Restriction map for pF1KB6236
 (Linear) (Six-base) MAPSORT of: KIBB6236.seq  Check: 7271  from: 1  to: 2028



KIBB6236.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AccI GT'mk_AC

Cuts at:      0     169    2028 
   Size:        169    1859
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1123    1588    1842    2028 
   Size:       1123     465     254     186

  Fragments arranged by size: 

                1123     465     254     186
 
AfeI AGC'GCT

Cuts at:      0     280    2028 
   Size:        280    1748
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1593    2028 
   Size:       1593     435
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     817    2028 
   Size:        817    1211
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1252    1284    2028 
   Size:       1252      32     744

  Fragments arranged by size: 

                1252     744      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     244     925    1566    2028 
   Size:        244     681     641     462

  Fragments arranged by size: 

                 681     641     462     244
 
ApaLI G'TGCA_C

Cuts at:      0     802    1176    2028 
   Size:        802     374     852

  Fragments arranged by size: 

                 852     802     374
 
ApoI r'AATT_y

Cuts at:      0    2005    2028 
   Size:       2005      23
 
AscI GG'CGCG_CC

Cuts at:      0    1924    2028 
   Size:       1924     104
 
BanI G'GyrC_C

Cuts at:      0       3     628    1528    2028 
   Size:          3     625     900     500

  Fragments arranged by size: 

                 900     625     500       3
 
BanII G_rGCy'C

Cuts at:      0      46     721    2028 
   Size:         46     675    1307

  Fragments arranged by size: 

                1307     675      46
 
BbeI G_GCGC'C

Cuts at:      0       7    2028 
   Size:          7    2021
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     791     961    2028 
   Size:        791     170    1067

  Fragments arranged by size: 

                1067     791     170
 
BbvCI CC'TCA_GC

Cuts at:      0     863    2028 
   Size:        863    1165
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     164    2028 
   Size:        164    1864
 
BfrBI ATG'CAT

Cuts at:      0     599    2028 
   Size:        599    1429
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1132    2028 
   Size:       1132     896
 
BglII A'GATC_T

Cuts at:      0    1007    2028 
   Size:       1007    1021
 
BlpI GC'TnA_GC

Cuts at:      0     106    2028 
   Size:        106    1922
 
Bme1580I G_kGCm'C

Cuts at:      0     806    1180    2028 
   Size:        806     374     848

  Fragments arranged by size: 

                 848     806     374
 
BmgBI CAC'GTC

Cuts at:      0     372    1170    2028 
   Size:        372     798     858

  Fragments arranged by size: 

                 858     798     372
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      60    2028 
   Size:         60    1968
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385    1756    2028 
   Size:        385    1371     272

  Fragments arranged by size: 

                1371     385     272
 
Bpu10I CC'TnA_GC

Cuts at:      0     863    2028 
   Size:        863    1165
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1123    2028 
   Size:       1123     905
 
BsaHI Gr'CG_yC

Cuts at:      0       4     179    2028 
   Size:          4     175    1849

  Fragments arranged by size: 

                1849     175       4
 
BsaWI w'CCGG_w

Cuts at:      0     958    2028 
   Size:        958    1070
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     226     256     898     928    2028 
   Size:        226      30     642      30    1100

  Fragments arranged by size: 

                1100     642     226      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     247     906    1127    2028 
   Size:        247     659     221     901

  Fragments arranged by size: 

                 901     659     247     221
 
BseYI C'CCAG_C

Cuts at:      0      39     967    1770    2028 
   Size:         39     928     803     258

  Fragments arranged by size: 

                 928     803     258      39
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154    1227    2028 
   Size:        154    1073     801

  Fragments arranged by size: 

                1073     801     154
 
BsiHKAI G_wGCw'C

Cuts at:      0     806    1180    2015    2028 
   Size:        806     374     835      13

  Fragments arranged by size: 

                 835     806     374      13
 
BsmI GAATG_Cn'

