Restriction map for
pF1KB7958
(Circular) (Six-base) MAPSORT of: pF1KB7958.seq Check: 7052 from: 1 to: 4301
pF1KB7958 4301 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1339 1339
Size: 4301
Acc65I G'GTAC_C
Cuts at: 1317 1317
Size: 4301
AclI AA'CG_TT
Cuts at: 4141 4141
Size: 4301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 2558 3305 3573 2126
Size: 432 747 268 2854
Fragments arranged by size:
2854 747 432 268
AflII C'TTAA_G
Cuts at: 1096 1096
Size: 4301
AflIII A'CryG_T
Cuts at: 281 355 1653 2757 3734 281
Size: 74 1298 1104 977 848
Fragments arranged by size:
1298 1104 977 848 74
AgeI A'CCGG_T
Cuts at: 2689 2689
Size: 4301
AhdI GACnn_n'nnGTC
Cuts at: 627 627
Size: 4301
AleI CACnn'nnGTG
Cuts at: 236 949 236
Size: 713 3588
AlwNI CAG_nnn'CTG
Cuts at: 1172 1216 1606 3173 1172
Size: 44 390 1567 2300
Fragments arranged by size:
2300 1567 390 44
ApaLI G'TGCA_C
Cuts at: 3071 3071
Size: 4301
ApoI r'AATT_y
Cuts at: 994 1024 1140 1305 3654 3910 994
Size: 30 116 165 2349 256 1385
Fragments arranged by size:
2349 1385 256 165 116 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4301
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
AvaI C'yCGr_G
Cuts at: 1321 1321
Size: 4301
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 412 445 412
Size: 33 4268
BamHI G'GATC_C
Cuts at: 634 1326 634
Size: 692 3609
BanI G'GyrC_C
Cuts at: 1317 1731 1979 2014 1317
Size: 414 248 35 3604
Fragments arranged by size:
3604 414 248 35
BanII G_rGCy'C
Cuts at: 1315 2345 1315
Size: 1030 3271
BbeI G_GCGC'C
Cuts at: 1735 1983 1735
Size: 248 4053
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4267
BciVI GTATCCnnnnn_n'
Cuts at: 440 483 2199 2960 4301 440
Size: 43 1716 761 1341 440
Fragments arranged by size:
1716 1341 761 440 43
BclI T'GATC_A
Cuts at: 786 1821 786
Size: 1035 3266
BglII A'GATC_T
Cuts at: 1816 3853 1816
Size: 2037 2264
BlpI GC'TnA_GC
Cuts at: 1413 1413
Size: 4301
Bme1580I G_kGCm'C
Cuts at: 1926 2019 3075 1926
Size: 93 1056 3152
Fragments arranged by size:
3152 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1513 1594 1929 4073 1513
Size: 81 335 2144 1741
Fragments arranged by size:
2144 1741 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 542 1293 318
Size: 224 751 3326
Fragments arranged by size:
3326 751 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1477 2356 2848 3146 346
Size: 1131 879 492 298 1501
Fragments arranged by size:
1501 1131 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 695 4005 695
Size: 3310 991
BsaAI yAC'GTr
Cuts at: 1654 1654
Size: 4301
BsaBI GATnn'nnATC
Cuts at: 6 1815 3852 6
Size: 1809 2037 455
Fragments arranged by size:
2037 1809 455
BsaHI Gr'CG_yC
Cuts at: 1732 1980 2682 4178 1732
Size: 248 702 1496 1855
Fragments arranged by size:
1855 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1490 2011 2689 2963 3110 3521 1490
Size: 521 678 274 147 411 2270
Fragments arranged by size:
2270 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 869 899 1266 1296 869
Size: 30 367 30 3874
Fragments arranged by size:
3874 367 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 877 877
Size: 4301
BseYI C'CCAG_C
Cuts at: 111 1726 2484 3061 3557 111
Size: 1615 758 577 496 855
Fragments arranged by size:
1615 855 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1843 1889 3097 104
