Restriction map for
pF1KB7956
(Circular) (Six-base) MAPSORT of: pF1KB7956.seq Check: 5838 from: 1 to: 4298
pF1KB7956 4298 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 737 737
Size: 4298
AccI GT'mk_AC
Cuts at: 1062 1336 1062
Size: 274 4024
Acc65I G'GTAC_C
Cuts at: 1314 1314
Size: 4298
AclI AA'CG_TT
Cuts at: 4138 4138
Size: 4298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 1029 2123 2555 3302 3570 323
Size: 706 1094 432 747 268 1051
Fragments arranged by size:
1094 1051 747 706 432 268
AflIII A'CryG_T
Cuts at: 137 1650 2754 3731 137
Size: 1513 1104 977 704
Fragments arranged by size:
1513 1104 977 704
AgeI A'CCGG_T
Cuts at: 2686 2686
Size: 4298
AlwNI CAG_nnn'CTG
Cuts at: 246 903 1072 1603 3170 246
Size: 657 169 531 1567 1374
Fragments arranged by size:
1567 1374 657 531 169
ApaI G_GGCC'C
Cuts at: 471 471
Size: 4298
ApaLI G'TGCA_C
Cuts at: 3068 3068
Size: 4298
ApoI r'AATT_y
Cuts at: 1302 3651 3907 1302
Size: 2349 256 1693
Fragments arranged by size:
2349 1693 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4298
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
AvaI C'yCGr_G
Cuts at: 1318 1318
Size: 4298
BamHI G'GATC_C
Cuts at: 175 1323 175
Size: 1148 3150
BanI G'GyrC_C
Cuts at: 552 619 1314 1728 1976 2011 552
Size: 67 695 414 248 35 2839
Fragments arranged by size:
2839 695 414 248 67 35
BanII G_rGCy'C
Cuts at: 152 471 541 1312 2342 152
Size: 319 70 771 1030 2108
Fragments arranged by size:
2108 1030 771 319 70
BbeI G_GCGC'C
Cuts at: 1732 1980 1732
Size: 248 4050
BciVI GTATCCnnnnn_n'
Cuts at: 2196 2957 4298 2196
Size: 761 1341 2196
Fragments arranged by size:
2196 1341 761
BclI T'GATC_A
Cuts at: 1818 1818
Size: 4298
BglI GCCn_nnn'nGGC
Cuts at: 421 421
Size: 4298
BglII A'GATC_T
Cuts at: 1813 3850 1813
Size: 2037 2261
BlpI GC'TnA_GC
Cuts at: 932 1410 932
Size: 478 3820
Bme1580I G_kGCm'C
Cuts at: 471 638 1923 2016 3072 471
Size: 167 1285 93 1056 1697
Fragments arranged by size:
1697 1285 1056 167 93
BmrI ACTGGGnnnn_n'
Cuts at: 503 625 1247 1510 1591 1926 4070 503
Size: 122 622 263 81 335 2144 731
Fragments arranged by size:
2144 731 622 335 263 122 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 4298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 194 205 493 862 925 1168 1208 166
Size: 28 11 288 369 63 243 40 3256
Fragments arranged by size:
3256 369 288 243 63 40 28 11
Bpu10I CC'TnA_GC
Cuts at: 800 800
Size: 4298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 1246 1474 2353 2845 3143 745
Size: 501 228 879 492 298 1900
Fragments arranged by size:
1900 879 501 492 298 228
BsaI GGTCTCn'nnnn_
Cuts at: 1144 4002 1144
Size: 2858 1440
BsaAI yAC'GTr
Cuts at: 1651 1651
Size: 4298
BsaBI GATnn'nnATC
Cuts at: 6 1812 3849 6
Size: 1806 2037 455
Fragments arranged by size:
2037 1806 455
BsaHI Gr'CG_yC
Cuts at: 1729 1977 2679 4175 1729
Size: 248 702 1496 1852
Fragments arranged by size:
1852 1496 702 248
BsaWI w'CCGG_w
Cuts at: 219 262 1265 1487 2008 2686 2960 3107 3518
Size: 43 1003 222 521 678 274 147 411
Cuts at: 3518 219
Size: 999
Fragments arranged by size:
1003 999 678 521 411 274 222 147
43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 417 447 904 934 417
Size: 30 457 30 3781
Fragments arranged by size:
3781 457 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 697 763 697
Size: 66 4232
BseYI C'CCAG_C
Cuts at: 189 540 696 1039 1072 1723 2481 3058 3554
Size: 351 156 343 33 651 758 577 496
Cuts at: 3554 189
Size: 933
