Restriction map for pF1KB6272
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AcsI        
AcyI        AflII       AflIII      AgeI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BciVI       BclI        
BcnI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsiWI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstUI       BstZ17I     CelII       
CfoI        ClaI        CpoI        DraIII      DrdI        
Eco47III    EcoO65I     EcoRI       FbaI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MfeI        MluI        MunI        MvnI        
NarI        NciI        NdeI        NheI        NotI        
NruI        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccIII       1505  TCCGGA               T^CCGGA
AclI         1088  AACGTT               AA^CGTT
AhdI          556  GACCTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         2133  GATCC                GGATC(4/5)
AlwNI        1062  CAGCTACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          332  CCTTAGG              CC^T[ACGT]AGG
ApaI         1521  GGGCCC               GGGCC^C
Asp700I       716  GAACCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         556  GACCTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI           33  GGTGCC               G^G[CT][GA]CC
BbvCI        1112  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1285  GCACTTGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1948  AGATCT               A^GATCT
BsaBI         961  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1505  TCCGGA               T^CCGGA
BsiEI        2079  CGGCCG               CG[GA][CT]^CG
BspEI        1505  TCCGGA               T^CCGGA
BspMI        1871  ACCTGC               ACCTGC(4/8)
BsrBI        2008  GAGCGG               CCGCTC(-3/-3)
BstAPI        871  GCAAGCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         824  TTCGAA               TT^CGAA
BstYI        1948  AGATCT               [GA]^GATC[CT]
Bsu36I        332  CCTTAGG              CC^T[ACGT]AGG
DraI          118  TTTAAA               TTT^AAA
EagI         2079  CGGCCG               C^GGCCG
Eam1105I      556  GACCTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2079  CGGCCG               C^GGCCG
Eco52I       2079  CGGCCG               C^GGCCG
Eco81I        332  CCTTAGG              CC^T[ACGT]AGG
EcoRV        1924  GATATC               GAT^ATC
FseI         2268  GGCCGGCC             GGCCGG^CC
MaeII        1089  ACGT                 A^CGT
MamI          961  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1948  AGATCT               [GA]^GATC[CT]
MroI         1505  TCCGGA               T^CCGGA
MseI          119  TTAA                 T^TAA
MspA1I       1382  CAGCTG               C[AC]G^C[GT]G
NspV          824  TTCGAA               TT^CGAA
PaeR7I        168  CTCGAG               C^TCGAG
Psp1406I     1088  AACGTT               AA^CGTT
PvuII        1382  CAGCTG               CAG^CTG
ScaI          746  AGTACT               AGT^ACT
SfuI          824  TTCGAA               TT^CGAA
SphI         1793  GCATGC               GCATG^C
Tru9I         119  TTAA                 T^TAA
XbaI         1393  TCTAGA               T^CTAGA
XhoI          168  CTCGAG               C^TCGAG
XhoII        1948  AGATCT               [GA]^GATC[CT]
XmnI          716  GAACCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AspHI         987  GAGCTC               G[AT]GC[AT]^C
             2074  GAGCTC              
AvaI          168  CTCGAG               C^[CT]CG[GA]G
             2030  CCCGAG              
BalI          963  TGGCCA               TGG^CCA
             2018  TGGCCA              
BbsI          457  GAAGAC               GAAGAC(2/6)
             1133  GTCTTC              
BpuAI         457  GAAGAC               GAAGAC(2/6)
             1133  GTCTTC              
BsaWI        1505  TCCGGA               [AT]^CCGG[AT]
             1722  TCCGGT              
BsgI           92  GTGCAG               GTGCAG(16/14)
             2089  GTGCAG              
BsiHKAI       987  GAGCTC               G[AT]GC[AT]^C
             2074  GAGCTC              
BsmBI        1418  CGTCTC               CGTCTC(1/5)
             2291  GAGACG              
BsoBI         168  CTCGAG               C^[CT]CG[GA]G
             2030  CCCGAG              
BstXI         266  CCAGCACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1155  CCATCGTAGTGG        
DsaI          800  CCATGG               C^C[GA][CT]GG
             1592  CCATGG              
EcoNI        1783  CCTTACAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2136  CCTCCACCAGG         
EcoT22I       307  ATGCAT               ATGCA^T
             1558  ATGCAT              
FauI         1241  GCGGG                CCCGC(4/6)
             2043  GCGGG               
HgaI         2293  GACGC                GACGC(5/10)
             2037  GCGTC               
MluNI         963  TGGCCA               TGG^CCA
             2018  TGGCCA              
MscI          963  TGGCCA               TGG^CCA
             2018  TGGCCA              
NaeI         1249  GCCGGC               GCC^GGC
             2269  GCCGGC              
NcoI          800  CCATGG               C^CATGG
             1592  CCATGG              
NgoMIV       1249  GCCGGC               G^CCGGC
             2269  GCCGGC              
NsiI          307  ATGCAT               ATGCA^T
             1558  ATGCAT              
SacI          987  GAGCTC               GAGCT^C
             2074  GAGCTC              
SexAI         735  ACCTGGT              A^CC[AT]GGT
             1715  ACCAGGT             
SfcI         1068  CTGTAG               C^T[GA][CT]AG
             1807  CTACAG              
SmlI          168  CTCGAG               C^T[CT][GA]AG
             2101  CTCAAG              
StuI          781  AGGCCT               AGG^CCT
             1409  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BanII       
BbvI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrFI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MnlI        MslI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        
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