Restriction map for pF1KB6274
 (Circular) (Six-base) MAPSORT of: pF1KB6274.seq  Check: 783  from: 1  to: 5204



pF1KB6274  5204 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AccI GT'mk_AC

Cuts at:   2242    2242 
   Size:       5204
 
Acc65I G'GTAC_C

Cuts at:   2220    2220 
   Size:       5204
 
AclI AA'CG_TT

Cuts at:   5044    5044 
   Size:       5204
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    721     820    1143    1570    3029    3461    4208    4476     721 
   Size:         99     323     427    1459     432     747     268    1449

  Fragments arranged by size: 

                1459    1449     747     432     427     323     268      99
            
 
AfeI AGC'GCT

Cuts at:   1114    2066    1114 
   Size:        952    4252
 
AflIII A'CryG_T

Cuts at:   1308    2556    3660    4637    1308 
   Size:       1248    1104     977    1875

  Fragments arranged by size: 

                1875    1248    1104     977
 
AgeI A'CCGG_T

Cuts at:   3592    3592 
   Size:       5204
 
AleI CACnn'nnGTG

Cuts at:   1297    2131    1297 
   Size:        834    4370
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2174    2174 
   Size:       5204
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1913    1945    1913 
   Size:         32    5172
 
AlwNI CAG_nnn'CTG

Cuts at:    260     297     370    1912    2185    2509    4076     260 
   Size:         37      73    1542     273     324    1567    1388

  Fragments arranged by size: 

                1567    1542    1388     324     273      73      37
 
ApaI G_GGCC'C

Cuts at:    124     382    1063     124 
   Size:        258     681    4265

  Fragments arranged by size: 

                4265     681     258
 
ApaLI G'TGCA_C

Cuts at:    481     520    1934    2149    3974     481 
   Size:         39    1414     215    1825    1711

  Fragments arranged by size: 

                1825    1711    1414     215      39
 
ApoI r'AATT_y

Cuts at:    343    2208    4557    4813     343 
   Size:       1865    2349     256     734

  Fragments arranged by size: 

                2349    1865     734     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5204
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5204
 
AvaI C'yCGr_G

Cuts at:   1630    1844    2224    1630 
   Size:        214     380    4610

  Fragments arranged by size: 

                4610     380     214
 
BamHI G'GATC_C

Cuts at:   2229    2229 
   Size:       5204
 
BanI G'GyrC_C

Cuts at:    584     912    2058    2220    2634    2882    2917     584 
   Size:        328    1146     162     414     248      35    2871

  Fragments arranged by size: 

                2871    1146     414     328     248     162      35
 
BanII G_rGCy'C

Cuts at:    124     180     382     609    1063    1225    1235    1472    1906 
   Size:         56     202     227     454     162      10     237     434

Cuts at:   1906    2218    3248     124 
   Size:        312    1030    2080

  Fragments arranged by size: 

                2080    1030     454     434     312     237     227     202
                 162      56      10
 
BbeI G_GCGC'C

Cuts at:    588     916    2062    2638    2886     588 
   Size:        328    1146     576     248    2906

  Fragments arranged by size: 

                2906    1146     576     328     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    745    1617     745 
   Size:        872    4332
 
BbvCI CC'TCA_GC

Cuts at:   1577    1577 
   Size:       5204
 
BciVI GTATCCnnnnn_n'

Cuts at:   3102    3863    5204    3102 
   Size:        761    1341    3102

  Fragments arranged by size: 

                3102    1341     761
 
BclI T'GATC_A

Cuts at:   2724    2724 
   Size:       5204
 
BglI GCCn_nnn'nGGC

Cuts at:   2015    2024    2015 
   Size:          9    5195
 
BglII A'GATC_T

Cuts at:   1247    2719    4756    1247 
   Size:       1472    2037    1695

  Fragments arranged by size: 

                2037    1695    1472
 
BlpI GC'TnA_GC

Cuts at:    826    2316     826 
   Size:       1490    3714
 
Bme1580I G_kGCm'C

Cuts at:    124     382     485     524     548    1063    1938    2153    2192 
   Size:        258     103      39      24     515     875     215      39

Cuts at:   2192    2829    2922    3978     124 
   Size:        637      93    1056    1350

  Fragments arranged by size: 

                1350    1056     875     637     515     258     215     103
                  93      39      39      24
 
BmgBI CAC'GTC

Cuts at:   1263    1263 
   Size:       5204
 
BmrI ACTGGGnnnn_n'

Cuts at:    855    1971    2173    2416    2497    2832    4976     855 
   Size:       1116     202     243      81     335    2144    1083

  Fragments arranged by size: 

                2144    1116    1083     335     243     202      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3571    3571 
   Size:       5204
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    616     988    2060     616 
   Size:        372    1072    3760

  Fragments arranged by size: 

                3760    1072     372
 
Bpu10I CC'TnA_GC

Cuts at:   1577    1905    1577 
   Size:        328    4876
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    892    2380    3259    3751    4049     892 
   Size:       1488     879     492     298    2047

  Fragments arranged by size: 

                2047    1488     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    682     775    4908     682 
   Size:         93    4133     978

  Fragments arranged by size: 

                4133     978      93
 
BsaAI yAC'GTr

Cuts at:    630    2133    2557     630 
   Size:       1503     424    3277

  Fragments arranged by size: 

                3277    1503     424
 
BsaBI GATnn'nnATC

Cuts at:      6    2718    4755       6 
   Size:       2712    2037     455

  Fragments arranged by size: 

                2712    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    585     893     913    1140    2059    2635    2883    3585    5081 
   Size:        308      20     227     919     576     248     702    1496

Cuts at:   5081     585 
   Size:        708

  Fragments arranged by size: 

                1496     919     708     702     576     308     248     227
                  20
 
BsaWI w'CCGG_w

Cuts at:    851    2393    2914    3592    3866    4013    4424     851 
   Size:       1542     521     678     274     147     411    1631

  Fragments arranged by size: 

                1631    1542     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    445     475     841     871     943     973    1780    1810     445 
   Size:         30     366      30      72      30     807      30    3839

  Fragments arranged by size: 

                3839     807     366      72      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    457     964     991    1801    1804     457 
   Size:        507      27     810       3    3857

  Fragments arranged by size: 

                3857     810     507      27       3
 
BseYI C'CCAG_C

Cuts at:    370    1320    1361    1452    2629    3387    3964    4460     370 
   Size:        950      41      91    1177     758     577     496    1114

  Fragments arranged by size: 

                1177    1114     950     758     577     496      91      41
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    246    1129    1275     246 
   Size:        883     146    4175

  Fragments arranged by size: 

                4175     883     146
 
BsiEI CG_ry'CG

Cuts at:    104    1344    2746    2792    4000     104 
   Size:       1240    1402      46    1208    1308

  Fragments arranged by size: 

                1402    1308    1240    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    485     524    1235    1938    2153    2218    2996    3186    3978 
   Size:         39     711     703     215      65     778     190     792

Cuts at:   3978     485 
   Size:       1711

  Fragments arranged by size: 

                1711     792     778     711     703     215     190      65
                  39
 
BsmBI CGTCTCn'nnnn_

Cuts at:    900     900 
   Size:       5204
 
Bsp1286I G_dGCh'C

Cuts at:    124     180     382     485     524     548     609    1063    1225 
   Size:         56     202     103      39      24      61     454     162

Cuts at:   1225    1235    1472    1906    1938    2153    2192    2218    2829 
   Size:         10     237     434      32     215      39      26     611

Cuts at:   2829    2922    2996    3186    3248    3978     124 
   Size:         93      74     190      62     730    1350

  Fragments arranged by size: 