Fragments arranged by size:
933 758 651 577 496 351 343 156
33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 851
Size: 4298
BsiEI CG_ry'CG
Cuts at: 104 1840 1886 3094 104
Size: 1736 46 1208 1308
Fragments arranged by size:
1736 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1312 2090 2280 3072 1312
Size: 778 190 792 2538
Fragments arranged by size:
2538 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 152 471 541 638 1312 1923 2016 2090 2280
Size: 319 70 97 674 611 93 74 190
Cuts at: 2280 2342 3072 152
Size: 62 730 1378
Fragments arranged by size:
1378 730 674 611 319 190 97 93
74 70 62
BspEI T'CCGG_A
Cuts at: 3518 3518
Size: 4298
BspHI T'CATG_A
Cuts at: 3474 3474
Size: 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 137 737 1348 1562 1864 2245 137
Size: 600 611 214 302 381 2190
Fragments arranged by size:
2190 611 600 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2590 2644 4128 4293 50
Size: 2540 54 1484 165 55
Fragments arranged by size:
2540 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2210 2210
Size: 4298
BsrFI r'CCGG_y
Cuts at: 2296 2477 2686 2296
Size: 181 209 3908
Fragments arranged by size:
3908 209 181
BsrGI T'GTAC_A
Cuts at: 640 640
Size: 4298
BssSI C'ACGA_G
Cuts at: 2569 2927 2569
Size: 358 3940
BstAPI GCAn_nnn'nTGC
Cuts at: 1675 1675
Size: 4298
BstBI TT'CG_AA
Cuts at: 2659 2659
Size: 4298
BstYI r'GATC_y
Cuts at: 1 111 175 1323 1790 1813 2148 2394 3406
Size: 110 64 1148 467 23 335 246 1012
Cuts at: 3406 3492 3504 3850 1
Size: 86 12 346 449
Fragments arranged by size:
1148 1012 467 449 346 335 246 110
86 64 23 12
BtgI C'CryG_G
Cuts at: 107 273 107
Size: 166 4132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2248 2429 2592 2248
Size: 181 163 3954
Fragments arranged by size:
3954 181 163
BtsI GCAGTG_nn'
Cuts at: 198 372 1219 1541 198
Size: 174 847 322 2955
Fragments arranged by size:
2955 847 322 174
DraI TTT'AAA
Cuts at: 1297 1297
Size: 4298
DraIII CAC_nnn'GTG
Cuts at: 1101 1101
Size: 4298
DrdI GACnn_nn'nnGTC
Cuts at: 2004 2862 2004
Size: 858 3440
EaeI y'GGCC_r
Cuts at: 1883 2057 2448 2475 3820 1883
Size: 174 391 27 1345 2361
Fragments arranged by size:
2361 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1883 1883
Size: 4298
EarI CTCTTCn'nnn_
Cuts at: 2321 2531 2321
Size: 210 4088
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 831 928 2506 2816 2962 4108 831
Size: 97 1578 310 146 1146 1021
Fragments arranged by size:
1578 1146 1021 310 146 97
EcoICRI GAG'CTC
Cuts at: 1310 1310
Size: 4298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 194 205 323 493 862 925 1029 1168
Size: 28 11 118 170 369 63 104 139
Cuts at: 1168 1208 2123 2555 3302 3570 166
Size: 40 915 432 747 268 894
Fragments arranged by size:
915 894 747 432 369 268 170 139
118 104 63 40 28 11
EcoNI CCTnn'n_nnAGG
Cuts at: 739 763 739
Size: 24 4274
EcoO109I rG'GnC_Cy
Cuts at: 467 468 756 1437 467
Size: 1 288 681 3328
Fragments arranged by size:
3328 681 288 1
EcoRI G'AATT_C
Cuts at: 1302 1302
Size: 4298
EcoRV GAT'ATC
Cuts at: 162 162
Size: 4298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 4298
FspI TGC'GCA
Cuts at: 2079 2079
Size: 4298
HaeII r_GCGC'y
Cuts at: 1732 1980 3002 3981 1732
Size: 248 1022 979 2049
Fragments arranged by size:
2049 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 2140 2170 2172 2202 151
Size: 32 1957 30 2 30 2247
Fragments arranged by size:
2247 1957 32 30 30 2
HincII GTy'rAC
Cuts at: 1063 1337 1063