Cuts at:      0    1975    2028 
   Size:       1975      53
 
Bsp1286I G_dGCh'C

Cuts at:      0      46     721     806    1180    2015    2028 
   Size:         46     675      85     374     835      13

  Fragments arranged by size: 

                 835     675     374      85      46      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     329    2028 
   Size:        329    1699
 
BsrDI GCAATG_nn'

Cuts at:      0     577    2028 
   Size:        577    1451
 
BssHII G'CGCG_C

Cuts at:      0    1924    2028 
   Size:       1924     104
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1028    1523    2028 
   Size:       1028     495     505

  Fragments arranged by size: 

                1028     505     495
 
BstEII G'GTnAC_C

Cuts at:      0     249    2028 
   Size:        249    1779
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1683    1770    2028 
   Size:       1683      87     258

  Fragments arranged by size: 

                1683     258      87
 
BstYI r'GATC_y

Cuts at:      0    1007    1553    2028 
   Size:       1007     546     475

  Fragments arranged by size: 

                1007     546     475
 
Bsu36I CC'TnA_GG

Cuts at:      0     329    2028 
   Size:        329    1699
 
BtgI C'CryG_G

Cuts at:      0     254     432     632    1181    1644    1961    2028 
   Size:        254     178     200     549     463     317      67

  Fragments arranged by size: 

                 549     463     317     254     200     178      67
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0       3    2028 
   Size:          3    2025
 
BtsI GCAGTG_nn'

Cuts at:      0     402     735    2028 
   Size:        402     333    1293

  Fragments arranged by size: 

                1293     402     333
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     477     512    2028 
   Size:        477      35    1516

  Fragments arranged by size: 

                1516     477      35
 
EaeI y'GGCC_r

Cuts at:      0     272     635     827    1452    1629    2028 
   Size:        272     363     192     625     177     399

  Fragments arranged by size: 

                 625     399     363     272     192     177
 
EarI CTCTTCn'nnn_

Cuts at:      0    1672    2028 
   Size:       1672     356
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385    1123    1588    1756    1842    2028 
   Size:        385     738     465     168      86     186

  Fragments arranged by size: 

                 738     465     385     186     168      86
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1141    2028 
   Size:       1141     887
 
EcoO109I rG'GnC_Cy

Cuts at:      0     540     995    1059    1134    1299    1416    1563    1605 
   Size:        540     455      64      75     165     117     147      42

Cuts at:   1605    2028 
   Size:        423

  Fragments arranged by size: 

                 540     455     423     165     147     117      75      64
                  42
 
FspI TGC'GCA

Cuts at:      0      16     394    2028 
   Size:         16     378    1634

  Fragments arranged by size: 

                1634     378      16
 
HaeII r_GCGC'y

Cuts at:      0       7     106     282    2028 
   Size:          7      99     176    1746

  Fragments arranged by size: 

                1746     176      99       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     138     151     170     183     226     258     898     930 
   Size:        138      13      19      13      43      32     640      32

Cuts at:    930    1579    1611    2028 
   Size:        649      32     417

  Fragments arranged by size: 

                 649     640     417     138      43      32      32      32
                  19      13      13
 
HincII GTy'rAC

Cuts at:      0     170    2028 
   Size:        170    1858
 
HindIII A'AGCT_T

Cuts at:      0     853    2028 
   Size:        853    1175
 
KasI G'GCGC_C

Cuts at:      0       3    2028 
   Size:          3    2025
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1421    1826    2028 
   Size:       1421     405     202

  Fragments arranged by size: 

                1421     405     202
 
MscI TGG'CCA

Cuts at:      0     829    1454    1631    2028 
   Size:        829     625     177     397

  Fragments arranged by size: 

                 829     625     397     177
 
MslI CAynn'nnrTG

Cuts at:      0     188     682     799    1649    1681    2028 
   Size:        188     494     117     850      32     347

  Fragments arranged by size: 

