Restriction map for pF1KB5709
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AvrII       BbeI        
BbrPI       BcgI        BciVI       BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsiWI       BsmBI       BsmI        
BspHI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     Bsu36I      CelII       
Cfr10I      CpoI        DraI        DraIII      Eam1105I    
Eco81I      EcoRI       EcoRV       EcoT22I     FseI        
Hin1I       HindIII     HpaI        KasI        KpnI        
MaeII       MamI        MfeI        MluI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NruI        NsiI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        StuI        SwaI        XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII        1425  CGCG                 CG^CG
AccIII        515  TCCGGA               T^CCGGA
AflIII        266  ACATGT               A^C[GA][CT]GT
AlwNI        2183  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1826  AGCGCT               AGC^GCT
AspI         1635  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1259  CTCGAG               C^[CT]CG[GA]G
AviII        1445  TGCGCA               TGC^GCA
BamHI         743  GGATCC               G^GATCC
BanI          453  GGTGCC               G^G[CT][GA]CC
BclI         1070  TGATCA               T^GATCA
BcnI         1523  CCCGG                CC^[GC]GG
BglII        1004  AGATCT               A^GATCT
BseAI         515  TCCGGA               T^CCGGA
BsiEI        2268  CGGCCG               CG[GA][CT]^CG
BsoBI        1259  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      754  TGTACA               T^GTACA
BspDI        2227  ATCGAT               AT^CGAT
BspEI         515  TCCGGA               T^CCGGA
BspLU11I      266  ACATGT               A^CATGT
BsrGI         754  TGTACA               T^GTACA
BstAPI       1566  GCAGATGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2122  GGTAACC              G^GT[ACGT]ACC
BstPI        2122  GGTAACC              G^GT[ACGT]ACC
BstUI        1425  CGCG                 CG^CG
ClaI         2227  ATCGAT               AT^CGAT
EagI         2268  CGGCCG               C^GGCCG
EclXI        2268  CGGCCG               C^GGCCG
Eco47III     1826  AGCGCT               AGC^GCT
Eco52I       2268  CGGCCG               C^GGCCG
EcoO65I      2122  GGTAACC              G^GT[ACGT]ACC
FbaI         1070  TGATCA               T^GATCA
FspI         1445  TGCGCA               TGC^GCA
HaeII        1826  AGCGCT               [GA]GCGC^[CT]
HgaI          988  GACGC                GACGC(5/10)
KspI         1424  CCGCGG               CCGC^GG
MroI          515  TCCGGA               T^CCGGA
MvnI         1425  CGCG                 CG^CG
NciI         1523  CCCGG                CC^[GC]GG
NotI         2267  GCGGCCGC             GC^GGCCGC
PaeR7I       1259  CTCGAG               C^TCGAG
PflFI        1635  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacII        1424  CCGCGG               CCGC^GG
SphI         1844  GCATGC               GCATG^C
SspBI         754  TGTACA               T^GTACA
Tth111I      1635  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1268  CCAACGGGGAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1259  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI          755  GTAC                 GT^AC
             1980  GTAC                
Asp700I       501  GAATAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2028  GAATCCATTC          
BalI         1818  TGGCCA               TGG^CCA
             2327  TGGCCA              
BfaI          335  CTAG                 C^TAG
             1723  CTAG                
BsaWI         515  TCCGGA               [AT]^CCGG[AT]
              740  ACCGGA              
BsmAI         919  GAGAC                GTCTC(1/5)
             1200  GAGAC               
BspMI        1463  ACCTGC               ACCTGC(4/8)
             1547  ACCTGC              
BsrBI        1372  CCGCTC               CCGCTC(-3/-3)
             1262  GAGCGG              
BstXI         279  CCAGGAGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1901  CCAGCCCCATGG        
CfoI         1446  GCGC                 GCG^C
             1827  GCGC                
DrdI          121  GACCGTGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              469  GACCAGGATGTC        
EcoNI        1933  CCTGCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2274  CCTGGATGAGG         
HhaI         1446  GCGC                 GCG^C
             1827  GCGC                
HinP1I       1446  GCGC                 G^CGC
             1827  GCGC                
HincII        463  GTCAAC               GT[CT]^[GA]AC
              865  GTTGAC              
HindII        463  GTCAAC               GT[CT]^[GA]AC
              865  GTTGAC              
HphI          605  TCACC                GGTGA(8/7)
              655  TCACC               
MaeI          335  CTAG                 C^TAG
             1723  CTAG                
MluNI        1818  TGGCCA               TGG^CCA
             2327  TGGCCA              
MscI         1818  TGGCCA               TGG^CCA
             2327  TGGCCA              
NcoI          669  CCATGG               C^CATGG
             1907  CCATGG              
RsaI          755  GTAC                 GT^AC
             1980  GTAC                
SacI          574  GAGCTC               GAGCT^C
             2209  GAGCTC              
SapI         2206  GAAGAGC              GCTCTTC(1/4)
             1727  GCTCTTC             
XmnI          501  GAATAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2028  GAATCCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AluI        AlwI        ApaI        AspHI       
AvaII       BanII       BbsI        BbvCI       BbvI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
Ksp632I     MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       
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