Restriction map for pF1KB5799
 (Linear) (Six-base) MAPSORT of: KIBB5799.seq  Check: 5297  from: 1  to: 2502



KIBB5799.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AatII G_ACGT'C

Cuts at:      0     869    1592    2502 
   Size:        869     723     910

  Fragments arranged by size: 

                 910     869     723
 
AccI GT'mk_AC

Cuts at:      0    1883    2502 
   Size:       1883     619
 
AclI AA'CG_TT

Cuts at:      0    2456    2502 
   Size:       2456      46
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     763    2502 
   Size:        580     183    1739

  Fragments arranged by size: 

                1739     580     183
 
AflIII A'CryG_T

Cuts at:      0     823    2502 
   Size:        823    1679
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1867    2502 
   Size:       1867     635
 
AlwNI CAG_nnn'CTG

Cuts at:      0     897     915     995    1536    1998    2502 
   Size:        897      18      80     541     462     504

  Fragments arranged by size: 

                 897     541     504     462      80      18
 
ApaI G_GGCC'C

Cuts at:      0      97    2165    2502 
   Size:         97    2068     337

  Fragments arranged by size: 

                2068     337      97
 
ApoI r'AATT_y

Cuts at:      0     168     637    1901    2014    2502 
   Size:        168     469    1264     113     488

  Fragments arranged by size: 

                1264     488     469     168     113
 
AseI AT'TA_AT

Cuts at:      0    1388    2502 
   Size:       1388    1114
 
AvaI C'yCGr_G

Cuts at:      0    1559    1616    2502 
   Size:       1559      57     886

  Fragments arranged by size: 

                1559     886      57
 
BanII G_rGCy'C

Cuts at:      0      97     755     851    2048    2165    2502 
   Size:         97     658      96    1197     117     337

  Fragments arranged by size: 

                1197     658     337     117      97      96
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     605    1230    2208    2502 
   Size:        605     625     978     294

  Fragments arranged by size: 

                 978     625     605     294
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2224    2258    2502 
   Size:       2224      34     244

  Fragments arranged by size: 

                2224     244      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     169    2502 
   Size:        169    2333
 
BclI T'GATC_A

Cuts at:      0    1820    2502 
   Size:       1820     682
 
BfrBI ATG'CAT

Cuts at:      0    1462    2502 
   Size:       1462    1040
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2160    2502 
   Size:       2160     342
 
BlpI GC'TnA_GC

Cuts at:      0     754    2502 
   Size:        754    1748
 
Bme1580I G_kGCm'C

Cuts at:      0      97    1029    1443    1710    1724    2165    2502 
   Size:         97     932     414     267      14     441     337

  Fragments arranged by size: 

                 932     441     414     337     267      97      14
 
BmgBI CAC'GTC

Cuts at:      0     826    2502 
   Size:        826    1676
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      79     285    1235    2502 
   Size:         79     206     950    1267

  Fragments arranged by size: 

                1267     950     206      79
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     725    2502 
   Size:        725    1777
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     439    1294    2502 
   Size:        247     192     855    1208

  Fragments arranged by size: 

                1208     855     247     192
 
Bpu10I CC'TnA_GC

Cuts at:      0     808    2502 
   Size:        808    1694
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238    1617    2502 
   Size:        238    1379     885

  Fragments arranged by size: 

                1379     885     238
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      39    2182    2325    2502 
   Size:         39    2143     143     177

  Fragments arranged by size: 

                2143     177     143      39
 
BsaAI yAC'GTr

Cuts at:      0    2086    2502 
   Size:       2086     416
 
BsaHI Gr'CG_yC

Cuts at:      0     470     866    1589    1886    2502 
   Size:        470     396     723     297     616

  Fragments arranged by size: 

                 723     616     470     396     297
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     422     452    1561    1591    2502 
   Size:        422      30    1109      30     911

  Fragments arranged by size: 

                1109     911     422      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      51     124     127     139     142     145     148     520 
   Size:         51      73       3      12       3       3       3     372

Cuts at:    520    1432    1876    2502 
   Size:        912     444     626

  Fragments arranged by size: 

                 912     626     444     372      73      51      12       3
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0      73    1467    2281    2502 
   Size:         73    1394     814     221

  Fragments arranged by size: 

                1394     814     221      73
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1096    1411    1462    1973    2191    2502 
   Size:       1096     315      51     511     218     311

