Restriction map for pF1KB8485
 (Circular) (Six-base) MAPSORT of: pF1KB8485.seq  Check: 7592  from: 1  to: 5882



pF1KB8485  5882 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AccI GT'mk_AC

Cuts at:    836    2920     836 
   Size:       2084    3798
 
Acc65I G'GTAC_C

Cuts at:   2898    2898 
   Size:       5882
 
AclI AA'CG_TT

Cuts at:   5722    5722 
   Size:       5882
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    110     585     921    2437    3707    4139    4886    5154     110 
   Size:        475     336    1516    1270     432     747     268     838

  Fragments arranged by size: 

                1516    1270     838     747     475     432     336     268
            
 
AflIII A'CryG_T

Cuts at:    851    1158    1767    3234    4338    5315     851 
   Size:        307     609    1467    1104     977    1418

  Fragments arranged by size: 

                1467    1418    1104     977     609     307
 
AgeI A'CCGG_T

Cuts at:   4270    4270 
   Size:       5882
 
AhdI GACnn_n'nnGTC

Cuts at:    996    2319     996 
   Size:       1323    4559
 
AleI CACnn'nnGTG

Cuts at:   1268    1780    1268 
   Size:        512    5370
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1286    1441    2022    1286 
   Size:        155     581    5146

  Fragments arranged by size: 

                5146     581     155
 
AlwNI CAG_nnn'CTG

Cuts at:    291     588     711    1415    1896    2695    3187    4754     291 
   Size:        297     123     704     481     799     492    1567    1419

  Fragments arranged by size: 

                1567    1419     799     704     492     481     297     123
            
 
ApaI G_GGCC'C

Cuts at:    185     185 
   Size:       5882
 
ApaLI G'TGCA_C

Cuts at:   1281    4652    1281 
   Size:       3371    2511
 
ApoI r'AATT_y

Cuts at:   2886    5235    5491    2886 
   Size:       2349     256    3277

  Fragments arranged by size: 

                3277    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5882
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5882
 
AvaI C'yCGr_G

Cuts at:    178    1735    1813    2902     178 
   Size:       1557      78    1089    3158

  Fragments arranged by size: 

                3158    1557    1089      78
 
BamHI G'GATC_C

Cuts at:   2907    2907 
   Size:       5882
 
BanI G'GyrC_C

Cuts at:    781     844    1403    1547    2167    2254    2898    3312    3560 
   Size:         63     559     144     620      87     644     414     248

Cuts at:   3560    3595     781 
   Size:         35    3068

  Fragments arranged by size: 

                3068     644     620     559     414     248     144      87
                  63      35
 
BanII G_rGCy'C

Cuts at:    185     467     934    1234    1643    2195    2896    3926     185 
   Size:        282     467     300     409     552     701    1030    2141

  Fragments arranged by size: 

                2141    1030     701     552     467     409     300     282
            
 
BbeI G_GCGC'C

Cuts at:   1551    2258    3316    3564    1551 
   Size:        707    1058     248    3869

  Fragments arranged by size: 

                3869    1058     707     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    116     902     116 
   Size:        786    5096
 
BbvCI CC'TCA_GC

Cuts at:    292     292 
   Size:       5882
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    739     773    2851    2885     739 
   Size:         34    2078      34    3736

  Fragments arranged by size: 

                3736    2078      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3780    4541    5882    3780 
   Size:        761    1341    3780

  Fragments arranged by size: 

                3780    1341     761
 
BclI T'GATC_A

Cuts at:   1590    2357    3402    1590 
   Size:        767    1045    4070

  Fragments arranged by size: 

                4070    1045     767
 
BglI GCCn_nnn'nGGC

Cuts at:    406    1315    1534     406 
   Size:        909     219    4754

  Fragments arranged by size: 

                4754     909     219
 
BglII A'GATC_T

Cuts at:   1799    3397    5434    1799 
   Size:       1598    2037    2247

  Fragments arranged by size: 

                2247    2037    1598
 
BlpI GC'TnA_GC

Cuts at:   1132    2287    2994    1132 
   Size:       1155     707    4020

  Fragments arranged by size: 

