Restriction map for pF1KB8485
 (Linear) (Six-base) MAPSORT of: KIBB8485.seq  Check: 2377  from: 1  to: 2769



KIBB8485.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AccI GT'mk_AC

Cuts at:      0     728    2769 
   Size:        728    2041
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2     477     813    2329    2769 
   Size:          2     475     336    1516     440

  Fragments arranged by size: 

                1516     475     440     336       2
 
AflIII A'CryG_T

Cuts at:      0     743    1050    1659    2769 
   Size:        743     307     609    1110

  Fragments arranged by size: 

                1110     743     609     307
 
AhdI GACnn_n'nnGTC

Cuts at:      0     888    2211    2769 
   Size:        888    1323     558

  Fragments arranged by size: 

                1323     888     558
 
AleI CACnn'nnGTG

Cuts at:      0    1160    1672    2769 
   Size:       1160     512    1097

  Fragments arranged by size: 

                1160    1097     512
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1178    1333    1914    2769 
   Size:       1178     155     581     855

  Fragments arranged by size: 

                1178     855     581     155
 
AlwNI CAG_nnn'CTG

Cuts at:      0     183     480     603    1307    1788    2587    2769 
   Size:        183     297     123     704     481     799     182

  Fragments arranged by size: 

                 799     704     481     297     183     182     123
 
ApaI G_GGCC'C

Cuts at:      0      77    2769 
   Size:         77    2692
 
ApaLI G'TGCA_C

Cuts at:      0    1173    2769 
   Size:       1173    1596
 
AvaI C'yCGr_G

Cuts at:      0      70    1627    1705    2769 
   Size:         70    1557      78    1064

  Fragments arranged by size: 

                1557    1064      78      70
 
BanI G'GyrC_C

Cuts at:      0     673     736    1295    1439    2059    2146    2769 
   Size:        673      63     559     144     620      87     623

  Fragments arranged by size: 

                 673     623     620     559     144      87      63
 
BanII G_rGCy'C

Cuts at:      0      77     359     826    1126    1535    2087    2769 
   Size:         77     282     467     300     409     552     682

  Fragments arranged by size: 

                 682     552     467     409     300     282      77
 
BbeI G_GCGC'C

Cuts at:      0    1443    2150    2769 
   Size:       1443     707     619

  Fragments arranged by size: 

                1443     707     619
 
BbsI GAAGACnn'nnnn_

Cuts at:      0       8     794    2769 
   Size:          8     786    1975

  Fragments arranged by size: 

                1975     786       8
 
BbvCI CC'TCA_GC

Cuts at:      0     184    2769 
   Size:        184    2585
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     631     665    2743    2769 
   Size:        631      34    2078      26

  Fragments arranged by size: 

                2078     631      34      26
 
BclI T'GATC_A

Cuts at:      0    1482    2249    2769 
   Size:       1482     767     520

  Fragments arranged by size: 

                1482     767     520
 
BglI GCCn_nnn'nGGC

Cuts at:      0     298    1207    1426    2769 
   Size:        298     909     219    1343

  Fragments arranged by size: 

                1343     909     298     219
 
BglII A'GATC_T

Cuts at:      0    1691    2769 
   Size:       1691    1078
 
BlpI GC'TnA_GC

Cuts at:      0    1024    2179    2769 
   Size:       1024    1155     590

  Fragments arranged by size: 

                1155    1024     590
 
Bme1580I G_kGCm'C

Cuts at:      0      77     135     676     739    1177    1636    2769 
   Size:         77      58     541      63     438     459    1133

  Fragments arranged by size: 

                1133     541     459     438      77      63      58
 
BmgBI CAC'GTC

Cuts at:      0     645    1158    2769 
   Size:        645     513    1611

  Fragments arranged by size: 

                1611     645     513
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1088    1819    2769 
   Size:       1088     731     950

  Fragments arranged by size: 

                1088     950     731
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     840    1089    1566    1713    2769 
   Size:        840     249     477     147    1056

  Fragments arranged by size: 

