Restriction map for pF1KB5901
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflIII      AgeI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        AvaI        AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BcgI        BclI        
BcnI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrFI       BssHII      
BssSI       BstBI       BstEII      BstPI       BstXI       
BstYI       CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        EagI        EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRV       
EcoT14I     FbaI        FseI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MunI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        StuI        
StyI        SwaI        Tth111I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI         1011  GTATAC               GT^[AC][GT]AC
AflII         868  CTTAAG               C^TTAAG
AhdI          734  GACAAACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         758  CAGTTACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          545  CCTCAGG              CC^T[ACGT]AGG
ApaI         1032  GGGCCC               GGGCC^C
AspEI         734  GACAAACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         753  GTGCTC               G[AT]GC[AT]^C
AviII          39  TGCGCA               TGC^GCA
BanI         1401  GGTGCC               G^G[CT][GA]CC
BbvCI         468  CCTCAGC              CCTCAGC(-5/-2)
BfaI           95  CTAG                 C^TAG
BfrI          868  CTTAAG               C^TTAAG
BseRI          28  GAGGAG               GAGGAG(10/8)
BsgI         1364  GTGCAG               GTGCAG(16/14)
BsiHKAI       753  GTGCTC               G[AT]GC[AT]^C
BsmAI         288  GTCTC                GTCTC(1/5)
BsmI         1228  GCATTC               GAATGC(1/-1)
Bsp1407I     1181  TGTACA               T^GTACA
BsrBI          70  GAGCGG               CCGCTC(-3/-3)
BsrGI        1181  TGTACA               T^GTACA
Bst1107I     1011  GTATAC               GTA^TAC
BstAPI        157  GCAAAAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      1011  GTATAC               GTA^TAC
Bsu36I        545  CCTCAGG              CC^T[ACGT]AGG
DraI          117  TTTAAA               TTT^AAA
DraII        1032  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EaeI         1351  CGGCCA               [CT]^GGCC[GA]
Eam1105I      734  GACAAACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        545  CCTCAGG              CC^T[ACGT]AGG
EcoO109I     1032  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         700  GAATTC               G^AATTC
EcoT22I       537  ATGCAT               ATGCA^T
FauI           22  CCCGC                CCCGC(4/6)
FspI           39  TGCGCA               TGC^GCA
HaeII         973  GGCGCT               [GA]GCGC^[CT]
HapII        1254  CCGG                 C^CGG
HpaII        1254  CCGG                 C^CGG
HphI           67  GGTGA                GGTGA(8/7)
MaeI           95  CTAG                 C^TAG
MaeII        1100  ACGT                 A^CGT
MspI         1254  CCGG                 C^CGG
NdeI          176  CATATG               CA^TATG
NsiI          537  ATGCAT               ATGCA^T
PflMI          49  CCATCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI          141  AGTACT               AGT^ACT
SfaNI         823  GATGC                GCATC(5/9)
SfcI         1481  CTACAG               C^T[GA][CT]AG
SphI           42  GCATGC               GCATG^C
SspBI        1181  TGTACA               T^GTACA
Van91I         49  CCATCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BanII          15  GGGCTC               G[GA]GC[CT]^C
             1032  GGGCCC              
BciVI         873  GGATAC               GTATCC(6/5)
             1495  GTATCC              
BsmFI        1097  GGGAC                GGGAC(10/14)
             1213  GTCCC               
CfoI           40  GCGC                 GCG^C
              974  GCGC                
DsaI           23  CCGCGG               C^C[GA][CT]GG
             1348  CCGCGG              
HhaI           40  GCGC                 GCG^C
              974  GCGC                
HinP1I         40  GCGC                 G^CGC
              974  GCGC                
KspI           23  CCGCGG               CCGC^GG
             1348  CCGCGG              
MspA1I         23  CCGCGG               C[AC]G^C[GT]G
             1348  CCGCGG              
NspI           42  GCATGC               [GA]CATG^[CT]
              609  GCATGT              
PleI          394  GACTC                GAGTC(4/5)
              286  GAGTC               
SacII          23  CCGCGG               CCGC^GG
             1348  CCGCGG              
SmlI          868  CTTAAG               C^T[CT][GA]AG
              902  CTCAAG              
TaqI          573  TCGA                 T^CGA
             1383  TCGA                
TthHB8I       573  TCGA                 T^CGA
             1383  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AvaII       BbvI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com