Restriction map for pF1KB6236
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        ApaI        AseI        AsnI        
Asp718I     AvaI        AvrII       BamHI       BbrPI       
BcgI        BclI        BfaI        BfrI        BlnI        
BsaAI       BsaBI       BsaI        BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssSI       Bst1107I    BstBI       BstZ17I     
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        EclXI       Eco52I      EcoRI       
EcoRV       FbaI        FseI        HpaI        KpnI        
MaeI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SapI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI          168  GTCGAC               GT^[AC][GT]AC
AcsI         2005  AAATTC               [GA]^AATT[CT]
AhdI         1588  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          328  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       278  AGCGCT               AGC^GCT
ApoI         2005  AAATTC               [GA]^AATT[CT]
AscI         1923  GGCGCGCC             GG^CGCGCC
Asp700I      1491  GAACATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1588  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          529  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI            3  GGCGCC               GGCGC^C
BbvCI         862  GCTGAGG              CCTCAGC(-5/-2)
BciVI         153  GTATCC               GTATCC(6/5)
BglI         1126  GCCATCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1007  AGATCT               A^GATCT
BlpI          105  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      105  GCTCAGC              GC^T[ACGT]AGC
BsaWI         958  ACCGGA               [AT]^CCGG[AT]
BsmAI        1883  GAGAC                GTCTC(1/5)
BsmI         1975  GCATTC               GAATGC(1/-1)
BspMI         320  ACCTGC               ACCTGC(4/8)
BsrDI         570  GCAATG               GCAATG(2/0)
BssHII       1924  GCGCGC               G^CGCGC
BstEII        249  GGTGACC              G^GT[ACGT]ACC
BstPI         249  GGTGACC              G^GT[ACGT]ACC
Bsu36I        328  CCTCAGG              CC^T[ACGT]AGG
CelII         105  GCTCAGC              GC^T[ACGT]AGC
Eam1105I     1588  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1666  CTCTTC               CTCTTC(1/4)
Eco47III      278  AGCGCT               AGC^GCT
Eco81I        328  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1137  CCTCAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       249  GGTGACC              G^GT[ACGT]ACC
EcoT22I       597  ATGCAT               ATGCA^T
HincII        168  GTCGAC               GT[CT]^[GA]AC
HindII        168  GTCGAC               GT[CT]^[GA]AC
HindIII       853  AAGCTT               A^AGCTT
KasI            3  GGCGCC               G^GCGCC
Ksp632I      1666  CTCTTC               CTCTTC(1/4)
MseI          775  TTAA                 T^TAA
NarI            3  GGCGCC               GG^CGCC
NsiI          597  ATGCAT               ATGCA^T
PflFI         529  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1814  CCATAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI          168  GTCGAC               G^TCGAC
SbfI           48  CCTGCAGG             CCTGCA^GG
ScaI         1118  AGTACT               AGT^ACT
SfoI            3  GGCGCC               GGC^GCC
SmlI         1138  CTCAAG               C^T[CT][GA]AG
Sse8387I       48  CCTGCAGG             CCTGCA^GG
StuI          866  AGGCCT               AGG^CCT
TfiI         1218  GAATC                G^A[AT]TC
Tru9I         775  TTAA                 T^TAA
Tsp509I      2006  AATT                 ^AATT
Tth111I       529  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1814  CCATAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1724  CCAAGGACATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1491  GAACATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI            3  GGCGCC               G[GA]^CG[CT]C
              178  GACGCC              
Alw44I        802  GTGCAC               G^TGCAC
             1176  GTGCAC              
AlwI         1554  GATCC                GGATC(4/5)
             1709  GGATC               
ApaLI         802  GTGCAC               G^TGCAC
             1176  GTGCAC              
AviII          14  TGCGCA               TGC^GCA
              392  TGCGCA              
BanII          42  GGGCTC               G[GA]GC[CT]^C
              717  GAGCCC              
BbsI          784  GAAGAC               GAAGAC(2/6)
              954  GAAGAC              
BpmI          364  CTGGAG               CTGGAG(16/14)
             1735  CTGGAG              
BpuAI         784  GAAGAC               GAAGAC(2/6)
              954  GAAGAC              
BsaHI           3  GGCGCC               G[GA]^CG[CT]C
              178  GACGCC              
BsgI         1242  CTGCAC               GTGCAG(16/14)
              133  GTGCAG              
BstAPI       1022  GCAGCAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1517  GCACCTTCTGC         
BstXI        1676  CCATCCGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1763  CCAAGAGGCTGG        
BstYI        1007  AGATCT               [GA]^GATC[CT]
             1553  AGATCC              
FspI           14  TGCGCA               TGC^GCA
              392  TGCGCA              
HgaI          178  GACGC                GACGC(5/10)
              200  GCGTC               
Hin1I           3  GGCGCC               G[GA]^CG[CT]C
              178  GACGCC              
KspI          432  CCGCGG               CCGC^GG
              632  CCGCGG              
MflI         1007  AGATCT               [GA]^GATC[CT]
             1553  AGATCC              
NspI          683  ACATGC               [GA]CATG^[CT]
              977  ACATGC              
SacII         432  CCGCGG               CCGC^GG
              632  CCGCGG              
XhoII        1007  AGATCT               [GA]^GATC[CT]
             1553  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AspHI       AvaII       BalI        BanI        BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
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