Restriction map for pF1KB5849
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BcgI        
BciVI       BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BseAI       BsgI        
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco52I      EcoNI       EcoO109I    EcoRI       
EcoRV       EcoT14I     EcoT22I     FseI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MaeII       MamI        
MfeI        MluI        MroI        MseI        MunI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StyI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AocI          430  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       635  AGCGCT               AGC^GCT
AviII         368  TGCGCA               TGC^GCA
BalI           44  TGGCCA               TGG^CCA
BanI          568  GGCACC               G^G[CT][GA]CC
BbvCI         253  GCTGAGG              CCTCAGC(-5/-2)
BclI          287  TGATCA               T^GATCA
BsaWI         302  ACCGGA               [AT]^CCGG[AT]
BsiEI         300  CGACCG               CG[GA][CT]^CG
BsmI          397  GAATGC               GAATGC(1/-1)
Bsp1407I      479  TGTACA               T^GTACA
BsrGI         479  TGTACA               T^GTACA
BstAPI         78  GCAGTGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        453  GGTAACC              G^GT[ACGT]ACC
BstNI         362  CCTGG                CC^[AT]GG
BstPI         453  GGTAACC              G^GT[ACGT]ACC
Bsu36I        430  CCTGAGG              CC^T[ACGT]AGG
EaeI           44  TGGCCA               [CT]^GGCC[GA]
Eco47III      635  AGCGCT               AGC^GCT
Eco81I        430  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       453  GGTAACC              G^GT[ACGT]ACC
EcoRII        362  CCTGG                ^CC[AT]GG
FbaI          287  TGATCA               T^GATCA
FspI          368  TGCGCA               TGC^GCA
HaeII         635  AGCGCT               [GA]GCGC^[CT]
HgaI          114  GCGTC                GACGC(5/10)
MluNI          44  TGGCCA               TGG^CCA
MscI           44  TGGCCA               TGG^CCA
MslI          279  CACATATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          362  CCTGG                CC^[AT]GG
NdeI          281  CATATG               CA^TATG
NspI          629  ACATGC               [GA]CATG^[CT]
SmaI          504  CCCGGG               CCC^GGG
SspBI         479  TGTACA               T^GTACA
StuI          668  AGGCCT               AGG^CCT
TaqI          299  TCGA                 T^CGA
Tsp45I         98  GTCAC                ^GT[GC]AC
TthHB8I       299  TCGA                 T^CGA
XmaCI         504  CCCGGG               C^CCGGG
XmaI          504  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccII         358  CGCG                 CG^CG
              388  CGCG                
AlwNI          79  CAGTGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              176  CAGCCACTG           
AvaI          504  CCCGGG               C^[CT]CG[GA]G
              595  CCCGAG              
BpmI           37  CTGGAG               CTGGAG(16/14)
               88  CTGGAG              
BseRI         228  GAGGAG               GAGGAG(10/8)
              439  GAGGAG              
BsoBI         504  CCCGGG               C^[CT]CG[GA]G
              595  CCCGAG              
BstUI         358  CGCG                 CG^CG
              388  CGCG                
BstYI         529  AGATCC               [GA]^GATC[CT]
              710  AGATCC              
EarI           93  GAAGAG               CTCTTC(1/4)
              225  GAAGAG              
HinfI          96  GAGTC                G^A[ACGT]TC
              468  GAGTC               
Ksp632I        93  GAAGAG               CTCTTC(1/4)
              225  GAAGAG              
MflI          529  AGATCC               [GA]^GATC[CT]
              710  AGATCC              
MspA1I        214  CAGCGG               C[AC]G^C[GT]G
              424  CCGCTG              
MvnI          358  CGCG                 CG^CG
              388  CGCG                
PleI           96  GAGTC                GAGTC(4/5)
              468  GAGTC               
PstI          525  CTGCAG               CTGCA^G
              550  CTGCAG              
SfcI          525  CTGCAG               C^T[GA][CT]AG
              550  CTGCAG              
XhoII         529  AGATCC               [GA]^GATC[CT]
              710  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BanII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MnlI        MspI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TseI        TspRI       
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