Restriction map for pF1KB5709
 (Linear) (Six-base) MAPSORT of: KIBB5709.seq  Check: 288  from: 1  to: 2352



KIBB5709.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79    1635    1943    2052    2335    2352 
   Size:         79    1556     308     109     283      17

  Fragments arranged by size: 

                1556     308     283     109      79      17
 
AfeI AGC'GCT

Cuts at:      0    1828    2352 
   Size:       1828     524
 
AflIII A'CryG_T

Cuts at:      0     266    2352 
   Size:        266    2086
 
AleI CACnn'nnGTG

Cuts at:      0    1792    2352 
   Size:       1792     560
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2188    2352 
   Size:       2188     164
 
ApaI G_GGCC'C

Cuts at:      0    1300    1808    1868    2352 
   Size:       1300     508      60     484

  Fragments arranged by size: 

                1300     508     484      60
 
AvaI C'yCGr_G

Cuts at:      0    1259    2352 
   Size:       1259    1093
 
BamHI G'GATC_C

Cuts at:      0     743    2352 
   Size:        743    1609
 
BanI G'GyrC_C

Cuts at:      0     453    2352 
   Size:        453    1899
 
BanII G_rGCy'C

Cuts at:      0     578    1300    1682    1808    1868    2198    2213    2352 
   Size:        578     722     382     126      60     330      15     139

  Fragments arranged by size: 

                 722     578     382     330     139     126      60      15
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     125    1898    2084    2352 
   Size:        125    1773     186     268

  Fragments arranged by size: 

                1773     268     186     125
 
BbvCI CC'TCA_GC

Cuts at:      0    1319    1535    2189    2352 
   Size:       1319     216     654     163

  Fragments arranged by size: 

                1319     654     216     163
 
BclI T'GATC_A

Cuts at:      0    1070    2352 
   Size:       1070    1282
 
BglII A'GATC_T

Cuts at:      0    1004    2352 
   Size:       1004    1348
 
Bme1580I G_kGCm'C

Cuts at:      0    1300    1808    1868    2347    2352 
   Size:       1300     508      60     479       5

  Fragments arranged by size: 

                1300     508     479      60       5
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1729    2352 
   Size:       1729     623
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1884    2352 
   Size:       1884     468
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     863    1234    1363    1696    1884    2352 
   Size:        863     371     129     333     188     468

  Fragments arranged by size: 

                 863     468     371     333     188     129
 
Bpu10I CC'TnA_GC

Cuts at:      0     628    1319    1535    2189    2352 
   Size:        628     691     216     654     163

  Fragments arranged by size: 

                 691     654     628     216     163
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     763    1252    1329    2088    2352 
   Size:        763     489      77     759     264

  Fragments arranged by size: 

                 763     759     489     264      77
 
BsaWI w'CCGG_w

Cuts at:      0     515     740    2352 
   Size:        515     225    1612

  Fragments arranged by size: 

                1612     515     225
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1914    1944    2352 
   Size:       1914      30     408

  Fragments arranged by size: 

                1914     408      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1366    1552    1555    2020    2023    2203    2206    2352 
   Size:       1366     186       3     465       3     180       3     146

  Fragments arranged by size: 

                1366     465     186     180     146       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     646     913    1220    1681    1809    2052    2090    2352 
   Size:        646     267     307     461     128     243      38     262

  Fragments arranged by size: 

                 646     461     307     267     262     243     128      38
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     985    1117    1609    2352 
   Size:        985     132     492     743

  Fragments arranged by size: 

                 985     743     492     132
 
BsiEI CG_ry'CG

Cuts at:      0    2271    2352 
   Size:       2271      81
 
BsiHKAI G_wGCw'C

Cuts at:      0     578     634     812    2213    2352 
   Size:        578      56     178    1401     139

  Fragments arranged by size: 

                1401     578     178     139      56
 
Bsp1286I G_dGCh'C

Cuts at:      0     578     634     812    1300    1682    1808    1868    2198 
   Size:        578      56     178     488     382     126      60     330

Cuts at:   2198    2213    2347    2352 
   Size:         15     134       5

  Fragments arranged by size: 

                 578     488     382     330     178     134     126      60
                  56      15       5
 
BspEI T'CCGG_A

Cuts at:      0     515    2352 
   Size:        515    1837
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1472    1556    2352 
   Size:       1472      84     796

  Fragments arranged by size: 

