Restriction map for pF1KB6240
 (Linear) (Six-base) MAPSORT of: KIBB6240.seq  Check: 5746  from: 1  to: 2031



KIBB6240.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     507    1112    2031 
   Size:        507     605     919

  Fragments arranged by size: 

                 919     605     507
 
AccI GT'mk_AC

Cuts at:      0    1117    1743    2031 
   Size:       1117     626     288

  Fragments arranged by size: 

                1117     626     288
 
AclI AA'CG_TT

Cuts at:      0    1583    2031 
   Size:       1583     448
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     193     262    1955    2031 
   Size:        169      24      69    1693      76

  Fragments arranged by size: 

                1693     169      76      69      24
 
AflIII A'CryG_T

Cuts at:      0     769    1899    2031 
   Size:        769    1130     132

  Fragments arranged by size: 

                1130     769     132
 
ApaI G_GGCC'C

Cuts at:      0    1943    2031 
   Size:       1943      88
 
ApoI r'AATT_y

Cuts at:      0    1369    1618    2031 
   Size:       1369     249     413

  Fragments arranged by size: 

                1369     413     249
 
AvaI C'yCGr_G

Cuts at:      0     625    2031 
   Size:        625    1406
 
BanII G_rGCy'C

Cuts at:      0     367    1916    1943    2031 
   Size:        367    1549      27      88

  Fragments arranged by size: 

                1549     367      88      27
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      85    1546    2031 
   Size:         85    1461     485

  Fragments arranged by size: 

                1461     485      85
 
BbvCI CC'TCA_GC

Cuts at:      0     368    1139    2031 
   Size:        368     771     892

  Fragments arranged by size: 

                 892     771     368
 
BfrBI ATG'CAT

Cuts at:      0    1411    2031 
   Size:       1411     620
 
BglII A'GATC_T

Cuts at:      0    1469    1870    2031 
   Size:       1469     401     161

  Fragments arranged by size: 

                1469     401     161
 
BlpI GC'TnA_GC

Cuts at:      0     361    2031 
   Size:        361    1670
 
Bme1580I G_kGCm'C

Cuts at:      0    1337    1943    2031 
   Size:       1337     606      88

  Fragments arranged by size: 

                1337     606      88
 
BmgBI CAC'GTC

Cuts at:      0    1902    2031 
   Size:       1902     129
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1226    2031 
   Size:       1226     805
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     361     373    1427    2031 
   Size:        331      30      12    1054     604

  Fragments arranged by size: 

                1054     604     331      30      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     368    1139    2031 
   Size:        368     771     892

  Fragments arranged by size: 

                 892     771     368
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     644    2031 
   Size:        296     348    1387

  Fragments arranged by size: 

                1387     348     296
 
BsaAI yAC'GTr

Cuts at:      0    1523    2031 
   Size:       1523     508
 
BsaBI GATnn'nnATC

Cuts at:      0     243    2031 
   Size:        243    1788
 
BsaHI Gr'CG_yC

Cuts at:      0     121    2031 
   Size:        121    1910
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      91     121    2031 
   Size:         91      30    1910

  Fragments arranged by size: 

                1910      91      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     376     768     771     895    2031 
   Size:        376     392       3     124    1136

  Fragments arranged by size: 

                1136     392     376     124       3
 
BseYI C'CCAG_C

Cuts at:      0     161     326     527    2031 
   Size:        161     165     201    1504

  Fragments arranged by size: 

                1504     201     165     161
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     770    1680    2031 
   Size:        770     910     351

  Fragments arranged by size: 

                 910     770     351
 
BsiEI CG_ry'CG

Cuts at:      0     128    2031 
   Size:        128    1903
 
BsiHKAI G_wGCw'C

Cuts at:      0     714     887    2031 
   Size:        714     173    1144

  Fragments arranged by size: 

                1144     714     173
 
BsmI GAATG_Cn'

Cuts at:      0    1536    2031 
   Size:       1536     495
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     112    2031 
   Size:        112    1919
 