                4020    1155     707
 
Bme1580I G_kGCm'C

Cuts at:    185     243     784     847    1285    1744    3507    3600    4656 
   Size:         58     541      63     438     459    1763      93    1056

Cuts at:   4656     185 
   Size:       1411

  Fragments arranged by size: 

                1763    1411    1056     541     459     438      93      63
                  58
 
BmgBI CAC'GTC

Cuts at:    753    1266     753 
   Size:        513    5369
 
BmrI ACTGGGnnnn_n'

Cuts at:   1196    1927    3094    3175    3510    5654    1196 
   Size:        731    1167      81     335    2144    1424

  Fragments arranged by size: 

                2144    1424    1167     731     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    948    1197    1674    1821    4249     948 
   Size:        249     477     147    2428    2581

  Fragments arranged by size: 

                2581    2428     477     249     147
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    409    1321    1348    1576    2724     409 
   Size:        912      27     228    1148    3567

  Fragments arranged by size: 

                3567    1148     912     228      27
 
Bpu10I CC'TnA_GC

Cuts at:    292    1228    1897     292 
   Size:        936     669    4277

  Fragments arranged by size: 

                4277     936     669
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3058    3937    4429    4727    3058 
   Size:        879     492     298    4213

  Fragments arranged by size: 

                4213     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1555    5586    1555 
   Size:       4031    1851
 
BsaAI yAC'GTr

Cuts at:    342    1161    1768    3235     342 
   Size:        819     607    1467    2989

  Fragments arranged by size: 

                2989    1467     819     607
 
BsaBI GATnn'nnATC

Cuts at:      6    3396    5433       6 
   Size:       3390    2037     455

  Fragments arranged by size: 

                3390    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1548    2255    2320    2570    3313    3561    4263    5759    1548 
   Size:        707      65     250     743     248     702    1496    1671

  Fragments arranged by size: 

                1671    1496     743     707     702     250     248      65
            
 
BsaWI w'CCGG_w

Cuts at:    323    1381    1577    2195    3071    3592    4270    4544    4691 
   Size:       1058     196     618     876     521     678     274     147

Cuts at:   4691    5102     323 
   Size:        411    1103

  Fragments arranged by size: 

                1103    1058     876     678     618     521     411     274
                 196     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    365     395    1346    1376    1406     365 
   Size:         30     951      30      30    4841

  Fragments arranged by size: 

                4841     951      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    181     880    1573    1647    2203     181 
   Size:        699     693      74     556    3860

  Fragments arranged by size: 

                3860     699     693     556      74
 
BseYI C'CCAG_C

Cuts at:    393     504     954    1295    2618    3307    4065    4642    5138 
   Size:        111     450     341    1323     689     758     577     496

Cuts at:   5138     393 
   Size:       1137

  Fragments arranged by size: 

                1323    1137     758     689     577     496     450     341
                 111
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    226     696    1098    1279    1804    2710     226 
   Size:        470     402     181     525     906    3398

  Fragments arranged by size: 

                3398     906     525     470     402     181
 
BsiEI CG_ry'CG

Cuts at:    104    1277    3424    3470    4678     104 
   Size:       1173    2147      46    1208    1308

  Fragments arranged by size: 

                2147    1308    1208    1173      46
 
BsiHKAI G_wGCw'C

Cuts at:    646    1017    1285    2195    2896    3674    3864    4656     646 
   Size:        371     268     910     701     778     190     792    1872

  Fragments arranged by size: 

                1872     910     792     778     701     371     268     190
            
 
BsmI GAATG_Cn'

Cuts at:   1462    1462 
   Size:       5882
 
Bsp1286I G_dGCh'C

Cuts at:    185     243     467     646     784     847     934    1017    1234 
   Size:         58     224     179     138      63      87      83     217

Cuts at:   1234    1285    1643    1744    2195    2896    3507    3600    3674 
   Size:         51     358     101     451     701     611      93      74

Cuts at:   3674    3864    3926    4656     185 
   Size:        190      62     730    1411

  Fragments arranged by size: 

                1411     730     701     611     451     358     224     217
                 190     179     138     101      93      87      83      74
                  63      62      58      51
 
BspEI T'CCGG_A

Cuts at:    323    2195    5102     323 
   Size:       1872    2907    1103

  Fragments arranged by size: 

