Restriction map for pF1KB5763
 (Linear) (Six-base) MAPSORT of: KIBB5763.seq  Check: 690  from: 1  to: 2448



KIBB5763.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     228    2448 
   Size:        228    2220
 
AccI GT'mk_AC

Cuts at:      0     398     715    2448 
   Size:        398     317    1733

  Fragments arranged by size: 

                1733     398     317
 
Acc65I G'GTAC_C

Cuts at:      0     677     835    2448 
   Size:        677     158    1613

  Fragments arranged by size: 

                1613     677     158
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     328     529     568    2448 
   Size:         34     294     201      39    1880

  Fragments arranged by size: 

                1880     294     201      39      34
 
AfeI AGC'GCT

Cuts at:      0     999    2448 
   Size:        999    1449
 
AflIII A'CryG_T

Cuts at:      0     471    2448 
   Size:        471    1977
 
AhdI GACnn_n'nnGTC

Cuts at:      0     735    2448 
   Size:        735    1713
 
AleI CACnn'nnGTG

Cuts at:      0    1753    2448 
   Size:       1753     695
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1015    1018    1265    2448 
   Size:       1015       3     247    1183

  Fragments arranged by size: 

                1183    1015     247       3
 
ApaI G_GGCC'C

Cuts at:      0      59    1651    1943    2448 
   Size:         59    1592     292     505

  Fragments arranged by size: 

                1592     505     292      59
 
ApaLI G'TGCA_C

Cuts at:      0     621    2448 
   Size:        621    1827
 
ApoI r'AATT_y

Cuts at:      0     385    2245    2448 
   Size:        385    1860     203

  Fragments arranged by size: 

                1860     385     203
 
AvaI C'yCGr_G

Cuts at:      0      52     688    1118    2448 
   Size:         52     636     430    1330

  Fragments arranged by size: 

                1330     636     430      52
 
BanI G'GyrC_C

Cuts at:      0     677     773     835     943    1405    2448 
   Size:        677      96      62     108     462    1043

  Fragments arranged by size: 

                1043     677     462     108      96      62
 
BanII G_rGCy'C

Cuts at:      0      50      59     587     695     995    1272    1651    1867 
   Size:         50       9     528     108     300     277     379     216

Cuts at:   1867    1943    2097    2448 
   Size:         76     154     351

  Fragments arranged by size: 

                 528     379     351     300     277     216     154     108
                  76      50       9
 
BbeI G_GCGC'C

Cuts at:      0     777    2448 
   Size:        777    1671
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      29    2096    2448 
   Size:         29    2067     352

  Fragments arranged by size: 

                2067     352      29
 
BglI GCCn_nnn'nGGC

Cuts at:      0      54     230    1144    2448 
   Size:         54     176     914    1304

  Fragments arranged by size: 

                1304     914     176      54
 
Bme1580I G_kGCm'C

Cuts at:      0      59     377     625     923    1080    1651    1943    2448 
   Size:         59     318     248     298     157     571     292     505

  Fragments arranged by size: 

                 571     505     318     298     292     248     157      59
            
 
BmgBI CAC'GTC

Cuts at:      0     150    2448 
   Size:        150    2298
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1253    1268    1789    1992    2448 
   Size:       1253      15     521     203     456

  Fragments arranged by size: 

                1253     521     456     203      15
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      54    2448 
   Size:         54    2394
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1516    2016    2260    2422    2448 
   Size:       1516     500     244     162      26

  Fragments arranged by size: 

                1516     500     244     162      26
 
Bpu10I CC'TnA_GC

Cuts at:      0     527    1505    2448 
   Size:        527     978     943

  Fragments arranged by size: 

                 978     943     527
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     571    1435    2448 
   Size:        301     270     864    1013

  Fragments arranged by size: 

                1013     864     301     270
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     169    2448 
   Size:        169    2279
 
BsaAI yAC'GTr

Cuts at:      0     402     474    2448 
   Size:        402      72    1974

  Fragments arranged by size: 

                1974     402      72
 
BsaHI Gr'CG_yC

Cuts at:      0     774    2084    2448 
   Size:        774    1310     364

  Fragments arranged by size: 

                1310     774     364
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     919     949    1797    1827    1917    1947    2448 
   Size:        919      30     848      30      90      30     501

  Fragments arranged by size: 

                 919     848     501      90      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     438    1476    1567    1805    2448 
   Size:        438    1038      91     238     643

  Fragments arranged by size: 

                1038     643     438     238      91
 
BseYI C'CCAG_C

Cuts at:      0     831     969     994    1483    1633    1740    1770    1832 
   Size:        831     138      25     489     150     107      30      62

Cuts at:   1832    1886    2141    2448 
   Size:         54     255     307

  Fragments arranged by size: 

                 831     489     307     255     150     138     107      62
                  54      30      25
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415    1316    1898    2448 
   Size:        415     901     582     550

  Fragments arranged by size: 