                1056     840     477     249     147
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301    1213    1240    1468    2616    2769 
   Size:        301     912      27     228    1148     153

  Fragments arranged by size: 

                1148     912     301     228     153      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     184    1120    1789    2769 
   Size:        184     936     669     980

  Fragments arranged by size: 

                 980     936     669     184
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1447    2769 
   Size:       1447    1322
 
BsaAI yAC'GTr

Cuts at:      0     234    1053    1660    2769 
   Size:        234     819     607    1109

  Fragments arranged by size: 

                1109     819     607     234
 
BsaHI Gr'CG_yC

Cuts at:      0    1440    2147    2212    2462    2769 
   Size:       1440     707      65     250     307

  Fragments arranged by size: 

                1440     707     307     250      65
 
BsaWI w'CCGG_w

Cuts at:      0     215    1273    1469    2087    2769 
   Size:        215    1058     196     618     682

  Fragments arranged by size: 

                1058     682     618     215     196
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     257     287    1238    1268    1298    2769 
   Size:        257      30     951      30      30    1471

  Fragments arranged by size: 

                1471     951     257      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73     772    1465    1539    2095    2769 
   Size:         73     699     693      74     556     674

  Fragments arranged by size: 

                 699     693     674     556      74      73
 
BseYI C'CCAG_C

Cuts at:      0     285     396     846    1187    2510    2769 
   Size:        285     111     450     341    1323     259

  Fragments arranged by size: 

                1323     450     341     285     259     111
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     588     990    1171    1696    2602    2769 
   Size:        118     470     402     181     525     906     167

  Fragments arranged by size: 

                 906     525     470     402     181     167     118
 
BsiEI CG_ry'CG

Cuts at:      0    1169    2769 
   Size:       1169    1600
 
BsiHKAI G_wGCw'C

Cuts at:      0     538     909    1177    2087    2769 
   Size:        538     371     268     910     682

  Fragments arranged by size: 

                 910     682     538     371     268
 
BsmI GAATG_Cn'

Cuts at:      0    1354    2769 
   Size:       1354    1415
 
Bsp1286I G_dGCh'C

Cuts at:      0      77     135     359     538     676     739     826     909 
   Size:         77      58     224     179     138      63      87      83

Cuts at:    909    1126    1177    1535    1636    2087    2769 
   Size:        217      51     358     101     451     682

  Fragments arranged by size: 

                 682     451     358     224     217     179     138     101
                  87      83      77      63      58      51
 
BspEI T'CCGG_A

Cuts at:      0     215    2087    2769 
   Size:        215    1872     682

  Fragments arranged by size: 

                1872     682     215
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2403    2769 
   Size:       2403     366
 
BsrBI CCG'CTC

Cuts at:      0    1293    1793    2769 
   Size:       1293     500     976

  Fragments arranged by size: 

                1293     976     500
 
BsrFI r'CCGG_y

Cuts at:      0    1436    2056    2769 
   Size:       1436     620     713

  Fragments arranged by size: 

                1436     713     620
 
BssSI C'ACGA_G

Cuts at:      0    2762    2769 
   Size:       2762       7
 
BstEII G'GTnAC_C

Cuts at:      0    2727    2769 
   Size:       2727      42
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     162    1726    2518    2640    2769 
   Size:        162    1564     792     122     129

  Fragments arranged by size: 

                1564     792     162     129     122
 
BstYI r'GATC_y

Cuts at:      0     146    1691    2333    2433    2549    2769 
   Size:        146    1545     642     100     116     220

  Fragments arranged by size: 

                1545     642     220     146     116     100
 
BtgI C'CryG_G

Cuts at:      0     170    1412    1520    2537    2769 
   Size:        170    1242     108    1017     232

  Fragments arranged by size: 

                1242    1017     232     170     108
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     848    1367    1414    2769 
   Size:        848     519      47    1355

  Fragments arranged by size: 

                1355     848     519      47
 
BtsI GCAGTG_nn'

Cuts at:      0     304     432     576     782    1961    2588    2769 
   Size:        304     128     144     206    1179     627     181