                 850     494     347     188     117      32
 
MspA1I CmG'CkG

Cuts at:      0     434     634    1363    1706    2028 
   Size:        434     200     729     343     322

  Fragments arranged by size: 

                 729     434     343     322     200
 
NarI GG'CG_CC

Cuts at:      0       4    2028 
   Size:          4    2024
 
NcoI C'CATG_G

Cuts at:      0    1181    1644    1961    2028 
   Size:       1181     463     317      67

  Fragments arranged by size: 

                1181     463     317      67
 
NsiI A_TGCA'T

Cuts at:      0     601    2028 
   Size:        601    1427
 
NspI r_CATG'y

Cuts at:      0     687     981    2028 
   Size:        687     294    1047

  Fragments arranged by size: 

                1047     687     294
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1820    2028 
   Size:       1820     208
 
PfoI T'CCnGG_A

Cuts at:      0     362     895    1316    1733    2018    2028 
   Size:        362     533     421     417     285      10

  Fragments arranged by size: 

                 533     421     417     362     285      10
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     397     429    2028 
   Size:        397      32    1599

  Fragments arranged by size: 

                1599     397      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     995    1059    1299    1416    1605    2028 
   Size:        995      64     240     117     189     423

  Fragments arranged by size: 

                 995     423     240     189     117      64
 
PstI C_TGCA'G

Cuts at:      0      53     326    1385    2028 
   Size:         53     273    1059     643

  Fragments arranged by size: 

                1059     643     273      53
 
SacII CC_GC'GG

Cuts at:      0     435     635    2028 
   Size:        435     200    1393

  Fragments arranged by size: 

                1393     435     200
 
SalI G'TCGA_C

Cuts at:      0     168    2028 
   Size:        168    1860
 
SbfI CC_TGCA'GG

Cuts at:      0      53    2028 
   Size:         53    1975
 
ScaI AGT'ACT

Cuts at:      0    1120    2028 
   Size:       1120     908
 
SfcI C'TryA_G

Cuts at:      0      49     322    1381    1558    1887    1935    2028 
   Size:         49     273    1059     177     329      48      93

  Fragments arranged by size: 

                1059     329     273     177      93      49      48
 
SfoI GGC'GCC

Cuts at:      0       5    2028 
   Size:          5    2023
 
SmlI C'TyrA_G

Cuts at:      0    1138    2028 
   Size:       1138     890
 
StuI AGG'CCT

Cuts at:      0     868    2028 
   Size:        868    1160
 
StyI C'CwwG_G

Cuts at:      0     335    1181    1256    1644    1715    1724    1961    2028 
   Size:        335     846      75     388      71       9     237      67

  Fragments arranged by size: 

                 846     388     335     237      75      71      67       9
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     247     898    1246    1893    2028 
   Size:        247     651     348     647     135

  Fragments arranged by size: 

                 651     647     348     247     135
 
TatI w'GTAC_w

Cuts at:      0    1118    1571    2028 
   Size:       1118     453     457

  Fragments arranged by size: 

                1118     457     453
 
Tth111I GACn'n_nGTC

Cuts at:      0     532    2028 
   Size:        532    1496
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1731    2028 
   Size:       1731     297
 
XmnI GAAnn'nnTTC

Cuts at:      0    1495    2028 
   Size:       1495     533
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     AhdI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BplI     BpmI
   Bpu10I    BpuEI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI   BssHII   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     EaeI
     EarI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI
     NsiI     NspI    PflMI     PfoI     PpiI    PpuMI     PstI    SacII
     SalI     SbfI     ScaI     SfcI     SfoI     SmlI     StuI     StyI
    TaqII     TatI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AleI
     ApaI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BcgI
     BclI     BmrI     BmtI     BsaI    BsaAI    BsaBI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI    BssSI    BstBI
  BstZ17I     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoRI    EcoRV     FalI     FseI    FspAI     HpaI     KpnI     MfeI
     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SanDI     SapI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TstI     XbaI     XhoI     XmaI     ZraI
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