Size: 1739 46 1208 1308
Fragments arranged by size:
1739 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1315 2093 2283 3075 1315
Size: 778 190 792 2541
Fragments arranged by size:
2541 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1315 1926 2019 2093 2283 2345 3075 1315
Size: 611 93 74 190 62 730 2541
Fragments arranged by size:
2541 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3521 3521
Size: 4301
BspHI T'CATG_A
Cuts at: 435 3477 435
Size: 3042 1259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1351 1565 1867 2248 1351
Size: 214 302 381 3404
Fragments arranged by size:
3404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2593 2647 4131 4296 50
Size: 2543 54 1484 165 55
Fragments arranged by size:
2543 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1052 2213 1052
Size: 1161 3140
BsrFI r'CCGG_y
Cuts at: 2299 2480 2689 2299
Size: 181 209 3911
Fragments arranged by size:
3911 209 181
BsrGI T'GTAC_A
Cuts at: 420 420
Size: 4301
BssSI C'ACGA_G
Cuts at: 2572 2930 2572
Size: 358 3943
BstAPI GCAn_nnn'nTGC
Cuts at: 832 1172 1678 832
Size: 340 506 3455
Fragments arranged by size:
3455 506 340
BstBI TT'CG_AA
Cuts at: 2662 2662
Size: 4301
BstXI CCAn_nnnn'nTGG
Cuts at: 238 238
Size: 4301
BstYI r'GATC_y
Cuts at: 1 634 1326 1793 1816 2151 2397 3409 3495
Size: 633 692 467 23 335 246 1012 86
Cuts at: 3495 3507 3853 1
Size: 12 346 449
Fragments arranged by size:
1012 692 633 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 231 231
Size: 4301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2251 2432 2595 2251
Size: 181 163 3957
Fragments arranged by size:
3957 181 163
BtsI GCAGTG_nn'
Cuts at: 371 830 1287 1544 371
Size: 459 457 257 3128
Fragments arranged by size:
3128 459 457 257
DraI TTT'AAA
Cuts at: 1300 1300
Size: 4301
DrdI GACnn_nn'nnGTC
Cuts at: 2007 2865 2007
Size: 858 3443
EaeI y'GGCC_r
Cuts at: 1886 2060 2451 2478 3823 1886
Size: 174 391 27 1345 2364
Fragments arranged by size:
2364 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1886 1886
Size: 4301
EarI CTCTTCn'nnn_
Cuts at: 158 753 2324 2534 158
Size: 595 1571 210 1925
Fragments arranged by size:
1925 1571 595 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2509 2819 2965 4111 2509
Size: 310 146 1146 2699
Fragments arranged by size:
2699 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1313 1313
Size: 4301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 542 1293 2126 2558 3305 3573 318
Size: 224 751 833 432 747 268 1046
Fragments arranged by size:
1046 833 751 747 432 268 224
EcoNI CCTnn'n_nnAGG
Cuts at: 269 269
Size: 4301
EcoO109I rG'GnC_Cy
Cuts at: 147 601 1440 147
Size: 454 839 3008
Fragments arranged by size:
3008 839 454
EcoRI G'AATT_C
Cuts at: 1305 1305
Size: 4301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 631 2320 631
Size: 1689 2612
FspI TGC'GCA
Cuts at: 2082 2082
Size: 4301
HaeII r_GCGC'y
Cuts at: 1735 1983 3005 3984 1735
Size: 248 1022 979 2052
Fragments arranged by size:
2052 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 613 645 869 901 2143 2173 2175 2205 613
Size: 32 224 32 1242 30 2 30 2709
Fragments arranged by size:
2709 1242 224 32 32 30 30 2
HincII GTy'rAC
Cuts at: 176 1119 1340 176
Size: 943 221 3137
Fragments arranged by size:
3137 943 221
KasI G'GCGC_C
