Restriction map for pF1KB5929
 (Linear) (Six-base) MAPSORT of: KIBB5929.seq  Check: 1683  from: 1  to: 1521



KIBB5929.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

Acc65I G'GTAC_C

Cuts at:      0      37    1521 
   Size:         37    1484
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294    1030    1085    1367    1521 
   Size:        294     736      55     282     154

  Fragments arranged by size: 

                 736     294     282     154      55
 
AlwNI CAG_nnn'CTG

Cuts at:      0     109    1521 
   Size:        109    1412
 
ApoI r'AATT_y

Cuts at:      0     487    1181    1521 
   Size:        487     694     340

  Fragments arranged by size: 

                 694     487     340
 
AvaI C'yCGr_G

Cuts at:      0      24    1521 
   Size:         24    1497
 
BanI G'GyrC_C

Cuts at:      0      28      37    1521 
   Size:         28       9    1484

  Fragments arranged by size: 

                1484      28       9
 
BanII G_rGCy'C

Cuts at:      0      21    1521 
   Size:         21    1500
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1011    1521 
   Size:       1011     510
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1236    1521 
   Size:       1236     285
 
BclI T'GATC_A

Cuts at:      0     705    1521 
   Size:        705     816
 
BglI GCCn_nnn'nGGC

Cuts at:      0      55    1210    1521 
   Size:         55    1155     311

  Fragments arranged by size: 

                1155     311      55
 
BglII A'GATC_T

Cuts at:      0    1342    1371    1521 
   Size:       1342      29     150

  Fragments arranged by size: 

                1342     150      29
 
Bme1580I G_kGCm'C

Cuts at:      0      31     688    1521 
   Size:         31     657     833

  Fragments arranged by size: 

                 833     657      31
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     262     330     539    1521 
   Size:        262      68     209     982

  Fragments arranged by size: 

                 982     262     209      68
 
Bpu10I CC'TnA_GC

Cuts at:      0    1420    1521 
   Size:       1420     101
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     310    1521 
   Size:        310    1211
 
BsaBI GATnn'nnATC

Cuts at:      0    1370    1435    1521 
   Size:       1370      65      86

  Fragments arranged by size: 

                1370      86      65
 
BsaWI w'CCGG_w

Cuts at:      0      34    1521 
   Size:         34    1487
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     312     342    1521 
   Size:        312      30    1179

  Fragments arranged by size: 

                1179     312      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     854    1521 
   Size:        854     667
 
BsiHKAI G_wGCw'C

Cuts at:      0     369    1521 
   Size:        369    1152
 
BsmI GAATG_Cn'

Cuts at:      0     112    1521 
   Size:        112    1409
 
Bsp1286I G_dGCh'C

Cuts at:      0      21      31     369     688    1521 
   Size:         21      10     338     319     833

  Fragments arranged by size: 

                 833     338     319      21      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      94    1521 
   Size:         94    1427
 
BsrDI GCAATG_nn'

Cuts at:      0     634     694    1521 
   Size:        634      60     827

  Fragments arranged by size: 

                 827     634      60
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     109    1521 
   Size:        109    1412
 
BstBI TT'CG_AA

Cuts at:      0     606    1521 
   Size:        606     915
 
BstYI r'GATC_y

Cuts at:      0    1140    1342    1371    1521 
   Size:       1140     202      29     150

  Fragments arranged by size: 

                1140     202     150      29
 
BtgI C'CryG_G

Cuts at:      0      53      62    1521 
   Size:         53       9    1459

  Fragments arranged by size: 

                1459      53       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     313     756    1186    1214    1521 
   Size:        313     443     430      28     307

  Fragments arranged by size: 

                 443     430     313     307      28
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     467    1521 
   Size:        467    1054
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294    1030    1085    1367    1521 
   Size:        294     736      55     282     154

  Fragments arranged by size: 

                 736     294     282     154      55
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1164    1521 
   Size:       1164     357
 
EcoRI G'AATT_C

Cuts at:      0     487    1521 
   Size:        487    1034
 
EcoRV GAT'ATC

Cuts at:      0    1056    1521 
   Size:       1056     465
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1040    1045    1072    1077    1308    1340    1496    1521 
   Size:       1040       5      27       5     231      32     156      25

  Fragments arranged by size: 

                1040     231     156      32      27      25       5       5
            
 
KpnI G_GTAC'C

Cuts at:      0      41    1521 
   Size:         41    1480
 
MfeI C'AATT_G

Cuts at:      0     817    1521 
   Size:        817     704
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     403    1521 
   Size:        365      38    1118

  Fragments arranged by size: 

                1118     365      38
 
MspA1I CmG'CkG

Cuts at:      0      43      55      64     348    1239    1521 
   Size:         43      12       9     284     891     282

  Fragments arranged by size: 

                 891     284     282      43      12       9
 
NspI r_CATG'y

Cuts at:      0     543    1521 
   Size:        543     978
 
PpuMI rG'GwC_Cy

Cuts at:      0    1164    1521 
   Size:       1164     357
 
PstI C_TGCA'G

Cuts at:      0      48     105     434    1521 
   Size:         48      57     329    1087

  Fragments arranged by size: 

                1087     329      57      48
 
PvuII CAG'CTG

Cuts at:      0     348    1239    1521 
   Size:        348     891     282

  Fragments arranged by size: 

                 891     348     282
 
SacII CC_GC'GG

Cuts at:      0      56      65    1521 
   Size:         56       9    1456

  Fragments arranged by size: 

                1456      56       9
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1214    1521 
   Size:       1214     307
 
ScaI AGT'ACT

Cuts at:      0     222    1521 
   Size:        222    1299
 
SfcI C'TryA_G

Cuts at:      0      44     101     430    1103    1521 
   Size:         44      57     329     673     418

  Fragments arranged by size: 

                 673     418     329      57      44
 
SpeI A'CTAG_T

Cuts at:      0     217    1521 
   Size:        217    1304
 
SphI G_CATG'C

Cuts at:      0     543    1521 
   Size:        543     978
 
SspI AAT'ATT

Cuts at:      0     474    1521 
   Size:        474    1047
 
StuI AGG'CCT

Cuts at:      0     436    1418    1521 
   Size:        436     982     103

  Fragments arranged by size: 

                 982     436     103
 
StyI C'CwwG_G

Cuts at:      0     935    1521 
   Size:        935     586
 
TatI w'GTAC_w

Cuts at:      0     220    1521 
   Size:        220    1301
 
XbaI T'CTAG_A

Cuts at:      0    1452    1521 
   Size:       1452      69
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1504    1521 
   Size:       1504      17
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI     ApoI     AvaI     BanI    BanII     BbsI
    BciVI     BclI     BglI    BglII Bme1580I     BmrI   Bpu10I     BsaI
    BsaBI    BsaWI    BsaXI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrDI   BstAPI    BstBI    BstYI     BtgI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI    EcoRV    Hin4I     KpnI     MfeI     MmeI   MspA1I
     NspI    PpuMI     PstI    PvuII    SacII     SapI     ScaI     SfcI
     SpeI     SphI     SspI     StuI     StyI     TatI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BfrBI
     BlpI    BmgBI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaHI
    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI  EcoICRI    EcoNI     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SrfI     SwaI    TaqII     TstI  Tth111I
     XhoI     XmaI     XmnI     ZraI
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