Restriction map for pF1KB6956
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BciVI       BclI        BcnI        BfrI        
BglI        BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaAI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeIII      HgaI        HincII      HindII      HindIII     
HinfI       HpaI        KasI        KpnI        KspI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MunI        NarI        NciI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        TspRI       
Tth111I     Van91I      XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AatII         290  GACGTC               GACGT^C
AccII           5  CGCG                 CG^CG
AciI           15  GCGG                 CCGC(-3/-1)
AcsI          157  GAATTC               [GA]^AATT[CT]
AcyI          290  GACGTC               G[GA]^CG[CT]C
AluI          519  AGCT                 AG^CT
ApoI          157  GAATTC               [GA]^AATT[CT]
Asp700I       659  GAAGTCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          224  CCCGAG               C^[CT]CG[GA]G
AvaII         103  GGTCC                G^G[AT]CC
BcgI          245  CGACAGGCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI          137  CTCCAG               CTGGAG(16/14)
BsaHI         290  GACGTC               G[GA]^CG[CT]C
BsoBI         224  CCCGAG               C^[CT]CG[GA]G
BsrFI         368  GCCGGC               [GA]^CCGG[CT]
BstAPI        178  GCAGAAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         160  TTCGAA               TT^CGAA
BstEII         46  GGTAACC              G^GT[ACGT]ACC
BstF5I        201  GGATG                GGATG(2/0)
BstPI          46  GGTAACC              G^GT[ACGT]ACC
BstUI           5  CGCG                 CG^CG
CfoI           62  GCGC                 GCG^C
Cfr10I        368  GCCGGC               [GA]^CCGG[CT]
Cfr13I        103  GGTCC                G^G[ACGT]CC
DdeI          526  CTCAG                C^T[ACGT]AG
EarI          500  CTCTTC               CTCTTC(1/4)
EcoO65I        46  GGTAACC              G^GT[ACGT]ACC
EcoRI         157  GAATTC               G^AATTC
EcoT14I        32  CCAAGG               C^C[AT][AT]GG
FokI          201  GGATG                GGATG(9/13)
HaeII          61  GGCGCT               [GA]GCGC^[CT]
HapII         369  CCGG                 C^CGG
HhaI           62  GCGC                 GCG^C
Hin1I         290  GACGTC               G[GA]^CG[CT]C
HinP1I         62  GCGC                 G^CGC
HpaII         369  CCGG                 C^CGG
HphI          210  GGTGA                GGTGA(8/7)
Ksp632I       500  CTCTTC               CTCTTC(1/4)
MseI          109  TTAA                 T^TAA
MspI          369  CCGG                 C^CGG
MvnI            5  CGCG                 CG^CG
NaeI          368  GCCGGC               GCC^GGC
NgoMIV        368  GCCGGC               G^CCGGC
NlaIII        252  CATG                 CATG^
NlaIV         220  GGCTCC               GG[ACGT]^[ACGT]CC
NspI          251  GCATGC               [GA]CATG^[CT]
NspV          160  TTCGAA               TT^CGAA
PshAI         455  GACGAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau96I        103  GGTCC                G^G[ACGT]CC
SfuI          160  TTCGAA               TT^CGAA
SphI          251  GCATGC               GCATG^C
StyI           32  CCAAGG               C^C[AT][AT]GG
Tru9I         109  TTAA                 T^TAA
Tsp45I        387  GTCAC                ^GT[GC]AC
Tsp509I       158  AATT                 ^AATT
XbaI          394  TCTAGA               T^CTAGA
XmnI          659  GAAGTCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AfaI           18  GTAC                 GT^AC
              190  GTAC                
BbvI          440  GCAGC                GCAGC(8/12)
              623  GCAGC               
BseRI         476  CTCCTC               GAGGAG(10/8)
               23  GAGGAG              
BspMI         282  ACCTGC               ACCTGC(4/8)
              383  GCAGGT              
BsrI           58  ACTGG                ACTGG(1/-1)
              198  ACTGG               
Fnu4HI        440  GCAGC                GC^[ACGT]GC
              623  GCAGC               
ItaI          440  GCAGC                GC^[ACGT]GC
              623  GCAGC               
MaeII         267  ACGT                 A^CGT
              291  ACGT                
RsaI           18  GTAC                 GT^AC
              190  GTAC                
TseI          440  GCAGC                G^C[AT]GC
              623  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AlwI        BfaI        BglII       BsaBI       BsaJI       
BsiYI       BslI        BsmFI       BssKI       BstNI       
BstYI       Cac8I       DpnII       EcoRII      MaeI        
MaeIII      MamI        MboI        MboII       MflI        
MnlI        MvaI        MwoI        NdeII       Sau3AI      
ScrFI       SfaNI       TaqI        TthHB8I     XhoII       
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