Restriction map for
pF1KB9993
(Linear) (Six-base) MAPSORT of: KIBB9993.seq Check: 1509 from: 1 to: 1752
KIBB9993.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 0 1091 1725 1752
Size: 1091 634 27
Fragments arranged by size:
1091 634 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1243 1752
Size: 1243 509
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1450 1482 1752
Size: 1450 32 270
Fragments arranged by size:
1450 270 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1126 1752
Size: 1126 626
ApaI G_GGCC'C
Cuts at: 0 131 1337 1752
Size: 131 1206 415
Fragments arranged by size:
1206 415 131
ApaLI G'TGCA_C
Cuts at: 0 1361 1752
Size: 1361 391
ApoI r'AATT_y
Cuts at: 0 676 1752
Size: 676 1076
AvaI C'yCGr_G
Cuts at: 0 365 472 1059 1367 1752
Size: 365 107 587 308 385
Fragments arranged by size:
587 385 365 308 107
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 486 519 1752
Size: 486 33 1233
Fragments arranged by size:
1233 486 33
BamHI G'GATC_C
Cuts at: 0 798 1752
Size: 798 954
BanI G'GyrC_C
Cuts at: 0 227 1752
Size: 227 1525
BanII G_rGCy'C
Cuts at: 0 131 1337 1463 1752
Size: 131 1206 126 289
Fragments arranged by size:
1206 289 131 126
BbeI G_GCGC'C
Cuts at: 0 231 1752
Size: 231 1521
BbvCI CC'TCA_GC
Cuts at: 0 1496 1752
Size: 1496 256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 868 902 1752
Size: 868 34 850
Fragments arranged by size:
868 850 34
BglI GCCn_nnn'nGGC
Cuts at: 0 217 1752
Size: 217 1535
BlpI GC'TnA_GC
Cuts at: 0 646 1002 1442 1480 1752
Size: 646 356 440 38 272
Fragments arranged by size:
646 440 356 272 38
Bme1580I G_kGCm'C
Cuts at: 0 131 534 1337 1365 1752
Size: 131 403 803 28 387
Fragments arranged by size:
803 403 387 131 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 1419 1752
Size: 1419 333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 307 502 889 1103 1752
Size: 82 225 195 387 214 649
Fragments arranged by size:
649 387 225 214 195 82
Bpu10I CC'TnA_GC
Cuts at: 0 605 1496 1592 1752
Size: 605 891 96 160
Fragments arranged by size:
891 605 160 96
BsaHI Gr'CG_yC
Cuts at: 0 228 1752
Size: 228 1524
BsaWI w'CCGG_w
Cuts at: 0 38 801 1752
Size: 38 763 951
Fragments arranged by size:
951 763 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 305 335 1450 1480 1752
Size: 305 30 1115 30 272
Fragments arranged by size:
1115 305 272 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 131 310 409 636 678 1030 1033
Size: 109 22 179 99 227 42 352 3
Cuts at: 1033 1752
Size: 719
Fragments arranged by size:
719 352 227 179 109 99 42 22
3
BseYI C'CCAG_C
Cuts at: 0 220 1159 1330 1752
Size: 220 939 171 422
Fragments arranged by size:
939 422 220 171
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1124 1752
Size: 1124 628
BsiHKAI G_wGCw'C
Cuts at: 0 178 1365 1543 1568 1752
Size: 178 1187 178 25 184
Fragments arranged by size:
1187 184 178 178 25
BsmI GAATG_Cn'
Cuts at: 0 692 1752
Size: 692 1060
BsmBI CGTCTCn'nnnn_
Cuts at: 0 711 1752
Size: 711 1041
Bsp1286I G_dGCh'C
Cuts at: 0 131 178 534 1337 1365 1463 1543 1568
Size: 131 47 356 803 28 98 80 25
Cuts at: 1568 1752
Size: 184
Fragments arranged by size:
803 356 184 131 98 80 47 28
25
BspEI T'CCGG_A
Cuts at: 0 38 801 1752
Size: 38 763 951
Fragments arranged by size:
951 763 38
BspHI T'CATG_A
Cuts at: 0 975 1752
Size: 975 777
BsrBI CCG'CTC
Cuts at: 0 645 1117 1743 1752
Size: 645 472 626 9
Fragments arranged by size:
645 626 472 9
BsrDI GCAATG_nn'
Cuts at: 0 1177 1752
Size: 1177 575
BsrFI r'CCGG_y
Cuts at: 0 1484 1752
Size: 1484 268
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1147 1752
Size: 1147 605
BstBI TT'CG_AA
Cuts at: 0 1394 1752
Size: 1394 358
BstEII G'GTnAC_C
Cuts at: 0 558 966 1752
Size: 558 408 786
Fragments arranged by size:
786 558 408
BstXI CCAn_nnnn'nTGG
Cuts at: 0 579 1167 1752
Size: 579 588 585
Fragments arranged by size:
588 585 579
BstYI r'GATC_y
Cuts at: 0 3 798 1206 1752
Size: 3 795 408 546
Fragments arranged by size:
795 546 408 3
Bsu36I CC'TnA_GG
Cuts at: 0 202 946 1616 1752
Size: 202 744 670 136
Fragments arranged by size:
744 670 202 136
BtgI C'CryG_G
Cuts at: 0 408 1436 1470 1752
Size: 408 1028 34 282
Fragments arranged by size:
1028 408 282 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 41 738 1752
Size: 41 697 1014
Fragments arranged by size:
1014 697 41
BtsI GCAGTG_nn'
Cuts at: 0 1136 1325 1365 1501 1752
Size: 1136 189 40 136 251
Fragments arranged by size:
1136 251 189 136 40
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 324 359 1752
Size: 324 35 1393
Fragments arranged by size:
1393 324 35
EaeI y'GGCC_r
Cuts at: 0 209 731 912 1554 1752
Size: 209 522 181 642 198
Fragments arranged by size:
642 522 209 198 181
EarI CTCTTCn'nnn_
Cuts at: 0 1019 1409 1752
Size: 1019 390 343
Fragments arranged by size:
1019 390 343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 307 502 889 1103 1752
Size: 82 225 195 387 214 649
Fragments arranged by size:
649 387 225 214 195 82
EcoNI CCTnn'n_nnAGG
Cuts at: 0 322 1303 1752
Size: 322 981 449
Fragments arranged by size:
981 449 322
EcoO109I rG'GnC_Cy
Cuts at: 0 127 307 469 513 1334 1371 1752
Size: 127 180 162 44 821 37 381
Fragments arranged by size:
821 381 180 162 127 44 37
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1420 1752
Size: 1420 332
HaeII r_GCGC'y
Cuts at: 0 231 1752
Size: 231 1521
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 1218 1250 1261 1293 1623 1655
Size: 37 32 1149 32 11 32 330 32
Cuts at: 1655 1752
Size: 97
Fragments arranged by size:
1149 330 97 37 32 32 32 32
11
HincII GTy'rAC
Cuts at: 0 1287 1752
Size: 1287 465
KasI G'GCGC_C
Cuts at: 0 227 1752
Size: 227 1525
MscI TGG'CCA
Cuts at: 0 211 914 1556 1752
Size: 211 703 642 196
Fragments arranged by size:
703 642 211 196
MspA1I CmG'CkG
Cuts at: 0 262 1752
Size: 262 1490
NaeI GCC'GGC
Cuts at: 0 1486 1752
Size: 1486 266
NarI GG'CG_CC
Cuts at: 0 228 1752
Size: 228 1524
NcoI C'CATG_G
Cuts at: 0 1436 1470 1752
Size: 1436 34 282
Fragments arranged by size:
1436 282 34
NgoMIV G'CCGG_C
Cuts at: 0 1484 1752
Size: 1484 268
NspI r_CATG'y
Cuts at: 0 771 1752
Size: 771 981
PasI CC'CwG_GG
Cuts at: 0 123 232 1752
Size: 123 109 1520
Fragments arranged by size:
1520 123 109
PflMI CCAn_nnn'nTGG
Cuts at: 0 867 1752
Size: 867 885
PfoI T'CCnGG_A
Cuts at: 0 323 1752
Size: 323 1429
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1450 1482 1752
Size: 1450 32 270
Fragments arranged by size:
1450 270 32
PpuMI rG'GwC_Cy
Cuts at: 0 469 513 1371 1752
Size: 469 44 858 381
Fragments arranged by size:
858 469 381 44
PshAI GACnn'nnGTC
Cuts at: 0 158 556 1752
Size: 158 398 1196
Fragments arranged by size:
1196 398 158
PspOMI G'GGCC_C
Cuts at: 0 127 1333 1752
Size: 127 1206 419
Fragments arranged by size:
1206 419 127
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1709 1741 1752
Size: 1709 32 11
Fragments arranged by size:
1709 32 11
PstI C_TGCA'G
Cuts at: 0 1511 1752
Size: 1511 241
PvuII CAG'CTG
Cuts at: 0 262 1752
Size: 262 1490
SanDI GG'GwC_CC
Cuts at: 0 469 1752
Size: 469 1283
SapI GCTCTTCn'nnn_
Cuts at: 0 1019 1409 1752
Size: 1019 390 343
Fragments arranged by size:
1019 390 343
ScaI AGT'ACT
Cuts at: 0 1045 1752
Size: 1045 707
SfcI C'TryA_G
Cuts at: 0 633 1184 1507 1726 1752
Size: 633 551 323 219 26
Fragments arranged by size:
633 551 323 219 26
SfoI GGC'GCC
Cuts at: 0 229 1752
Size: 229 1523
SmaI CCC'GGG
Cuts at: 0 474 1061 1369 1752
Size: 474 587 308 383
Fragments arranged by size:
587 474 383 308
StuI AGG'CCT
Cuts at: 0 919 1752
Size: 919 833
StyI C'CwwG_G
Cuts at: 0 1076 1436 1470 1752
Size: 1076 360 34 282
Fragments arranged by size:
1076 360 282 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 972 1158 1312 1752
Size: 972 186 154 440
Fragments arranged by size:
972 440 186 154
TatI w'GTAC_w
Cuts at: 0 26 1043 1752
Size: 26 1017 709
Fragments arranged by size:
1017 709 26
Tth111I GACn'n_nGTC
Cuts at: 0 460 1752
Size: 460 1292
XmaI C'CCGG_G
Cuts at: 0 472 1059 1367 1752
Size: 472 587 308 385
Fragments arranged by size:
587 472 385 308
XmnI GAAnn'nnTTC
Cuts at: 0 1390 1752
Size: 1390 362
Enzymes that do cut:
AccI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BcgI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrFI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI
MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PfoI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuII SanDI SapI ScaI SfcI SfoI SmaI StuI
StyI TaqII TatI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AscI AseI AsiSI AvrII BbsI
BciVI BclI BfrBI BglII BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsiEI BsiWI BspMI BsrGI BssHII
BssSI BstZ17I ClaI DraI DraIII DrdI EagI EciI
EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI MslI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PsiI PspXI
PvuI RsrII SacI SacII SalI SbfI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI XbaI XcmI XhoI ZraI