1403      75     568     777

  Fragments arranged by size: 

                1403     777     568      75
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     624     656    2685    2717    2719    2751    2823 
   Size:        624      32    2029      32       2      32      72

  Fragments arranged by size: 

                2029     624      72      32      32      32       2
 
XhoI C'TCGA_G

Cuts at:      0    1189    2279    2458    2823 
   Size:       1189    1090     179     365

  Fragments arranged by size: 

                1189    1090     365     179
 
XmaI C'CCGG_G

Cuts at:      0    2024    2823 
   Size:       2024     799
 
XmnI GAAnn'nnTTC

Cuts at:      0      19    2818    2823 
   Size:         19    2799       5

  Fragments arranged by size: 

                2799      19       5
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AhdI     AleI     ApaI    ApaLI     ApoI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BcgI
    BciVI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrFI   BssHII    BssSI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRV     FseI    HaeII    Hin4I   HincII     KasI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI    PflMI     PfoI     PpiI   PspOMI    PspXI     PstI
     SacI     SbfI     SfcI     SfiI     SfoI     SmaI     SmlI     StyI
     SwaI    TaqII     TstI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AlfI
     AloI    AlwNI     AscI     AseI    AsiSI     BbsI    BbvCI     BclI
    BfrBI    BmgBI     BmtI   Bpu10I    BsiWI     BsmI    BspEI    BsrDI
    BsrGI   BstAPI    BstBI   BstEII  BstZ17I     ClaI    CspCI   DraIII
     DrdI     EciI    EcoRI     FalI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PmlI    PpuMI    PshAI     PsiI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     ScaI
    SexAI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     TatI  Tth111I     XbaI     XcmI     ZraI
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