Restriction map for pF1KB6956
 (Circular) (Six-base) MAPSORT of: pF1KB6956.seq  Check: 8750  from: 1  to: 3827



pF1KB6956  3827 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    399     399 
   Size:       3827
 
AatII G_ACGT'C

Cuts at:    402     402 
   Size:       3827
 
AccI GT'mk_AC

Cuts at:    865     865 
   Size:       3827
 
Acc65I G'GTAC_C

Cuts at:    843     843 
   Size:       3827
 
AclI AA'CG_TT

Cuts at:   3667    3667 
   Size:       3827
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1652    2084    2831    3099    1652 
   Size:        432     747     268    2380

  Fragments arranged by size: 

                2380     747     432     268
 
AflIII A'CryG_T

Cuts at:   1179    2283    3260    1179 
   Size:       1104     977    1746

  Fragments arranged by size: 

                1746    1104     977
 
AgeI A'CCGG_T

Cuts at:   2215    2215 
   Size:       3827
 
AlwNI CAG_nnn'CTG

Cuts at:   1132    2699    1132 
   Size:       1567    2260
 
ApaLI G'TGCA_C

Cuts at:   2597    2597 
   Size:       3827
 
ApoI r'AATT_y

Cuts at:    265     831    3180    3436     265 
   Size:        566    2349     256     656

  Fragments arranged by size: 

                2349     656     566     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3827
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3827
 
AvaI C'yCGr_G

Cuts at:    332     847     332 
   Size:        515    3312
 
BamHI G'GATC_C

Cuts at:    852     852 
   Size:       3827
 
BanI G'GyrC_C

Cuts at:    843    1257    1505    1540     843 
   Size:        414     248      35    3130

  Fragments arranged by size: 

                3130     414     248      35
 
BanII G_rGCy'C

Cuts at:    841    1871     841 
   Size:       1030    2797
 
BbeI G_GCGC'C

Cuts at:   1261    1509    1261 
   Size:        248    3579
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    342     376     342 
   Size:         34    3793
 
BciVI GTATCCnnnnn_n'

Cuts at:   1725    2486    3827    1725 
   Size:        761    1341    1725

  Fragments arranged by size: 

                1725    1341     761
 
BclI T'GATC_A

Cuts at:   1347    1347 
   Size:       3827
 
BglII A'GATC_T

Cuts at:    598     640     748    1342    3379     598 
   Size:         42     108     594    2037    1046

  Fragments arranged by size: 

                2037    1046     594     108      42
 
BlpI GC'TnA_GC

Cuts at:    939     939 
   Size:       3827
 
Bme1580I G_kGCm'C

Cuts at:   1452    1545    2601    1452 
   Size:         93    1056    2678

  Fragments arranged by size: 

                2678    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1039    1120    1455    3599    1039 
   Size:         81     335    2144    1267

  Fragments arranged by size: 

                2144    1267     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    260    2194     260 
   Size:       1934    1893
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    230     230 
   Size:       3827
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1003    1882    2374    2672    1003 
   Size:        879     492     298    2158

  Fragments arranged by size: 

                2158     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3531    3531 
   Size:       3827
 
BsaAI yAC'GTr

Cuts at:   1180    1180 
   Size:       3827
 
BsaBI GATnn'nnATC

Cuts at:      6     464     597     621     702     753    1341    3378       6 
   Size:        458     133      24      81      51     588    2037     455

  Fragments arranged by size: 

                2037     588     458     455     133      81      51      24
            
 
BsaHI Gr'CG_yC

Cuts at:    399    1258    1506    2208    3704     399 
   Size:        859     248     702    1496     522

  Fragments arranged by size: 

                1496     859     702     522     248
 
BsaWI w'CCGG_w

Cuts at:   1016    1537    2215    2489    2636    3047    1016 
   Size:        521     678     274     147     411    1796

  Fragments arranged by size: 

                1796     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    360     390     360 
   Size:         30    3797
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    146     575     146 
   Size:        429    3398
 