                2907    1872    1103
 
BspHI T'CATG_A

Cuts at:   5058    5058 
   Size:       5882
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2511    2932    3146    3448    3829    2511 
   Size:        421     214     302     381    4564

  Fragments arranged by size: 

                4564     421     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1401    1901    4174    4228    5712    5877      50 
   Size:       1351     500    2273      54    1484     165      55

  Fragments arranged by size: 

                2273    1484    1351     500     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3794    3794 
   Size:       5882
 
BsrFI r'CCGG_y

Cuts at:   1544    2164    3880    4061    4270    1544 
   Size:        620    1716     181     209    3156

  Fragments arranged by size: 

                3156    1716     620     209     181
 
BssSI C'ACGA_G

Cuts at:   2870    4153    4511    2870 
   Size:       1283     358    4241

  Fragments arranged by size: 

                4241    1283     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3259    3259 
   Size:       5882
 
BstBI TT'CG_AA

Cuts at:   4243    4243 
   Size:       5882
 
BstEII G'GTnAC_C

Cuts at:   2835    2835 
   Size:       5882
 
BstXI CCAn_nnnn'nTGG

Cuts at:    270    1834    2626    2748     270 
   Size:       1564     792     122    3404

  Fragments arranged by size: 

                3404    1564     792     122
 
BstYI r'GATC_y

Cuts at:      1     254    1799    2441    2541    2657    2907    3374    3397 
   Size:        253    1545     642     100     116     250     467      23

Cuts at:   3397    3732    3978    4990    5076    5088    5434       1 
   Size:        335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1545    1012     642     467     449     346     335     253
                 250     246     116     100      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     278    1520    1628    2645     107 
   Size:        171    1242     108    1017    3344

  Fragments arranged by size: 

                3344    1242    1017     171     108
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    956    1475    1522    3832    4013    4176     956 
   Size:        519      47    2310     181     163    2662

  Fragments arranged by size: 

                2662    2310     519     181     163      47
 
BtsI GCAGTG_nn'

Cuts at:    412     540     684     890    2069    2696    3125     412 
   Size:        128     144     206    1179     627     429    3169

  Fragments arranged by size: 

                3169    1179     627     429     206     144     128
 
DraI TTT'AAA

Cuts at:   2881    2881 
   Size:       5882
 
DraIII CAC_nnn'GTG

Cuts at:    418    1860     418 
   Size:       1442    4440
 
DrdI GACnn_nn'nnGTC

Cuts at:   2112    3588    4446    2112 
   Size:       1476     858    3548

  Fragments arranged by size: 

                3548    1476     858
 
EaeI y'GGCC_r

Cuts at:    513    1274    1316    1376    1494    1631    2279    2753    3467 
   Size:        761      42      60     118     137     648     474     714

Cuts at:   3467    3641    4032    4059    5404     513 
   Size:        174     391      27    1345     991

  Fragments arranged by size: 

                1345     991     761     714     648     474     391     174
                 137     118      60      42      27
 
EagI C'GGCC_G

Cuts at:   1274    3467    1274 
   Size:       2193    3689
 
EarI CTCTTCn'nnn_

Cuts at:   2431    2675    3905    4115    2431 
   Size:        244    1230     210    4198

  Fragments arranged by size: 

                4198    1230     244     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2377    4090    4400    4546    5692    2377 
   Size:       1713     310     146    1146    2567

  Fragments arranged by size: 

                2567    1713    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   2193    2894    2193 
   Size:        701    5181
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    110     409     585     921    1321    1348    1576    2437    2724 
   Size:        299     176     336     400      27     228     861     287

Cuts at:   2724    3707    4139    4886    5154     110 
   Size:        983     432     747     268     838

  Fragments arranged by size: 

                 983     861     838     747     432     400     336     299
                 287     268     228     176      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1555    1960    2518    1555 
   Size:        405     558    4919

  Fragments arranged by size: 

                4919     558     405
 
EcoO109I rG'GnC_Cy

Cuts at:    182     407     737    1098    1176    1893    2130    2673    3021 
   Size:        225     330     361      78     717     237     543     348

