Restriction map for pF1KB8492
##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AccIII      AclI        AflII       AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     AviII       AvrII       BamHI       BciVI       
BfaI        BfrI        BlnI        BlpI        Bpu1102I    
BsaWI       BseAI       BsiWI       BsmI        BspEI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
CelII       CpoI        DraI        EagI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HindIII     HpaI        
MaeI        MfeI        MluI        MroI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SapI        
SbfI        ScaI        SexAI       SfuI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
TfiI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I        628  GGTACC               G^GTACC
AcsI          915  GAATTT               [GA]^AATT[CT]
AocI         2397  CCTGAGG              CC^T[ACGT]AGG
ApoI          915  GAATTT               [GA]^AATT[CT]
Asp718I       628  GGTACC               G^GTACC
AspI         1720  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         1642  GGCGCC               GGCGC^C
BbvCI        1930  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1134  CGACGAGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1058  TGATCA               T^GATCA
BsaBI        1059  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        1724  CGGTCG               CG[GA][CT]^CG
Bsp1407I     1805  TGTACA               T^GTACA
BspLU11I       62  ACATGT               A^CATGT
BsrDI         438  CATTGC               GCAATG(2/0)
BsrGI        1805  TGTACA               T^GTACA
BssHII       1894  GCGCGC               G^CGCGC
BssSI        1414  CTCGTG               CACGAG(-5/-1)
Bsu36I       2397  CCTGAGG              CC^T[ACGT]AGG
DraIII       2072  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1720  GACCCGGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2397  CCTGAGG              CC^T[ACGT]AGG
FbaI         1058  TGATCA               T^GATCA
KasI         1642  GGCGCC               G^GCGCC
KpnI          628  GGTACC               GGTAC^C
KspI         2145  CCGCGG               CCGC^GG
MamI         1059  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI         1154  TTAA                 T^TAA
NarI         1642  GGCGCC               GG^CGCC
PflFI        1720  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1216  GACAAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          499  CTGCAG               CTGCA^G
SacII        2145  CCGCGG               CCGC^GG
SalI          325  GTCGAC               G^TCGAC
SfcI          499  CTGCAG               C^T[GA][CT]AG
SfiI         1521  GGCCGTGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1642  GGCGCC               GGC^GCC
SgrAI        1644  CGCCGGCG             C[GA]^CCGG[CT]G
SmlI         1882  CTCAAG               C^T[CT][GA]AG
SspBI        1805  TGTACA               T^GTACA
StuI         2354  AGGCCT               AGG^CCT
Tru9I        1154  TTAA                 T^TAA
Tth111I      1720  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AatII        2107  GACGTC               GACGT^C
             2116  GACGTC              
AccI           16  GTAGAC               GT^[AC][GT]AC
              325  GTCGAC              
AflIII         62  ACATGT               A^C[GA][CT]GT
             2306  ACGTGT              
AhdI          388  GACTGCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2395  GACCTGAGGTC         
AlwNI        1429  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2452  CAGATCCTG           
AspEI         388  GACTGCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2395  GACCTGAGGTC         
BalI          698  TGGCCA               TGG^CCA
             1580  TGGCCA              
BbrPI          79  CACGTG               CAC^GTG
             2305  CACGTG              
BbsI         1440  GAAGAC               GAAGAC(2/6)
             1246  GTCTTC              
BglII        1664  AGATCT               A^GATCT
             1943  AGATCT              
BpuAI        1440  GAAGAC               GAAGAC(2/6)
             1246  GTCTTC              
BspDI         613  ATCGAT               AT^CGAT
             1849  ATCGAT              
BspHI         608  TCATGA               T^CATGA
              911  TCATGA              
BsrBI         588  GAGCGG               CCGCTC(-3/-3)
              742  GAGCGG              
ClaI          613  ATCGAT               AT^CGAT
             1849  ATCGAT              
DdeI         1931  CTGAG                C^T[ACGT]AG
             2398  CTGAG               
Eam1105I      388  GACTGCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2395  GACCTGAGGTC         
EarI          229  CTCTTC               CTCTTC(1/4)
             1753  GAAGAG              
HaeII        1642  GGCGCC               [GA]GCGC^[CT]
             2177  GGCGCT              
Ksp632I       229  CTCTTC               CTCTTC(1/4)
             1753  GAAGAG              
MluNI         698  TGGCCA               TGG^CCA
             1580  TGGCCA              
MscI          698  TGGCCA               TGG^CCA
             1580  TGGCCA              
PflMI        1428  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2496  CCAGGTGGTGG         
PmaCI          79  CACGTG               CAC^GTG
             2305  CACGTG              
PmlI           79  CACGTG               CAC^GTG
             2305  CACGTG              
PpuMI        1319  AGGACCT              [GA]G^G[AT]CC[CT]
             1676  AGGACCT             
PvuII         502  CAGCTG               CAG^CTG
              871  CAGCTG              
RcaI          608  TCATGA               T^CATGA
              911  TCATGA              
SmaI          146  CCCGGG               CCC^GGG
              316  CCCGGG              
Tsp509I       916  AATT                 ^AATT
             2570  AATT                
Van91I       1428  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2496  CCAGGTGGTGG         
XmaCI         146  CCCGGG               C^CCGGG
              316  CCCGGG              
XmaI          146  CCCGGG               C^CCGGG
              316  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BglI        
BmyI        BpmI        BsaAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DpnII       DraII       DsaI        EaeI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PleI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XcmI        XhoII       
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