Restriction map for pF1KB7973
 (Linear) (Six-base) MAPSORT of: KIBB7973.seq  Check: 5890  from: 1  to: 642



KIBB7973.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     540     642 
   Size:        187     353     102

  Fragments arranged by size: 

                 353     187     102
 
AflIII A'CryG_T

Cuts at:      0     467     642 
   Size:        467     175
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     478     510     642 
   Size:        478      32     132

  Fragments arranged by size: 

                 478     132      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     540     591     642 
   Size:        540      51      51

  Fragments arranged by size: 

                 540      51      51
 
AvaI C'yCGr_G

Cuts at:      0      22     642 
   Size:         22     620
 
BanI G'GyrC_C

Cuts at:      0      13     642 
   Size:         13     629
 
BbeI G_GCGC'C

Cuts at:      0      17     642 
   Size:         17     625
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     546     642 
   Size:        546      96
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     252     286     642 
   Size:        252      34     356

  Fragments arranged by size: 

                 356     252      34
 
BlpI GC'TnA_GC

Cuts at:      0     158     259     642 
   Size:        158     101     383

  Fragments arranged by size: 

                 383     158     101
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     450     642 
   Size:        450     192
 
BsaHI Gr'CG_yC

Cuts at:      0      14     176     611     642 
   Size:         14     162     435      31

  Fragments arranged by size: 

                 435     162      31      14
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     325     355     642 
   Size:        325      30     287

  Fragments arranged by size: 

                 325     287      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     183     346     349     583     642 
   Size:        183     163       3     234      59

  Fragments arranged by size: 

                 234     183     163      59       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     642 
   Size:        601      41
 
BsiHKAI G_wGCw'C

Cuts at:      0     575     642 
   Size:        575      67
 
Bsp1286I G_dGCh'C

Cuts at:      0     575     642 
   Size:        575      67
 
BspHI T'CATG_A

Cuts at:      0     377     642 
   Size:        377     265
 
BsrFI r'CCGG_y

Cuts at:      0     131     642 
   Size:        131     511
 
BssHII G'CGCG_C

Cuts at:      0     141     642 
   Size:        141     501
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     522     642 
   Size:        522     120
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      34     642 
   Size:         34     608
 
EaeI y'GGCC_r

Cuts at:      0     524     642 
   Size:        524     118
 
EarI CTCTTCn'nnn_

Cuts at:      0     160     642 
   Size:        160     482
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     540     642 
   Size:        187     353     102

  Fragments arranged by size: 

                 353     187     102
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27     642 
   Size:         27     615
 
HaeII r_GCGC'y

Cuts at:      0      17     642 
   Size:         17     625
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     325     357     430     462     642 
   Size:        325      32      73      32     180

  Fragments arranged by size: 

                 325     180      73      32      32
 
KasI G'GCGC_C

Cuts at:      0      13     642 
   Size:         13     629
 
MfeI C'AATT_G

Cuts at:      0     622     642 
   Size:        622      20
 
MscI TGG'CCA

Cuts at:      0     526     642 
   Size:        526     116
 
MslI CAynn'nnrTG

Cuts at:      0     382     520     642 
   Size:        382     138     122

  Fragments arranged by size: 

                 382     138     122
 
MspA1I CmG'CkG

Cuts at:      0     560     642 
   Size:        560      82
 
NarI GG'CG_CC

Cuts at:      0      14     642 
   Size:         14     628
 
PfoI T'CCnGG_A

Cuts at:      0     533     642 
   Size:        533     109
 
PpuMI rG'GwC_Cy

Cuts at:      0      27     642 
   Size:         27     615
 
PshAI GACnn'nnGTC

Cuts at:      0     211     642 
   Size:        211     431
 
PvuII CAG'CTG

Cuts at:      0     560     642 
   Size:        560      82
 
SexAI A'CCwGG_T

Cuts at:      0     440     452     642 
   Size:        440      12     190

  Fragments arranged by size: 

                 440     190      12
 
SfcI C'TryA_G

Cuts at:      0      54     642 
   Size:         54     588
 
SfoI GGC'GCC

Cuts at:      0      15     642 
   Size:         15     627
 
StuI AGG'CCT

Cuts at:      0     109     642 
   Size:        109     533
 
StyI C'CwwG_G

Cuts at:      0      31     642 
   Size:         31     611
 
Tth111I GACn'n_nGTC

Cuts at:      0     442     454     642 
   Size:        442      12     188

  Fragments arranged by size: 

                 442     188      12
 

 Enzymes that do cut:

     AcuI   AflIII     AloI    AlwNI     AvaI     BanI     BbeI     BbsI
     BcgI     BlpI     BsaI    BsaHI    BsaXI    BseRI     BsgI  BsiHKAI
 Bsp1286I    BspHI    BsrFI   BssHII    BstXI     DrdI     EaeI     EarI
  Eco57MI EcoO109I    HaeII    Hin4I     KasI     MfeI     MscI     MslI
   MspA1I     NarI     PfoI    PpuMI    PshAI    PvuII    SexAI     SfcI
     SfoI     StuI     StyI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII    BbvCI    BciVI     BclI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BseYI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MluI     MmeI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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