Cuts at:   3021     182 
   Size:       3043

  Fragments arranged by size: 

                3043     717     543     361     348     330     237     225
                  78
 
EcoRI G'AATT_C

Cuts at:   2886    2886 
   Size:       5882
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3901    3901 
   Size:       5882
 
FspI TGC'GCA

Cuts at:    566    2861    3663     566 
   Size:       2295     802    2785

  Fragments arranged by size: 

                2785    2295     802
 
HaeII r_GCGC'y

Cuts at:    292     955    1551    2258    2451    3316    3564    4586    5565 
   Size:        663     596     707     193     865     248    1022     979

Cuts at:   5565     292 
   Size:        609

  Fragments arranged by size: 

                1022     979     865     707     663     609     596     248
                 193
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    982    1014    2257    2289    2305    2337    2797    2829    3724 
   Size:         32    1243      32      16      32     460      32     895

Cuts at:   3724    3754    3756    3786     982 
   Size:         30       2      30    3078

  Fragments arranged by size: 

                3078    1243     895     460      32      32      32      32
                  30      30      16       2
 
HincII GTy'rAC

Cuts at:   2867    2921    2867 
   Size:         54    5828
 
KasI G'GCGC_C

Cuts at:   1547    2254    3312    3560    1547 
   Size:        707    1058     248    3869

  Fragments arranged by size: 

                3869    1058     707     248
 
KpnI G_GTAC'C

Cuts at:   2902    2902 
   Size:       5882
 
MluI A'CGCG_T

Cuts at:    851    5315     851 
   Size:       4464    1418
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2139    4553    4737    2139 
   Size:       2414     184    3284

  Fragments arranged by size: 

                3284    2414     184
 
MscI TGG'CCA

Cuts at:    515    1378    1633    2281    3643     515 
   Size:        863     255     648    1362    2754

  Fragments arranged by size: 

                2754    1362     863     648     255
 
MslI CAynn'nnrTG

Cuts at:    751     787    1268    1780    2242    3871    3998     751 
   Size:         36     481     512     462    1629     127    2635

  Fragments arranged by size: 

                2635    1629     512     481     462     127      36
 
MspA1I CmG'CkG

Cuts at:    708    1321    2363    2842    2993    3307    3667    4680    4925 
   Size:        613    1042     479     151     314     360    1013     245

Cuts at:   4925     708 
   Size:       1665

  Fragments arranged by size: 

                1665    1042    1013     613     479     360     314     245
                 151
 
NaeI GCC'GGC

Cuts at:   4063    4063 
   Size:       5882
 
NarI GG'CG_CC

Cuts at:   1548    2255    3313    3561    1548 
   Size:        707    1058     248    3869

  Fragments arranged by size: 

                3869    1058     707     248
 
NcoI C'CATG_G

Cuts at:    107     278    1628     107 
   Size:        171    1350    4361

  Fragments arranged by size: 

                4361    1350     171
 
NgoMIV G'CCGG_C

Cuts at:   4061    4061 
   Size:       5882
 
NspI r_CATG'y

Cuts at:    880    1671    2235    2370    2935    3435    4342     880 
   Size:        791     564     135     565     500     907    2420

  Fragments arranged by size: 

                2420     907     791     565     564     500     135
 
PasI CC'CwG_GG

Cuts at:   1234    1827    1234 
   Size:        593    5289
 
PciI A'CATG_T

Cuts at:   4338    4338 
   Size:       5882
 
PflMI CCAn_nnn'nTGG

Cuts at:    699     987    1860     699 
   Size:        288     873    4721

  Fragments arranged by size: 

                4721     873     288
 
PfoI T'CCnGG_A

Cuts at:    557     655    1151    2660     557 
   Size:         98     496    1509    3779

  Fragments arranged by size: 

                3779    1509     496      98
 
PmeI GTTT'AAAC

Cuts at:   2881    2881 
   Size:       5882
 
PmlI CAC'GTG

Cuts at:   1161    1161 
   Size:       5882
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5021    5053    5021 
   Size:         32    5850
 
PpuMI rG'GwC_Cy

Cuts at:   1098    1176    2130    1098 
   Size:         78     954    4850

  Fragments arranged by size: 