BseYI C'CCAG_C

Cuts at:   1252    2010    2587    3083    1252 
   Size:        758     577     496    1996

  Fragments arranged by size: 

                1996     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1369    1415    2623     104 
   Size:       1265      46    1208    1308

  Fragments arranged by size: 

                1308    1265    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    841    1619    1809    2601     841 
   Size:        778     190     792    2067

  Fragments arranged by size: 

                2067     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    841    1452    1545    1619    1809    1871    2601     841 
   Size:        611      93      74     190      62     730    2067

  Fragments arranged by size: 

                2067     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3047    3047 
   Size:       3827
 
BspHI T'CATG_A

Cuts at:   3003    3003 
   Size:       3827
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    399     482     877    1091    1393    1774     399 
   Size:         83     395     214     302     381    2452

  Fragments arranged by size: 

                2452     395     381     302     214      83
 
BsrBI CCG'CTC

Cuts at:     50    2119    2173    3657    3822      50 
   Size:       2069      54    1484     165      55

  Fragments arranged by size: 

                2069    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1739    1739 
   Size:       3827
 
BsrFI r'CCGG_y

Cuts at:    476    1825    2006    2215     476 
   Size:       1349     181     209    2088

  Fragments arranged by size: 

                2088    1349     209     181
 
BssSI C'ACGA_G

Cuts at:   2098    2456    2098 
   Size:        358    3469
 
BstAPI GCAn_nnn'nTGC

Cuts at:    292    1204     292 
   Size:        912    2915
 
BstBI TT'CG_AA

Cuts at:    269    2188     269 
   Size:       1919    1908
 
BstEII G'GTnAC_C

Cuts at:    154     154 
   Size:       3827
 
BstYI r'GATC_y

Cuts at:      1     273     598     640     703     748     852    1319    1342 
   Size:        272     325      42      63      45     104     467      23

Cuts at:   1342    1677    1923    2935    3021    3033    3379       1 
   Size:        335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     325     272     246
                 104      86      63      45      42      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1777    1958    2121    1777 
   Size:        181     163    3483

  Fragments arranged by size: 

                3483     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1070    1070 
   Size:       3827
 
DraI TTT'AAA

Cuts at:    826     826 
   Size:       3827
 
DrdI GACnn_nn'nnGTC

Cuts at:   1533    2391    1533 
   Size:        858    2969
 
EaeI y'GGCC_r

Cuts at:   1412    1586    1977    2004    3349    1412 
   Size:        174     391      27    1345    1890

  Fragments arranged by size: 

                1890    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1412    1412 
   Size:       3827
 
EarI CTCTTCn'nnn_

Cuts at:    614    1850    2060     614 
   Size:       1236     210    2381

  Fragments arranged by size: 

                2381    1236     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2035    2345    2491    3637    2035 
   Size:        310     146    1146    2225

  Fragments arranged by size: 

                2225    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    839     839 
   Size:       3827
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    230    1652    2084    2831    3099     230 
   Size:       1422     432     747     268     958

  Fragments arranged by size: 

                1422     958     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    966     966 
   Size:       3827
 
EcoRI G'AATT_C

Cuts at:    265     831     265 
   Size:        566    3261
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    644    1846     644 
   Size:       1202    2625
 
FspI TGC'GCA

Cuts at:   1608    1608 
   Size:       3827
 
HaeII r_GCGC'y

Cuts at:    173    1261    1509    2531    3510     173 
   Size:       1088     248    1022     979     490

  Fragments arranged by size: 

                1088    1022     979     490     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1669    1699    1701    1731    1669 
   Size:         30       2      30    3765

  Fragments arranged by size: 

                3765      30      30       2
 
HincII GTy'rAC

Cuts at:    866     866 
   Size:       3827
 
KasI G'GCGC_C

Cuts at:   1257    1505    1257 
   Size:        248    3579
 
KpnI G_GTAC'C

Cuts at:    847     847 
   Size:       3827
 
MluI A'CGCG_T

Cuts at:   3260    3260 
   Size:       3827
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2498    2682    2498 
   Size:        184    3643
 
MscI TGG'CCA

Cuts at:   1588    1588 
   Size:       3827
 
MslI CAynn'nnrTG

Cuts at:   1816    1943    1816 
   Size:        127    3700
 
MspA1I CmG'CkG

Cuts at:    938    1252    1612    2625    2870     938 
   Size:        314     360    1013     245    1895

  Fragments arranged by size: 

                1895    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:    478    2008     478 
   Size:       1530    2297
 
NarI GG'CG_CC

Cuts at:   1258    1506    1258 
   Size:        248    3579
 
NgoMIV G'CCGG_C

Cuts at:    476    2006     476 
   Size:       1530    2297
 
NspI r_CATG'y

Cuts at:    363     880    1380    2287     363 
   Size:        517     500     907    1903

  Fragments arranged by size: 

                1903     907     517     500
 
PciI A'CATG_T

Cuts at:   2283    2283 
   Size:       3827
 
PfoI T'CCnGG_A

Cuts at:    246     246 
   Size:       3827
 
PmeI GTTT'AAAC

Cuts at:    826     826 
   Size:       3827
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2966    2998    2966 
   Size:         32    3795
 
PshAI GACnn'nnGTC

Cuts at:    567     567 
   Size:       3827
 
PstI C_TGCA'G

Cuts at:    874    1559     874 
   Size:        685    3142
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3827
 
PvuII CAG'CTG

Cuts at:   1252    1612    1252 
   Size:        360    3467
 
RsrII CG'GwC_CG

Cuts at:   2022    2022 
   Size:       3827
 
SacI G_AGCT'C

Cuts at:    841     841 
   Size:       3827
 
SalI G'TCGA_C

Cuts at:    864     864 
   Size:       3827
 
SapI GCTCTTCn'nnn_

Cuts at:   1850    2060    1850 
   Size:        210    3617
 
SbfI CC_TGCA'GG

Cuts at:    874     874 
   Size:       3827
 
ScaI AGT'ACT

Cuts at:   3344    3344 
   Size:       3827
 
SfcI C'TryA_G

Cuts at:     33     870    1555    2548    2739      33 
   Size:        837     685     993     191    1121

  Fragments arranged by size: 

                1121     993     837     685     191
 
SfoI GGC'GCC

Cuts at:   1259    1507    1259 
   Size:        248    3579
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3827
 
SmaI CCC'GGG

Cuts at:    849     849 
   Size:       3827
 
SmlI C'TyrA_G

Cuts at:    982    1897    2389    2651     982 
   Size:        915     492     262    2158

  Fragments arranged by size: 

                2158     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3827
 
SphI G_CATG'C

Cuts at:    363     880    1380     363 
   Size:        517     500    2810

  Fragments arranged by size: 

                2810     517     500
 
StyI C'CwwG_G

Cuts at:    140     961    1315     140 
   Size:        821     354    2652

  Fragments arranged by size: 

                2652     821     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1410    1543    2002    2211    1410 
   Size:        133     459     209    3026

  Fragments arranged by size: 

                3026     459     209     133
 
TatI w'GTAC_w

Cuts at:   3342    3342 
   Size:       3827
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3344    3376    3344 
   Size:         32    3795
 
Tth111I GACn'n_nGTC

Cuts at:   1624    1624 
   Size:       3827
 
XbaI T'CTAG_A

Cuts at:    502     858     502 
   Size:        356    3471
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1322    1322 
   Size:       3827
 
XmaI C'CCGG_G

Cuts at:    847     847 
   Size:       3827
 
XmnI GAAnn'nnTTC

Cuts at:    771     771 
   Size:       3827
 
ZraI GAC'GTC

Cuts at:    400     400 
   Size:       3827
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
   BstEII    BstYI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI     PfoI
     PmeI     PpiI    PshAI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII    BstXI
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PmlI    PpuMI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
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