Size: 274 4024
HindIII A'AGCT_T
Cuts at: 316 316
Size: 4298
KasI G'GCGC_C
Cuts at: 1728 1976 1728
Size: 248 4050
KpnI G_GTAC'C
Cuts at: 1318 1318
Size: 4298
MluI A'CGCG_T
Cuts at: 137 3731 137
Size: 3594 704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 995 2969 3153 995
Size: 1974 184 2140
Fragments arranged by size:
2140 1974 184
MscI TGG'CCA
Cuts at: 2059 2059
Size: 4298
MslI CAynn'nnrTG
Cuts at: 1283 2287 2414 1283
Size: 1004 127 3167
Fragments arranged by size:
3167 1004 127
MspA1I CmG'CkG
Cuts at: 945 1409 1723 2083 3096 3341 945
Size: 464 314 360 1013 245 1902
Fragments arranged by size:
1902 1013 464 360 314 245
NaeI GCC'GGC
Cuts at: 2479 2479
Size: 4298
NarI GG'CG_CC
Cuts at: 1729 1977 1729
Size: 248 4050
NcoI C'CATG_G
Cuts at: 107 107
Size: 4298
NgoMIV G'CCGG_C
Cuts at: 2477 2477
Size: 4298
NspI r_CATG'y
Cuts at: 1282 1351 1851 2758 1282
Size: 69 500 907 2822
Fragments arranged by size:
2822 907 500 69
PasI CC'CwG_GG
Cuts at: 419 419
Size: 4298
PciI A'CATG_T
Cuts at: 2754 2754
Size: 4298
PflMI CCAn_nnn'nTGG
Cuts at: 903 1072 903
Size: 169 4129
PfoI T'CCnGG_A
Cuts at: 182 896 182
Size: 714 3584
PmeI GTTT'AAAC
Cuts at: 1297 1297
Size: 4298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 4266
PpuMI rG'GwC_Cy
Cuts at: 756 756
Size: 4298
PspOMI G'GGCC_C
Cuts at: 467 467
Size: 4298
PstI C_TGCA'G
Cuts at: 950 1345 2030 950
Size: 395 685 3218
Fragments arranged by size:
3218 685 395
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4298
PvuII CAG'CTG
Cuts at: 945 1723 2083 945
Size: 778 360 3160
Fragments arranged by size:
3160 778 360
RsrII CG'GwC_CG
Cuts at: 1268 2493 1268
Size: 1225 3073
SacI G_AGCT'C
Cuts at: 1312 1312
Size: 4298
SalI G'TCGA_C
Cuts at: 1061 1335 1061
Size: 274 4024
SapI GCTCTTCn'nnn_
Cuts at: 2321 2531 2321
Size: 210 4088
SbfI CC_TGCA'GG
Cuts at: 1345 1345
Size: 4298
ScaI AGT'ACT
Cuts at: 3815 3815
Size: 4298
SfcI C'TryA_G
Cuts at: 33 232 946 1341 2026 3019 3210 33
Size: 199 714 395 685 993 191 1121
Fragments arranged by size:
1121 993 714 685 395 199 191
SfoI GGC'GCC
Cuts at: 1730 1978 1730
Size: 248 4050
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
SmaI CCC'GGG
Cuts at: 1320 1320
Size: 4298
SmlI C'TyrA_G
Cuts at: 760 1225 1453 2368 2860 3122 760
Size: 465 228 915 492 262 1936
Fragments arranged by size:
1936 915 492 465 262 228
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4298
SphI G_CATG'C
Cuts at: 1282 1351 1851 1282
Size: 69 500 3729
Fragments arranged by size:
3729 500 69
StuI AGG'CCT
Cuts at: 509 509
Size: 4298
StyI C'CwwG_G
Cuts at: 107 740 923 1193 1432 1786 107
Size: 633 183 270 239 354 2619
Fragments arranged by size:
2619 633 354 270 239 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 691 1881 2014 2473 2682 691
Size: 1190 133 459 209 2307
Fragments arranged by size:
2307 1190 459 209 133
TatI w'GTAC_w
Cuts at: 341 640 827 3813 341
Size: 299 187 2986 826
Fragments arranged by size:
2986 826 299 187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 249 281 904 936 3815 3847 249
Size: 32 623 32 2879 32 700
Fragments arranged by size:
2879 700 623 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2095 2095
Size: 4298
XbaI T'CTAG_A
Cuts at: 1329 1329
Size: 4298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1793 1793
Size: 4298
XmaI C'CCGG_G
Cuts at: 1318 1318
Size: 4298
XmnI GAAnn'nnTTC
Cuts at: 319 319
Size: 4298
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI