Restriction map for pF1KB5791
 (Linear) (Six-base) MAPSORT of: KIBB5791.seq  Check: 6765  from: 1  to: 2490



KIBB5791.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     553    1438    1798    2490 
   Size:        553     885     360     692

  Fragments arranged by size: 

                 885     692     553     360
 
Acc65I G'GTAC_C

Cuts at:      0     698    1406    2490 
   Size:        698     708    1084

  Fragments arranged by size: 

                1084     708     698
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     814     852    1271    2048    2154    2490 
   Size:         60     754      38     419     777     106     336

  Fragments arranged by size: 

                 777     754     419     336     106      60      38
 
AflIII A'CryG_T

Cuts at:      0     494     621    1603    2121    2490 
   Size:        494     127     982     518     369

  Fragments arranged by size: 

                 982     518     494     369     127
 
AleI CACnn'nnGTG

Cuts at:      0    1695    2490 
   Size:       1695     795
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1552    2490 
   Size:       1552     938
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     596     628    2490 
   Size:        596      32    1862

  Fragments arranged by size: 

                1862     596      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     424     978    2490 
   Size:        424     554    1512

  Fragments arranged by size: 

                1512     554     424
 
ApaI G_GGCC'C

Cuts at:      0     716    1832    2490 
   Size:        716    1116     658

  Fragments arranged by size: 

                1116     716     658
 
ApoI r'AATT_y

Cuts at:      0      56    1051    1494    1771    2490 
   Size:         56     995     443     277     719

  Fragments arranged by size: 

                 995     719     443     277      56
 
AvaI C'yCGr_G

Cuts at:      0     523     924    1370    2481    2490 
   Size:        523     401     446    1111       9

  Fragments arranged by size: 

                1111     523     446     401       9
 
AvrII C'CTAG_G

Cuts at:      0    2442    2490 
   Size:       2442      48
 
BamHI G'GATC_C

Cuts at:      0     278    2490 
   Size:        278    2212
 
BanI G'GyrC_C

Cuts at:      0     698    1396    1406    2490 
   Size:        698     698      10    1084

  Fragments arranged by size: 

                1084     698     698      10
 
BanII G_rGCy'C

Cuts at:      0     614     716    1832    2490 
   Size:        614     102    1116     658

  Fragments arranged by size: 

                1116     658     614     102
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1067    2490 
   Size:       1067    1423
 
BlpI GC'TnA_GC

Cuts at:      0    1540    2490 
   Size:       1540     950
 
Bme1580I G_kGCm'C

Cuts at:      0      84     716     787    1717    1832    2490 
   Size:         84     632      71     930     115     658

  Fragments arranged by size: 

                 930     658     632     115      84      71
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     357     774     943    1315    1348    2490 
   Size:        357     417     169     372      33    1142

  Fragments arranged by size: 

                1142     417     372     357     169      33
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     538    2490 
   Size:        538    1952
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1026    1186    1490    1843    1895    2072    2490 
   Size:       1026     160     304     353      52     177     418

  Fragments arranged by size: 

                1026     418     353     304     177     160      52
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628    1227    2490 
   Size:        628     599    1263

  Fragments arranged by size: 

                1263     628     599
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     520    2490 
   Size:        520    1970
 
BsaAI yAC'GTr

Cuts at:      0     588     624    2490 
   Size:        588      36    1866

  Fragments arranged by size: 

                1866     588      36
 
BsaBI GATnn'nnATC

Cuts at:      0     277     403    2490 
   Size:        277     126    2087

  Fragments arranged by size: 

                2087     277     126
 
BsaWI w'CCGG_w

Cuts at:      0     900    2490 
   Size:        900    1590
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     244     274     466     496     532     562     596     626 
   Size:        244      30     192      30      36      30      34      30

Cuts at:    626    1448    1478    2490 
   Size:        822      30    1012

  Fragments arranged by size: 

                1012     822     244     192      36      34      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1283    2490 
   Size:       1283    1207
 
BseYI C'CCAG_C

Cuts at:      0     715     942    2490 
   Size:        715     227    1548

  Fragments arranged by size: 

                1548     715     227
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     672     696     985    1109    1512    2213    2490 
   Size:        672      24     289     124     403     701     277

  Fragments arranged by size: 

                 701     672     403     289     277     124      24
 
BsiEI CG_ry'CG

Cuts at:      0    1669    2490 
   Size:       1669     821
 
BsiHKAI G_wGCw'C

Cuts at:      0     446     614     629    2490 
   Size:        446     168      15    1861

  Fragments arranged by size: 

                1861     446     168      15
 
BsmI GAATG_Cn'

Cuts at:      0     733     841    1516    1849    2490 
   Size:        733     108     675     333     641

  Fragments arranged by size: 

                 733     675     641     333     108
 
Bsp1286I G_dGCh'C

Cuts at:      0      84     446     614     629     716     787    1717    1832 
   Size:         84     362     168      15      87      71     930     115

Cuts at:   1832    2490 
   Size:        658

  Fragments arranged by size: 

                 930     658     362     168     115      87      84      71
                  15
 
BspHI T'CATG_A

Cuts at:      0     629    2490 
   Size:        629    1861
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     553     829    1438    1798    1891    2287    2490 
   Size:        553     276     609     360      93     396     203

  Fragments arranged by size: 

                 609     553     396     360     276     203      93
 
BsrBI CCG'CTC

Cuts at:      0    1020    2332    2490 
   Size:       1020    1312     158

  Fragments arranged by size: 

                1312    1020     158
 
BsrDI GCAATG_nn'

Cuts at:      0     919    1440    1927    2490 
   Size:        919     521     487     563

  Fragments arranged by size: 

                 919     563     521     487
 
BssSI C'ACGA_G

Cuts at:      0    1763    2490 
   Size:       1763     727
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1193    2490 
   Size:       1193    1297
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     709     723    1839    2251    2490 
   Size:        709      14    1116     412     239

  Fragments arranged by size: 

                1116     709     412     239      14
 
BstYI r'GATC_y

Cuts at:      0     278    1347    2490 
   Size:        278    1069    1143

  Fragments arranged by size: 

                1143    1069     278
 
BtgI C'CryG_G

Cuts at:      0    1001    1179    2490 
   Size:       1001     178    1311

  Fragments arranged by size: 

                1311    1001     178
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2155    2490 
   Size:       2155     335
 
BtsI GCAGTG_nn'

Cuts at:      0     444     679     684     973    1022    1345    2490 
   Size:        444     235       5     289      49     323    1145

  Fragments arranged by size: 

                1145     444     323     289     235      49       5
 
EaeI y'GGCC_r

Cuts at:      0       2     203    2253    2490 
   Size:          2     201    2050     237

  Fragments arranged by size: 

                2050     237     201       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     872    2490 
   Size:        872    1618
 
EcoICRI GAG'CTC

Cuts at:      0     612    2490 
   Size:        612    1878
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     814     852    1026    1186    1271    1490    1843 
   Size:         60     754      38     174     160      85     219     353

Cuts at:   1843    1895    2048    2072    2154    2490 
   Size:         52     153      24      82     336

  Fragments arranged by size: 

                 754     353     336     219     174     160     153      85
                  82      60      52      38      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     238     806    1910    2490 
   Size:        238     568    1104     580

  Fragments arranged by size: 

                1104     580     568     238
 
EcoO109I rG'GnC_Cy

Cuts at:      0     243    1828    2490 
   Size:        243    1585     662

  Fragments arranged by size: 

                1585     662     243
 
EcoRI G'AATT_C

Cuts at:      0    1494    1771    2490 
   Size:       1494     277     719

  Fragments arranged by size: 

                1494     719     277
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2015    2047    2490 
   Size:       2015      32     443

  Fragments arranged by size: 

                2015     443      32
 
HincII GTy'rAC

Cuts at:      0    2264    2490 
   Size:       2264     226
 
HindIII A'AGCT_T

Cuts at:      0    1331    2490 
   Size:       1331    1159
 
KpnI G_GTAC'C

Cuts at:      0     702    1410    2490 
   Size:        702     708    1080

  Fragments arranged by size: 

                1080     708     702
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     876    2177    2490 
   Size:        876    1301     313

  Fragments arranged by size: 

                1301     876     313
 
MscI TGG'CCA

Cuts at:      0       4     205    2255    2490 
   Size:          4     201    2050     235

  Fragments arranged by size: 

                2050     235     201       4
 
MslI CAynn'nnrTG

Cuts at:      0     153     303    1695    2490 
   Size:        153     150    1392     795

  Fragments arranged by size: 

                1392     795     153     150
 
MspA1I CmG'CkG

Cuts at:      0    1046    1100    1164    1244    2054    2490 
   Size:       1046      54      64      80     810     436

  Fragments arranged by size: 

                1046     810     436      80      64      54
 
NcoI C'CATG_G

Cuts at:      0    1001    2490 
   Size:       1001    1489
 
NdeI CA'TA_TG

Cuts at:      0    2451    2490 
   Size:       2451      39
 
NspI r_CATG'y

Cuts at:      0     498    1095    1607    2096    2125    2243    2490 
   Size:        498     597     512     489      29     118     247

  Fragments arranged by size: 

                 597     512     498     489     247     118      29
 
PciI A'CATG_T

Cuts at:      0     494    1603    2121    2490 
   Size:        494    1109     518     369

  Fragments arranged by size: 

                1109     518     494     369
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      45     648    2159    2490 
   Size:         45     603    1511     331

  Fragments arranged by size: 

                1511     603     331      45
 
PfoI T'CCnGG_A

Cuts at:      0    1974    2303    2490 
   Size:       1974     329     187

  Fragments arranged by size: 

                1974     329     187
 
PmlI CAC'GTG

Cuts at:      0     624    2490 
   Size:        624    1866
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     596     628    2490 
   Size:        596      32    1862

  Fragments arranged by size: 

                1862     596      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     243    2490 
   Size:        243    2247
 
PshAI GACnn'nnGTC

Cuts at:      0    1119    2483    2490 
   Size:       1119    1364       7

  Fragments arranged by size: 

                1364    1119       7
 
PsiI TTA'TAA

Cuts at:      0    1595    2490 
   Size:       1595     895
 
PspOMI G'GGCC_C

Cuts at:      0     712    1828    2490 
   Size:        712    1116     662

  Fragments arranged by size: 

                1116     712     662
 
PstI C_TGCA'G

Cuts at:      0     505     640     865    1423    2059    2490 
   Size:        505     135     225     558     636     431

  Fragments arranged by size: 

                 636     558     505     431     225     135
 
PvuI CG_AT'CG

Cuts at:      0    1669    2490 
   Size:       1669     821
 
PvuII CAG'CTG

Cuts at:      0    1046    2054    2490 
   Size:       1046    1008     436

  Fragments arranged by size: 

                1046    1008     436
 
SacI G_AGCT'C

Cuts at:      0     614    2490 
   Size:        614    1876
 
SfcI C'TryA_G

Cuts at:      0     255     501     636     861    1071    1233    1419    1468 
   Size:        255     246     135     225     210     162     186      49

Cuts at:   1468    1962    2055    2490 
   Size:        494      93     435

  Fragments arranged by size: 

                 494     435     255     246     225     210     186     162
                 135      93      49
 
SmaI CCC'GGG

Cuts at:      0    1372    2490 
   Size:       1372    1118
 
SmlI C'TyrA_G

Cuts at:      0     523     607    1206    2490 
   Size:        523      84     599    1284

  Fragments arranged by size: 

                1284     599     523      84
 
SphI G_CATG'C

Cuts at:      0    2096    2243    2490 
   Size:       2096     147     247

  Fragments arranged by size: 

                2096     247     147
 
SspI AAT'ATT

Cuts at:      0     162    2490 
   Size:        162    2328
 
StyI C'CwwG_G

Cuts at:      0    1001    2442    2490 
   Size:       1001    1441      48

  Fragments arranged by size: 

                1441    1001      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     297    2350    2490 
   Size:        297    2053     140

  Fragments arranged by size: 

                2053     297     140
 
TatI w'GTAC_w

Cuts at:      0    1660    1958    2490 
   Size:       1660     298     532

  Fragments arranged by size: 

                1660     532     298
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     890     922    1448    1480    2490 
   Size:        890      32     526      32    1010

  Fragments arranged by size: 

                1010     890     526      32      32
 
XbaI T'CTAG_A

Cuts at:      0    2202    2490 
   Size:       2202     288
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2218    2490 
   Size:       2218     272
 
XhoI C'TCGA_G

Cuts at:      0     523    2490 
   Size:        523    1967
 
XmaI C'CCGG_G

Cuts at:      0    1370    2490 
   Size:       1370    1120
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AleI     AlfI     AloI    AlwNI
     ApaI     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BglI
     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BssSI   BstAPI
    BstXI    BstYI     BtgI    BtgZI     BtsI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    Hin4I   HincII  HindIII     KpnI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NspI     PciI
    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PstI     PvuI    PvuII     SacI     SfcI     SmaI     SmlI     SphI
     SspI     StyI    TaqII     TatI     TstI     XbaI     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI    ApaLI
     AscI     AseI    AsiSI     BaeI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI   Bpu10I    BsaHI
    BsiWI    BsmBI    BspEI    BsrFI    BsrGI   BssHII    BstBI   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI
     KasI     MfeI     MluI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PmeI    PspXI     PsrI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     StuI     SwaI
  Tth111I     XmnI     ZraI
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