Bsp1286I G_dGCh'C

Cuts at:      0     367     714     887    1337    1916    1943    2031 
   Size:        367     347     173     450     579      27      88

  Fragments arranged by size: 

                 579     450     367     347     173      88      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     507    1026    1112    2031 
   Size:        507     519      86     919

  Fragments arranged by size: 

                 919     519     507      86
 
BsrDI GCAATG_nn'

Cuts at:      0    1832    2031 
   Size:       1832     199
 
BsrFI r'CCGG_y

Cuts at:      0     123    2031 
   Size:        123    1908
 
BsrGI T'GTAC_A

Cuts at:      0    1041    1896    2031 
   Size:       1041     855     135

  Fragments arranged by size: 

                1041     855     135
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     357    1354    1887    2031 
   Size:        357     997     533     144

  Fragments arranged by size: 

                 997     533     357     144
 
BstEII G'GTnAC_C

Cuts at:      0    1651    2031 
   Size:       1651     380
 
BstYI r'GATC_y

Cuts at:      0     341     756    1469    1870    2031 
   Size:        341     415     713     401     161

  Fragments arranged by size: 

                 713     415     401     341     161
 
BstZ17I GTA'TAC

Cuts at:      0    1744    2031 
   Size:       1744     287
 
BtgI C'CryG_G

Cuts at:      0     128    1766    2031 
   Size:        128    1638     265

  Fragments arranged by size: 

                1638     265     128
 
BtsI GCAGTG_nn'

Cuts at:      0     950    1541    2031 
   Size:        950     591     490

  Fragments arranged by size: 

                 950     591     490
 
DraIII CAC_nnn'GTG

Cuts at:      0    1293    2031 
   Size:       1293     738
 
EaeI y'GGCC_r

Cuts at:      0     125     928    1517    2031 
   Size:        125     803     589     514

  Fragments arranged by size: 

                 803     589     514     125
 
EagI C'GGCC_G

Cuts at:      0     125    2031 
   Size:        125    1906
 
EarI CTCTTCn'nnn_

Cuts at:      0     281    1805    1904    2031 
   Size:        281    1524      99     127

  Fragments arranged by size: 

                1524     281     127      99
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     193     262     331     361     373    1427    1955 
   Size:        169      24      69      69      30      12    1054     528

Cuts at:   1955    2031 
   Size:         76

  Fragments arranged by size: 

                1054     528     169      76      69      69      30      24
                  12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     402    1258    1297    1939    1940    2031 
   Size:        402     856      39     642       1      91

  Fragments arranged by size: 

                 856     642     402      91      39       1
 
EcoRI G'AATT_C

Cuts at:      0    1618    2031 
   Size:       1618     413
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     819    2031 
   Size:        819    1212
 
FspI TGC'GCA

Cuts at:      0    1358    2031 
   Size:       1358     673
 
HaeII r_GCGC'y

Cuts at:      0     603    2031 
   Size:        603    1428
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      91     123     294     326     696     728     946     978 
   Size:         91      32     171      32     370      32     218      32

Cuts at:    978    2031 
   Size:       1053

  Fragments arranged by size: 

                1053     370     218     171      91      32      32      32
                  32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     697    1243    1530    2031 
   Size:        134     563     546     287     501

  Fragments arranged by size: 

                 563     546     501     287     134
 
MscI TGG'CCA

Cuts at:      0     930    2031 
   Size:        930    1101
 
MslI CAynn'nnrTG

Cuts at:      0    1106    1282    1489    2031 
   Size:       1106     176     207     542

  Fragments arranged by size: 

                1106     542     207     176
 
MspA1I CmG'CkG

Cuts at:      0     145    2031 
   Size:        145    1886
 
NaeI GCC'GGC

Cuts at:      0     125    2031 
   Size:        125    1906
 
NcoI C'CATG_G

Cuts at:      0    1766    2031 
   Size:       1766     265
 
NdeI CA'TA_TG

Cuts at:      0    1407    2031 
   Size:       1407     624
 
NgoMIV G'CCGG_C

Cuts at:      0     123    2031 
   Size:        123    1908
 
NsiI A_TGCA'T

Cuts at:      0    1413    2031 
   Size:       1413     618
 
NspI r_CATG'y

Cuts at:      0     490     773     823     994    1111    1287    1677    2031 
   Size:        490     283      50     171     117     176     390     354

