Restriction map for pF1KB5782
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AflIII      
AgeI        AhdI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BalI        BbvCI       BciVI       BclI        BfrI        
BglII       BlnI        BsaBI       BsiWI       BsmBI       
BspDI       BspLU11I    BsrBI       BssHII      BssSI       
Bst1107I    BstZ17I     ClaI        CpoI        DraI        
DrdI        Eam1105I    EarI        EcoRV       FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     MamI        MfeI        
MluI        MluNI       MscI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspI        PacI        PaeR7I      PinAI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SacI        
SalI        SapI        SbfI        SfiI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TfiI        XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

AatII        1567  GACGTC               GACGT^C
AccIII        679  TCCGGA               T^CCGGA
AcsI          892  GAATTC               [GA]^AATT[CT]
Alw44I         72  GTGCAC               G^TGCAC
AocI          426  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1625  AGCGCT               AGC^GCT
ApaLI          72  GTGCAC               G^TGCAC
ApoI          892  GAATTC               [GA]^AATT[CT]
AspHI          72  GTGCAC               G[AT]GC[AT]^C
AspI         1531  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         165  GGATCC               G^GATCC
BbeI          826  GGCGCC               GGCGC^C
BbrPI         616  CACGTG               CAC^GTG
BfaI          445  CTAG                 C^TAG
BlpI         1709  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1709  GCTCAGC              GC^T[ACGT]AGC
BsaAI         616  CACGTG               [CT]AC^GT[GA]
BsaI         1361  GAGACC               GGTCTC(1/5)
BsaWI         679  TCCGGA               [AT]^CCGG[AT]
BseAI         679  TCCGGA               T^CCGGA
BsiHKAI        72  GTGCAC               G[AT]GC[AT]^C
BsmAI        1361  GAGAC                GTCTC(1/5)
Bsp1407I      875  TGTACA               T^GTACA
BspEI         679  TCCGGA               T^CCGGA
BsrGI         875  TGTACA               T^GTACA
BstBI        1900  TTCGAA               TT^CGAA
BstEII        963  GGTGACC              G^GT[ACGT]ACC
BstPI         963  GGTGACC              G^GT[ACGT]ACC
BstYI         165  GGATCC               [GA]^GATC[CT]
Bsu36I        426  CCTCAGG              CC^T[ACGT]AGG
CelII        1709  GCTCAGC              GC^T[ACGT]AGC
Eco47III     1625  AGCGCT               AGC^GCT
Eco81I        426  CCTCAGG              CC^T[ACGT]AGG
EcoNI          60  CCTCTCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       963  GGTGACC              G^GT[ACGT]ACC
EcoRI         892  GAATTC               G^AATTC
EcoT22I       105  ATGCAT               ATGCA^T
KasI          826  GGCGCC               G^GCGCC
KspI         1753  CCGCGG               CCGC^GG
MaeI          445  CTAG                 C^TAG
MflI          165  GGATCC               [GA]^GATC[CT]
MroI          679  TCCGGA               T^CCGGA
MseI         1067  TTAA                 T^TAA
MslI         2058  CATCTATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          826  GGCGCC               GG^CGCC
NcoI         1377  CCATGG               C^CATGG
NsiI          105  ATGCAT               ATGCA^T
NspV         1900  TTCGAA               TT^CGAA
PflFI        1531  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1470  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         616  CACGTG               CAC^GTG
PmlI          616  CACGTG               CAC^GTG
PshAI         654  GACCGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII        1753  CCGCGG               CCGC^GG
ScaI         1595  AGTACT               AGT^ACT
SexAI         130  ACCTGGT              A^CC[AT]GGT
SfoI          826  GGCGCC               GGC^GCC
SfuI         1900  TTCGAA               TT^CGAA
SmlI         2227  CTCAAG               C^T[CT][GA]AG
SspBI         875  TGTACA               T^GTACA
Tru9I        1067  TTAA                 T^TAA
Tsp509I       893  AATT                 ^AATT
Tth111I      1531  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1470  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         165  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BseRI        1089  CTCCTC               GAGGAG(10/8)
              882  GAGGAG              
BsiEI         489  CGGCCG               CG[GA][CT]^CG
              849  CGGCCG              
BspHI         206  TCATGA               T^CATGA
             2282  TCATGA              
BsrDI        1049  GCAATG               GCAATG(2/0)
             1160  GCAATG              
BsrFI        1340  GCCGGT               [GA]^CCGG[CT]
             1781  GCCGGT              
BstXI         968  CCAGTAGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1046  CCAGCAATGTGG        
Cfr10I       1340  GCCGGT               [GA]^CCGG[CT]
             1781  GCCGGT              
EagI          489  CGGCCG               C^GGCCG
              849  CGGCCG              
EclXI         489  CGGCCG               C^GGCCG
              849  CGGCCG              
Eco52I        489  CGGCCG               C^GGCCG
              849  CGGCCG              
EcoT14I       365  CCAAGG               C^C[AT][AT]GG
             1377  CCATGG              
RcaI          206  TCATGA               T^CATGA
             2282  TCATGA              
SmaI         1653  CCCGGG               CCC^GGG
             1695  CCCGGG              
StyI          365  CCAAGG               C^C[AT][AT]GG
             1377  CCATGG              
XcmI         1013  CCAAGTCAGCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1094  CCAGGGTCTTCCTGG     
XmaCI        1653  CCCGGG               C^CCGGG
             1695  CCCGGG              
XmaI         1653  CCCGGG               C^CCGGG
             1695  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AvaI        AvaII       
BanI        BanII       BbsI        BbvI        BcgI        
BcnI        BglI        BmyI        BpmI        BpuAI       
BsaHI       BsaJI       BsgI        BsiYI       BslI        
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
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