Restriction map for
pF1KB6215
(Circular) (Six-base) MAPSORT of: pF1KB6215.seq Check: 1964 from: 1 to: 5120
pF1KB6215 5120 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 2158 2158
Size: 5120
Acc65I G'GTAC_C
Cuts at: 578 1018 1604 2136 578
Size: 440 586 532 3562
Fragments arranged by size:
3562 586 532 440
AclI AA'CG_TT
Cuts at: 4960 4960
Size: 5120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2945 3377 4124 4392 2945
Size: 432 747 268 3673
Fragments arranged by size:
3673 747 432 268
AfeI AGC'GCT
Cuts at: 370 499 2095 370
Size: 129 1596 3395
Fragments arranged by size:
3395 1596 129
AflIII A'CryG_T
Cuts at: 2073 2472 3576 4553 2073
Size: 399 1104 977 2640
Fragments arranged by size:
2640 1104 977 399
AgeI A'CCGG_T
Cuts at: 391 751 3508 391
Size: 360 2757 2003
Fragments arranged by size:
2757 2003 360
AleI CACnn'nnGTG
Cuts at: 394 1850 394
Size: 1456 3664
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 865 865
Size: 5120
AlwNI CAG_nnn'CTG
Cuts at: 1467 1565 1625 2425 3992 1467
Size: 98 60 800 1567 2595
Fragments arranged by size:
2595 1567 800 98 60
ApaI G_GGCC'C
Cuts at: 1162 1162
Size: 5120
ApaLI G'TGCA_C
Cuts at: 3890 3890
Size: 5120
ApoI r'AATT_y
Cuts at: 1714 2124 4473 4729 1714
Size: 410 2349 256 2105
Fragments arranged by size:
2349 2105 410 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5120
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
AvaI C'yCGr_G
Cuts at: 120 982 1175 1861 2140 120
Size: 862 193 686 279 3100
Fragments arranged by size:
3100 862 686 279 193
AvrII C'CTAG_G
Cuts at: 792 792
Size: 5120
BamHI G'GATC_C
Cuts at: 380 1634 2145 380
Size: 1254 511 3355
Fragments arranged by size:
3355 1254 511
BanI G'GyrC_C
Cuts at: 444 578 651 748 939 1018 1604 1699 1998
Size: 134 73 97 191 79 586 95 299
Cuts at: 1998 2136 2550 2798 2833 444
Size: 138 414 248 35 2731
Fragments arranged by size:
2731 586 414 299 248 191 138 134
97 95 79 73 35
BanII G_rGCy'C
Cuts at: 827 1162 1451 1529 1745 1862 2134 3164 827
Size: 335 289 78 216 117 272 1030 2783
Fragments arranged by size:
2783 1030 335 289 272 216 117 78
BbeI G_GCGC'C
Cuts at: 943 2554 2802 943
Size: 1611 248 3261
Fragments arranged by size:
3261 1611 248
BbsI GAAGACnn'nnnn_
Cuts at: 254 633 254
Size: 379 4741
BbvCI CC'TCA_GC
Cuts at: 299 299
Size: 5120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 141 175 141
Size: 34 5086
BciVI GTATCCnnnnn_n'
Cuts at: 3018 3779 5120 3018
Size: 761 1341 3018
Fragments arranged by size:
3018 1341 761
BclI T'GATC_A
Cuts at: 2640 2640
Size: 5120
BglI GCCn_nnn'nGGC
Cuts at: 135 161 937 1074 1083 1104 1143 1332 135
Size: 26 776 137 9 21 39 189 3923
Fragments arranged by size:
3923 776 189 137 39 26 21 9
BglII A'GATC_T
Cuts at: 338 2635 4672 338
Size: 2297 2037 786
Fragments arranged by size:
2297 2037 786
BlpI GC'TnA_GC
Cuts at: 2232 2232
Size: 5120
Bme1580I G_kGCm'C
Cuts at: 656 1162 1337 1702 2745 2838 3894 656
Size: 506 175 365 1043 93 1056 1882
Fragments arranged by size:
1882 1056 1043 506 365 175 93
BmgBI CAC'GTC
Cuts at: 1821 1887 1821
Size: 66 5054
BmrI ACTGGGnnnn_n'
Cuts at: 379 1159 2332 2413 2748 4892 379
Size: 780 1173 81 335 2144 607
Fragments arranged by size:
