Restriction map for
pF1KB9993
##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BbrPI BbsI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaI BsiEI BsiWI Bsp1407I BspDI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI KspI MamI MfeI
MluI MseI MslI MunI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AcsI 676 AAATTT [GA]^AATT[CT]
AcyI 227 GGCGCC G[GA]^CG[CT]C
Alw44I 1361 GTGCAC G^TGCAC
AlwNI 1121 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1361 GTGCAC G^TGCAC
ApoI 676 AAATTT [GA]^AATT[CT]
Asp700I 1386 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 457 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 798 GGATCC G^GATCC
BanI 227 GGCGCC G^G[CT][GA]CC
BbeI 227 GGCGCC GGCGC^C
BbvCI 1495 GCTGAGG CCTCAGC(-5/-2)
BcgI 879 CGAAGGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1300 CTAG C^TAG
BglI 211 GCCAACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 227 GGCGCC G[GA]^CG[CT]C
BsgI 1139 CTGCAC GTGCAG(16/14)
BsmBI 705 CGTCTC CGTCTC(1/5)
BsmI 686 GAATGC GAATGC(1/-1)
BspHI 975 TCATGA T^CATGA
BsrDI 1178 CATTGC GCAATG(2/0)
BsrFI 1484 GCCGGC [GA]^CCGG[CT]
BstAPI 1141 GCACCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1393 TTCGAA TT^CGAA
Cfr10I 1484 GCCGGC [GA]^CCGG[CT]
HaeII 227 GGCGCC [GA]GCGC^[CT]
Hin1I 227 GGCGCC G[GA]^CG[CT]C
HincII 1285 GTCAAC GT[CT]^[GA]AC
HindII 1285 GTCAAC GT[CT]^[GA]AC
KasI 227 GGCGCC G^GCGCC
MaeI 1300 CTAG C^TAG
MaeII 1289 ACGT A^CGT
MspA1I 260 CAGCTG C[AC]G^C[GT]G
NaeI 1484 GCCGGC GCC^GGC
NarI 227 GGCGCC GG^CGCC
NgoMIV 1484 GCCGGC G^CCGGC
NspI 767 ACATGC [GA]CATG^[CT]
NspV 1393 TTCGAA TT^CGAA
PflFI 457 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 861 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1507 CTGCAG CTGCA^G
PvuII 260 CAGCTG CAG^CTG
RcaI 975 TCATGA T^CATGA
ScaI 1043 AGTACT AGT^ACT
SfoI 227 GGCGCC GGC^GCC
SfuI 1393 TTCGAA TT^CGAA
StuI 917 AGGCCT AGG^CCT
TfiI 846 GAATC G^A[AT]TC
Tsp509I 677 AATT ^AATT
Tth111I 457 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 861 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1386 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1090 GTAGAC GT^[AC][GT]AC
1724 GTCTAC
AccII 434 CGCG CG^CG
1408 CGCG
AccIII 38 TCCGGA T^CCGGA
801 TCCGGA
ApaI 127 GGGCCC GGGCC^C
1333 GGGCCC
BsaWI 38 TCCGGA [AT]^CCGG[AT]
801 TCCGGA
BseAI 38 TCCGGA T^CCGGA
801 TCCGGA
BsmAI 48 GTCTC GTCTC(1/5)
706 GTCTC
BspEI 38 TCCGGA T^CCGGA
801 TCCGGA
BstEII 558 GGTCACC G^GT[ACGT]ACC
966 GGTCACC
BstPI 558 GGTCACC G^GT[ACGT]ACC
966 GGTCACC
BstUI 434 CGCG CG^CG
1408 CGCG
BstXI 572 CCATCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1160 CCAGCCACTTGG
EarI 1403 CTCTTC CTCTTC(1/4)
1024 GAAGAG
EcoNI 318 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1299 CCTAGACAAGG
EcoO65I 558 GGTCACC G^GT[ACGT]ACC
966 GGTCACC
Ksp632I 1403 CTCTTC CTCTTC(1/4)
1024 GAAGAG
MroI 38 TCCGGA T^CCGGA
801 TCCGGA
MvnI 434 CGCG CG^CG
1408 CGCG
NcoI 1436 CCATGG C^CATGG
1470 CCATGG
PshAI 154 GACGATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
552 GACTCAGGTC
SapI 1024 GAAGAGC GCTCTTC(1/4)
1402 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MluNI
MnlI MscI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII
XmaCI XmaI