Restriction map for pF1KB8515
##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

Acc65I      AclI        AgeI        AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvrII       BciVI       BclI        
BglII       BlnI        BsiWI       BsmI        Bsp1407I    
BspLU11I    BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     DraI        Eam1105I    EcoRV       
EcoT22I     FbaI        HindIII     HpaI        KpnI        
MfeI        MluI        MunI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        SalI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI         2227  GTCTAC               GT^[AC][GT]AC
AccIII       1574  TCCGGA               T^CCGGA
AflII        1201  CTTAAG               C^TTAAG
AocI         2368  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       736  AGCGCT               AGC^GCT
AviII         656  TGCGCA               TGC^GCA
BalI          422  TGGCCA               TGG^CCA
BbeI         2672  GGCGCC               GGCGC^C
BbsI         2485  GTCTTC               GAAGAC(2/6)
BbvCI        1432  GCTGAGG              CCTCAGC(-5/-2)
BcgI         2601  CGAGAGAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         1201  CTTAAG               C^TTAAG
BlpI          732  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      732  GCTCAGC              GC^T[ACGT]AGC
BpuAI        2485  GTCTTC               GAAGAC(2/6)
BseAI        1574  TCCGGA               T^CCGGA
BsmBI        1979  GAGACG               CGTCTC(1/5)
BspDI        1546  ATCGAT               AT^CGAT
BspEI        1574  TCCGGA               T^CCGGA
BspHI        2552  TCATGA               T^CATGA
BsrDI         476  GCAATG               GCAATG(2/0)
BstEII       1437  GGTCACC              G^GT[ACGT]ACC
BstPI        1437  GGTCACC              G^GT[ACGT]ACC
Bsu36I       2368  CCTGAGG              CC^T[ACGT]AGG
CelII         732  GCTCAGC              GC^T[ACGT]AGC
ClaI         1546  ATCGAT               AT^CGAT
CpoI          752  CGGACCG              CG^G[AT]CCG
Eco47III      736  AGCGCT               AGC^GCT
Eco81I       2368  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1437  GGTCACC              G^GT[ACGT]ACC
EcoRI        1915  GAATTC               G^AATTC
FseI          585  GGCCGGCC             GGCCGG^CC
FspI          656  TGCGCA               TGC^GCA
HgaI         2776  GACGC                GACGC(5/10)
HincII       2500  GTCAAC               GT[CT]^[GA]AC
HindII       2500  GTCAAC               GT[CT]^[GA]AC
KasI         2672  GGCGCC               G^GCGCC
KspI         2795  CCGCGG               CCGC^GG
MluNI         422  TGGCCA               TGG^CCA
MroI         1574  TCCGGA               T^CCGGA
MscI          422  TGGCCA               TGG^CCA
MseI         1202  TTAA                 T^TAA
NaeI          586  GCCGGC               GCC^GGC
NarI         2672  GGCGCC               GG^CGCC
NgoMIV        586  GCCGGC               G^CCGGC
NspI          560  GCATGC               [GA]CATG^[CT]
RcaI         2552  TCATGA               T^CATGA
RsrII         752  CGGACCG              CG^G[AT]CCG
SacII        2795  CCGCGG               CCGC^GG
ScaI         2105  AGTACT               AGT^ACT
SfoI         2672  GGCGCC               GGC^GCC
SmlI         1201  CTTAAG               C^T[CT][GA]AG
SphI          560  GCATGC               GCATG^C
Tru9I        1202  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AatII        1230  GACGTC               GACGT^C
             2716  GACGTC              
ApaI          228  GGGCCC               GGGCC^C
              934  GGGCCC              
BamHI        1633  GGATCC               G^GATCC
             1722  GGATCC              
BsaBI         546  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1539  GATGAACATC          
BsrFI         586  GCCGGC               [GA]^CCGG[CT]
             2113  GCCGGT              
BstXI         206  CCACTGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              348  CCAGTCCCCTGG        
Cfr10I        586  GCCGGC               [GA]^CCGG[CT]
             2113  GCCGGT              
DrdI         2379  GACGGACACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2491  GACAGCATCGTC        
EcoNI        2298  CCTCTGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2633  CCTTCTTCAGG         
MamI          546  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1539  GATGAACATC          
SmaI          103  CCCGGG               CCC^GGG
             1112  CCCGGG              
XcmI          203  CCACCACTGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              797  CCAACCAGAAGCTGG     
XmaCI         103  CCCGGG               C^CCGGG
             1112  CCCGGG              
XmaI          103  CCCGGG               C^CCGGG
             1112  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
Asp700I     AspHI       AvaI        AvaII       BanI        
BanII       BbrPI       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PmaCI       PmlI        PpuMI       PstI        
PvuII       RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmnI        
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