Restriction map for pF1KB9986
 (Linear) (Six-base) MAPSORT of: KIBB9986.seq  Check: 2120  from: 1  to: 2136



KIBB9986.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AatII G_ACGT'C

Cuts at:      0    1104    2136 
   Size:       1104    1032
 
AccI GT'mk_AC

Cuts at:      0    1104    1296    2136 
   Size:       1104     192     840

  Fragments arranged by size: 

                1104     840     192
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     967    1124    1549    1573    1728    2136 
   Size:        358     609     157     425      24     155     408

  Fragments arranged by size: 

                 609     425     408     358     157     155      24
 
AflII C'TTAA_G

Cuts at:      0    1020    2136 
   Size:       1020    1116
 
AflIII A'CryG_T

Cuts at:      0    1321    2136 
   Size:       1321     815
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1793    2136 
   Size:       1793     343
 
AlwNI CAG_nnn'CTG

Cuts at:      0     927    1377    1978    2136 
   Size:        927     450     601     158

  Fragments arranged by size: 

                 927     601     450     158
 
ApoI r'AATT_y

Cuts at:      0     223     258     296     727    1065    1642    1995    2136 
   Size:        223      35      38     431     338     577     353     141

  Fragments arranged by size: 

                 577     431     353     338     223     141      38      35
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     586    1246    1576    2104    2136 
   Size:        586     660     330     528      32

  Fragments arranged by size: 

                 660     586     528     330      32
 
BbvCI CC'TCA_GC

Cuts at:      0    1378    2136 
   Size:       1378     758
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1295    2136 
   Size:       1295     841
 
BfrBI ATG'CAT

Cuts at:      0    1360    2136 
   Size:       1360     776
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1560    2136 
   Size:       1560     576
 
BglII A'GATC_T

Cuts at:      0      62    2136 
   Size:         62    2074
 
BlpI GC'TnA_GC

Cuts at:      0     598    2136 
   Size:        598    1538
 
BmgBI CAC'GTC

Cuts at:      0     513    2136 
   Size:        513    1623
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1491    2136 
   Size:       1491     645
 
Bpu10I CC'TnA_GC

Cuts at:      0    1378    2136 
   Size:       1378     758
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1435    1816    2136 
   Size:       1435     381     320

  Fragments arranged by size: 

                1435     381     320
 
BsaAI yAC'GTr

Cuts at:      0    1125    2136 
   Size:       1125    1011
 
BsaHI Gr'CG_yC

Cuts at:      0    1101    2136 
   Size:       1101    1035
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1709    1739    2136 
   Size:       1709      30     397

  Fragments arranged by size: 

                1709     397      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     511     626    1366    1498    2136 
   Size:        511     115     740     132     638

  Fragments arranged by size: 

                 740     638     511     132     115
 
BseYI C'CCAG_C

Cuts at:      0    1013    2136 
   Size:       1013    1123
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1790    1891    2136 
   Size:       1790     101     245

  Fragments arranged by size: 

                1790     245     101
 
BsiHKAI G_wGCw'C

Cuts at:      0     851    2136 
   Size:        851    1285
 
BsmI GAATG_Cn'

Cuts at:      0    1865    2136 
   Size:       1865     271
 
Bsp1286I G_dGCh'C

Cuts at:      0     851    2136 
   Size:        851    1285
 
BspHI T'CATG_A

Cuts at:      0     339     948    2136 
   Size:        339     609    1188

  Fragments arranged by size: 

                1188     609     339
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1220    2136 
   Size:       1220     916
 
BsrDI GCAATG_nn'

Cuts at:      0     403    2136 
   Size:        403    1733
 
BsrGI T'GTAC_A

Cuts at:      0    1318    2136 
   Size:       1318     818
 
BssSI C'ACGA_G

Cuts at:      0    1936    2136 
   Size:       1936     200
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1999    2136 
   Size:       1999     137
 
BstBI TT'CG_AA

Cuts at:      0     131    2136 
   Size:        131    2005
 
BstYI r'GATC_y

Cuts at:      0      62    2136 
   Size:         62    2074
 
Bsu36I CC'TnA_GG

Cuts at:      0     110    2136 
   Size:        110    2026
 
DraI TTT'AAA

Cuts at:      0     900    1775    2136 
   Size:        900     875     361

  Fragments arranged by size: 

                 900     875     361
 
EarI CTCTTCn'nnn_

Cuts at:      0    1275    1280    2136 
   Size:       1275       5     856

  Fragments arranged by size: 

                1275     856       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     967    1124    1549    1573    1728    2136 
   Size:        358     609     157     425      24     155     408

  Fragments arranged by size: 

                 609     425     408     358     157     155      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     369    2136 
   Size:        369    1767
 
EcoRI G'AATT_C

Cuts at:      0     258    2136 
   Size:        258    1878
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      45     632    2136 
   Size:         45     587    1504

  Fragments arranged by size: 

                1504     587      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     472     504    1345    1377    1707    1739    2136 
   Size:        472      32     841      32     330      32     397

  Fragments arranged by size: 

                 841     472     397     330      32      32      32
 
HincII GTy'rAC

Cuts at:      0     715    1297    2136 
   Size:        715     582     839

  Fragments arranged by size: 

                 839     715     582
 
HindIII A'AGCT_T

Cuts at:      0     121     669    1136    2136 
   Size:        121     548     467    1000

  Fragments arranged by size: 

                1000     548     467     121
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     325    1122    2136 
   Size:        325     797    1014

  Fragments arranged by size: 

                1014     797     325
 
MslI CAynn'nnrTG

Cuts at:      0     722    1775    2136 
   Size:        722    1053     361

  Fragments arranged by size: 

                1053     722     361
 
MspA1I CmG'CkG

Cuts at:      0    1374    1975    2136 
   Size:       1374     601     161

  Fragments arranged by size: 

                1374     601     161
 
NdeI CA'TA_TG

Cuts at:      0     393    1226    1339    2136 
   Size:        393     833     113     797

  Fragments arranged by size: 

                 833     797     393     113
 
NsiI A_TGCA'T

Cuts at:      0    1362    2136 
   Size:       1362     774
 
NspI r_CATG'y

Cuts at:      0    1219    1325    2136 
   Size:       1219     106     811

  Fragments arranged by size: 

                1219     811     106
 
PciI A'CATG_T

Cuts at:      0    1321    2136 
   Size:       1321     815
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1734    2136 
   Size:       1734     402
 
PsiI TTA'TAA

Cuts at:      0     973    2080    2136 
   Size:        973    1107      56

  Fragments arranged by size: 

                1107     973      56
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     908     940    2136 
   Size:        908      32    1196

  Fragments arranged by size: 

                1196     908      32
 
PvuII CAG'CTG

Cuts at:      0    1374    1975    2136 
   Size:       1374     601     161

  Fragments arranged by size: 

                1374     601     161
 
SalI G'TCGA_C

Cuts at:      0    1295    2136 
   Size:       1295     841
 
ScaI AGT'ACT

Cuts at:      0     686    1652    2136 
   Size:        686     966     484

  Fragments arranged by size: 

                 966     686     484
 
SmlI C'TyrA_G

Cuts at:      0    1020    1414    1831    2136 
   Size:       1020     394     417     305

  Fragments arranged by size: 

                1020     417     394     305
 
StyI C'CwwG_G

Cuts at:      0    1458    2136 
   Size:       1458     678
 
SwaI ATTT'AAAT

Cuts at:      0    1775    2136 
   Size:       1775     361
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     532    2136 
   Size:        532    1604
 
TatI w'GTAC_w

Cuts at:      0     684    1313    1318    1650    1739    2136 
   Size:        684     629       5     332      89     397

  Fragments arranged by size: 

                 684     629     397     332      89       5
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1709    1741    2136 
   Size:       1709      32     395

  Fragments arranged by size: 

                1709     395      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     506    1234    1423    1700    2136 
   Size:        506     728     189     277     436

  Fragments arranged by size: 

                 728     506     436     277     189
 
ZraI GAC'GTC

Cuts at:      0    1102    2136 
   Size:       1102    1034
 

 Enzymes that do cut:

    AatII     AccI     AcuI    AflII   AflIII     AlfI    AlwNI     ApoI
     BbsI    BbvCI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI
    BssSI   BstAPI    BstBI    BstYI   Bsu36I     DraI     EarI  Eco57MI
 EcoO109I    EcoRI     FalI    Hin4I   HincII  HindIII     MmeI     MslI
   MspA1I     NdeI     NsiI     NspI     PciI    PflMI     PsiI     PsrI
    PvuII     SalI     ScaI     SmlI     StyI     SwaI    TaqII     TatI
     TstI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI     AgeI     AhdI     AleI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BcgI     BclI Bme1580I     BmtI
     BplI     BpmI     BsaI    BsaBI    BsaWI    BsiEI    BsiWI    BsmBI
    BspEI    BsrBI    BsrFI   BssHII   BstEII    BstXI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI
     PvuI    RsrII     SacI    SacII    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI  Tth111I     XbaI     XcmI     XhoI
     XmaI
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