Restriction map for pF1KB9973
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BbrPI       BcgI        BciVI       BclI        
BglII       BlnI        BsaAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MroI        MslI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SwaI        TaqI        Tth111I     TthHB8I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          208  GTAGAC               GT^[AC][GT]AC
AccII           6  CGCG                 CG^CG
AcyI           13  GGCGCC               G[GA]^CG[CT]C
AflII         244  CTTAAG               C^TTAAG
ApaI         1110  GGGCCC               GGGCC^C
Asp700I       492  GAATATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         1128  TGGCCA               TGG^CCA
BbeI           13  GGCGCC               GGCGC^C
BbvCI        1236  CCTCAGC              CCTCAGC(-5/-2)
BfaI          593  CTAG                 C^TAG
BfrI          244  CTTAAG               C^TTAAG
BlpI         1261  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1261  GCTCAGC              GC^T[ACGT]AGC
BsaHI          13  GGCGCC               G[GA]^CG[CT]C
BsmI          529  GAATGC               GAATGC(1/-1)
BsrBI        1589  GAGCGG               CCGCTC(-3/-3)
BsrDI         892  GCAATG               GCAATG(2/0)
BsrFI        1074  GCCGGC               [GA]^CCGG[CT]
BssSI         183  CTCGTG               CACGAG(-5/-1)
BstEII        549  GGTGACC              G^GT[ACGT]ACC
BstPI         549  GGTGACC              G^GT[ACGT]ACC
BstUI           6  CGCG                 CG^CG
BstXI         120  CCACATGATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1261  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1074  GCCGGC               [GA]^CCGG[CT]
DraIII        724  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          641  CCATGG               C^C[GA][CT]GG
EcoO65I       549  GGTGACC              G^GT[ACGT]ACC
HgaI          401  GCGTC                GACGC(5/10)
Hin1I          13  GGCGCC               G[GA]^CG[CT]C
KasI           13  GGCGCC               G^GCGCC
MaeI          593  CTAG                 C^TAG
MluNI        1128  TGGCCA               TGG^CCA
MscI         1128  TGGCCA               TGG^CCA
MvnI            6  CGCG                 CG^CG
NaeI         1074  GCCGGC               GCC^GGC
NarI           13  GGCGCC               GG^CGCC
NcoI          641  CCATGG               C^CATGG
NgoMIV       1074  GCCGGC               G^CCGGC
NspI          995  GCATGC               [GA]CATG^[CT]
PpuMI         908  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         331  CAGCTG               CAG^CTG
ScaI          110  AGTACT               AGT^ACT
SfoI           13  GGCGCC               GGC^GCC
SmaI          865  CCCGGG               CCC^GGG
SmlI          244  CTTAAG               C^T[CT][GA]AG
SphI          995  GCATGC               GCATG^C
SspI          493  AATATT               AAT^ATT
XmaCI         865  CCCGGG               C^CCGGG
XmaI          865  CCCGGG               C^CCGGG
XmnI          492  GAATATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AspHI         765  GAGCAC               G[AT]GC[AT]^C
             1332  GAGCAC              
AvaI          865  CCCGGG               C^[CT]CG[GA]G
             1290  CTCGGG              
AvaII         909  GGTCC                G^G[AT]CC
             1372  GGACC               
BamHI         732  GGATCC               G^GATCC
             1459  GGATCC              
BanI           13  GGCGCC               G^G[CT][GA]CC
             1503  GGTGCC              
BbsI         1155  GAAGAC               GAAGAC(2/6)
             1489  GTCTTC              
BglI          646  GCCCCTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1226  GCCCCCAGGGC         
BpuAI        1155  GAAGAC               GAAGAC(2/6)
             1489  GTCTTC              
BsgI          721  CTGCAC               GTGCAG(16/14)
              328  GTGCAG              
BsiHKAI       765  GAGCAC               G[AT]GC[AT]^C
             1332  GAGCAC              
BsmFI         958  GGGAC                GGGAC(10/14)
              910  GTCCC               
BsoBI         865  CCCGGG               C^[CT]CG[GA]G
             1290  CTCGGG              
EarI          357  GAAGAG               CTCTTC(1/4)
              382  GAAGAG              
HaeII          13  GGCGCC               [GA]GCGC^[CT]
               23  GGCGCT              
Ksp632I       357  GAAGAG               CTCTTC(1/4)
              382  GAAGAG              
SapI          357  GAAGAGC              GCTCTTC(1/4)
              382  GAAGAGC             
SfaNI        1036  GCATC                GCATC(5/9)
             1054  GCATC               
StuI          395  AGGCCT               AGG^CCT
             1539  AGGCCT              
Tsp45I        550  GTGAC                ^GT[GC]AC
              599  GTGAC               
Tsp509I       192  AATT                 ^AATT
              904  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       Bsp1286I    BspMI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        StyI        TfiI        
Tru9I       TseI        TspRI       Van91I      XcmI        
XhoII       
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