Restriction map for pF1KB6830
##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Aor51HI     ApaI        
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaII       AviII       
AvrII       BalI        BanI        BanII       BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SphI        Sse8387I    SspBI       SspI        
SwaI        TaqI        Tru9I       Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

Alw44I        137  GTGCAC               G^TGCAC
AlwNI         283  CAGGGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          409  CCTGAGG              CC^T[ACGT]AGG
ApaLI         137  GTGCAC               G^TGCAC
AspHI         137  GTGCAC               G[AT]GC[AT]^C
AvaI          530  CCCGGG               C^[CT]CG[GA]G
BamHI         275  GGATCC               G^GATCC
BbvCI         390  CCTCAGC              CCTCAGC(-5/-2)
BmyI          137  GTGCAC               G[AGT]GC[ACT]^C
BsgI          120  CTGCAC               GTGCAG(16/14)
BsiHKAI       137  GTGCAC               G[AT]GC[AT]^C
BsiYI         145  CCACTAGTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          145  CCACTAGTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI         472  CGTCTC               CGTCTC(1/5)
BsoBI         530  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      137  GTGCAC               G[AGT]GC[ACT]^C
BstEII        415  GGTCACC              G^GT[ACGT]ACC
BstF5I        142  CATCC                GGATG(2/0)
BstPI         415  GGTCACC              G^GT[ACGT]ACC
Bsu36I        409  CCTGAGG              CC^T[ACGT]AGG
Eco81I        409  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       415  GGTCACC              G^GT[ACGT]ACC
FokI          142  CATCC                GGATG(9/13)
HapII         531  CCGG                 C^CGG
HgaI          471  GCGTC                GACGC(5/10)
HpaII         531  CCGG                 C^CGG
HphI          417  TCACC                GGTGA(8/7)
MspI          531  CCGG                 C^CGG
PshAI         250  GACAACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          403  CTGCAG               CTGCA^G
SmaI          530  CCCGGG               CCC^GGG
SpeI          147  ACTAGT               A^CTAGT
StuI          509  AGGCCT               AGG^CCT
TfiI          438  GATTC                G^A[AT]TC
XmaCI         530  CCCGGG               C^CCGGG
XmaI          530  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AciI          483  CCGC                 CCGC(-3/-1)
              527  CCGC                
BcnI          530  CCCGG                CC^[GC]GG
              531  CCGGG               
BfaI          148  CTAG                 C^TAG
              310  CTAG                
BglI          356  GCCCTGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              511  GCCTTTGTGGC         
BpmI          505  CTCCAG               CTGGAG(16/14)
              132  CTGGAG              
BstYI         162  GGATCT               [GA]^GATC[CT]
              275  GGATCC              
Cfr13I        363  GGGCC                G^G[ACGT]CC
              387  GGGCC               
DsaI          463  CCATGG               C^C[GA][CT]GG
              543  CCATGG              
EarI          172  GAAGAG               CTCTTC(1/4)
              184  GAAGAG              
Ksp632I       172  GAAGAG               CTCTTC(1/4)
              184  GAAGAG              
MaeI          148  CTAG                 C^TAG
              310  CTAG                
MaeIII        230  GTGAC                ^GT[ACGT]AC
              416  GTCAC               
MboII         172  GAAGA                GAAGA(8/7)
              184  GAAGA               
MflI          162  GGATCT               [GA]^GATC[CT]
              275  GGATCC              
NciI          530  CCCGG                CC^[GC]GG
              531  CCGGG               
NcoI          463  CCATGG               C^CATGG
              543  CCATGG              
NlaIV         275  GGATCC               GG[ACGT]^[ACGT]CC
              333  GGAGCC              
Sau96I        363  GGGCC                G^G[ACGT]CC
              387  GGGCC               
SfcI          403  CTGCAG               C^T[GA][CT]AG
              521  CTGTAG              
Tsp45I        230  GTGAC                ^GT[GC]AC
              416  GTCAC               
XcmI          207  CCATATCCAGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              213  CCAGTGTGGAGATGG     
XhoII         162  GGATCT               [GA]^GATC[CT]
              275  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AluI        AlwI        BbvI        BsaJI       BsmAI       
BsmFI       BsrI        BssKI       BstNI       Cac8I       
DdeI        DpnII       EcoRII      EcoT14I     Fnu4HI      
HaeIII      HinfI       ItaI        MboI        MnlI        
MvaI        MwoI        NdeII       NlaIII      PleI        
Sau3AI      ScrFI       StyI        TseI        TspRI       
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