                1350     730     611     454     434     237     215     202
                 190     162     103      93      74      62      61      56
                  39      39      32      26      24      10
 
BspEI T'CCGG_A

Cuts at:   4424    4424 
   Size:       5204
 
BspHI T'CATG_A

Cuts at:   4380    4380 
   Size:       5204
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2254    2468    2770    3151    2254 
   Size:        214     302     381    4307

  Fragments arranged by size: 

                4307     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     226    1047    1968    3496    3550    5034    5199      50 
   Size:        176     821     921    1528      54    1484     165      55

  Fragments arranged by size: 

                1528    1484     921     821     176     165      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:    307    1685    3116     307 
   Size:       1378    1431    2395

  Fragments arranged by size: 

                2395    1431    1378
 
BsrFI r'CCGG_y

Cuts at:    524     821     972    1343    3202    3383    3592     524 
   Size:        297     151     371    1859     181     209    2136

  Fragments arranged by size: 

                2136    1859     371     297     209     181     151
 
BssSI C'ACGA_G

Cuts at:   3475    3833    3475 
   Size:        358    4846
 
BstAPI GCAn_nnn'nTGC

Cuts at:    260     297     497    1652    2581     260 
   Size:         37     200    1155     929    2883

  Fragments arranged by size: 

                2883    1155     929     200      37
 
BstBI TT'CG_AA

Cuts at:   3565    3565 
   Size:       5204
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1702    1702 
   Size:       5204
 
BstYI r'GATC_y

Cuts at:      1     599     623    1247    1985    2229    2696    2719    3054 
   Size:        598      24     624     738     244     467      23     335

Cuts at:   3054    3300    4312    4398    4410    4756       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     738     624     598     467     449     346     335
                 246     244      86      24      23      12
 
BtgI C'CryG_G

Cuts at:    107     579     917    1853     107 
   Size:        472     338     936    3458

  Fragments arranged by size: 

                3458     936     472     338
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3154    3335    3498    3154 
   Size:        181     163    4860

  Fragments arranged by size: 

                4860     181     163
 
BtsI GCAGTG_nn'

Cuts at:    258    2447     258 
   Size:       2189    3015
 
DraI TTT'AAA

Cuts at:   2203    2203 
   Size:       5204
 
DrdI GACnn_nn'nnGTC

Cuts at:   1267    2086    2910    3768    1267 
   Size:        819     824     858    2703

  Fragments arranged by size: 

                2703     858     824     819
 
EaeI y'GGCC_r

Cuts at:    977    1208    1341    1856    2016    2093    2789    2963    3354 
   Size:        231     133     515     160      77     696     174     391

Cuts at:   3354    3381    4726     977 
   Size:         27    1345    1455

  Fragments arranged by size: 

                1455    1345     696     515     391     231     174     160
                 133      77      27
 
EagI C'GGCC_G

Cuts at:   1341    2789    1341 
   Size:       1448    3756
 
EarI CTCTTCn'nnn_

Cuts at:   1071    1516    3227    3437    1071 
   Size:        445    1711     210    2838

  Fragments arranged by size: 

                2838    1711     445     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    406    3412    3722    3868    5014     406 
   Size:       3006     310     146    1146     596

  Fragments arranged by size: 

                3006    1146     596     310     146
 
EcoICRI GAG'CTC

Cuts at:   1233    2216    1233 
   Size:        983    4221
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    616     721     820     988    1143    1570    2060    3029    3461 
   Size:        105      99     168     155     427     490     969     432

Cuts at:   3461    4208    4476     616 
   Size:        747     268    1344

  Fragments arranged by size: 

                1344     969     747     490     432     427     268     168
                 155     105      99
 
EcoO109I rG'GnC_Cy

Cuts at:    121     148     294     378     379    1060    1333    1545    1658 
   Size:         27     146      84       1     681     273     212     113

Cuts at:   1658    1909    2343     121 
   Size:        251     434    2982

  Fragments arranged by size: 

                2982     681     434     273     251     212     146     113
                  84      27       1
 
EcoRI G'AATT_C

Cuts at:   2208    2208 
   Size:       5204
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1134    3223    1134 
   Size:       2089    3115
 
FseI GG_CCGG'CC

Cuts at:   1347    1347 
   Size:       5204
 
FspI TGC'GCA

Cuts at:   2985    2985 
   Size:       5204
 
HaeII r_GCGC'y

Cuts at:    371     531     579     588     822     916    1116    1321    1899 
   Size:        160      48       9     234      94     200     205     578

Cuts at:   1899    2062    2068    2638    2886    3908    4887     371 
   Size:        163       6     570     248    1022     979     688

  Fragments arranged by size: 

                1022     979     688     578     570     248     234     205
                 200     163     160      94      48       9       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    591     623     841     873    1708    1740    1970    1977    2002 
   Size:         32     218      32     835      32     230       7      25

Cuts at:   2002    2009    3046    3076    3078    3108     591 
   Size:          7    1037      30       2      30    2687

  Fragments arranged by size: 

                2687    1037     835     230     218      32      32      32
                  30      30      25       7       7       2
 
HincII GTy'rAC

Cuts at:   2243    2243 
   Size:       5204
 
KasI G'GCGC_C

Cuts at:    584     912    2058    2634    2882     584 
   Size:        328    1146     576     248    2906

  Fragments arranged by size: 

                2906    1146     576     328     248
 
KpnI G_GTAC'C

Cuts at:   2224    2224 
   Size:       5204
 
MluI A'CGCG_T

Cuts at:   4637    4637 
   Size:       5204
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1178    3875    4059    1178 
   Size:       2697     184    2323

  Fragments arranged by size: 

                2697    2323     184
 
MscI TGG'CCA

Cuts at:   1858    2095    2965    1858 
   Size:        237     870    4097

  Fragments arranged by size: 

                4097     870     237
 
MslI CAynn'nnrTG

Cuts at:   1297    2131    2156    3193    3320    1297 
   Size:        834      25    1037     127    3181

  Fragments arranged by size: 

                3181    1037     834     127      25
 
MspA1I CmG'CkG

Cuts at:    581     594    1452    1952    2024    2315    2629    2989    4002 
   Size:         13     858     500      72     291     314     360    1013

Cuts at:   4002    4247     581 
   Size:        245    1538

  Fragments arranged by size: 

                1538    1013     858     500     360     314     291     245
                  72      13
 
NaeI GCC'GGC

Cuts at:    823    1345    3385     823 
   Size:        522    2040    2642

  Fragments arranged by size: 

                2642    2040     522
 
NarI GG'CG_CC

Cuts at:    585     913    2059    2635    2883     585 
   Size:        328    1146     576     248    2906

  Fragments arranged by size: 

                2906    1146     576     328     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       5204
 
NgoMIV G'CCGG_C

Cuts at:    821    1343    3383     821 
   Size:        522    2040    2642

  Fragments arranged by size: 

                2642    2040     522
 
NspI r_CATG'y

Cuts at:   1611    2257    2757    3664    1611 
   Size:        646     500     907    3151

  Fragments arranged by size: 

                3151     907     646     500
 
PasI CC'CwG_GG

Cuts at:   1888    1888 
   Size:       5204
 
PciI A'CATG_T

Cuts at:   3660    3660 
   Size:       5204
 
PflMI CCAn_nnn'nTGG

Cuts at:    370     370 
   Size:       5204
 
PfoI T'CCnGG_A

Cuts at:   1134    1271    1134 
   Size:        137    5067
 
PmeI GTTT'AAAC

Cuts at:   2203    2203 
   Size:       5204
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4343    4375    4343 
   Size:         32    5172
 
PpuMI rG'GwC_Cy

Cuts at:    148    1333    1545    1658     148 
   Size:       1185     212     113    3694

  Fragments arranged by size: 

                3694    1185     212     113
 
PspOMI G'GGCC_C

Cuts at:    120     378    1059     120 
   Size:        258     681    4265

  Fragments arranged by size: 

                4265     681     258
 
PstI C_TGCA'G

Cuts at:    256     316     593     997    2251    2936     256 
   Size:         60     277     404    1254     685    2524

  Fragments arranged by size: 

                2524    1254     685     404     277      60
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5204
 
PvuII CAG'CTG

Cuts at:    594    1452    2629    2989     594 
   Size:        858    1177     360    2809

  Fragments arranged by size: 

                2809    1177     858     360
 
RsrII CG'GwC_CG

Cuts at:   1389    3399    1389 
   Size:       2010    3194
 
SacI G_AGCT'C

Cuts at:   1235    2218    1235 
   Size:        983    4221
 
SacII CC_GC'GG

Cuts at:    582     582 
   Size:       5204
 
SalI G'TCGA_C

Cuts at:   2241    2241 
   Size:       5204
 
SanDI GG'GwC_CC

Cuts at:    148    1658     148 
   Size:       1510    3694
 
SapI GCTCTTCn'nnn_

Cuts at:   3227    3437    3227 
   Size:        210    4994
 
SbfI CC_TGCA'GG

Cuts at:   2251    2251 
   Size:       5204
 
ScaI AGT'ACT

Cuts at:   4721    4721 
   Size:       5204
 
SfcI C'TryA_G

Cuts at:     33     252     312     429     589     993    1539    2247    2932 
   Size:        219      60     117     160     404     546     708     685

Cuts at:   2932    3925    4116      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                1121     993     708     685     546     404     219     191
                 160     117      60
 
SfoI GGC'GCC

Cuts at:    586     914    2060    2636    2884     586 
   Size:        328    1146     576     248    2906

  Fragments arranged by size: 

                2906    1146     576     328     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5204
 
SgrAI Cr'CCGG_yG

Cuts at:    524     524 
   Size:       5204
 
SmaI CCC'GGG

Cuts at:   1632    1846    2226    1632 
   Size:        214     380    4610

  Fragments arranged by size: 

                4610     380     214
 
SmlI C'TyrA_G

Cuts at:    907    2359    3274    3766    4028     907 
   Size:       1452     915     492     262    2083

  Fragments arranged by size: 

                2083    1452     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5204
 
SphI G_CATG'C

Cuts at:   2257    2757    2257 
   Size:        500    4704
 
StyI C'CwwG_G

Cuts at:    107    2338    2692     107 
   Size:       2231     354    2619

  Fragments arranged by size: 

                2619    2231     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1375    1901    2787    2920    3379    3588    1375 
   Size:        526     886     133     459     209    2991

  Fragments arranged by size: 

                2991     886     526     459     209     133
 
TatI w'GTAC_w

Cuts at:   4719    4719 
   Size:       5204
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    445     477    4721    4753     445 
   Size:         32    4244      32     896

  Fragments arranged by size: 

                4244     896      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3001    3001 
   Size:       5204
 
XbaI T'CTAG_A

Cuts at:   2235    2235 
   Size:       5204
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114     634    2699     114 
   Size:        520    2065    2619

  Fragments arranged by size: 

                2619    2065     520
 
XmaI C'CCGG_G

Cuts at:   1630    1844    2224    1630 
   Size:        214     380    4610

  Fragments arranged by size: 

                4610     380     214
 
XmnI GAAnn'nnTTC

Cuts at:   1624    1624 
   Size:       5204
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FseI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AscI    AvrII     BaeI     BcgI
    BfrBI     BmtI    BsiWI     BsmI    BsrGI   BssHII   BstEII  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PmlI    PshAI     PsiI    PspXI     PsrI    SexAI     SfiI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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