  Fragments arranged by size: 

                1096     511     315     311     218      51
 
BsiHKAI G_wGCw'C

Cuts at:      0     365     546     851    2069    2502 
   Size:        365     181     305    1218     433

  Fragments arranged by size: 

                1218     433     365     305     181
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1495    2502 
   Size:       1495    1007
 
Bsp1286I G_dGCh'C

Cuts at:      0      97     365     546     755     851    1029    1443    1710 
   Size:         97     268     181     209      96     178     414     267

Cuts at:   1710    1724    2048    2069    2165    2502 
   Size:         14     324      21      96     337

  Fragments arranged by size: 

                 414     337     324     268     267     209     181     178
                  97      96      96      21      14
 
BspHI T'CATG_A

Cuts at:      0     834    1943    2462    2502 
   Size:        834    1109     519      40

  Fragments arranged by size: 

                1109     834     519      40
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     990    1118    1484    2502 
   Size:        990     128     366    1018

  Fragments arranged by size: 

                1018     990     366     128
 
BsrBI CCG'CTC

Cuts at:      0    1052    2502 
   Size:       1052    1450
 
BsrDI GCAATG_nn'

Cuts at:      0     185    1085    1462    2502 
   Size:        185     900     377    1040

  Fragments arranged by size: 

                1040     900     377     185
 
BsrGI T'GTAC_A

Cuts at:      0     722    1019    2502 
   Size:        722     297    1483

  Fragments arranged by size: 

                1483     722     297
 
BssSI C'ACGA_G

Cuts at:      0     244    1541    2306    2502 
   Size:        244    1297     765     196

  Fragments arranged by size: 

                1297     765     244     196
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1455    2037    2502 
   Size:       1455     582     465

  Fragments arranged by size: 

                1455     582     465
 
BstYI r'GATC_y

Cuts at:      0     146    1197    1487    2436    2502 
   Size:        146    1051     290     949      66

  Fragments arranged by size: 

                1051     949     290     146      66
 
Bsu36I CC'TnA_GG

Cuts at:      0       9    1036    2502 
   Size:          9    1027    1466

  Fragments arranged by size: 

                1466    1027       9
 
BtgI C'CryG_G

Cuts at:      0    1361    2183    2502 
   Size:       1361     822     319

  Fragments arranged by size: 

                1361     822     319
 
BtsI GCAGTG_nn'

Cuts at:      0    1311    1479    1979    1985    2343    2502 
   Size:       1311     168     500       6     358     159

  Fragments arranged by size: 

                1311     500     358     168     159       6
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     607    2502 
   Size:        607    1895
 
EaeI y'GGCC_r

Cuts at:      0     140     731    2057    2114    2502 
   Size:        140     591    1326      57     388

  Fragments arranged by size: 

                1326     591     388     140      57
 
EarI CTCTTCn'nnn_

Cuts at:      0     502     510    1786    2502 
   Size:        502       8    1276     716

  Fragments arranged by size: 

                1276     716     502       8
 
EcoICRI GAG'CTC

Cuts at:      0     849    2502 
   Size:        849    1653
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     439     580     763    1294    2502 
   Size:        247     192     141     183     531    1208

  Fragments arranged by size: 

                1208     531     247     192     183     141
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1252    1807    1957    2502 
   Size:       1252     555     150     545

  Fragments arranged by size: 

                1252     555     545     150
 
EcoO109I rG'GnC_Cy

Cuts at:      0      94    1473    2161    2162    2502 
   Size:         94    1379     688       1     340

  Fragments arranged by size: 

                1379     688     340      94       1
 
EcoRI G'AATT_C

Cuts at:      0     168    1901    2502 
   Size:        168    1733     601

  Fragments arranged by size: 

                1733     601     168
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     371     403     903     935    1535    1561    1567    1593 
   Size:        371      32     500      32     600      26       6      26

Cuts at:   1593    2502 
   Size:        909

  Fragments arranged by size: 

                 909     600     500     371      32      32      26      26
                   6
 
HincII GTy'rAC

Cuts at:      0     647    2502 
   Size:        647    1855
 
HindIII A'AGCT_T

Cuts at:      0     256     346    2296    2502 
   Size:        256      90    1950     206

  Fragments arranged by size: 