  Fragments arranged by size: 

                1179     627     304     206     181     144     128
 
DraIII CAC_nnn'GTG

Cuts at:      0     310    1752    2769 
   Size:        310    1442    1017

  Fragments arranged by size: 

                1442    1017     310
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2004    2769 
   Size:       2004     765
 
EaeI y'GGCC_r

Cuts at:      0     405    1166    1208    1268    1386    1523    2171    2645 
   Size:        405     761      42      60     118     137     648     474

Cuts at:   2645    2769 
   Size:        124

  Fragments arranged by size: 

                 761     648     474     405     137     124     118      60
                  42
 
EagI C'GGCC_G

Cuts at:      0    1166    2769 
   Size:       1166    1603
 
EarI CTCTTCn'nnn_

Cuts at:      0    2323    2567    2769 
   Size:       2323     244     202

  Fragments arranged by size: 

                2323     244     202
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2269    2769 
   Size:       2269     500
 
EcoICRI GAG'CTC

Cuts at:      0    2085    2769 
   Size:       2085     684
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2     301     477     813    1213    1240    1468    2329 
   Size:          2     299     176     336     400      27     228     861

Cuts at:   2329    2616    2769 
   Size:        287     153

  Fragments arranged by size: 

                 861     400     336     299     287     228     176     153
                  27       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1447    1852    2410    2769 
   Size:       1447     405     558     359

  Fragments arranged by size: 

                1447     558     405     359
 
EcoO109I rG'GnC_Cy

Cuts at:      0      74     299     629     990    1068    1785    2022    2565 
   Size:         74     225     330     361      78     717     237     543

Cuts at:   2565    2769 
   Size:        204

  Fragments arranged by size: 

                 717     543     361     330     237     225     204      78
                  74
 
FspI TGC'GCA

Cuts at:      0     458    2753    2769 
   Size:        458    2295      16

  Fragments arranged by size: 

                2295     458      16
 
HaeII r_GCGC'y

Cuts at:      0     184     847    1443    2150    2343    2769 
   Size:        184     663     596     707     193     426

  Fragments arranged by size: 

                 707     663     596     426     193     184
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     874     906    2149    2181    2197    2229    2689    2721 
   Size:        874      32    1243      32      16      32     460      32

Cuts at:   2721    2769 
   Size:         48

  Fragments arranged by size: 

                1243     874     460      48      32      32      32      32
                  16
 
HincII GTy'rAC

Cuts at:      0    2759    2769 
   Size:       2759      10
 
KasI G'GCGC_C

Cuts at:      0    1439    2146    2769 
   Size:       1439     707     623

  Fragments arranged by size: 

                1439     707     623
 
MluI A'CGCG_T

Cuts at:      0     743    2769 
   Size:        743    2026
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2031    2769 
   Size:       2031     738
 
MscI TGG'CCA

Cuts at:      0     407    1270    1525    2173    2769 
   Size:        407     863     255     648     596

  Fragments arranged by size: 

                 863     648     596     407     255
 
MslI CAynn'nnrTG

Cuts at:      0     643     679    1160    1672    2134    2769 
   Size:        643      36     481     512     462     635

  Fragments arranged by size: 

                 643     635     512     481     462      36
 
MspA1I CmG'CkG

Cuts at:      0     600    1213    2255    2734    2769 
   Size:        600     613    1042     479      35

  Fragments arranged by size: 

                1042     613     600     479      35
 
NarI GG'CG_CC

Cuts at:      0    1440    2147    2769 
   Size:       1440     707     622

  Fragments arranged by size: 

                1440     707     622
 
NcoI C'CATG_G

Cuts at:      0     170    1520    2769 
   Size:        170    1350    1249

  Fragments arranged by size: 

                1350    1249     170
 
NspI r_CATG'y

Cuts at:      0     772    1563    2127    2262    2769 
   Size:        772     791     564     135     507

  Fragments arranged by size: 

                 791     772     564     507     135
 
PasI CC'CwG_GG

Cuts at:      0    1126    1719    2769 
   Size:       1126     593    1050

  Fragments arranged by size: 