                 901     582     550     415
 
BsiEI CG_ry'CG

Cuts at:      0     951    2448 
   Size:        951    1497
 
BsiHKAI G_wGCw'C

Cuts at:      0     587     625     695    1682    2448 
   Size:        587      38      70     987     766

  Fragments arranged by size: 

                 987     766     587      70      38
 
Bsp1286I G_dGCh'C

Cuts at:      0      50      59     377     587     625     695     923     995 
   Size:         50       9     318     210      38      70     228      72

Cuts at:    995    1080    1272    1651    1682    1867    1943    2097    2448 
   Size:         85     192     379      31     185      76     154     351

  Fragments arranged by size: 

                 379     351     318     228     210     192     185     154
                  85      76      72      70      50      38      31       9
            
 
BspHI T'CATG_A

Cuts at:      0     650     821    2448 
   Size:        650     171    1627

  Fragments arranged by size: 

                1627     650     171
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     228     437    2435    2448 
   Size:        228     209    1998      13

  Fragments arranged by size: 

                1998     228     209      13
 
BsrBI CCG'CTC

Cuts at:      0     128    1548    1590    1611    2448 
   Size:        128    1420      42      21     837

  Fragments arranged by size: 

                1420     837     128      42      21
 
BsrFI r'CCGG_y

Cuts at:      0     229    1466    1518    1758    2448 
   Size:        229    1237      52     240     690

  Fragments arranged by size: 

                1237     690     240     229      52
 
BsrGI T'GTAC_A

Cuts at:      0     801    2448 
   Size:        801    1647
 
BssSI C'ACGA_G

Cuts at:      0     611    2448 
   Size:        611    1837
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1018    2448 
   Size:       1018    1430
 
BstEII G'GTnAC_C

Cuts at:      0     276    2448 
   Size:        276    2172
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     108     564    1840    1927    1989    2448 
   Size:        108     456    1276      87      62     459

  Fragments arranged by size: 

                1276     459     456     108      87      62
 
BstYI r'GATC_y

Cuts at:      0     254     311    1193    2393    2448 
   Size:        254      57     882    1200      55

  Fragments arranged by size: 

                1200     882     254      57      55
 
BstZ17I GTA'TAC

Cuts at:      0     716    2448 
   Size:        716    1732
 
Bsu36I CC'TnA_GG

Cuts at:      0     298     361    2448 
   Size:        298      63    2087

  Fragments arranged by size: 

                2087     298      63
 
BtgI C'CryG_G

Cuts at:      0    1289    2352    2448 
   Size:       1289    1063      96

  Fragments arranged by size: 

                1289    1063      96
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     327    2448 
   Size:        327    2121
 
BtsI GCAGTG_nn'

Cuts at:      0     964    2214    2448 
   Size:        964    1250     234

  Fragments arranged by size: 

                1250     964     234
 
DraI TTT'AAA

Cuts at:      0     327    2448 
   Size:        327    2121
 
EaeI y'GGCC_r

Cuts at:      0       2      98     231     242     665    1040    2295    2448 
   Size:          2      96     133      11     423     375    1255     153

  Fragments arranged by size: 

                1255     423     375     153     133      96      11       2
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     787    2448 
   Size:        787    1661
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     201    1174    1578    2448 
   Size:        201     973     404     870

  Fragments arranged by size: 

                 973     870     404     201
 
EcoICRI GAG'CTC

Cuts at:      0     585     693    2448 
   Size:        585     108    1755

  Fragments arranged by size: 

                1755     585     108
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     328     529     568    1516    2016    2260    2422 
   Size:         34     294     201      39     948     500     244     162

Cuts at:   2422    2448 
   Size:         26

  Fragments arranged by size: 

                 948     500     294     244     201     162      39      34
                  26
 
EcoO109I rG'GnC_Cy

Cuts at:      0     191     504     957    1637    1647    1648    2169    2442 
   Size:        191     313     453     680      10       1     521     273

Cuts at:   2442    2448 
   Size:          6

  Fragments arranged by size: 

                 680     521     453     313     273     191      10       6
                   1
 
EcoRI G'AATT_C

Cuts at:      0     385    2245    2448 
   Size:        385    1860     203

  Fragments arranged by size: 

                1860     385     203
 
HaeII r_GCGC'y

Cuts at:      0     777    1001    1539    1777    2448 
   Size:        777     224     538     238     671

  Fragments arranged by size: 

                 777     671     538     238     224
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     721     753    1664    1696    2296    2328    2362    2394 
   Size:        721      32     911      32     600      32      34      32

Cuts at:   2394    2448 
   Size:         54

  Fragments arranged by size: 

                 911     721     600      54      34      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    2130    2448 
   Size:       2130     318
 
KasI G'GCGC_C

Cuts at:      0     773    2448 
   Size:        773    1675
 
KpnI G_GTAC'C

Cuts at:      0     681     839    2448 
   Size:        681     158    1609

  Fragments arranged by size: 

                1609     681     158
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     581    1685    1824    2448 
   Size:        581    1104     139     624

  Fragments arranged by size: 