2144 1173 780 607 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3487 3487
Size: 5120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 822 900 928 1465 822
Size: 78 28 537 4477
Fragments arranged by size:
4477 537 78 28
Bpu10I CC'TnA_GC
Cuts at: 299 1663 299
Size: 1364 3756
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2296 3175 3667 3965 2296
Size: 879 492 298 3451
Fragments arranged by size:
3451 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 403 1589 4824 403
Size: 1186 3235 699
Fragments arranged by size:
3235 1186 699
BsaAI yAC'GTr
Cuts at: 1794 2074 2473 1794
Size: 280 399 4441
Fragments arranged by size:
4441 399 280
BsaBI GATnn'nnATC
Cuts at: 6 2634 4671 6
Size: 2628 2037 455
Fragments arranged by size:
2628 2037 455
BsaHI Gr'CG_yC
Cuts at: 489 940 1066 2551 2799 3501 4997 489
Size: 451 126 1485 248 702 1496 612
Fragments arranged by size:
1496 1485 702 612 451 248 126
BsaWI w'CCGG_w
Cuts at: 391 751 2309 2830 3508 3782 3929 4340 391
Size: 360 1558 521 678 274 147 411 1171
Fragments arranged by size:
1558 1171 678 521 411 360 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 187 1594 1975 148
Size: 39 1407 381 3293
Fragments arranged by size:
3293 1407 381 39
BseYI C'CCAG_C
Cuts at: 304 888 900 1046 1112 2545 3303 3880 4376
Size: 584 12 146 66 1433 758 577 496
Cuts at: 4376 304
Size: 1048
Fragments arranged by size:
1433 1048 758 584 577 496 146 66
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 1972 2068 1874
Size: 98 96 4926
Fragments arranged by size:
4926 98 96
BsiEI CG_ry'CG
Cuts at: 104 139 168 187 245 1099 1392 1831 2662
Size: 35 29 19 58 854 293 439 831
Cuts at: 2662 2708 3916 104
Size: 46 1208 1308
Fragments arranged by size:
1308 1208 854 831 439 293 58 46
35 29 19
BsiHKAI G_wGCw'C
Cuts at: 401 914 1793 2134 2912 3102 3894 401
Size: 513 879 341 778 190 792 1627
Fragments arranged by size:
1627 879 792 778 513 341 190
Bsp1286I G_dGCh'C
Cuts at: 401 656 827 914 1162 1337 1451 1529 1702
Size: 255 171 87 248 175 114 78 173
Cuts at: 1702 1745 1793 1862 2134 2745 2838 2912 3102
Size: 43 48 69 272 611 93 74 190
Cuts at: 3102 3164 3894 401
Size: 62 730 1627
Fragments arranged by size:
1627 730 611 272 255 248 190 175
173 171 114 93 87 78 74 69
62 48 43
BspEI T'CCGG_A
Cuts at: 4340 4340
Size: 5120
BspHI T'CATG_A
Cuts at: 4296 4296
Size: 5120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 302 488 857 2170 2384 2686 3067 302
Size: 186 369 1313 214 302 381 2355
Fragments arranged by size:
2355 1313 381 369 302 214 186
BsrBI CCG'CTC
Cuts at: 50 1143 1188 3412 3466 4950 5115 50
Size: 1093 45 2224 54 1484 165 55
Fragments arranged by size:
2224 1484 1093 165 55 54 45
BsrDI GCAATG_nn'
Cuts at: 1674 3032 1674
Size: 1358 3762
BsrFI r'CCGG_y
Cuts at: 391 751 1082 1094 3118 3299 3508 391
Size: 360 331 12 2024 181 209 2003
Fragments arranged by size:
2024 2003 360 331 209 181 12
BssHII G'CGCG_C
Cuts at: 125 125
Size: 5120
BssSI C'ACGA_G
Cuts at: 3391 3749 3391
Size: 358 4762
BstAPI GCAn_nnn'nTGC
Cuts at: 2497 2497
Size: 5120
BstBI TT'CG_AA
Cuts at: 3481 3481
Size: 5120
BstEII G'GTnAC_C
Cuts at: 1411 1411
Size: 5120
BstXI CCAn_nnnn'nTGG
Cuts at: 636 636
Size: 5120
BstYI r'GATC_y
Cuts at: 1 338 380 1634 2145 2612 2635 2970 3216
Size: 337 42 1254 511 467 23 335 246
Cuts at: 3216 4228 4314 4326 4672 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1254 1012 511 467 449 346 337 335
246 86 42 23 12
BtgI C'CryG_G
Cuts at: 107 723 996 1069 1845 107
Size: 616 273 73 776 3382
Fragments arranged by size:
3382 776 616 273 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3070 3251 3414 3070
Size: 181 163 4776
Fragments arranged by size:
4776 181 163
BtsI GCAGTG_nn'
Cuts at: 884 2002 2363 884
Size: 1118 361 3641
Fragments arranged by size:
3641 1118 361
DraI TTT'AAA
Cuts at: 2119 2119
Size: 5120
DraIII CAC_nnn'GTG
Cuts at: 1591 1591
Size: 5120
DrdI GACnn_nn'nnGTC
Cuts at: 256 2826 3684 256
Size: 2570 858 1692
Fragments arranged by size:
2570 1692 858
EaeI y'GGCC_r
Cuts at: 136 184 242 1075 1096 1238 1511 1828 2705
Size: 48 58 833 21 142 273 317 877
Cuts at: 2705 2879 3270 3297 4642 136
Size: 174 391 27 1345 614
Fragments arranged by size:
1345 877 833 614 391 317 273 174
142 58 48 27 21
EagI C'GGCC_G
Cuts at: 136 184 242 1096 1828 2705 136
Size: 48 58 854 732 877 2551
Fragments arranged by size:
2551 877 854 732 58 48
EarI CTCTTCn'nnn_
Cuts at: 401 871 3143 3353 401
Size: 470 2272 210 2168
Fragments arranged by size:
2272 2168 470 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 321 1882 3328 3638 3784 4930 321
Size: 1561 1446 310 146 1146 511
Fragments arranged by size:
1561 1446 1146 511 310 146
EcoICRI GAG'CTC
Cuts at: 2132 2132
Size: 5120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 822 900 928 1465 2945 3377 4124 4392 822
Size: 78 28 537 1480 432 747 268 1550
Fragments arranged by size:
1550 1480 747 537 432 268 78 28
EcoO109I rG'GnC_Cy
Cuts at: 605 1159 1655 1804 2259 605
Size: 554 496 149 455 3466
Fragments arranged by size:
3466 554 496 455 149
EcoRI G'AATT_C
Cuts at: 2124 2124
Size: 5120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3139 3139
Size: 5120
FseI GG_CCGG'CC
Cuts at: 1086 1086
Size: 5120
FspI TGC'GCA
Cuts at: 601 2901 601
Size: 2300 2820
HaeII r_GCGC'y
Cuts at: 372 501 943 1383 1572 1731 2097 2554 2802
Size: 129 442 440 189 159 366 457 248
Cuts at: 2802 3824 4803 372
Size: 1022 979 689
Fragments arranged by size:
1022 979 689 457 442 440 366 248
189 159 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2962 2992 2994 3024 2962
Size: 30 2 30 5058
Fragments arranged by size:
5058 30 30 2
HincII GTy'rAC
Cuts at: 966 1923 2159 966
Size: 957 236 3927
Fragments arranged by size:
3927 957 236
KasI G'GCGC_C
Cuts at: 939 2550 2798 939
Size: 1611 248 3261
Fragments arranged by size:
3261 1611 248
KpnI G_GTAC'C
Cuts at: 582 1022 1608 2140 582
Size: 440 586 532 3562
Fragments arranged by size:
3562 586 532 440
MluI A'CGCG_T
Cuts at: 4553 4553
Size: 5120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 472 3791 3975 472
Size: 3319 184 1617
Fragments arranged by size:
3319 1617 184
MscI TGG'CCA
Cuts at: 1240 2881 1240
Size: 1641 3479
MslI CAynn'nnrTG
Cuts at: 394 1850 3109 3236 394
Size: 1456 1259 127 2278
Fragments arranged by size:
2278 1456 1259 127
MspA1I CmG'CkG
Cuts at: 201 437 1277 1467 2231 2545 2905 3918 4163
Size: 236 840 190 764 314 360 1013 245