  Fragments arranged by size: 

                 490     390     354     283     176     171     117      50
            
 
PciI A'CATG_T

Cuts at:      0     769    2031 
   Size:        769    1262
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     534    2031 
   Size:        534    1497
 
PmlI CAC'GTG

Cuts at:      0    1523    2031 
   Size:       1523     508
 
PpuMI rG'GwC_Cy

Cuts at:      0    1297    2031 
   Size:       1297     734
 
PshAI GACnn'nnGTC

Cuts at:      0     772    1246    1859    2031 
   Size:        772     474     613     172

  Fragments arranged by size: 

                 772     613     474     172
 
PsiI TTA'TAA

Cuts at:      0     440    1376    2031 
   Size:        440     936     655

  Fragments arranged by size: 

                 936     655     440
 
PspOMI G'GGCC_C

Cuts at:      0    1939    2031 
   Size:       1939      92
 
PstI C_TGCA'G

Cuts at:      0     686     723    2031 
   Size:        686      37    1308

  Fragments arranged by size: 

                1308     686      37
 
PvuII CAG'CTG

Cuts at:      0     145    2031 
   Size:        145    1886
 
SanDI GG'GwC_CC

Cuts at:      0    1297    2031 
   Size:       1297     734
 
SapI GCTCTTCn'nnn_

Cuts at:      0     281    1805    1904    2031 
   Size:        281    1524      99     127

  Fragments arranged by size: 

                1524     281     127      99
 
SfcI C'TryA_G

Cuts at:      0     682     719    1057    1118    2031 
   Size:        682      37     338      61     913

  Fragments arranged by size: 

                 913     682     338      61      37
 
SmaI CCC'GGG

Cuts at:      0     627    2031 
   Size:        627    1404
 
SmlI C'TyrA_G

Cuts at:      0     311     659    2031 
   Size:        311     348    1372

  Fragments arranged by size: 

                1372     348     311
 
SphI G_CATG'C

Cuts at:      0     994    1287    2031 
   Size:        994     293     744

  Fragments arranged by size: 

                 994     744     293
 
SrfI GCCC'GGGC

Cuts at:      0     627    2031 
   Size:        627    1404
 
StuI AGG'CCT

Cuts at:      0     608    2031 
   Size:        608    1423
 
StyI C'CwwG_G

Cuts at:      0     603    1254    1766    2031 
   Size:        603     651     512     265

  Fragments arranged by size: 

                 651     603     512     265
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1267    2031 
   Size:       1267     764
 
TatI w'GTAC_w

Cuts at:      0    1009    1026    1041    1896    2031 
   Size:       1009      17      15     855     135

  Fragments arranged by size: 

                1009     855     135      17      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     904    2031 
   Size:        904    1127
 
XmaI C'CCGG_G

Cuts at:      0     625    2031 
   Size:        625    1406
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI   AflIII     ApaI     ApoI     AvaI
    BanII     BbsI    BbvCI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrDI    BsrFI    BsrGI   BstAPI   BstEII    BstYI  BstZ17I
     BtgI     BtsI   DraIII     EaeI     EagI     EarI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NsiI     NspI     PciI
    PflMI     PmlI    PpuMI    PshAI     PsiI   PspOMI     PstI    PvuII
    SanDI     SapI     SfcI     SmaI     SmlI     SphI     SrfI     StuI
     StyI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI     BcgI    BciVI     BclI     BglI     BmrI
     BmtI     BsaI    BsaWI    BsiWI    BspEI    BspHI    BsrBI   BssHII
    BssSI    BstBI    BstXI   Bsu36I    BtgZI     ClaI    CspCI     DraI
     DrdI     EciI  EcoICRI    EcoNI    EcoRV     FseI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NheI
     NotI     NruI     PacI     PasI     PfoI     PmeI     PpiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SspI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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