Cuts at: 1731 1979 1731
Size: 248 4053
KpnI G_GTAC'C
Cuts at: 1321 1321
Size: 4301
MluI A'CGCG_T
Cuts at: 3734 3734
Size: 4301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2972 3156 2972
Size: 184 4117
MscI TGG'CCA
Cuts at: 2062 2062
Size: 4301
MslI CAynn'nnrTG
Cuts at: 236 392 445 768 949 2290 2417 236
Size: 156 53 323 181 1341 127 2120
Fragments arranged by size:
2120 1341 323 181 156 127 53
MspA1I CmG'CkG
Cuts at: 1412 1726 2086 3099 3344 1412
Size: 314 360 1013 245 2369
Fragments arranged by size:
2369 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2482 2482
Size: 4301
NarI GG'CG_CC
Cuts at: 1732 1980 1732
Size: 248 4053
NdeI CA'TA_TG
Cuts at: 551 1041 551
Size: 490 3811
NgoMIV G'CCGG_C
Cuts at: 2480 2480
Size: 4301
NspI r_CATG'y
Cuts at: 285 359 450 1354 1854 2761 285
Size: 74 91 904 500 907 1825
Fragments arranged by size:
1825 907 904 500 91 74
PciI A'CATG_T
Cuts at: 281 355 2757 281
Size: 74 2402 1825
Fragments arranged by size:
2402 1825 74
PfoI T'CCnGG_A
Cuts at: 1145 1145
Size: 4301
PmeI GTTT'AAAC
Cuts at: 1300 1300
Size: 4301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 4269
PpuMI rG'GwC_Cy
Cuts at: 147 601 147
Size: 454 3847
PsiI TTA'TAA
Cuts at: 1109 1109
Size: 4301
PstI C_TGCA'G
Cuts at: 513 1018 1348 2033 513
Size: 505 330 685 2781
Fragments arranged by size:
2781 685 505 330
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4301
PvuII CAG'CTG
Cuts at: 1726 2086 1726
Size: 360 3941
RsrII CG'GwC_CG
Cuts at: 2496 2496
Size: 4301
SacI G_AGCT'C
Cuts at: 1315 1315
Size: 4301
SalI G'TCGA_C
Cuts at: 1338 1338
Size: 4301
SapI GCTCTTCn'nnn_
Cuts at: 753 2324 2534 753
Size: 1571 210 2520
Fragments arranged by size:
2520 1571 210
SbfI CC_TGCA'GG
Cuts at: 1348 1348
Size: 4301
ScaI AGT'ACT
Cuts at: 3818 3818
Size: 4301
SfcI C'TryA_G
Cuts at: 33 509 652 1014 1344 2029 3022 3213 33
Size: 476 143 362 330 685 993 191 1121
Fragments arranged by size:
1121 993 685 476 362 330 191 143
SfoI GGC'GCC
Cuts at: 1733 1981 1733
Size: 248 4053
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
SmaI CCC'GGG
Cuts at: 1323 1323
Size: 4301
SmlI C'TyrA_G
Cuts at: 325 1096 1456 2371 2863 3125 325
Size: 771 360 915 492 262 1501
Fragments arranged by size:
1501 915 771 492 360 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4301
SphI G_CATG'C
Cuts at: 1354 1854 1354
Size: 500 3801
SspI AAT'ATT
Cuts at: 674 674
Size: 4301
StyI C'CwwG_G
Cuts at: 1435 1789 1435
Size: 354 3947
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1884 2017 2476 2685 1884
Size: 133 459 209 3500
Fragments arranged by size:
3500 459 209 133
TatI w'GTAC_w
Cuts at: 312 420 609 3816 312
Size: 108 189 3207 797
Fragments arranged by size:
3207 797 189 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1266 1298 3818 3850 1266
Size: 32 2520 32 1717
Fragments arranged by size:
2520 1717 32 32
Tth111I GACn'n_nGTC
Cuts at: 2098 2098
Size: 4301
XbaI T'CTAG_A
Cuts at: 1332 1332
Size: 4301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 576 1796 576
Size: 1220 3081
XmaI C'CCGG_G
Cuts at: 1321 1321
Size: 4301
XmnI GAAnn'nnTTC
Cuts at: 1211 1211
Size: 4301
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI ApaI AscI AvrII
BbsI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsgI
BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI