Restriction map for pF1KB6274
 (Linear) (Six-base) MAPSORT of: KIBB6274.seq  Check: 7254  from: 1  to: 2091



KIBB6274.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     613     712    1035    1462    2091 
   Size:        613      99     323     427     629

  Fragments arranged by size: 

                 629     613     427     323      99
 
AfeI AGC'GCT

Cuts at:      0    1006    1958    2091 
   Size:       1006     952     133

  Fragments arranged by size: 

                1006     952     133
 
AflIII A'CryG_T

Cuts at:      0    1200    2091 
   Size:       1200     891
 
AleI CACnn'nnGTG

Cuts at:      0    1189    2023    2091 
   Size:       1189     834      68

  Fragments arranged by size: 

                1189     834      68
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2066    2091 
   Size:       2066      25
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1805    1837    2091 
   Size:       1805      32     254

  Fragments arranged by size: 

                1805     254      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     152     189     262    1804    2077    2091 
   Size:        152      37      73    1542     273      14

  Fragments arranged by size: 

                1542     273     152      73      37      14
 
ApaI G_GGCC'C

Cuts at:      0      16     274     955    2091 
   Size:         16     258     681    1136

  Fragments arranged by size: 

                1136     681     258      16
 
ApaLI G'TGCA_C

Cuts at:      0     373     412    1826    2041    2091 
   Size:        373      39    1414     215      50

  Fragments arranged by size: 

                1414     373     215      50      39
 
ApoI r'AATT_y

Cuts at:      0     235    2091 
   Size:        235    1856
 
AvaI C'yCGr_G

Cuts at:      0    1522    1736    2091 
   Size:       1522     214     355

  Fragments arranged by size: 

                1522     355     214
 
BanI G'GyrC_C

Cuts at:      0     476     804    1950    2091 
   Size:        476     328    1146     141

  Fragments arranged by size: 

                1146     476     328     141
 
BanII G_rGCy'C

Cuts at:      0      16      72     274     501     955    1117    1127    1364 
   Size:         16      56     202     227     454     162      10     237

Cuts at:   1364    1798    2091 
   Size:        434     293

  Fragments arranged by size: 

                 454     434     293     237     227     202     162      56
                  16      10
 
BbeI G_GCGC'C

Cuts at:      0     480     808    1954    2091 
   Size:        480     328    1146     137

  Fragments arranged by size: 

                1146     480     328     137
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     637    1509    2091 
   Size:        637     872     582

  Fragments arranged by size: 

                 872     637     582
 
BbvCI CC'TCA_GC

Cuts at:      0    1469    2091 
   Size:       1469     622
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1907    1916    2091 
   Size:       1907       9     175

  Fragments arranged by size: 

                1907     175       9
 
BglII A'GATC_T

Cuts at:      0    1139    2091 
   Size:       1139     952
 
BlpI GC'TnA_GC

Cuts at:      0     718    2091 
   Size:        718    1373
 
Bme1580I G_kGCm'C

Cuts at:      0      16     274     377     416     440     955    1830    2045 
   Size:         16     258     103      39      24     515     875     215

Cuts at:   2045    2084    2091 
   Size:         39       7

  Fragments arranged by size: 

                 875     515     258     215     103      39      39      24
                  16       7
 
BmgBI CAC'GTC

Cuts at:      0    1155    2091 
   Size:       1155     936
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     747    1863    2065    2091 
   Size:        747    1116     202      26

  Fragments arranged by size: 

                1116     747     202      26
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     880    1952    2091 
   Size:        508     372    1072     139

  Fragments arranged by size: 

                1072     508     372     139
 
Bpu10I CC'TnA_GC

Cuts at:      0    1469    1797    2091 
   Size:       1469     328     294

  Fragments arranged by size: 

                1469     328     294
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     784    2091 
   Size:        784    1307
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     574     667    2091 
   Size:        574      93    1424

  Fragments arranged by size: 

                1424     574      93
 
BsaAI yAC'GTr

Cuts at:      0     522    2025    2091 
   Size:        522    1503      66

  Fragments arranged by size: 

                1503     522      66
 
BsaHI Gr'CG_yC

Cuts at:      0     477     785     805    1032    1951    2091 
   Size:        477     308      20     227     919     140

  Fragments arranged by size: 

                 919     477     308     227     140      20
 
BsaWI w'CCGG_w

Cuts at:      0     743    2091 
   Size:        743    1348
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     337     367     733     763     835     865    1672    1702 
   Size:        337      30     366      30      72      30     807      30

Cuts at:   1702    2091 
   Size:        389

  Fragments arranged by size: 

                 807     389     366     337      72      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     349     856     883    1693    1696    2091 
   Size:        349     507      27     810       3     395

  Fragments arranged by size: 

                 810     507     395     349      27       3
 
BseYI C'CCAG_C

Cuts at:      0     262    1212    1253    1344    2091 
   Size:        262     950      41      91     747

  Fragments arranged by size: 

                 950     747     262      91      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     138    1021    1167    2091 
   Size:        138     883     146     924

  Fragments arranged by size: 

                 924     883     146     138
 
BsiEI CG_ry'CG

Cuts at:      0    1236    2091 
   Size:       1236     855
 
BsiHKAI G_wGCw'C

Cuts at:      0     377     416    1127    1830    2045    2091 
   Size:        377      39     711     703     215      46

  Fragments arranged by size: 

                 711     703     377     215      46      39
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     792    2091 
   Size:        792    1299
 
Bsp1286I G_dGCh'C

Cuts at:      0      16      72     274     377     416     440     501     955 
   Size:         16      56     202     103      39      24      61     454

Cuts at:    955    1117    1127    1364    1798    1830    2045    2084    2091 
   Size:        162      10     237     434      32     215      39       7

  Fragments arranged by size: 

                 454     434     237     215     202     162     103      61
                  56      39      39      32      24      16      10       7
            
 
BsrBI CCG'CTC

Cuts at:      0     118     939    1860    2091 
   Size:        118     821     921     231

  Fragments arranged by size: 

                 921     821     231     118
 
BsrDI GCAATG_nn'

Cuts at:      0     199    1577    2091 
   Size:        199    1378     514

  Fragments arranged by size: 

                1378     514     199
 
BsrFI r'CCGG_y

Cuts at:      0     416     713     864    1235    2091 
   Size:        416     297     151     371     856

  Fragments arranged by size: 

                 856     416     371     297     151
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     152     189     389    1544    2091 
   Size:        152      37     200    1155     547

  Fragments arranged by size: 

                1155     547     200     152      37
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1594    2091 
   Size:       1594     497
 
BstYI r'GATC_y

Cuts at:      0     491     515    1139    1877    2091 
   Size:        491      24     624     738     214

  Fragments arranged by size: 

                 738     624     491     214      24
 
BtgI C'CryG_G

Cuts at:      0     471     809    1745    2091 
   Size:        471     338     936     346

  Fragments arranged by size: 

                 936     471     346     338
 
BtsI GCAGTG_nn'

Cuts at:      0     150    2091 
   Size:        150    1941
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1159    1978    2091 
   Size:       1159     819     113

  Fragments arranged by size: 

                1159     819     113
 
EaeI y'GGCC_r

Cuts at:      0     869    1100    1233    1748    1908    1985    2091 
   Size:        869     231     133     515     160      77     106

  Fragments arranged by size: 

                 869     515     231     160     133     106      77
 
EagI C'GGCC_G

Cuts at:      0    1233    2091 
   Size:       1233     858
 
EarI CTCTTCn'nnn_

Cuts at:      0     963    1408    2091 
   Size:        963     445     683

  Fragments arranged by size: 

                 963     683     445
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     298    2091 
   Size:        298    1793
 
EcoICRI GAG'CTC

Cuts at:      0    1125    2091 
   Size:       1125     966
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     613     712     880    1035    1462    1952    2091 
   Size:        508     105      99     168     155     427     490     139

  Fragments arranged by size: 

                 508     490     427     168     155     139     105      99
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0      13      40     186     270     271     952    1225    1437 
   Size:         13      27     146      84       1     681     273     212

Cuts at:   1437    1550    1801    2091 
   Size:        113     251     290

  Fragments arranged by size: 

                 681     290     273     251     212     146     113      84
                  27      13       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1026    2091 
   Size:       1026    1065
 
FseI GG_CCGG'CC

Cuts at:      0    1239    2091 
   Size:       1239     852
 
HaeII r_GCGC'y

Cuts at:      0     263     423     471     480     714     808    1008    1213 
   Size:        263     160      48       9     234      94     200     205

Cuts at:   1213    1791    1954    1960    2091 
   Size:        578     163       6     131

  Fragments arranged by size: 

                 578     263     234     205     200     163     160     131
                  94      48       9       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     483     515     733     765    1600    1632    1862    1869 
   Size:        483      32     218      32     835      32     230       7

Cuts at:   1869    1894    1901    2091 
   Size:         25       7     190

  Fragments arranged by size: 

                 835     483     230     218     190      32      32      32
                  25       7       7
 
KasI G'GCGC_C

Cuts at:      0     476     804    1950    2091 
   Size:        476     328    1146     141

  Fragments arranged by size: 

                1146     476     328     141
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1070    2091 
   Size:       1070    1021
 
MscI TGG'CCA

Cuts at:      0    1750    1987    2091 
   Size:       1750     237     104

  Fragments arranged by size: 

                1750     237     104
 
MslI CAynn'nnrTG

Cuts at:      0    1189    2023    2048    2091 
   Size:       1189     834      25      43

  Fragments arranged by size: 

                1189     834      43      25
 
MspA1I CmG'CkG

Cuts at:      0     473     486    1344    1844    1916    2091 
   Size:        473      13     858     500      72     175

  Fragments arranged by size: 

                 858     500     473     175      72      13
 
NaeI GCC'GGC

Cuts at:      0     715    1237    2091 
   Size:        715     522     854

  Fragments arranged by size: 

                 854     715     522
 
NarI GG'CG_CC

Cuts at:      0     477     805    1951    2091 
   Size:        477     328    1146     140

  Fragments arranged by size: 

                1146     477     328     140
 
NgoMIV G'CCGG_C

Cuts at:      0     713    1235    2091 
   Size:        713     522     856

  Fragments arranged by size: 

                 856     713     522
 
NspI r_CATG'y

Cuts at:      0    1503    2091 
   Size:       1503     588
 
PasI CC'CwG_GG

Cuts at:      0    1780    2091 
   Size:       1780     311
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     262    2091 
   Size:        262    1829
 
PfoI T'CCnGG_A

Cuts at:      0    1026    1163    2091 
   Size:       1026     137     928

  Fragments arranged by size: 

                1026     928     137
 
PpuMI rG'GwC_Cy

Cuts at:      0      40    1225    1437    1550    2091 
   Size:         40    1185     212     113     541

  Fragments arranged by size: 

                1185     541     212     113      40
 
PspOMI G'GGCC_C

Cuts at:      0      12     270     951    2091 
   Size:         12     258     681    1140

  Fragments arranged by size: 

                1140     681     258      12
 
PstI C_TGCA'G

Cuts at:      0     148     208     485     889    2091 
   Size:        148      60     277     404    1202

  Fragments arranged by size: 

                1202     404     277     148      60
 
PvuII CAG'CTG

Cuts at:      0     486    1344    2091 
   Size:        486     858     747

  Fragments arranged by size: 

                 858     747     486
 
RsrII CG'GwC_CG

Cuts at:      0    1281    2091 
   Size:       1281     810
 
SacI G_AGCT'C

Cuts at:      0    1127    2091 
   Size:       1127     964
 
SacII CC_GC'GG

Cuts at:      0     474    2091 
   Size:        474    1617
 
SanDI GG'GwC_CC

Cuts at:      0      40    1550    2091 
   Size:         40    1510     541

  Fragments arranged by size: 

                1510     541      40
 
SfcI C'TryA_G

Cuts at:      0     144     204     321     481     885    1431    2091 
   Size:        144      60     117     160     404     546     660

  Fragments arranged by size: 

                 660     546     404     160     144     117      60
 
SfoI GGC'GCC

Cuts at:      0     478     806    1952    2091 
   Size:        478     328    1146     139

  Fragments arranged by size: 

                1146     478     328     139
 
SgrAI Cr'CCGG_yG

Cuts at:      0     416    2091 
   Size:        416    1675
 
SmaI CCC'GGG

Cuts at:      0    1524    1738    2091 
   Size:       1524     214     353

  Fragments arranged by size: 

                1524     353     214
 
SmlI C'TyrA_G

Cuts at:      0     799    2091 
   Size:        799    1292
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1267    1793    2091 
   Size:       1267     526     298

  Fragments arranged by size: 

                1267     526     298
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     337     369    2091 
   Size:        337      32    1722

  Fragments arranged by size: 

                1722     337      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     526    2091 
   Size:        526    1565
 
XmaI C'CCGG_G

Cuts at:      0    1522    1736    2091 
   Size:       1522     214     355

  Fragments arranged by size: 

                1522     355     214
 
XmnI GAAnn'nnTTC

Cuts at:      0    1516    2091 
   Size:       1516     575
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BsrBI    BsrDI    BsrFI
   BstAPI    BstXI    BstYI     BtgI     BtsI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI     FseI    HaeII
    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI     PstI
    PvuII    RsrII     SacI    SacII    SanDI     SfcI     SfoI    SgrAI
     SmaI     SmlI    TaqII     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI
     BclI    BfrBI     BmtI     BplI    BsaBI    BsiWI     BsmI    BspEI
    BspHI    BspMI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII    EcoNI    EcoRI
    EcoRV     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI  Tth111I     XbaI     XhoI     ZraI
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