                1472     796      84
 
BsrBI CCG'CTC

Cuts at:      0    1264    1374    2352 
   Size:       1264     110     978

  Fragments arranged by size: 

                1264     978     110
 
BsrGI T'GTAC_A

Cuts at:      0     754    2352 
   Size:        754    1598
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1572    2352 
   Size:       1572     780
 
BstEII G'GTnAC_C

Cuts at:      0    2122    2352 
   Size:       2122     230
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     286    1908    2352 
   Size:        286    1622     444

  Fragments arranged by size: 

                1622     444     286
 
BstYI r'GATC_y

Cuts at:      0      63     743    1004    1385    1719    2352 
   Size:         63     680     261     381     334     633

  Fragments arranged by size: 

                 680     633     381     334     261      63
 
BtgI C'CryG_G

Cuts at:      0     669    1193    1424    1907    2352 
   Size:        669     524     231     483     445

  Fragments arranged by size: 

                 669     524     483     445     231
 
BtsI GCAGTG_nn'

Cuts at:      0     392     531     711     759    1123    2024    2269    2352 
   Size:        392     139     180      48     364     901     245      83

  Fragments arranged by size: 

                 901     392     364     245     180     139      83      48
            
 
ClaI AT'CG_AT

Cuts at:      0    2228    2352 
   Size:       2228     124
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     127     475    2352 
   Size:        127     348    1877

  Fragments arranged by size: 

                1877     348     127
 
EaeI y'GGCC_r

Cuts at:      0    1265    1818    2268    2327    2352 
   Size:       1265     553     450      59      25

  Fragments arranged by size: 

                1265     553     450      59      25
 
EagI C'GGCC_G

Cuts at:      0    2268    2352 
   Size:       2268      84
 
EarI CTCTTCn'nnn_

Cuts at:      0    1180    1734    1907    1991    2201    2352 
   Size:       1180     554     173      84     210     151

  Fragments arranged by size: 

                1180     554     210     173     151      84
 
EcoICRI GAG'CTC

Cuts at:      0     576    2211    2352 
   Size:        576    1635     141

  Fragments arranged by size: 

                1635     576     141
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     863    1234    1363    1635    1696    1884    1943 
   Size:         79     784     371     129     272      61     188      59

Cuts at:   1943    2052    2335    2352 
   Size:        109     283      17

  Fragments arranged by size: 

                 784     371     283     272     188     129     109      79
                  61      59      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1937    2278    2352 
   Size:       1937     341      74

  Fragments arranged by size: 

                1937     341      74
 
EcoO109I rG'GnC_Cy

Cuts at:      0     221    1029    1296    1297    1545    1805    1864    1865 
   Size:        221     808     267       1     248     260      59       1

Cuts at:   1865    2352 
   Size:        487

  Fragments arranged by size: 

                 808     487     267     260     248     221      59       1
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1023    2352 
   Size:       1023    1329
 
FspI TGC'GCA

Cuts at:      0    1447    2352 
   Size:       1447     905
 
FspAI rTGC'GCAy

Cuts at:      0    1447    2352 
   Size:       1447     905
 
HaeII r_GCGC'y

Cuts at:      0    1830    2352 
   Size:       1830     522
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     288     320     674     706    1267    1299 
   Size:         55      32     201      32     354      32     561      32

Cuts at:   1299    1711    1743    2286    2318    2352 
   Size:        412      32     543      32      34

  Fragments arranged by size: 

                 561     543     412     354     201      55      34      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     465     867    2352 
   Size:        465     402    1485

  Fragments arranged by size: 

                1485     465     402
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     335    1568    2352 
   Size:        335    1233     784

  Fragments arranged by size: 

                1233     784     335
 
MscI TGG'CCA

Cuts at:      0    1820    2329    2352 
   Size:       1820     509      23

  Fragments arranged by size: 

                1820     509      23
 
MslI CAynn'nnrTG

Cuts at:      0     892     944    1792    2352 
   Size:        892      52     848     560

  Fragments arranged by size: 

                 892     848     560      52
 
MspA1I CmG'CkG

Cuts at:      0     749    1426    1473    1554    1563    1674    2352 
   Size:        749     677      47      81       9     111     678

  Fragments arranged by size: 

                 749     678     677     111      81      47       9
 
NcoI C'CATG_G

Cuts at:      0     669    1907    2352 
   Size:        669    1238     445

  Fragments arranged by size: 

                1238     669     445
 
NotI GC'GGCC_GC

Cuts at:      0    2268    2352 
   Size:       2268      84
 
NspI r_CATG'y

Cuts at:      0     270     943    1848    2352 
   Size:        270     673     905     504