                1104     624     581     139
 
MscI TGG'CCA

Cuts at:      0     244     667    1042    2297    2448 
   Size:        244     423     375    1255     151

  Fragments arranged by size: 

                1255     423     375     244     151
 
MslI CAynn'nnrTG

Cuts at:      0     823     826    1753    2272    2448 
   Size:        823       3     927     519     176

  Fragments arranged by size: 

                 927     823     519     176       3
 
MspA1I CmG'CkG

Cuts at:      0      85     634    1015    1542    2448 
   Size:         85     549     381     527     906

  Fragments arranged by size: 

                 906     549     527     381      85
 
NaeI GCC'GGC

Cuts at:      0    1468    1760    2448 
   Size:       1468     292     688

  Fragments arranged by size: 

                1468     688     292
 
NarI GG'CG_CC

Cuts at:      0     774    2448 
   Size:        774    1674
 
NcoI C'CATG_G

Cuts at:      0    1289    2352    2448 
   Size:       1289    1063      96

  Fragments arranged by size: 

                1289    1063      96
 
NgoMIV G'CCGG_C

Cuts at:      0    1466    1758    2448 
   Size:       1466     292     690

  Fragments arranged by size: 

                1466     690     292
 
NspI r_CATG'y

Cuts at:      0     519     981    2448 
   Size:        519     462    1467

  Fragments arranged by size: 

                1467     519     462
 
PasI CC'CwG_GG

Cuts at:      0     923    1265    2017    2448 
   Size:        923     342     752     431

  Fragments arranged by size: 

                 923     752     431     342
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     240     674    1265    1906    2017    2293    2448 
   Size:        240     434     591     641     111     276     155

  Fragments arranged by size: 

                 641     591     434     276     240     155     111
 
PfoI T'CCnGG_A

Cuts at:      0     407    2438    2448 
   Size:        407    2031      10

  Fragments arranged by size: 

                2031     407      10
 
PmlI CAC'GTG

Cuts at:      0     474    2448 
   Size:        474    1974
 
PpuMI rG'GwC_Cy

Cuts at:      0    2169    2442    2448 
   Size:       2169     273       6

  Fragments arranged by size: 

                2169     273       6
 
PspOMI G'GGCC_C

Cuts at:      0      55    1647    1939    2448 
   Size:         55    1592     292     509

  Fragments arranged by size: 

                1592     509     292      55
 
PstI C_TGCA'G

Cuts at:      0    1361    2387    2448 
   Size:       1361    1026      61

  Fragments arranged by size: 

                1361    1026      61
 
PvuII CAG'CTG

Cuts at:      0    1015    2448 
   Size:       1015    1433
 
SacI G_AGCT'C

Cuts at:      0     587     695    2448 
   Size:        587     108    1753

  Fragments arranged by size: 

                1753     587     108
 
SapI GCTCTTCn'nnn_

Cuts at:      0     787    2448 
   Size:        787    1661
 
ScaI AGT'ACT

Cuts at:      0     646    2448 
   Size:        646    1802
 
SfcI C'TryA_G

Cuts at:      0      92     811    1213    1357    1826    2383    2448 
   Size:         92     719     402     144     469     557      65

  Fragments arranged by size: 

                 719     557     469     402     144      92      65
 
SfoI GGC'GCC

Cuts at:      0     775    2448 
   Size:        775    1673
 
SmaI CCC'GGG

Cuts at:      0      54    2448 
   Size:         54    2394
 
SmlI C'TyrA_G

Cuts at:      0     316     586    1450    2448 
   Size:        316     270     864     998

  Fragments arranged by size: 

                 998     864     316     270
 
SpeI A'CTAG_T

Cuts at:      0    1674    2448 
   Size:       1674     774
 
SphI G_CATG'C

Cuts at:      0     981    2448 
   Size:        981    1467
 
StyI C'CwwG_G

Cuts at:      0    1289    1583    1664    2352    2448 
   Size:       1289     294      81     688      96

  Fragments arranged by size: 

                1289     688     294      96      81
 
TatI w'GTAC_w

Cuts at:      0     512     644     801    2448 
   Size:        512     132     157    1647

  Fragments arranged by size: 

                1647     512     157     132
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1917    1949    2448 
   Size:       1917      32     499

  Fragments arranged by size: 

                1917     499      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1491    1662    1986    2448 
   Size:       1491     171     324     462

  Fragments arranged by size: 

                1491     462     324     171
 
XmaI C'CCGG_G

Cuts at:      0      52    2448 
   Size:         52    2396
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI     AleI
    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI
     BbsI     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI
    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     DraI     EaeI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    HaeII    Hin4I   HincII     KasI     KpnI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII
     SacI     SapI     ScaI     SfcI     SfoI     SmaI     SmlI     SpeI
     SphI     StyI     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AgeI     AlfI     AloI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI     BmtI    BsaBI    BsaWI    BsiWI     BsmI
    BsmBI    BspEI    BsrDI   BssHII    BstBI     ClaI    CspCI   DraIII
     DrdI     EagI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XbaI     XhoI     XmnI     ZraI
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