Restriction map for pF1KB5736
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

Acc65I      AccIII      AclI        AflIII      AgeI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BclI        
BglII       BlnI        BlpI        Bpu1102I    BsaWI       
BseAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     CelII       
ClaI        CpoI        DraI        DrdI        Eco47III    
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HpaI        KpnI        MluI        MroI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SapI        SbfI        SfiI        SfuI        SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII         747  GACGTC               GACGT^C
AccI         1779  GTCGAC               GT^[AC][GT]AC
AcsI          209  AAATTT               [GA]^AATT[CT]
AflII         697  CTTAAG               C^TTAAG
Alw44I       1021  GTGCAC               G^TGCAC
AlwNI        1298  CAGAGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1075  CCTCAGG              CC^T[ACGT]AGG
ApaLI        1021  GTGCAC               G^TGCAC
ApoI          209  AAATTT               [GA]^AATT[CT]
BamHI        1065  GGATCC               G^GATCC
BbrPI        1393  CACGTG               CAC^GTG
BbsI         1510  GTCTTC               GAAGAC(2/6)
BbvCI        1008  GCTGAGG              CCTCAGC(-5/-2)
BcgI          110  GCATCACCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          697  CTTAAG               C^TTAAG
BglI         1877  GCCGAGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        1510  GTCTTC               GAAGAC(2/6)
BsaAI        1393  CACGTG               [CT]AC^GT[GA]
BsaBI        1338  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         2086  GTGCAG               GTGCAG(16/14)
BsiEI         917  CGGCCG               CG[GA][CT]^CG
BsrDI         857  GCAATG               GCAATG(2/0)
BsrFI         707  ACCGGC               [GA]^CCGG[CT]
BstEII       1275  GGTGACC              G^GT[ACGT]ACC
BstPI        1275  GGTGACC              G^GT[ACGT]ACC
Bsu36I       1075  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        707  ACCGGC               [GA]^CCGG[CT]
DraIII       1784  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          917  CGGCCG               C^GGCCG
EarI           67  GAAGAG               CTCTTC(1/4)
EclXI         917  CGGCCG               C^GGCCG
Eco52I        917  CGGCCG               C^GGCCG
Eco81I       1075  CCTCAGG              CC^T[ACGT]AGG
EcoNI          57  CCTGGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1275  GGTGACC              G^GT[ACGT]ACC
HindIII      2157  AAGCTT               A^AGCTT
Ksp632I        67  GAAGAG               CTCTTC(1/4)
MamI         1338  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1118  CAATTG               C^AATTG
MseI          698  TTAA                 T^TAA
MunI         1118  CAATTG               C^AATTG
PmaCI        1393  CACGTG               CAC^GTG
PmlI         1393  CACGTG               CAC^GTG
PstI          139  CTGCAG               CTGCA^G
SacI          579  GAGCTC               GAGCT^C
SalI         1779  GTCGAC               G^TCGAC
ScaI          161  AGTACT               AGT^ACT
SexAI          80  ACCTGGT              A^CC[AT]GGT
SgrAI         706  CACCGGCG             C[GA]^CCGG[CT]G
TaqI         1780  TCGA                 T^CGA
Tru9I         698  TTAA                 T^TAA
TthHB8I      1780  TCGA                 T^CGA
XcmI          528  CCACAGGACTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         150  CGCG                 CG^CG
             1149  CGCG                
AhdI         1307  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1466  GACACACCGTC         
AspEI        1307  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1466  GACACACCGTC         
BbeI          154  GGCGCC               GGCGC^C
              330  GGCGCC              
BfaI         1546  CTAG                 C^TAG
             1919  CTAG                
BsmBI          37  GAGACG               CGTCTC(1/5)
              869  GAGACG              
BspMI         653  GCAGGT               ACCTGC(4/8)
              954  GCAGGT              
BstUI         150  CGCG                 CG^CG
             1149  CGCG                
BstYI          89  AGATCC               [GA]^GATC[CT]
             1065  GGATCC              
Eam1105I     1307  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1466  GACACACCGTC         
KasI          154  GGCGCC               G^GCGCC
              330  GGCGCC              
KspI          149  CCGCGG               CCGC^GG
             1148  CCGCGG              
MaeI         1546  CTAG                 C^TAG
             1919  CTAG                
MflI           89  AGATCC               [GA]^GATC[CT]
             1065  GGATCC              
MvnI          150  CGCG                 CG^CG
             1149  CGCG                
NarI          154  GGCGCC               GG^CGCC
              330  GGCGCC              
NcoI         1286  CCATGG               C^CATGG
             2171  CCATGG              
NspI          944  ACATGC               [GA]CATG^[CT]
             1325  GCATGT              
SacII         149  CCGCGG               CCGC^GG
             1148  CCGCGG              
SfcI          139  CTGCAG               C^T[GA][CT]AG
             1680  CTACAG              
SfoI          154  GGCGCC               GGC^GCC
              330  GGCGCC              
TfiI          435  GAATC                G^A[AT]TC
              772  GATTC               
Tsp509I       210  AATT                 ^AATT
             1119  AATT                
XhoII          89  AGATCC               [GA]^GATC[CT]
             1065  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
ApaI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbvI        BciVI       BcnI        
BmyI        BpmI        BsaHI       BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspHI       BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        StuI        StyI        TseI        
Tsp45I      TspRI       Van91I      
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com