                4850     954      78
 
PshAI GACnn'nnGTC

Cuts at:   2872    2872 
   Size:       5882
 
PspOMI G'GGCC_C

Cuts at:    181     181 
   Size:       5882
 
PstI C_TGCA'G

Cuts at:    221     593    1217    1420    1430    2801    2929    3614     221 
   Size:        372     624     203      10    1371     128     685    2489

  Fragments arranged by size: 

                2489    1371     685     624     372     203     128      10
            
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5882
 
PvuII CAG'CTG

Cuts at:    708    3307    3667     708 
   Size:       2599     360    2923

  Fragments arranged by size: 

                2923    2599     360
 
RsrII CG'GwC_CG

Cuts at:    321    4077     321 
   Size:       3756    2126
 
SacI G_AGCT'C

Cuts at:   2195    2896    2195 
   Size:        701    5181
 
SalI G'TCGA_C

Cuts at:   2919    2919 
   Size:       5882
 
SapI GCTCTTCn'nnn_

Cuts at:   2675    3905    4115    2675 
   Size:       1230     210    4442

  Fragments arranged by size: 

                4442    1230     210
 
SbfI CC_TGCA'GG

Cuts at:   1217    1430    2929    1217 
   Size:        213    1499    4170

  Fragments arranged by size: 

                4170    1499     213
 
ScaI AGT'ACT

Cuts at:   5399    5399 
   Size:       5882
 
SfcI C'TryA_G

Cuts at:     33     217     247     589    1213    1416    1426    2797    2925 
   Size:        184      30     342     624     203      10    1371     128

Cuts at:   2925    3610    4603    4794      33 
   Size:        685     993     191    1121

  Fragments arranged by size: 

                1371    1121     993     685     624     342     203     191
                 184     128      30      10
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    406     406 
   Size:       5882
 
SfoI GGC'GCC

Cuts at:   1549    2256    3314    3562    1549 
   Size:        707    1058     248    3869

  Fragments arranged by size: 

                3869    1058     707     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5882
 
SgrAI Cr'CCGG_yG

Cuts at:   1544    1544 
   Size:       5882
 
SmaI CCC'GGG

Cuts at:    180    2904     180 
   Size:       2724    3158
 
SmlI C'TyrA_G

Cuts at:   3037    3952    4444    4706    3037 
   Size:        915     492     262    4213

  Fragments arranged by size: 

                4213     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5882
 
SphI G_CATG'C

Cuts at:   1671    2935    3435    1671 
   Size:       1264     500    4118

  Fragments arranged by size: 

                4118    1264     500
 
SrfI GCCC'GGGC

Cuts at:    180     180 
   Size:       5882
 
StuI AGG'CCT

Cuts at:   1043    1654    2150    1043 
   Size:        611     496    4775

  Fragments arranged by size: 

                4775     611     496
 
StyI C'CwwG_G

Cuts at:    107     278    1137    1628    1634    1985    2415    3016    3370 
   Size:        171     859     491       6     351     430     601     354

Cuts at:   3370     107 
   Size:       2619

  Fragments arranged by size: 

                2619     859     601     491     430     354     351     171
                   6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    417     496    1042    3465    3598    4057    4266     417 
   Size:         79     546    2423     133     459     209    2033

  Fragments arranged by size: 

                2423    2033     546     459     209     133      79
 
TatI w'GTAC_w

Cuts at:   5397    5397 
   Size:       5882
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    365     397    1376    1408    5399    5431     365 
   Size:         32     979      32    3991      32     816

  Fragments arranged by size: 

                3991     979     816      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3679    3679 
   Size:       5882
 
XbaI T'CTAG_A

Cuts at:   2913    2913 
   Size:       5882
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3377    3377 
   Size:       5882
 
XmaI C'CCGG_G

Cuts at:    178    2902     178 
   Size:       2724    3158
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI
     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AloI     AscI    AvrII     BaeI
    BfrBI     BmtI    BsiWI    BsmBI    BsrGI   BssHII  BstZ17I   Bsu36I
     ClaI    CspCI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PsiI    PspXI
     PsrI    SacII    SanDI    SexAI    SnaBI     SspI     SwaI     XhoI
     XmnI     ZraI
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