                1126    1050     593
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     591     879    1752    2769 
   Size:        591     288     873    1017

  Fragments arranged by size: 

                1017     873     591     288
 
PfoI T'CCnGG_A

Cuts at:      0     449     547    1043    2552    2769 
   Size:        449      98     496    1509     217

  Fragments arranged by size: 

                1509     496     449     217      98
 
PmlI CAC'GTG

Cuts at:      0    1053    2769 
   Size:       1053    1716
 
PpuMI rG'GwC_Cy

Cuts at:      0     990    1068    2022    2769 
   Size:        990      78     954     747

  Fragments arranged by size: 

                 990     954     747      78
 
PshAI GACnn'nnGTC

Cuts at:      0    2764    2769 
   Size:       2764       5
 
PspOMI G'GGCC_C

Cuts at:      0      73    2769 
   Size:         73    2696
 
PstI C_TGCA'G

Cuts at:      0     113     485    1109    1312    1322    2693    2769 
   Size:        113     372     624     203      10    1371      76

  Fragments arranged by size: 

                1371     624     372     203     113      76      10
 
PvuII CAG'CTG

Cuts at:      0     600    2769 
   Size:        600    2169
 
RsrII CG'GwC_CG

Cuts at:      0     213    2769 
   Size:        213    2556
 
SacI G_AGCT'C

Cuts at:      0    2087    2769 
   Size:       2087     682
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2567    2769 
   Size:       2567     202
 
SbfI CC_TGCA'GG

Cuts at:      0    1109    1322    2769 
   Size:       1109     213    1447

  Fragments arranged by size: 

                1447    1109     213
 
SfcI C'TryA_G

Cuts at:      0     109     139     481    1105    1308    1318    2689    2769 
   Size:        109      30     342     624     203      10    1371      80

  Fragments arranged by size: 

                1371     624     342     203     109      80      30      10
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     298    2769 
   Size:        298    2471
 
SfoI GGC'GCC

Cuts at:      0    1441    2148    2769 
   Size:       1441     707     621

  Fragments arranged by size: 

                1441     707     621
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1436    2769 
   Size:       1436    1333
 
SmaI CCC'GGG

Cuts at:      0      72    2769 
   Size:         72    2697
 
SphI G_CATG'C

Cuts at:      0    1563    2769 
   Size:       1563    1206
 
SrfI GCCC'GGGC

Cuts at:      0      72    2769 
   Size:         72    2697
 
StuI AGG'CCT

Cuts at:      0     935    1546    2042    2769 
   Size:        935     611     496     727

  Fragments arranged by size: 

                 935     727     611     496
 
StyI C'CwwG_G

Cuts at:      0     170    1029    1520    1526    1877    2307    2769 
   Size:        170     859     491       6     351     430     462

  Fragments arranged by size: 

                 859     491     462     430     351     170       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     309     388     934    2769 
   Size:        309      79     546    1835

  Fragments arranged by size: 

                1835     546     309      79
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     257     289    1268    1300    2769 
   Size:        257      32     979      32    1469

  Fragments arranged by size: 

                1469     979     257      32      32
 
XmaI C'CCGG_G

Cuts at:      0      70    2769 
   Size:         70    2699
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI     AleI     AlfI    AlwNI     ApaI
    ApaLI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BssSI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I   HincII     KasI
     MluI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI     PstI
    PvuII    RsrII     SacI     SapI     SbfI     SfcI     SfiI     SfoI
    SgrAI     SmaI     SphI     SrfI     StuI     StyI    TaqII     TstI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI
    BfrBI     BmtI    BpuEI    BsaBI    BsiWI    BsmBI    BspHI    BsrDI
    BsrGI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI    EcoRI    EcoRV     FalI     FseI    FspAI  HindIII     HpaI
     KpnI     MfeI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PpiI     PsiI    PspXI     PsrI
     PvuI    SacII     SalI    SanDI     ScaI    SexAI     SgfI     SmlI
    SnaBI     SpeI     SspI     SwaI     TatI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI
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