Restriction map for
pF1KB6786
(Circular) (Six-base) MAPSORT of: pF1KB6786.seq Check: 8981 from: 1 to: 3575
pF1KB6786 3575 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 613 613
Size: 3575
Acc65I G'GTAC_C
Cuts at: 591 591
Size: 3575
AclI AA'CG_TT
Cuts at: 3415 3415
Size: 3575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 1400 1832 2579 2847 454
Size: 946 432 747 268 1182
Fragments arranged by size:
1182 946 747 432 268
AflIII A'CryG_T
Cuts at: 348 927 2031 3008 348
Size: 579 1104 977 915
Fragments arranged by size:
1104 977 915 579
AgeI A'CCGG_T
Cuts at: 1963 1963
Size: 3575
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 172 204 172
Size: 32 3543
AlwNI CAG_nnn'CTG
Cuts at: 880 2447 880
Size: 1567 2008
ApaLI G'TGCA_C
Cuts at: 2345 2345
Size: 3575
ApoI r'AATT_y
Cuts at: 579 2928 3184 579
Size: 2349 256 970
Fragments arranged by size:
2349 970 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3575
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
AvaI C'yCGr_G
Cuts at: 595 595
Size: 3575
BamHI G'GATC_C
Cuts at: 600 600
Size: 3575
BanI G'GyrC_C
Cuts at: 591 1005 1253 1288 591
Size: 414 248 35 2878
Fragments arranged by size:
2878 414 248 35
BanII G_rGCy'C
Cuts at: 589 1619 589
Size: 1030 2545
BbeI G_GCGC'C
Cuts at: 1009 1257 1009
Size: 248 3327
BbsI GAAGACnn'nnnn_
Cuts at: 394 394
Size: 3575
BbvCI CC'TCA_GC
Cuts at: 395 395
Size: 3575
BciVI GTATCCnnnnn_n'
Cuts at: 1473 2234 3575 1473
Size: 761 1341 1473
Fragments arranged by size:
1473 1341 761
BclI T'GATC_A
Cuts at: 1095 1095
Size: 3575
BglI GCCn_nnn'nGGC
Cuts at: 544 544
Size: 3575
BglII A'GATC_T
Cuts at: 1090 3127 1090
Size: 2037 1538
BlpI GC'TnA_GC
Cuts at: 209 687 209
Size: 478 3097
Bme1580I G_kGCm'C
Cuts at: 1200 1293 2349 1200
Size: 93 1056 2426
Fragments arranged by size:
2426 1056 93
BmgBI CAC'GTC
Cuts at: 200 200
Size: 3575
BmrI ACTGGGnnnn_n'
Cuts at: 380 787 868 1203 3347 380
Size: 407 81 335 2144 608
Fragments arranged by size:
2144 608 407 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3575
Bpu10I CC'TnA_GC
Cuts at: 395 551 563 395
Size: 156 12 3407
Fragments arranged by size:
3407 156 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 751 1630 2122 2420 145
Size: 606 879 492 298 1300
Fragments arranged by size:
1300 879 606 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3279 3279
Size: 3575
BsaAI yAC'GTr
Cuts at: 351 928 351
Size: 577 2998
BsaBI GATnn'nnATC
Cuts at: 6 1089 3126 6
Size: 1083 2037 455
Fragments arranged by size:
2037 1083 455
BsaHI Gr'CG_yC
Cuts at: 1006 1254 1956 3452 1006
Size: 248 702 1496 1129
Fragments arranged by size:
1496 1129 702 248
BsaWI w'CCGG_w
Cuts at: 764 1285 1963 2237 2384 2795 764
Size: 521 678 274 147 411 1544
Fragments arranged by size:
1544 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 178 208 178
Size: 30 3545
BseYI C'CCAG_C
Cuts at: 1000 1758 2335 2831 1000
Size: 758 577 496 1744
Fragments arranged by size:
1744 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1117 1163 2371 104
Size: 1013 46 1208 1308
Fragments arranged by size:
1308 1208 1013 46
BsiHKAI G_wGCw'C
Cuts at: 356 589 1367 1557 2349 356
Size: 233 778 190 792 1582
Fragments arranged by size:
1582 792 778 233 190
Bsp1286I G_dGCh'C
Cuts at: 356 589 1200 1293 1367 1557 1619 2349 356
Size: 233 611 93 74 190 62 730 1582
Fragments arranged by size:
1582 730 611 233 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2795 2795
Size: 3575
BspHI T'CATG_A
Cuts at: 2751 2751
Size: 3575
BspMI ACCTGCnnnn'nnnn_
Cuts at: 625 839 1141 1522 625
Size: 214 302 381 2678
Fragments arranged by size:
2678 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1867 1921 3405 3570 50
Size: 1817 54 1484 165 55
Fragments arranged by size:
1817 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 203 1487 203
Size: 1284 2291
BsrFI r'CCGG_y
Cuts at: 1573 1754 1963 1573
Size: 181 209 3185
Fragments arranged by size:
3185 209 181
BssSI C'ACGA_G
Cuts at: 1846 2204 1846
Size: 358 3217
BstAPI GCAn_nnn'nTGC
Cuts at: 952 952
Size: 3575
BstBI TT'CG_AA
Cuts at: 1936 1936
Size: 3575
BstXI CCAn_nnnn'nTGG
Cuts at: 237 447 237
Size: 210 3365
BstYI r'GATC_y
Cuts at: 1 600 1067 1090 1425 1671 2683 2769 2781
Size: 599 467 23 335 246 1012 86 12
Cuts at: 2781 3127 1
Size: 346 449
Fragments arranged by size:
1012 599 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3575
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1525 1706 1869 1525
Size: 181 163 3231
Fragments arranged by size:
3231 181 163
BtsI GCAGTG_nn'
Cuts at: 818 818
Size: 3575
ClaI AT'CG_AT
Cuts at: 359 359
Size: 3575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 186 221 186
Size: 35 3540
DraI TTT'AAA
Cuts at: 574 574
Size: 3575
DrdI GACnn_nn'nnGTC
Cuts at: 1281 2139 1281
Size: 858 2717
EaeI y'GGCC_r
Cuts at: 1160 1334 1725 1752 3097 1160
Size: 174 391 27 1345 1638
Fragments arranged by size:
1638 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1160 1160
Size: 3575
EarI CTCTTCn'nnn_
Cuts at: 473 1598 1808 473
Size: 1125 210 2240
Fragments arranged by size:
2240 1125 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1783 2093 2239 3385 1783
Size: 310 146 1146 1973
Fragments arranged by size:
1973 1146 310 146
EcoICRI GAG'CTC
Cuts at: 587 587
Size: 3575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 1400 1832 2579 2847 454
Size: 946 432 747 268 1182
Fragments arranged by size:
1182 946 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 714 714
Size: 3575
EcoRI G'AATT_C
Cuts at: 579 579
Size: 3575
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 429 1594 429
Size: 1165 2410
FspI TGC'GCA
Cuts at: 1356 1356
Size: 3575
HaeII r_GCGC'y
Cuts at: 1009 1257 2279 3258 1009
Size: 248 1022 979 1326
Fragments arranged by size:
1326 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 1417 1447 1449 1479 178
Size: 32 1207 30 2 30 2274
Fragments arranged by size:
2274 1207 32 30 30 2
HincII GTy'rAC
Cuts at: 614 614
Size: 3575
KasI G'GCGC_C
Cuts at: 1005 1253 1005
Size: 248 3327
KpnI G_GTAC'C
Cuts at: 595 595
Size: 3575
MluI A'CGCG_T
Cuts at: 3008 3008
Size: 3575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 483 2246 2430 483
Size: 1763 184 1628
Fragments arranged by size:
1763 1628 184
MscI TGG'CCA
Cuts at: 1336 1336
Size: 3575
MslI CAynn'nnrTG
Cuts at: 361 505 1564 1691 361
Size: 144 1059 127 2245
Fragments arranged by size:
2245 1059 144 127
MspA1I CmG'CkG
Cuts at: 171 394 686 1000 1360 2373 2618 171
Size: 223 292 314 360 1013 245 1128
Fragments arranged by size:
1128 1013 360 314 292 245 223
NaeI GCC'GGC
Cuts at: 1756 1756
Size: 3575
NarI GG'CG_CC
Cuts at: 1006 1254 1006
Size: 248 3327
NcoI C'CATG_G
Cuts at: 107 107
Size: 3575
NgoMIV G'CCGG_C
Cuts at: 1754 1754
Size: 3575
NspI r_CATG'y
Cuts at: 628 1128 2035 628
Size: 500 907 2168
Fragments arranged by size:
2168 907 500
PasI CC'CwG_GG
Cuts at: 325 540 325
Size: 215 3360
PciI A'CATG_T
Cuts at: 2031 2031
Size: 3575
PflMI CCAn_nnn'nTGG
Cuts at: 183 183
Size: 3575
PmeI GTTT'AAAC
Cuts at: 574 574
Size: 3575
PmlI CAC'GTG
Cuts at: 351 351
Size: 3575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 338 370 2714 2746 338
Size: 32 2344 32 1167
Fragments arranged by size:
2344 1167 32 32
PstI C_TGCA'G
Cuts at: 622 1307 622
Size: 685 2890
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3575
PvuII CAG'CTG
Cuts at: 171 394 1000 1360 171
Size: 223 606 360 2386
Fragments arranged by size:
2386 606 360 223
RsrII CG'GwC_CG
Cuts at: 1770 1770
Size: 3575
SacI G_AGCT'C
Cuts at: 589 589
Size: 3575
SalI G'TCGA_C
Cuts at: 612 612
Size: 3575
SapI GCTCTTCn'nnn_
Cuts at: 473 1598 1808 473
Size: 1125 210 2240
Fragments arranged by size:
2240 1125 210
SbfI CC_TGCA'GG
Cuts at: 622 622
Size: 3575
ScaI AGT'ACT
Cuts at: 3092 3092
Size: 3575
SfcI C'TryA_G
Cuts at: 33 618 1303 2296 2487 33
Size: 585 685 993 191 1121
Fragments arranged by size:
1121 993 685 585 191
SfoI GGC'GCC
Cuts at: 1007 1255 1007
Size: 248 3327
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
SmaI CCC'GGG
Cuts at: 597 597
Size: 3575
SmlI C'TyrA_G
Cuts at: 160 730 1645 2137 2399 160
Size: 570 915 492 262 1336
Fragments arranged by size:
1336 915 570 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3575
SphI G_CATG'C
Cuts at: 628 1128 628
Size: 500 3075
StyI C'CwwG_G
Cuts at: 107 245 404 709 1063 107
Size: 138 159 305 354 2619
Fragments arranged by size:
2619 354 305 159 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1158 1291 1750 1959 1158
Size: 133 459 209 2774
Fragments arranged by size:
2774 459 209 133
TatI w'GTAC_w
Cuts at: 3090 3090
Size: 3575
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3543
Tth111I GACn'n_nGTC
Cuts at: 1372 1372
Size: 3575
XbaI T'CTAG_A
Cuts at: 606 606
Size: 3575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1070 1070
Size: 3575
XmaI C'CCGG_G
Cuts at: 595 595
Size: 3575
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BmtI BpmI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI