Restriction map for pF1KB6786
##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BcgI        
BciVI       BclI        BcnI        BfrI        BglII       
BlnI        BpmI        BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      Cfr10I      
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HpaII       HphI        KasI        
KpnI        KspI        MaeIII      MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        Sau3AI      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tsp45I      Tth111I     XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AciI           37  CCGC                 CCGC(-3/-1)
AflIII        240  ACACGT               A^C[GA][CT]GT
AspHI         244  GTGCTC               G[AT]GC[AT]^C
AvaII          66  GGACC                G^G[AT]CC
BbrPI         241  CACGTG               CAC^GTG
BbsI          279  GAAGAC               GAAGAC(2/6)
BbvCI         286  GCTGAGG              CCTCAGC(-5/-2)
BfaI           43  CTAG                 C^TAG
BglI          430  GCCCTGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          100  GCTCAGC              GC^T[ACGT]AGC
BmyI          244  GTGCTC               G[AGT]GC[ACT]^C
Bpu1102I      100  GCTCAGC              GC^T[ACGT]AGC
BpuAI         279  GAAGAC               GAAGAC(2/6)
BsaAI         241  CACGTG               [CT]AC^GT[GA]
BsiHKAI       244  GTGCTC               G[AT]GC[AT]^C
BsiYI          69  CCAGGAAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           69  CCAGGAAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      244  GTGCTC               G[AGT]GC[ACT]^C
BspDI         250  ATCGAT               AT^CGAT
BsrDI          96  CATTGC               GCAATG(2/0)
BsrI          262  ACTGG                ACTGG(1/-1)
BstF5I        291  GGATG                GGATG(2/0)
Cac8I         372  GCACGC               GC[ACGT]^[ACGT]GC
CelII         100  GCTCAGC              GC^T[ACGT]AGC
CfoI            4  GCGC                 GCG^C
Cfr13I         66  GGACC                G^G[ACGT]CC
ClaI          250  ATCGAT               AT^CGAT
EarI          359  CTCTTC               CTCTTC(1/4)
FokI          291  GGATG                GGATG(9/13)
HhaI            4  GCGC                 GCG^C
HinP1I          4  GCGC                 G^CGC
Ksp632I       359  CTCTTC               CTCTTC(1/4)
MaeI           43  CTAG                 C^TAG
MseI           14  TTAA                 T^TAA
PflMI          69  CCAGGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          405  GAGTC                GAGTC(4/5)
PmaCI         241  CACGTG               CAC^GTG
PmlI          241  CACGTG               CAC^GTG
SapI          358  GCTCTTC              GCTCTTC(1/4)
Sau96I         66  GGACC                G^G[ACGT]CC
SfaNI         292  GATGC                GCATC(5/9)
SmlI           52  CTCAAG               C^T[CT][GA]AG
TaqI          251  TCGA                 T^CGA
Tru9I          14  TTAA                 T^TAA
Tsp509I       182  AATT                 ^AATT
TspRI         194  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       251  TCGA                 T^CGA
Van91I         69  CCAGGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AfaI          115  GTAC                 GT^AC
              267  GTAC                
BsmFI         190  GTCCC                GGGAC(10/14)
              214  GTCCC               
BstXI         122  CCAAGTATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              332  CCAAGCAGATGG        
EcoT14I       137  CCAAGG               C^C[AT][AT]GG
              296  CCAAGG              
HaeIII        387  GGCC                 GG^CC
              459  GGCC                
MslI          249  CATCGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              393  CATGGAAATG          
MspA1I         61  CAGCTG               C[AC]G^C[GT]G
              284  CAGCTG              
PvuII          61  CAGCTG               CAG^CTG
              284  CAGCTG              
RsaI          115  GTAC                 GT^AC
              267  GTAC                
StyI          137  CCAAGG               C^C[AT][AT]GG
              296  CCAAGG              
TfiI           30  GAATC                G^A[AT]TC
              414  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AluI        BbvI        BsaJI       BssKI       BstNI       
DdeI        EcoRII      Fnu4HI      HinfI       ItaI        
MaeII       MboII       MnlI        MvaI        MwoI        
NlaIII      ScrFI       TseI        
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