  Fragments arranged by size: 

                 905     673     504     270
 
PasI CC'CwG_GG

Cuts at:      0      19    2352 
   Size:         19    2333
 
PciI A'CATG_T

Cuts at:      0     266    2352 
   Size:        266    2086
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     496    1796    1816    2352 
   Size:        496    1300      20     536

  Fragments arranged by size: 

                1300     536     496      20
 
PfoI T'CCnGG_A

Cuts at:      0     719    1522    2352 
   Size:        719     803     830

  Fragments arranged by size: 

                 830     803     719
 
PpuMI rG'GwC_Cy

Cuts at:      0     221    1029    1545    2352 
   Size:        221     808     516     807

  Fragments arranged by size: 

                 808     807     516     221
 
PspOMI G'GGCC_C

Cuts at:      0    1296    1804    1864    2352 
   Size:       1296     508      60     488

  Fragments arranged by size: 

                1296     508     488      60
 
PstI C_TGCA'G

Cuts at:      0      71    1064    1457    1478    1553    1568    1941    2222 
   Size:         71     993     393      21      75      15     373     281

Cuts at:   2222    2352 
   Size:        130

  Fragments arranged by size: 

                 993     393     373     281     130      75      71      21
                  15
 
PvuII CAG'CTG

Cuts at:      0    1473    1563    1674    2352 
   Size:       1473      90     111     678

  Fragments arranged by size: 

                1473     678     111      90
 
SacI G_AGCT'C

Cuts at:      0     578    2213    2352 
   Size:        578    1635     139

  Fragments arranged by size: 

                1635     578     139
 
SacII CC_GC'GG

Cuts at:      0    1427    2352 
   Size:       1427     925
 
SanDI GG'GwC_CC

Cuts at:      0     221    2352 
   Size:        221    2131
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1734    2201    2352 
   Size:       1734     467     151

  Fragments arranged by size: 

                1734     467     151
 
SfcI C'TryA_G

Cuts at:      0      67     927    1060    1453    1474    1549    1564    1937 
   Size:         67     860     133     393      21      75      15     373

Cuts at:   1937    2218    2352 
   Size:        281     134

  Fragments arranged by size: 

                 860     393     373     281     134     133      75      67
                  21      15
 
SmlI C'TyrA_G

Cuts at:      0     778    1231    1259    1308    2103    2352 
   Size:        778     453      28      49     795     249

  Fragments arranged by size: 

                 795     778     453     249      49      28
 
SphI G_CATG'C

Cuts at:      0    1848    2352 
   Size:       1848     504
 
StyI C'CwwG_G

Cuts at:      0     225     457     590     669    1013    1418    1907    2352 
   Size:        225     232     133      79     344     405     489     445

  Fragments arranged by size: 

                 489     445     405     344     232     225     133      79
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     649     672     705    1628    1714    2352 
   Size:        649      23      33     923      86     638

  Fragments arranged by size: 

                 923     649     638      86      33      23
 
TatI w'GTAC_w

Cuts at:      0     754    1979    2352 
   Size:        754    1225     373

  Fragments arranged by size: 

                1225     754     373
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1911    1943    2352 
   Size:       1911      32     409

  Fragments arranged by size: 

                1911     409      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1638    2352 
   Size:       1638     714
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1275    2352 
   Size:       1275    1077
 
XhoI C'TCGA_G

Cuts at:      0    1259    2352 
   Size:       1259    1093
 
XmnI GAAnn'nnTTC

Cuts at:      0     505    2032    2352 
   Size:        505    1527     320

  Fragments arranged by size: 

                1527     505     320
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AleI    AlwNI     ApaI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI     BclI    BglII Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrGI
   BstAPI   BstEII    BstXI    BstYI     BtgI     BtsI     ClaI     DrdI
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FspI    FspAI    HaeII    Hin4I   HincII     MmeI     MscI     MslI
   MspA1I     NcoI     NotI     NspI     PasI     PciI    PflMI     PfoI
    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI     SapI
     SfcI     SmlI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BciVI    BfrBI     BglI     BlpI    BmgBI
     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsiWI     BsmI    BsmBI
    BspHI    BsrDI    BsrFI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I
    BtgZI    CspCI     DraI   DraIII     EciI    EcoRI    EcoRV     FseI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NruI     NsiI     PacI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     XbaI     XmaI
     ZraI
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