                1950     256     206      90
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     853    2094    2502 
   Size:        853    1241     408

  Fragments arranged by size: 

                1241     853     408
 
MscI TGG'CCA

Cuts at:      0     733    2116    2502 
   Size:        733    1383     386

  Fragments arranged by size: 

                1383     733     386
 
MslI CAynn'nnrTG

Cuts at:      0     155    1828    1942    2110    2502 
   Size:        155    1673     114     168     392

  Fragments arranged by size: 

                1673     392     168     155     114
 
MspA1I CmG'CkG

Cuts at:      0    1549    2062    2272    2502 
   Size:       1549     513     210     230

  Fragments arranged by size: 

                1549     513     230     210
 
NcoI C'CATG_G

Cuts at:      0    1361    2183    2502 
   Size:       1361     822     319

  Fragments arranged by size: 

                1361     822     319
 
NsiI A_TGCA'T

Cuts at:      0    1464    2502 
   Size:       1464    1038
 
NspI r_CATG'y

Cuts at:      0    2099    2502 
   Size:       2099     403
 
PasI CC'CwG_GG

Cuts at:      0      98    1029    2502 
   Size:         98     931    1473

  Fragments arranged by size: 

                1473     931      98
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     897    1536    2502 
   Size:        897     639     966

  Fragments arranged by size: 

                 966     897     639
 
PfoI T'CCnGG_A

Cuts at:      0    1490    2502 
   Size:       1490    1012
 
PmlI CAC'GTG

Cuts at:      0    2086    2502 
   Size:       2086     416
 
PpuMI rG'GwC_Cy

Cuts at:      0    1473    2502 
   Size:       1473    1029
 
PspOMI G'GGCC_C

Cuts at:      0      93    2161    2502 
   Size:         93    2068     341

  Fragments arranged by size: 

                2068     341      93
 
PvuII CAG'CTG

Cuts at:      0    1549    2272    2502 
   Size:       1549     723     230

  Fragments arranged by size: 

                1549     723     230
 
SacI G_AGCT'C

Cuts at:      0     851    2502 
   Size:        851    1651
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1786    2502 
   Size:       1786     716
 
SexAI A'CCwGG_T

Cuts at:      0    1958    2502 
   Size:       1958     544
 
SfcI C'TryA_G

Cuts at:      0     688    1522    2502 
   Size:        688     834     980

  Fragments arranged by size: 

                 980     834     688
 
SmaI CCC'GGG

Cuts at:      0    1618    2502 
   Size:       1618     884
 
SmlI C'TyrA_G

Cuts at:      0     253    1632    2502 
   Size:        253    1379     870

  Fragments arranged by size: 

                1379     870     253
 
SpeI A'CTAG_T

Cuts at:      0     202    2502 
   Size:        202    2300
 
StyI C'CwwG_G

Cuts at:      0      22     983    1361    1448    1659    1926    2183    2502 
   Size:         22     961     378      87     211     267     257     319

  Fragments arranged by size: 

                 961     378     319     267     257     211      87      22
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      57    2502 
   Size:         57    2445
 
TatI w'GTAC_w

Cuts at:      0     722    1019    2502 
   Size:        722     297    1483

  Fragments arranged by size: 

                1483     722     297
 
XmaI C'CCGG_G

Cuts at:      0    1616    2502 
   Size:       1616     886
 
XmnI GAAnn'nnTTC

Cuts at:      0    2458    2502 
   Size:       2458      44
 
ZraI GAC'GTC

Cuts at:      0     867    1590    2502 
   Size:        867     723     912

  Fragments arranged by size: 

                 912     867     723
 

 Enzymes that do cut:

    AatII     AccI     AclI     AcuI   AflIII     AlfI    AlwNI     ApaI
     ApoI     AseI     AvaI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrGI    BssSI    BstXI    BstYI   Bsu36I     BtgI     BtsI     DrdI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    Hin4I
   HincII  HindIII     MmeI     MscI     MslI   MspA1I     NcoI     NsiI
     NspI     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI    PvuII
     SacI     SapI    SexAI     SfcI     SmaI     SmlI     SpeI     StyI
    TaqII     TatI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AfeI    AflII     AgeI     AhdI     AleI     AloI
    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI    BglII     BmtI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI
    BspEI    BsrFI   BssHII   BstAPI    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     EagI     EciI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PciI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
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