Cuts at: 4163 201
Size: 1158
Fragments arranged by size:
1158 1013 840 764 360 314 245 236
190
NaeI GCC'GGC
Cuts at: 1084 3301 1084
Size: 2217 2903
NarI GG'CG_CC
Cuts at: 940 2551 2799 940
Size: 1611 248 3261
Fragments arranged by size:
3261 1611 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5120
NdeI CA'TA_TG
Cuts at: 314 314
Size: 5120
NgoMIV G'CCGG_C
Cuts at: 1082 3299 1082
Size: 2217 2903
NotI GC'GGCC_GC
Cuts at: 184 184
Size: 5120
NspI r_CATG'y
Cuts at: 2173 2673 3580 2173
Size: 500 907 3713
Fragments arranged by size:
3713 907 500
PasI CC'CwG_GG
Cuts at: 1600 1600
Size: 5120
PciI A'CATG_T
Cuts at: 3576 3576
Size: 5120
PflMI CCAn_nnn'nTGG
Cuts at: 342 419 588 900 1520 1709 342
Size: 77 169 312 620 189 3753
Fragments arranged by size:
3753 620 312 189 169 77
PfoI T'CCnGG_A
Cuts at: 644 1307 644
Size: 663 4457
PmeI GTTT'AAAC
Cuts at: 2119 2119
Size: 5120
PmlI CAC'GTG
Cuts at: 1794 2074 1794
Size: 280 4840
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 4259
Size: 32 5088
PspOMI G'GGCC_C
Cuts at: 1158 1158
Size: 5120
PstI C_TGCA'G
Cuts at: 2105 2167 2852 2105
Size: 62 685 4373
Fragments arranged by size:
4373 685 62
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5120
PvuII CAG'CTG
Cuts at: 2545 2905 2545
Size: 360 4760
RsrII CG'GwC_CG
Cuts at: 3315 3315
Size: 5120
SacI G_AGCT'C
Cuts at: 2134 2134
Size: 5120
SalI G'TCGA_C
Cuts at: 2157 2157
Size: 5120
SapI GCTCTTCn'nnn_
Cuts at: 3143 3353 3143
Size: 210 4910
SbfI CC_TGCA'GG
Cuts at: 2167 2167
Size: 5120
ScaI AGT'ACT
Cuts at: 4637 4637
Size: 5120
SfcI C'TryA_G
Cuts at: 33 2101 2163 2848 3841 4032 33
Size: 2068 62 685 993 191 1121
Fragments arranged by size:
2068 1121 993 685 191 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 1083 1104 1083
Size: 21 5099
SfoI GGC'GCC
Cuts at: 941 2552 2800 941
Size: 1611 248 3261
Fragments arranged by size:
3261 1611 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
SgrAI Cr'CCGG_yG
Cuts at: 391 751 391
Size: 360 4760
SmaI CCC'GGG
Cuts at: 1863 2142 1863
Size: 279 4841
SmlI C'TyrA_G
Cuts at: 2275 3190 3682 3944 2275
Size: 915 492 262 3451
Fragments arranged by size:
3451 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5120
SphI G_CATG'C
Cuts at: 2173 2673 2173
Size: 500 4620
StyI C'CwwG_G
Cuts at: 107 231 413 792 2254 2608 107
Size: 124 182 379 1462 354 2619
Fragments arranged by size:
2619 1462 379 354 182 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 820 2703 2836 3295 3504 820
Size: 1883 133 459 209 2436
Fragments arranged by size:
2436 1883 459 209 133
TatI w'GTAC_w
Cuts at: 4635 4635
Size: 5120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4637 4669 4637
Size: 32 5088
Tth111I GACn'n_nGTC
Cuts at: 2917 2917
Size: 5120
XbaI T'CTAG_A
Cuts at: 2151 2151
Size: 5120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 933 1479 1808 2615 933
Size: 546 329 807 3438
Fragments arranged by size:
3438 807 546 329
XmaI C'CCGG_G
Cuts at: 1861 2140 1861
Size: 279 4841
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI BaeI BfrBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FspAI HindIII HpaI MfeI
NheI NruI NsiI PacI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI